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14 articles for “denaturation”
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DNA Binding and Cleavage Studies of Cobalt Complexes Containing Bioactive Mixed Ligands
Abstract: The complexes containing bioactive ligand of the type [Co(L1L2)] (PF6)2 complex (1) (where L1=1,10-phenanthroline and L2 = N,N-dimethyl-2-{5-[(pyrrolidin-1-ylsulfonyl)methyl]-1H-indol-3-yl}ethanamine (almotriptan) in 1:1 molar ratio of ligands) and [Co(L1L3L4)] (PF6)2 complex (2) (where L3=4H-1,2,4-triazol-4-amine, L4=1H-benzimidazole-2-thiol and the ligands are in the ratio 1:1:1, were synthesized and characterized. The DNA binding property of the complexes with calf thymus DNA has been investigated using absorption spectra, viscosity measurements and thermal denaturation experiments. The intrinsic …
Published in Emerging Trends in Chemical Engineering · Vol. 1, Issue 1, 2014 · pp. 49–56 Read article
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Heat induced changes in casein
Abstract: In current times, dairy sectors involve milk being always subjected to heat treatment; main examples are: high temperature-short time (72 ˚C × 15 sec) pasteurization, low temperature-long time pasteurization (65 ˚C × 30 min), ultra-high temperature sterilization (140 ˚C × 5 sec), thermization (65 ˚C × 15 sec), in-container sterilization (112 ˚C × 15 min). Calcium chelators are, however, capable of increasing the heat stability of milk. Milk is considered …
Published in Research and Reviews : Journal of Dairy Science and Technology · Vol. 11, Issue 3, 2022 · pp. 1–5 Read article
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Cloning and Expression of LPS-Binding Single Domain Antibody Clones Selected from Phage Display Library of Indian Desert Camel
Abstract: Despite the absence of light chains in their structure, camelid and shark “heavy chain antibodies” (HCAbs) variable domains (designated as VHH) are able to make functional paratopes. The authors have selected lipopolysaccharide (LPS)-binder “single-domain antibodies” (dAb) clones from the previously constructed phage display library of (LPS)-immunized Indian desert camel. This study was undertaken to make available large amounts of some LPS-binder dAb clones by using a suitable expression vector-host system …
Published in Research and Reviews : A Journal of Immunology · Vol. 4, Issue 1, 2014 · pp. 1–7 Read article
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Synthesis, Characterization, and Biomedical Applications of a Silver Nanoparticle-Coated Chitosan–Fucoidan–Polymer Nanocomposite Loaded with Persea americana Leaf Extract
Abstract: Consuming a natural composite of Persea americana (Avocado) leaf extract and a chitosan and fucoidan form a biodegradable biopolymer matrix–polymer nanocomposite with biomedical applications, this study explored a potential therapeutic method. GC–MS and UV spectroscopy were used to describe the aqueous leaf extract, which was verified by phytochemical screening to contain flavonoids, tannins, phenols, terpenoids, and alkaloids. The chitosan–fucoidan biodegradable polymer matrix served as a biocompatible stabilizing and encapsulating carrier …
Published in Journal of Polymer & Composites · Vol. 14, Issue 5, 2026 · pp. 130–148 Read article
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In vitro antioxidant Anti-inflammatory and Cytotoxicity Activities from Hexane Extract of Bryonopsis laciniosa Fruits
Abstract: Bryonopsis laciniosa also known as “Shivlingi” annual climber with bright red fruits and is reported to be highly medicinal in India. As a folk medicine, the plant is used in treatment of broad range of diseases and disorders. In the present study, Hexane extract of B. laciniosa fruits were used to evaluate in vitro anti-inflammatory, antioxidant and Cytotoxicity (towards MCF-7 cell line) activities. In vitro anti-inflammatory activity by inhibition of …
Published in Research & Reviews : Journal of Botany · Vol. 7, Issue 1, 2018 · pp. 1–8 Read article
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Phyto-chemical Screening, In Vitro Anti-arthritic Study, Formulation and Evaluation of Ethanolic Extract of Helicteres Isora Fruits
Abstract: The present investigation was aimed at exploring the anti-arthritic activity of ethanolic extract of Helicteres isora fruits, using Bovine serum albumin (BSA) and Egg albumin denaturation method. The extract was subjected to preliminary phyto-chemical screening in order to find out the major phyto-constituents present in it. Various concentrations of the extract (100, 250, and 1000 μg/ml) were prepared and the percentage inhibition of protein denaturation was determined. Diclofenac sodium (100, …
Published in Research and Reviews: A Journal of Pharmacology · Vol. 10, Issue 1, 2020 · pp. 34–40 Read article
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A Short Review on Application of Fish Proteins in Functional Foods
Abstract: Functional foods contain specific nutrients that have beneficial physiological benefits, such as lowering the risk of chronic diseases and improving overall health. Functional foods are created by adding additives that have been shown to enhance health. This review article investigates several fish-derived products as functional dietary components, with an emphasis on fish protein hydrolysates (FPH), fish protein concentrate (FPC), fish collagen, fish gelatine, fish enzymes, fish protein powder (FPP) and …
Published in International Journal of Marine Life · Vol. 1, Issue 2, 2024 · pp. 7–16 Read article
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Scientific Proof for ‘Seeing is Believing’ of Keshraksha Gel
Abstract: The present study discusses about the HPTLC confirmation of the herbs used in the Siddha drug – Keshraksha gel. The study findings clearly confirm that all the herbs are well preserved in the gel base without any disintegration or denaturation. Assuming the therapeutic value of all the herbs used the well preserved status of these herbs in gel base validate their drug efficacy. Findings are discussed in the paper. Keywords: …
Published in Journal of AYUSH: Ayurveda, Yoga, Unani, Siddha and Homeopathy · Vol. 6, Issue 3, 2017 · pp. 15–18 Read article
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A Single-domain Antibody Cross-reacts with Lipopolysaccharide from Diverse Gram-negative Bacteria
Abstract: Gram-negative bacterial sepsis and endotoxemia are the leading causes of morbidity and mortality in animals and humans worldwide, and attempts have continued to design rational therapies. Endotoxin/lipopolysaccharide (LPS)-neutralizing polyclonal and monoclonal antibodies have been produced, but are not free from problems. Single-domain antibodies (dAbs), constructed from the camelid and the shark ‘heavy chain antibodies’ by phage display technology possess favorable features for in vivo applications. The objective of this study …
Published in Research and Reviews : A Journal of Immunology · Vol. 6, Issue 2, 2016 · pp. 29–37 Read article
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Evaluation of Antibacterial, Antioxidant, in vitroAnti-inflammatory and Cytotoxicity Activity on MCF-7 Cell Line from Methanol Extract of BryonopsislaciniosaFruits
Abstract: Bryonopsislaciniosa, a shrub known as “Shivlingi” in India, is neighbourly used as an apprentice medicine and tonic. As a folk medicine, it is used to treat a broad range of diseases and disorders. With these properties in mind, we have designed an experiment to evaluatethe antibacterial, in vitro antioxidant, anti-inflammatory and cytotoxicity activities of methanol extracts. The methanol extract was evaluated for antibacterial activity by agar well diffusion method, antioxidant …
Published in Research & Reviews : Journal of Botany · Vol. 7, Issue 1, 2018 · pp. 9–21 Read article
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Synthesis, Characterization and Biological Evaluation of Some Novel Phenazine Derivatives
Abstract: Novel phenazine analogues (PZ1‑PZ6) were synthesized, characterized by the spectral analysis and screened for anti-arthritis activity by bovin serum and egg albumin protein denaturation method. The synthetic route involves the equimolar mixture of ortho-phenylenediamine and ammonium persulphate which were dissolved in 1 M HCl to give 2,3-diaminophenazine(I). Different substituted aromatic aldehydes were added to the 2,3-diaminophenazine(I) and dissolved in methanol. The reaction mixture was stirred for 1‑2 h and then …
Published in Research & Reviews: A Journal of Drug Design & Discovery · Vol. 6, Issue 3, 2019 · pp. 31–37 Read article
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Preparation, Phytochemical Screening and Pharmacological Evaluation of Poly-herbal Formulation
Abstract: AbstractThe current investigation was endeavored at formulating and evaluating a poly-herbal formulation. The poly-herbal formulation was a combination of 10 herbal ingredients which was phyto-chemically screened for the detection of various phyto-constituents. The formulation was subjected to in vitro Anti-Arthritic and Anthelmintic activity studies. Anti-Arthritic activity was evaluated using various concentrations of the formulation (100 μg/ml, 250 μg/ml, and 500 μg/ml) and Diclofenac sodium (100 μg/ml, 250 μg/ml, and 500 …
Published in Research and Reviews: A Journal of Pharmacology · Vol. 10, Issue 2, 2020 · pp. 9–14 Read article
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HPTLC Confirmation of Lumina Herbal Shampoo
Abstract: The present study discusses about the HPTLC confirmation of the herbs used in the Siddha drug Lumina herbal shampoo. The study findings clearly confirm that all the herbs are well preserved in the shampoo base without any disintegration or denaturation. Assuming the therapeutic value of all the herbs used, the well preserved status of these herbs in Lumina Shampoo base validates their drug efficacy. Findings are discussed in the paper.Keywords: …
Published in Research and Reviews: A Journal of Pharmaceutical Science · Vol. 8, Issue 3, 2017 · pp. 17–20 Read article
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HPTLC Validation for Psorolin Medicated Bathing Bar
Abstract: AbstractThe present study discusses about the HPTLC confirmation of the herbs used in the Siddha product, Psorolin medicated bathing bar. The study findings clearly confirm that all the herbs are well preserved in the soap base without any disintegration or denaturation. Assuming the therapeutic value of all the herbs used, the well preserved status of these herbs in soap base validates their drug efficacy. Findings are discussed in the paper. …
Published in Research and Reviews: A Journal of Pharmaceutical Science · Vol. 9, Issue 1, 2018 · pp. 18–21 Read article