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34 articles for “enzyme assays”
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A Comparison of Rapid Card Test with Enzyme-Linked Immunosorbent Assay for the Detection of Hepatitis B Surface Antigen [HBsAg] in Tertiary Care Hospital
Abstract: Introduction: Hepatitis B virus (HBV) infection is one of the leading causes of death worldwide. The most important marker for HBV infection is HBsAg. In case of diagnosis of infectious disease, discordant results may have serious consequences among the patients as it causes unnecessary mental stress and tension. For proper diagnosis of infection as well as disease management and prevention, identification of appropriate test kit is necessary. Aims and Objective: …
Published in Research and Reviews: A Journal of Microbiology and Virology · Vol. 7, Issue 3, 2017 · pp. 27–31 Read article
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Lab Reagents and Their Importance in Bio-chemistry
Abstract: Biochemical reagents are essential substances in the study of life at the molecular level. These reagents help scientists detect, identify, and quantify bio-molecules such as proteins, carbohydrates, nucleic acids, and enzymes in biological systems. They play a vital role in understanding metabolic reactions and physiological processes within living organisms. Biochemical reagents are generally classified into analytical, diagnostic, enzymatic, chromogenic, and buffer reagents, each serving a specific purpose in laboratory and …
Published in Research and Reviews : A Journal of Biotechnology · Vol. 16, Issue 1, 2026 · pp. 1–4 Read article
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Molecular Identification and Characterization of a Rice Field Cyanobacterium for its Possible Use as Biofertilizer in Acidic Environment
Abstract: Normal 0 false false false EN-US X-NONE X-NONE /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Table Normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-priority:99; mso-style-qformat:yes; mso-style-parent:""; mso-padding-alt:0in 5.4pt 0in 5.4pt; mso-para-margin:0in; mso-para-margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:11.0pt; font-family:"Calibri","sans-serif"; mso-ascii-font-family:Calibri; mso-ascii-theme-font:minor-latin; mso-fareast-font-family:"Times New Roman"; mso-fareast-theme-font:minor-fareast; mso-hansi-font-family:Calibri; mso-hansi-theme-font:minor-latin; mso-bidi-font-family:"Times New Roman"; mso-bidi-theme-font:minor-bidi;} Initial screening for cyanobacterial diversity in rice fields of Meghalaya revealed presence of a limited number of species predominantly belonging to heterocystous cyanobacteria such as Nostoc and Anabaena. …
Published in Research and Reviews: A Journal of Microbiology and Virology · Vol. 1, Issue 3, 2011 Read article
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Evaluation of Diagnostic Sensitivity of Rapid Immuno-Chromatographic Card Test (ICT) Against IgM ELISA for Serological Diagnosis of Scrub Typhus: An Experience at a Tertiary Care Centre in South-Eastern Rajasthan
Abstract: Introduction: Scrub typhus (ST) is a common but under-reported rickettsial disease in rural/sub-urban population of our country often resulting in severe complications and high mortality. Evaluation of ‘point of care’ tests like Rapid Immuno-Chromatographic Card Test (ICT) for diagnosis of ST is important to ascertain its utility in diagnosis of the disease in remote and peripheral settings. Aims: The present study was designed to evaluate the diagnostic sensitivity of Rapid …
Published in Research and Reviews: A Journal of Microbiology and Virology · Vol. 9, Issue 1, 2019 · pp. 85–91 Read article
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Development of a Polymeric Detection System for Salivary Annexin-1: A Potential Tool for Point-of-Care Diagnostics in Periodontal Disease
Abstract: Salivary annexin-1, a protein with anti-inflammatory properties, has emerged as a potential biomarker for periodontal disease. However, current detection methods, like ELISA, are often complex and require laboratory settings. This study explores the development of a novel polymeric detection system for the rapid and sensitive identification of salivary annexin-1. This system could leverage the unique properties of polymers to create a point-of-care diagnostic tool for: (1) early detection and monitoring …
Published in Journal of Polymer & Composites · Vol. 12, Issue 5, 2024 · pp. 101–110 Read article
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Variation in Activity of Lipase Immobilized on Chitosan and Alginate Nanoparticles by Changing Concentration of the Preparatory Reagents
Abstract: Chitosan and alginate nanoparticles were prepared by ionic gelation method. The concentrations of each of the preparatory reagents (for chitosan: acetic acid, chitosan, sodium sulphate, glutaraldehyde and for alginate: calcium chloride, alginate, glutaraldehyde) were altered to check their consequences. Lipase was chemically crosslinked to these nanoparticles. Enzymatic assay was carried out for every alteration separately. To visualize the changes in each reagent at every step a precise table is incorporated …
Published in Research and Reviews : A Journal of Life Sciences · Vol. 7, Issue 3, 2017 · pp. 14–22 Read article
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Killer of the Killer—Myeloperoxidase Inhibitor from Siddha System of Medicine
Abstract: Two proprietary siddha medicines such as NiKu plus and S.I.V.A. herbal drops were evaluated for myeloperoxidase (MPO) inhibition by in vitro enzyme assay. The MPO plays an important role in the predisposition of various complications both in diabetes mellitus (DM) and in psoriasis. Both NiKu plus and S.I.V.A. herbal drops showed high inhibitory activity of MPO. The findings suggest that regular use of NiKu and S.I.V.A. is must for patients …
Published in Research and Reviews: A Journal of Pharmacology · Vol. 8, Issue 3, 2018 · pp. 31–34 Read article
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Characterization of Laccase Enzyme from Dye Degrading Bacterial Strains Isolated from Natural Sources
Abstract: The continuous growth in globalization and industrialization has increased the environmental pollution and one such major contaminant is various types of synthetic dyes. Microbial decolorization and degradation of these dyes through secretion of various extracellular enzymes is gaining importance owing to their eco-friendly nature. One such enzyme is laccase, a multicopper oxidase enzyme which has the ability to oxidize both phenolic and non-phenolic compounds through reduction of molecular oxygen to …
Published in Research and Reviews: A Journal of Microbiology and Virology · Vol. 9, Issue 1, 2019 · pp. 65–76 Read article
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Comparative evaluation of Chromogranin A levels in GCF and saliva in healthy, chronic periodontitis and aggressive periodontitis patients – A Descriptive Co-relational study.
Abstract: Periodontal destruction is a combination of complex physiopathological process. Stress acts in the body by releasing stress hormones, the glucocorticoids and the catecholamines. Chromogranin A is a secretory glycoprotein which can be used as a biomarker. Aims: The aim of the study was to evaluate levels of chromogranin A in gingival crevicular fluid (GCF) and saliva in healthy, chronic periodontitis and aggressive periodontitis patients and to further correlate the clinical …
Published in Research and Reviews: A Journal of Dentistry · Vol. 14, Issue 1, 2023 · pp. 28–32 Read article
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Assessment of the Saliva and Serum Interleukine-6 in Patients with Chronic Periodontitis: Case Controlled Study
Abstract: Abstract:Chronic periodontitis is a multifactorial disease characterized by an interaction between dental plaque antigen and components of the host defense system. Cytokines are considered to play a key role in the inflammation process in chronic periodontitis. The aim of the study was to assess the levels of Interleukin-6(IL-6) in saliva and serum of patients with chronic periodontitis and to compare them with the levels that observed in healthy subjects. This …
Published in Research and Reviews: A Journal of Microbiology and Virology · Vol. 5, Issue 1, 2015 · pp. 1–7 Read article
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In vitro Antioxidant Activity and Phytochemical Analysis of Different Polar Solvent Extracts Obtained from Zanthoxylum rhetsa (Roxb) Bark
Abstract: This study was carried out to evaluate the phytochemical and antioxidant effectiveness of methanolic extract and different fractions (n-butanol, ethyl acetate, chloroform and n-hexane) of Z. rhetsa bark. Phytochemical activity of Zanthoxhylum rhetsa (Roxb) bark extract alkaloids, flavonoids, tannins, steroids, saponins and antraquinones was carried out. Antioxidant scavenging enzyme profile assays were done and profiles were developed for validation. The results indicated that bark extract and fractions of Z. rhetsa …
Published in Research and Reviews : A Journal of Life Sciences · Vol. 5, Issue 3, 2015 · pp. 1–7 Read article
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Isolation and Characterization of Protease Producing Organism Bacillus Species from Slaughter House Waste
Abstract: The purpose of this investigation was to isolate protease producing bacteria from slaughter house waste collected in local area of Kaliakkavilai. The Bacillus sp. was identified by several biochemical and physiological tests. The effect of different production parameters such as pH, temperature, metal ions concentration and substrate concentration for protease production by the isolated bacterial strain were studied. The enzyme production was assayed in submerged fermentation with optimized parameters to …
Published in Research and Reviews: A Journal of Microbiology and Virology · Vol. 3, Issue 3, 2013 · pp. 8–14 Read article
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Effects of Gypsophila on Airway Inflammation in Guinea pig model of ovalbumin‐induced Asthma
Abstract: To look at how Gypsophlia (GSP) affects airway inflammation in a model of ovalbumin-induced asthma in guinea pigs (OVA).Methods: Using a random number table, sixty healthy female guinea pigs were split into six groups, each consisting of ten rats: the normal control, OVA, and GSP (0.45, 0.9, 1.8, and 3.6 g/kg). The ovalbumin and lipopolysaccharide sensitization approach was used to create the OVA guinea pig model. The GSP groups received …
Published in Research and Reviews: A Journal of Pharmacology · Vol. 14, Issue 1, 2024 · pp. 17–23 Read article
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The Role of Rubella Virus Infection among Women with Spontaneous Abortion in Al-Najaf Governorate
Abstract: Rubella virus could cause a spectrum of birth defects known as Congenital Rubella Syndrome (CRS) if the infection occurs within the first or second trimester of pregnancy. Repeated miscarriage is defined as a condition with two or more consecutive abortions before the 20–25 weeks of gestation. Rubella remains to be a severe public health problem in developing countries. Recurrent miscarriage is a multi-factorial disease, which occurs in approximately 1%–2% of …
Published in International Journal of Virus Studies · Vol. 3, Issue 2, 2026 · pp. 1–6 Read article
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Screening of Bone Mineral Density, Serum Calcium and Vitamin D Level in Hyperthyroid Females of North India
Abstract: Introduction: Osteoporosis is a disease which plays a role in causing fracture especially in females. Hormonal dysfunction may be one of the possible causes of osteoporosis. Hyperthyroidism has been known to increase the risk of fracture especially in women both pre- and postmenopausal. The relation between hyperthyroidism and bone mineral density (BMD) is still unclear. Therefore, the present study was carried out to assess the effects of hyperthyroidism on long …
Published in Research and Reviews: A Journal of Health Professions · Vol. 8, Issue 2, 2018 · pp. 49–56 Read article
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An Overview of ELISA Biochemistry: A Brief Evaluation
Abstract: ELISA, known as the enzyme-linked immunosorbent assay, is widely recognized as the most reliable immunoassay method. This technique is highly sensitive and serves the purpose of identifying and measuring various substances such as hormones, antibodies, antigens, glycoproteins, allergens, and proteins. The process involves the formation of antibody–antigen complexes that generate a detectable response, enabling quantification. In response to encountering foreign substances called "antigens," the immune system of an individual produces …
Published in Research and Reviews : A Journal of Immunology · Vol. 13, Issue 1, 2023 · pp. 9–19 Read article
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Drug Eluting Stents Alter the Inflammatory Biomarkers after One Week of Stent Implantation in Patients with Ischemic Heart Disease: Gender-Based Study
Abstract: Angioplasty is a successful antianginal therapy. Drugs coated on the surface of drug eluting stents (DESs) are potent antimitotic agents that strongly inhibit smooth muscle proliferation and inflammatory process. This study aimed to prove that DES are released into the circulation and induced anti-inflammatory effect which is gender based beyond the local effect on the coronary artery via determination of the circulating levels of interleukin-6 (IL-6) and high sensitivity C-reactive …
Published in Research and Reviews: A Journal of Medicine · Vol. 6, Issue 2, 2016 · pp. 1–8 Read article
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Recent Advances in Nanotechnology-Assisted Liquid Crystal Biosensors for Sensitive and Naked-Eye Detection of Antibiotics
Abstract: The widespread use of antibiotics in healthcare, veterinary medicine, agriculture, and aquaculture has resulted in increasing levels of antibiotic residues in food products and environmental samples, posing significant risks to human health and contributing to antimicrobial resistance. Conventional analytical methods, including high-performance liquid chromatography (HPLC), liquid chromatography–mass spectrometry (LC–MS), enzyme-linked immunosorbent assay (ELISA), and electrochemical techniques, offer excellent sensitivity but require sophisticated instrumentation, skilled personnel, and extensive sample preparation. In …
Published in International Journal of Crystalline Materials · Vol. 3, Issue 2, 2026 Read article
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Clinical Implications of Serum NT-pro Brain Natriuretic Peptides in Type1 Diabetes with Positive Antiviral Antibodies
Abstract: Subclinical cardiovascular disease is common in diabetes mellitus and is associated with an increased risk of cardiovascular events including heart failure. This study was aimed to assess the association of NT-proBNP as a marker of heart failure and antiviral antibodies in patients with type 1 diabetes mellitus (T1D). One hundred and forty-seven T1D patients (65 male and 82 female) and 24 (11 male and 13 female) non-diabetic control subjects were …
Published in Research and Reviews: A Journal of Medicine · Vol. 3, Issue 1, 2013 · pp. 12–16 Read article
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Subclinical Significant High Serum Immunoglobulin E Response to 20 Allergens: Age and Gender Based Study
Abstract: Background: Antigen-immunoglobulin E (IgE) reaction is an example of immediate allergic reactions. High serum IgE levels were reported in many diseases that linked to the multiple allergens. Objectives: This study aimed to measure the serum IgE against 20 allergens in patients who had a previous history of allergy due to unidentified precipitating factors. Methods: This retrospective study was conducted at the Al Zahraa Consultant Center for Allergy and Asthma that …
Published in Research and Reviews : A Journal of Immunology · Vol. 9, Issue 3, 2019 · pp. 1–6 Read article