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248 articles for “assay”
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Biochemical Assays of Crude and Purified Oxycarotenoid Extracts Isolated from Coriander Leaves and their Effect on Oxidative Stability of Oils by Rancimat Assay
Abstract: Antioxidants are important substances in the fight against the damage done by free radicals that are produced by oxidative stress. Antioxidants are available in various forms as dietary supplements/synthetic forms. Because of their toxicity and side effects, the extensive use of many of these synthetic antioxidants is ruled out. Most nutritionist agrees that, the natural food is the best source of antioxidants. Dietary antioxidants have the ability to scavenge free …
Published in Research and Reviews : A Journal of Biotechnology · Vol. 8, Issue 1, 2018 · pp. 1–10 Read article
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Development and Validation of Liquid Chromatography-Tandem Mass Spectrometric Method for Assay of Zidovudine in Human UrineDevelopment and Validation of Liquid Chromatography-Tandem Mass Spectrometric Method for Assay of Zidovudine in Human Urine
Abstract: A sensitive and selective liquid chromatographic-tandem mass spectrometric (LC-MS-MS) method was developed to determine zidovudine (ZDV) in human urine. After solid-phase extraction with SPE cartridge, the urine sample was analyzed on a C18 column (Symmetry 3.5 µm; 75×4.6 mm i.d) interfaced with a triple quadrupole tandem mass spectrometer. Positive electrospray ionization was employed as the ionization source. The mobile phase consisted of water-acetonitrile (20:80, v/v).The method was linear over a …
Published in Research and Reviews: A Journal of Pharmaceutical Science · Vol. 5, Issue 3, 2014 · pp. 17–23 Read article
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Loop-Mediated Isothermal Amplification Coupled Lateral Flow Assay to Detect Nucleic Acids in Clinical Samples for Brucella Species at Point of Care and Resource-Limited
Abstract: Brucellosis is a most common and neglected zoonotic disease caused by Brucella species. Brucellosis is a major public health challenge as both animals and humans are affected. Most of the Brucella diagnostic tools are focused around serological assays though these assays cannot detect active pathogenesis. Loop-mediated isothermal amplification (LAMP) coupled with lateral flow assay (LFA) was developed in the present study to detect IS711 gene of Brucella species in the …
Published in Research and Reviews : A Journal of Life Sciences · Vol. 10, Issue 2, 2020 · pp. 35–42 Read article
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Titrimetric and Spectrophotometric Assay of Diethylcarbamazine Citrate in Pharmaceuticals using Permanganate as Oxidant
Abstract: Two titrimetric and one spectrophotometric methods are described for the determination of diethylcarbamazine citrate in bulk drug and dosage forms using permanganate as an oxidimetric agent. In method A, the drug solution in H2SO4 is titrated directly at 800C to a pink end point. DEC was treated with a measured excess of standard permanganate in H2SO4 medium, and after a contact time of 5 min, the residual oxidant back titrated …
Published in Research and Reviews: A Journal of Pharmaceutical Science · Vol. 7, Issue 3, 2016 · pp. 1–11 Read article
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Radio-Assay of Plant Samples at the Proposed Nuclear Reactor Site in Geregu, North-Central Nigeria
Abstract: Radio-assay of plant samples at the Geregu site, North-Central Nigeria, was evaluated to determine the radiological burden on the environment. Plant samples were collected from ten different locations on the site and the concentrations were measured using gamma spectroscopy. Radiological equivalent dose was estimated using standard analytical methods. The mean value for the external outdoor absorbed gamma dose rates due to terrestrial γ-rays from the nuclides Ra-226, Th-232 and K-40 …
Published in Journal of Nuclear Engineering & Technology · Vol. 5, Issue 3, 2015 · pp. 6–13 Read article
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Pharmaceutical Standardization and Preliminary assay of Kokilaksha (Asteracantha longifolia nees.) Ghana
Abstract: Introduction: Kokilaksha (Asteracantha longifolia Nees.) is an annual herb belonging to the acanthaceae family. In classics, Kokilaksha Kwatha (Decoction) is stated in the treatment of Nidranasha (Insomnia), Vatarakta (Gout), Shoola (Pain) and many more. Panchavidha Kashaya Kalpana (five basic preparation) are primary potent preparations of Ayurved pharmaceutics from which several additional preparations are developed as per need of time like Vati (Pills), Avaleha (Lincus), Ghana (Solidified aqueous extract), Kshara (Alkali) …
Published in Research & Reviews : Journal of Herbal Science · Vol. 12, Issue 3, 2023 · pp. 21–29 Read article
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Evaluation of Gamma Radiation Induced DNA Damage in Bombyx mori, using Single Cell Gel Electrophoresis (Comet Assay)
Abstract: Bombyx mori (B. mori) is the model insect used in the present study, which is commercially important compared to other Lepidopterans. Eggs of late embryonic stage (7 day old) of B. mori were exposed to different doses of gamma radiation to study the extent of DNA damage using comet assay which is a rapid technique to study the genotoxic effects. Test samples were exposed to varied doses of radiation (100, …
Published in Research and Reviews: A Journal of Toxicology · Vol. 11, Issue 1, 2024 · pp. 1–6 Read article
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GeneXpert MTB/RIF Assay (PCR Mycobacterium Tuberculosis): A Revolution in Management of Tuberculosis
Abstract: Background: Workup of pulmonary tuberculosis depends upon sputum microscopy. Sputum microscopy is less sensitive, human resource dependent and time consuming. Sometimes, it lead us to a delay in the diagnosis and may result in the spread of the disease. This seriously disturbs TB control program. There is a need for a more sensitive method. Similarly, early detection of rifampicin resistance is also of utmost importance to prevent MDR strains from …
Published in Research and Reviews : A Journal of Immunology · Vol. 8, Issue 2, 2018 · pp. 8–12 Read article
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Colorimetric Redox Indicator assay for rapid detection of multidrug-resistant Mycobacterium tuberculosis in South Gujarat region
Abstract: Tuberculosis (TB) has affected humans since antiquity. The burden of TB particularly with multi-drug-resistance (MDR) is increasing worldwide and has become a major public health concern. This study aims to perform Colorimetric Redox Indicator (CRI) assay for rapid detection of multidrug-resistant Mycobacterium tuberculosis in South Gujarat region. Total 234 samples were collected from South Gujarat region and were subjected for microscopy examination, culturing on Lowenstein-Jensen (LJ) medium and drug susceptibility …
Published in Research and Reviews: A Journal of Microbiology and Virology · Vol. 8, Issue 3, 2018 · pp. 65–70 Read article
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Multiplex PCR Assay for Rapid Detection of Different Strains of Escherichia coli Associated with Fish and Fishery Products
Abstract: A multiplex PCR (MPCR) assay was developed for the detection of Escherichia coli using selected primers designated on the species- and strains-specific genes such as hlyA, LT1 and phoA. The internal regions amplified were having a product size of 165, 275, and 903 bp, respectively. This MPCR assay had a sensitivity of detecting four cells of E. coli within 10 h in artificially contaminated shrimp tissue homogenate. The MPCR assay …
Published in Research and Reviews : A Journal of Life Sciences · Vol. 4, Issue 3, 2014 · pp. 1–9 Read article
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A Comparison of Rapid Card Test with Enzyme-Linked Immunosorbent Assay for the Detection of Hepatitis B Surface Antigen [HBsAg] in Tertiary Care Hospital
Abstract: Introduction: Hepatitis B virus (HBV) infection is one of the leading causes of death worldwide. The most important marker for HBV infection is HBsAg. In case of diagnosis of infectious disease, discordant results may have serious consequences among the patients as it causes unnecessary mental stress and tension. For proper diagnosis of infection as well as disease management and prevention, identification of appropriate test kit is necessary. Aims and Objective: …
Published in Research and Reviews: A Journal of Microbiology and Virology · Vol. 7, Issue 3, 2017 · pp. 27–31 Read article
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Preliminary Anticancer Study of Dendrophthoe trigona and Dendrophthoe falcata using Brine Shrimp Lethality Assay
Abstract: Hemiparasite plants, such as mistletoe, obtains some nourishment from its host and is also photosynthetic to some degree. Despite of their dependence on the host, they are used in treatment of arthritis, urinary disorders, inflammation. The present investigation was aimed at investigating preliminary anticancer potential of ethanolic extract of Dendrophthoe trigona Danser, Loranthaceae and Dendrophthoe falcata Danser, Loranthaceae parasite on Ficus racemosa Linn. Moraceae and Dalbergia paniculata, Fabaceae, respectively. The …
Published in Research & Reviews: A Journal of Pharmacognosy · Vol. 1, Issue 3, 2014 · pp. 19–21 Read article
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Study the Effect of Variation of Crude Assay on the Design of Distillation Column
Abstract: The purpose of this study is to investigate the effect of variation of crude assay on the design of crude distillation column. Different crudes assay (six samples of Sudanese crude oil) with different properties which are processed at the Khartoum Refinery Company have been simulated using Aspen HYSYS under the same operating conditions in order to identify the effect of this variation. Energy consumption optimization is done by using preheated …
Published in Journal of Petroleum Engineering & Technology · Vol. 6, Issue 3, 2016 · pp. 61–76 Read article
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Evaluation of Marine Waste Water And Aromatic Hydrocarbons Toxicity Using A Battery of Assays
Abstract: Abstract The aim of this study is to evaluate the toxic effects of marine wastewater and representative aromatic hydrocarbons using a battery of assays. The methods employed for the toxicological evaluation involve physic-chemical analysis of the marine wastewater, toxi-chromo test using mutant E coli and sub-chronic animal toxicity testing with emphasis on body weight determination, biochemical indices and histopathological examination. The results showed that Nembe water side wastewater sample contain …
Published in Research and Reviews: A Journal of Toxicology · Vol. 10, Issue 2, 2020 · pp. 1–13 Read article
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Preliminary Phytochemical Analysis and Haemolytic Activity Assay of Tuber Extract of Ruellia tuberosa L.
Abstract: Presence of certain chemical compounds is responsible for medicinal properties of the plant. In the present study, the various biochemical tests were conducted to examine the presence of various phytochemicals in the methanolic, petroleum ether and chloroform tuber extracts of Ruellia tuberosa L. Also, the extracts were subjected to the in vitro haemolytic activity assay. The tests confirmed the presence of carbohydrates, alkaloids, tannins, terpenoids and flavanoids in all the …
Published in Research and Reviews : A Journal of Life Sciences · Vol. 7, Issue 3, 2017 · pp. 23–27 Read article
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Cytogenotoxicity Assessment of Asphalt Plant Discharge Water using Plant Assay
Abstract: The Allium cepa assay test was used to evaluate cytogenotoxic effects of asphalt plant discharge water at the concentrations of 10, 20, 30, 40, 50, 60, 70, 80, 90 and 100% (v/v) asphalt plant discharge water/distilled water. The distilled water served as the negative control. The analysis of the physicochemical properties and heavy metals concentrations showed that most parameters were higher than the established standards of WHO and FMENV. The …
Published in Journal of Water Pollution & Purification Research · Vol. 12, Issue 3, 2025 · pp. 100–110 Read article
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Genotoxicity Evaluation of Hospital Waste Waters: A Study Monitored by E.coli WP2 Assay
Abstract: The major sources of health-care waste are hospitals. A variety of substances such as pharmaceuticals, radionuclide and solvents are used in hospitals for medical diagnostics, disinfection and research work. After application, some of these substances and excreted non-metabolized pharmaceuticals by the patients enter into the hospital effluents. Indeed, some of these substances found in wastewaters are genotoxic and are suspected to be a possible cause of the cancers observed in …
Published in Research and Reviews: A Journal of Toxicology · Vol. 3, Issue 1, 2013 · pp. 6–11 Read article
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High-Performance Liquid Chromatographic Assay of Nateglinide and its Stability Study
Abstract: Nateglinide (NTG) is a D-phenylalanine derivative used in the treatment of type-2 diabetes mellitus. An accurate, sensitive and reproducible high-performance liquid chromatographic (HPLC) method has been developed and validated for the quantification of NTG in pharmaceutical samples. The drug was eluted from Inertsil ODS 3V (150×4.6 mm; 5 µm particle size) column at 30á´¼C with a mobile phase consisting of phosphate buffer of pH 3.3 and methanol (70:30 v/v). The …
Published in Research and Reviews: A Journal of Pharmaceutical Science · Vol. 7, Issue 3, 2016 · pp. 41–52 Read article
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Simple, Direct and Stability-Indicating UV Spectrophotometric Assay of Pioglitazone Hydrochloride in Pharmaceuticals
Abstract: Pioglitazone hydrochloride (PGH) is an antihyperglycemic agent used in the treatment of type-2 diabetes mellitus. A simple and straight forward UV-spectrophotometric method is presented for the determination of PGH in bulk drug and tablets by measuring the absorbance of drug solution in 0.1M H2SO4 at 269 nm. A plot of absorbance versus concentration obeyed Beer’s law over 2–36 µg ml-1 concentration range (r = 0.9981) with a molar absorptivity of …
Published in Research and Reviews: A Journal of Pharmaceutical Science · Vol. 8, Issue 3, 2017 · pp. 7–16 Read article
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Biological Synthesis of Silver Nanoparticles Using Hibiscus Sabdariffa Leaf Extract and It’s Antibacterial Sensitivity Assay
Abstract: In this study, AgNO3 was effectively used to synthesize silver nanoparticles. Hibiscus sabdariffa leaf extract was used to promptly and easily synthesize silver nanoparticles. An UV-Visible spectrophotometer showed absorbance peak in range of 300-600 nm. The procedure was found to be easy to follow, quick, least step and safe for the environment. It is non-toxic, and a viable substitute for traditional physical or chemical procedures. At room temperature, silver ions …
Published in International Journal of Tropical Medicines · Vol. 1, Issue 1, 2024 · pp. 08–13 Read article