STM Journals Peer-reviewed research from Consortium e-Learning Network

Research & Reviews: A Journal of Drug Formulation, Development and Production Original Research

Analytical Method Development and Validation for Simultaneous Estimation of Paracetamol and Piroxicam by UV Method

  1. Md. Adil Khan Department of Pharmacy, Millennium College of Pharmacy, Bhopal
  2. Ankita Shukla Department of Pharmacy, Millennium College of Pharmacy, Bhopal
  3. Bala Subramanyam Department of Pharmacy, Millennium College of Pharmacy, Bhopal

Abstract

A new establishment and validation of a simple, precise, accurate and cost effective UV-Visible spectrophotometric method was developed to estimate both Paracetamol and Piroxicam simultaneously in conjoined pharmaceutical dosage forms. This technique was founded on simultaneous equation method of absorbances recorded at 252.0 nm and 282.0 nm of Paracetamol and Piroxicam in methanol respectively and an isosbestic point could be observed at 265.0 nm. It showed very good linearity over concentration range of 5 30 UV/mL of both drugs with correlation coefficient (R 2 ) of 0.997 to Paracetamol and 0.992 to Piroxicam. Repeatability and reproducibility were ensured due to results of the precision studies with %RSD values that were within the acceptable range. The recovery experiments confirmed the accuracy and gave the values of recovery Paracetamol =78.9 and Piroxicam=88.6. The approach was harsh and hardy when there would be slight strained changes in the lab oratory conditions. The determined values of LOD and LOQ were 60.46 5g/mL and 194.32 5g/mL of Paracetamol and 57.98 5g/mL and 175.71 5g/mL of Piroxicam, respectively. The adopted procedure is easy, fast and reproducible in daily simultaneous determination of Paracetamol and Piroxicam in bulk drugs and co-formulated tablets, in accordance with ICH Q2 (R1) regulations.

Keywords

References (13)

  1. Professor and Head, Dept. of Pharmacognosy, The Erode College of Pharmacy, Perundurai Main Road, Veppampalayam, Erode-638112, Tamilnadu, India, RaviKKumar VR, Rathi S, Professor & Principal, School of Pharmacy, Rai University, Ahmedabad, Gujarat, India, Singh S, Assistant Professor, School of Pharmacy, Rai University, Ahmedabad, Gujarat, India, et al. A Comprehensive Review on Ulcer and Their Treatment. Chinese Journal of Applied Physiology. 2023;39:e20230006. doi:10.62958/j.cjap.2023.006
  2. Professor, Patel College of Pharmacy, Madhyanchal Professional University, Ratibad, Bhopal, MP. India, Rajput DS, Gupta N, Professor and Dean, Patel College of Pharmacy, Madhyanchal Professional University, Ratibad, Bhopal, MP. India, Singh S, Ph.D. Research Scholar, Patel College of Pharmacy, Madhyanchal Professional University, Ratibad, Bhopal, MP. India, et al. A Comprehensive Review: Personalized Medicine for Rare Disease Cancer Treatment. Chinese Journal of Applied Physiology. 2023;39:e20230008. doi:10.62958/j.cjap.2023.008
  3. Patel College of Pharmacy, Madhyanchal Professional University, Bhopal, MP. India, Singh S, Chaurasia A, Patel College of Pharmacy, Madhyanchal Professional University, Bhopal, MP. India, Rajput DS, Patel College of Pharmacy, Madhyanchal Professional University, Bhopal, MP. India, et al. Mucoadhesive Drug Delivery System and There Future Prospective: Are a Promising Approach for Effective Treatment? Chinese Journal of Applied Physiology. 2023;39:e20230005. doi:10.62958/j.cjap.2023.005
  4. Sigmapharm laboratories llc, Bensalem, Pennsylvania, USA, Patel S, Y I, Crescent School of Pharmacy, B.S. Abdur Rahman Crescent Institute of Science and Technology, Chennai-600048, Tamilnadu, India, Singh S, GSVM Medical College (MBBS 3rd year Student), Kanpur-208002, Uttar Pradesh, India, et al. Recent Innovations and Future Perspectives in Transferosomes for Transdermal Drug Delivery in Therapeutic and Pharmacological Applications. Chinese Journal of Applied Physiology. 2024:e20240031. doi:10.62958/j.cjap.2024.031
  5. Assistant Professor, Department of Pharmaceutical Sciences, Faculty of Health Sciences, Marwadi University, Rajkot, India, Vaghela MC, Rathi S, Professor and Principal, School of Pharmacy, Rai University, Ahmedabad, Gujarat, India, Shirole RL, Associate Professor, Department of Pharmacology, DCS’s A.R.A. College of Pharmacy, Nagaon, Dhule (MS) India, et al. Leveraging AI and Machine Learning in Six-Sigma Documentation for Pharmaceutical Quality Assurance. Chinese Journal of Applied Physiology. 2024;40:e20240005. doi:10.62958/j.cjap.2024.005
  6. Sigmapharm laboratories llc, Bensalem, Pennsylvania, USA, Patel S, Y I, Crescent School of Pharmacy, B.S. Abdur Rahman Crescent Institute of Science and Technology, Chennai-600048, Tamilnadu, India, Singh S, GSVM Medical College (MBBS 3rd year Student), Kanpur-208002, Uttar Pradesh, India, et al. Recent Innovations and Future Perspectives in Transferosomes for Transdermal Drug Delivery in Therapeutic and Pharmacological Applications. Chinese Journal of Applied Physiology. 2024:e20240031. doi:10.62958/j.cjap.2024.031
  7. Singh, S., Chaurasia, A., Rajput, D. S., & Gupta, N. (2024). An overview on mucoadhesive buccal drug delivery systems & approaches: A comprehensive review. African Journal of Biological Sciences (South Africa), 6(5), 522–541, doi:10.33472/AFJBS.6.5.2024.522-541
  8. Kumar S, Singh S, Rajput D, Sharma B, Chaturvedi K, Singh N, et al. Pharmacological Approaches and Herbal Interventions for Alzheimer's Disease. The Natural Products Journal. 2024;14(8). doi:10.2174/0122103155275266231123090138
  9. Department of Pharmacognosy, The Erode College of Pharmacy,Erode, Tamilnadu, India-638112, Ravikkumar VR, Patel BD, Department of Pharmaceutical Chemistry & Quality Assurance, School of Pharmacy, Rai University, Saroda, Dholka Road, Ahmedabad, Gujarat, India-382260, Rathi S, Department of Pharmaceutics, School of Pharmacy, Rai University, Saroda, Dholka Road, Ahmedabad, Gujarat, India-382260, et al. Formulation and Evaluation of Drumstick Leaves Tablet as An Immunomodulator. Chinese Journal of Applied Physiology. 2024;40:e20240004. doi:10.62958/j.cjap.2024.004
  10. Sharma, A., Bara, G., Keshamma, E., Sharma, B., Singh, S., Singh, S. P., Parashar, T., Rathore, H. S., Sarma, S. K., & Rawat, S. (2023). Cancer biology and therapeutics: A contemporary review. Journal of Cardiovascular Disease Research, 14(10), 1229-1247.
  11. Patel College of Pharmacy, Madhyanchal Professional University, Ratibad, Bhopal, MP. India, Singh S, Chaurasia A, Patel College of Pharmacy, Madhyanchal Professional University, Ratibad, Bhopal, MP. India, Gupta N, Patel College of Pharmacy, Madhyanchal Professional University, Ratibad, Bhopal, MP. India, et al. Effect of Formulation Parameters on Enalapril Maleate Mucoadhesive Buccal Tablet Using Quality by Design (QbD) Approach. Chinese Journal of Applied Physiology. 2024;40:e20240003. doi:10.62958/j.cjap.2024.003
  12. Patel SK, Prathyusha S, Kasturi M, Godse KC, Singh R, Rathi S, et al. Optimizing Irbesartan Fast Dissolving Tablets Using Natural Polysaccharides for Enhanced Drug Delivery and Patient Compliance. International Research Journal of Multidisciplinary Scope. 2025;06(01):1181-1190. doi:10.47857/irjms.2025.v06i01.02542
  13. Formulation, optimization and evaluation of mucoadhesive buccal tablets of ondansetron for enhanced bioavailability and sustained drug release. Biochemical and Cellular Archives. 2025;25(1). doi:10.51470/bca.2025.25.1.1063
Support