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35 articles for “and LOQ”
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Metallic Impurities Present in Environmental Water Samples Were Analyzed at Trace Levels Using ICP-MS-KED
Abstract: Elemental impurities in environmental water samples must be carefully monitored since they may have unintended pharmacological and toxicological consequences. For the simultaneous determination of eight metals (Cr, Ni, Cd, Pb, Hg, As, Se, and Sb) in water by ICP-MS, an accurate and sensitive approach has been devised and validated.The method's validity is confirmed through assessment of various criteria for new techniques, including linearity and range, accuracy, precision, selectivity, limit of …
Published in Research and Reviews: A Journal of Pharmaceutical Science · Vol. 14, Issue 1, 2023 · pp. 53–58 Read article
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A Sensitive SPE-ECD-GLC Procedure for Analyzing Simazine Quantitatively in Food Products
Abstract: Short and simple solid-phase extraction (SPE) method interfacing and hyphenating gas liquid chromatographic-electron capture detection (GLC-ECD) method has been developed for the qualification and quantification of simazine in some daily used agro products. United States Pharmacopeia G-42 gas chromatography stationary phase with ZB-35a, HTb Inferno-cZ (Zebron, Za) capillary column with an enlarged and extended temperature range of –60° to 400°C and electron capture detector was selected correlating some chemical, physical …
Published in International Journal of Biochemistry and Biomolecule Research · Vol. 3, Issue 1, 2025 · pp. 20–27 Read article
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Analytical Method Development and Validation of Simultaneous Estimation of Natural Active Constituents Andrographolide and Piperine by UV Method.
Abstract: A simple, accurate, precise, and robust UV-Visible spectrophotometric method was developed and validated for the simultaneous estimation of Andrographolide and Piperine in bulk and combined pharmaceutical dosage forms. Preformulation studies confirmed favorable physicochemical properties for both drugs, including acceptable solubility profiles, melting points, pH values, and partition coefficients. The method development involved determination of λmax values at 224 nm for Andrographolide and 288 nm for Piperine using ethanol as solvent. …
Published in Research & Reviews: A Journal of Pharmacognosy · Vol. 12, Issue 3, 2025 · pp. 33–41 Read article
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Lamivudine And Dolutegravir Simultaneous Dosage Forms Determined and Validated Using The RP-UPLC Method
Abstract: RP-UPLC has been used to produce a straightforward, precise, and accurate approach for the simultaneous estimation of dolutegravir and lamivudine in a pharmaceutical dose form. A HSS C18 column of 2.8 x 50 mm and featuring particles with a size of 1.6 μm was used to process the chromatogram. A mixture of 70:30 Buffer Na2HPO4 and methanol made up the mobile phase, which was pushed across the column at a …
Published in Emerging Trends in Personalized Medicines · Vol. 1, Issue 1, 2024 · pp. 5–13 Read article
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Development and Validation of a Robust RP-HPLC Method for Rufinamide Quantification in Pharmaceutical Dosage Form
Abstract: The creation and validation of a simple, precise, and targeted reverse phase high-performance liquid chromatographic (RP-HPLC) approach allowed for the quantification of rufinamide in pharmaceutical dosage forms. With a mobile phase of methanol and water (50:50, v/v), the technique used a gradient mode Symmetry Qualisil gold C18 column (4.6 x 150mm, 5 μm). For the detection method, a flow rate of 1 mL/min was employed at 220 nm. During a …
Published in Emerging Trends in Personalized Medicines · Vol. 1, Issue 1, 2024 · pp. 25–32 Read article
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Method development and validation of RP-HPLC for simultaneous estimation of etoricoxib and Thiocolchicosidein tablet dosage forms
Abstract: Objective: A New approach became set up for simultaneous estimation of Etoricoxib and Thiocolchicoside with the aid of RP-HPLC approach.Methods: Chromatographic separations were carried using Agilent XDB C18, 150 x 4.6 mm, 5m, column with a mobile phase composition of Sodium Phospahte buffer 3.5 pH and Acetonitrile (20:80) Were delivered at a glide price of 1ml/min and the detection changed into done the usage of waters HPLC automobile sampler, separation …
Published in Journal of Modern Chemistry & Chemical Technology · Vol. 12, Issue 3, 2021 Read article
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Validation and Forced Degradation Studies for the Determination of Erlotinib in Bulk and Pharmaceutical Dosage Forms by RP-HPLC
Abstract: A new reverse phase-high performance liquid chromatography (RP-HPLC) have been developed and validated for the determination of Erlotinib in bulk drug and pharmaceutical dosage form. The developed method is rapid, accurate, precise, simple and economical. The separation was carried out using column Hypersil ODS C-18 (150 mm x 4.6 mm 5 μm particle size) in isocratic mode, using mobile phase composition of 0.1% orthophosphoric acid: Acetonitrile in the ratio of …
Published in Research & Reviews: A Journal of Drug Formulation, Development and Production · Vol. 3, Issue 2, 2016 · pp. 44–53 Read article
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HPTLC Method Development and Validation of Genistein and Daidzein Estimation in Mucuna cochinchinensis Seed Extracts
Abstract: Aim: The current study was designed with the objective to estimate the concentration of Genistein and Daidzein in ethyl acetate extract of Mucuna cochinchinensis by high-performance thin-layer chromatography (HPTLC).Methods: The separation of bioactive compounds was performed using mobile phase, Toluene: n-Butanol: Glacial Acetic Acid: Water (1:4:2:2) and detected at wavelength 254 nm. The method was validated for linearity, accuracy, precision, limit of detection (LOD) and limit of quantification (LOQ) by …
Published in Research & Reviews: A Journal of Drug Formulation, Development and Production · Vol. 7, Issue 1, 2020 · pp. 16–25 Read article
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Development and Validation of Spectrophotometric and Colorimetric Method for the Determination of Ritonavir in its Bulk and Pharmaceutical Dosage Form
Abstract: A straightforward, exact, precise and sensitive UV spectrophotometric assay technique and a basic and sensitive colorimetric assay method have been created and validated for the quantitative estimation of Ritonavir from bulk drug and tablet formulations. UV spectrophotometric strategy which includes the assurance of Ritonavir in bulk medication and pharmaceutical dosage forms and has an assimilation greatest at 240 nm in methanol. The Beer's law was obeyed over the concentration range …
Published in Trends in Drug Delivery · Vol. 4, Issue 3, 2017 · pp. 32–41 Read article
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A Novel UPLC Method Development and Validation for the Estimation of Paracetamol and Chlormezanone in Dosage Form
Abstract: A simple, rapid, specific, accurate and precise Ultra High-Performance Liquid Chromatographic (HPLC) method was developed for the estimation of paracetamol and chlormezanone in dosage forms. Water Acquity C18 (100×2.1 mm ID) 1.8 µm was the column used. Methanol: Water: TEA (Tri ethyl amine) in the ratio of 50:50:0.5% (v/v/v) was the optimized mobile phase and pH was adjusted to 7.5 by using Ortho Phosphoric Acid. The flow rate was 0.5 …
Published in Research and Reviews: A Journal of Pharmaceutical Science · Vol. 11, Issue 1, 2020 · pp. 1–8 Read article
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RP-HPLC Method for the Simultaneous Estimation of Hydrocodone and Pseudoephedrine in Pharmaceutical Dosage Form
Abstract: A simple, rapid, specific, accurate and precise high-performance liquid chromatographic method was developed for the estimation of Hydrocodone and Pseudoephedrine in pharmaceutical dosage forms. Phenomenex C18 (250×2.2 mm ID) 4.5 µm was the column used. Methanol: Water: TEA (Tri ethyl amine) in the ratio of 50:50:0.5% (v/v/v) was the optimized mobile phase and pH was adjusted to 7.5 by using ortho-phosphoric acid. The flow rate was 1.0 ml/min and effluents …
Published in Trends in Drug Delivery · Vol. 7, Issue 1, 2020 · pp. 17–24 Read article
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Simultaneous Estimation of Prazosin and Polythiazide in Bulk and Fixed Dose Combination (Tablets) by RP-HPLC
Abstract: Objective:A New method was established for simultaneous estimation of Prazosin and Polythiazide by RP-HPLC method. Methods:Chromatographic separations were carried using HPLC equipped with Auto Sampler and PDA Detector, Discovery (150 x 46 mm, 5mm) column with a mobile phase composition of Buffer and ACN: taken in the ratio 60:40 (60:40 v/v) have been delivered at a flow rate of 1ml/min and the detection was carried out using waters HPLC auto …
Published in Research and Reviews: A Journal of Pharmaceutical Science · Vol. 12, Issue 3, 2025 · pp. 66–74 Read article
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New RP-HPLC Method Development and Validation for Simultanious Estimation and Forced Degradation Studies of Gallic acid and Curcumin in Solid Dosage Form
Abstract: Objective: A New method was established for simultaneous estimation of Gallic acid and curcumin by RP-HPLC method. Methods: Chromatographic separations were performed on an Inspire (4.6 x 150mm, 5m) column with a mobile phase composition of 0.1 percent OPA buffer and Acetonitrile (30:70) delivered at a flow rate of 1 ml/min, and detection was performed on a waters HPLC auto sampler, separation module 2695 with PDA detector 2996 at wavelength …
Published in Research and Reviews: A Journal of Pharmaceutical Science · Vol. 13, Issue 1, 2022 · pp. 7–15 Read article
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A Concurrent Estimation of Phytoconstituents Piperine, Quercetin and Kaempferol Present in Siddha Polyherbal Formulation Kabasara Kudineer by Rp-Hplc
Abstract: A straightforward, accurate, and precise technique was developed and validated for the simultaneous determination of Quercetin, Kaempferol, and Piperine in the polyherbal formulation Kabasura Kudineer. The sample was chromatographed using a C18 column (150x4.6mm, 5u) with a mobile phase composed of Acetonitrile and Ortho phosphoric acid in a 45:55 ratio, flowing through the column at a rate of 1ml/min. The detection wavelength for Quercetin, Kaempferol, and Piperine was set at …
Published in International Journal of Antibiotics · Vol. 1, Issue 1, 2024 · pp. 1–9 Read article
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Development and validation of a method for simulataneous determination of tenovifir disoproxil fumarate emtriculabine and isonized in bulk and pharmaceutical dosage form using RP-HPLC with relevance to toxicological aspects
Abstract: Hence, proposed analytical methods are simple, novel, economical, rapid, sensitive, reproducible and accurate for the Simultaneous estimation of Tenofovir Disoproxil Fumarate, Emtricitabine and Isoniazid in Bulk and Phamaceutical dosage form by using RP- HPLC. This Method gives reliable assay results with short analysis time using mobile phase of Acetonitrile : 0.02M Potassium Dihydrogen Orthophosphate: water (pH 5.3) in the ratio of 60: 25: 15 respectively. Retention time was found to …
Published in Research and Reviews: A Journal of Toxicology · Vol. 14, Issue 1, 2024 · pp. 17–25 Read article
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A New Separation Technique for Method, Development and Validation of Aclidinium Bromide and Formoterol Fumarate in Its Pure and Pharmaceutical Dosage Form by Using Rp-HPLC
Abstract: A new simple, precise, selective and accurate, a new method of development and validation using Rp-HPLC for the estimation of Aclidinium bromide and Formoterol Fumarate in its pure and pharmaceutical dosage form. Chromatogram was run through DIKMA Spursil, C18 segment (4.6×150 mm, 5µ0). Mobile phases contain 0.1% OPA: Acetonitrile (30:70) with the use of the 0.1% OPA buffer, stream rate of 1 ml/min and measured in 280 nm. The run …
Published in Research and Reviews: A Journal of Pharmaceutical Science · Vol. 15, Issue 3, 2024 · pp. 18–36 Read article
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HPTLC Method Development and Validation for Rabeprazole Sodium in Pharmaceutical Formulation
Abstract: Background: The most common medication for acid related disorders stands as Proton pump inhibitor (PPI) Rabeprazole Sodium. The pharmaceutical formulations require essential quality control of Rabeprazole Sodium through reliable analytical methods to maintain reliability. HPTLC provides a cost-effective rapid method for drug quantification compared to conventional HPLC methods so how HPTLC stands as an appropriate method for drug determination. Objective: The emphasis of this research project involves developing and validating …
Published in Research and Reviews: A Journal of Pharmaceutical Science · Vol. 16, Issue 2, 2025 · pp. 18–24 Read article
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RP-HPLC Analytical Method Development and Validation for Quantitative Estimation of Metoclopramide Hydrochloride in Solid Oral Formulations
Abstract: RP-HPLC technique formulated for the numerical assessment of Metoclopramide Hydrochloride in tablet formulation has been confirmed for reliability. A C18 column facilitated the chromatographic separation using a mobile phase comprising 60:40 (v/v) potassium dihydrogen phosphate buffer: meth anol mixture. Such method was used to detect, where the retained time was 6.78 minutes and Metoclo pramide Hydrochloride produced a clear peaked at 273 nm. Results were in agreement with ICH Q2 …
Published in Research & Reviews: A Journal of Drug Formulation, Development and Production · Vol. 12, Issue 2, 2025 · pp. 07–15 Read article
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Analytical Method Development and Validation for Simultaneous Estimation of Paracetamol and Piroxicam by UV Method
Abstract: A new establishment and validation of a simple, precise, accurate and cost effective UV-Visible spectrophotometric method was developed to estimate both Paracetamol and Piroxicam simultaneously in conjoined pharmaceutical dosage forms. This technique was founded on simultaneous equation method of absorbances recorded at 252.0 nm and 282.0 nm of Paracetamol and Piroxicam in methanol respectively and an isosbestic point could be observed at 265.0 nm. It showed very good linearity over …
Published in Research & Reviews: A Journal of Drug Formulation, Development and Production · Vol. 12, Issue 3, 2025 · pp. 14–21 Read article
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Optimizing Chromatographic Techniques for Comprehensive Paraben Analysis to Enhance Safety in Consumer Products
Abstract: Parabens (PBs), such as methylparaben (MePB), ethylparaben (EtPB), propylparaben (PrPB), and butylparaben (BuPB), are widely used as preservatives in pharmaceuticals, food, and personal care products due to their antibacterial properties. However, there are growing worries about their potential to disrupt hormonal functions, which has led to stricter regulations. This study focuses on creating a robust high-performance liquid chromatography (HPLC) method for quickly measuring all four parabens in consumer goods. We …
Published in Journal of Polymer & Composites · Vol. 13, Issue 6, 2025 · pp. 228–243 Read article