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Research and Reviews: A Journal of Pharmaceutical Science

New RP-HPLC Method Development and Validation for Simultanious Estimation and Forced Degradation Studies of Gallic acid and Curcumin in Solid Dosage Form

  1. S. K. Godasu
  2. S. A. Sreenivas

Abstract

Objective: A New method was established for simultaneous estimation of Gallic acid and curcumin by RP-HPLC method. Methods: Chromatographic separations were performed on an Inspire (4.6 x 150mm, 5m) column with a mobile phase composition of 0.1 percent OPA buffer and Acetonitrile (30:70) delivered at a flow rate of 1 ml/min, and detection was performed on a waters HPLC auto sampler, separation module 2695 with PDA detector 2996 at wavelength 260 nm. Results: The retention time for Gallic acid and curcumin were 2.119 and 2.730 minute respectively. The linearity correlation coefficients for Gallic acid and curcumin were 0.999 and the concentration ranges were 500-2500 g/ml and 5-25 g/ml, respectively. For accuracy the total recovery was found to be 100.58% and 100.54% for Gallic acid and curcumin. LOD and LOQ for gallic acid 3.05 and 10.07. LOD and LOQ for Curcumin 2.28 and 9.98. Conclusion: The study's findings revealed that the suggested RPHPLC method is a simple, accurate, precise, rugged, robust, rapid, and repeatable method for routinely estimating Gallic acid and curcumin in pharmaceutical dose form.

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