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649 articles for “isolate”
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Isolation of Bioactive Molecules from Ficus racemosa – Its Biochemical Characterization and in silico Analysis of Antimicrobial Studies
Abstract: Many herbal remedies have been employed in various medical systems for the treatment and management of different diseases. In this study, the purpose is to extract and isolate the alkaloid from leaf-galls of Ficus racemosa plant using suitable solvents by soxhlet extracter and performing the antimicrobial activities against different bacterial and fungal strains and in silico analysis on membrane proteins, outer membrane protein A tran-membrane domain (OmpA) and Feruloyl esterase …
Published in Research and Reviews : A Journal of Biotechnology · Vol. 3, Issue 2, 2013 · pp. 19–25 Read article
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Cytotoxicity of Titanium Dioxide Nanopaprticleson A Bacterial Population Isolated from Lakewater Sediments
Abstract: Studies suggest that approximately 4.6 million ton of pigmentary titanium dioxide (TiO2) is consumed annually worldwide. Owing to the increased industrial and consumer applications, there is growing concern regarding the possible risk associated with their environmental exposure. TiO2 dust, when inhaled, has been classified by the International Agency for Research on Cancer (IARC) as an IARC Group 2B carcinogen, meaning it is possibly carcinogenic to humans. TiO2 nanoparticles (NPs) can …
Published in Research and Reviews : A Journal of Biotechnology · Vol. 4, Issue 1, 2014 · pp. 31–35 Read article
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Isolation of Solid Waste Fungi
Abstract: The study area was Udupi, a temple town located in Karnataka state. Udupi is a land of celestial beauty and is inserted between the abundant mountains of the Western Ghats on the east and the immense calm Arabian Sea on the west. Temperature ranges from 30 to 350C in day time and is around 22–22°C±2°C during night. Udupi has a latitude of 13,3500 (1320'60.000"N), longitude of 74,7500 (7445'0.000"E) and Altitude …
Published in Research and Reviews : A Journal of Life Sciences · Vol. 5, Issue 1, 2015 · pp. 1–5 Read article
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Polyphasic Characterization and Comparative Genomics of a Newly Isolated Strain of Kocuria soli from Wild Ass Sanctuary, Gujarat, India
Abstract: From soil sample collected from Little Rann of Kutch-Wild Ass Wild Life Sanctuary, Gujarat a bacterial strain that is non-motile, cocci in shape, obligate aerobic, gram positive, catalase and oxidase positive was isolated and named as strain JC486. Strain JC486T was related to Kocuria soli M5W7-7T (99.7%) belonging to genus Kocuria according to sequence similarity and comparison data of 16S rRNA gene. The strain JC486T had a G+C content (DNA) …
Published in Journal of Water Pollution & Purification Research · Vol. 9, Issue 3, 2022 · pp. 16–22 Read article
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BIOCHEMICAL AND KINETICS STUDIES ON PROTEASE ENZYME ISOLATED FROM AYURVEDIC HERBS ALONG WITH THEIR APPLICATION STUDIES
Abstract: The objective of the present work was to conduct comprehensive biochemical and kinetic analysis of a protease enzyme extracted from two Ayurvedic herbs, namely Shankhpushpi (Convolvulus prostratus) and Mulethi (Glycyrrhiza glabra). The primary focus was to characterize various biochemical aspects of the enzyme, encompassing substrate specificity, optimal pH and temperature conditions, stability, and activation energy. Furthermore, crucial kinetic parameters such as Michaelis-Menten constant (Km) and maximum reaction velocity (Vmax) were …
Published in Research and Reviews : A Journal of Biotechnology · Vol. 13, Issue 3, 2023 · pp. 21–37 Read article
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Determining the Antagonistic Potential of Lactobacilli Isolated from Commercial Curd Samples against the E. coli
Abstract: Curd has always been an important source exhibiting a plethora of Lactobacilli. Lactobacilli are rod-shaped bacteria, capable of producing short-chain fatty acids, lactate, and acetate by carbohydrate fermentation process. A study was done to determine the antimicrobial activity of isolated and identified Lactobacilli bacteria from commercial curd samples against standard strains of E. coli. All the 10 samples were found have lactobacilli strains. The antagonistic activity of these strains was …
Published in Research & Reviews : Journal of Food Science & Technology · Vol. 9, Issue 2, 2020 · pp. 27–30 Read article
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First record of isolation of hemolymph and identification of hemocytes in a Cyclopoid Copepod
Abstract: Based on the literature review done it has been noted that it is the first report of observation and identification of hemocytes from Cyclopoid Copepod. The hemolymph sample was taken by a preliminary standard method and hemocytes were isolated and identified under light microcopy using eosin stain. The hemocytes observed were clarified based on the presence or absence and relative size of cytoplasmic granules. Three types of hemocytes were observed …
Published in Research and Reviews : A Journal of Life Sciences · Vol. 9, Issue 3, 2019 · pp. 42–45 Read article
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Evaluation of the Growth and Biomass Production of Eight Commercially Important Microalgae Species Isolated from Lake Tana, Ethiopia
Abstract: Microalgae are a diverse group of photosynthetic microorganisms that can be cultivated in a controlled condition for different economic and environmental applications. This study aimed to evaluate the growth and biomass productivity of some selected microalgae species isolated from Lake Tana (Ethiopia) in a laboratory suitable for mass cultivation. The study was conducted from February (2020) to January (2021). C-media was used for cultivation at an incubation condition of 2500 …
Published in Research and Reviews : A Journal of Life Sciences · Vol. 12, Issue 3, 2022 · pp. 13–21 Read article
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Chromosomal Aberration and Mutagenicity Toxicological Studies of Beta-Sitosterol Isolated from Ocimum gratissimum (Lamiaceae)
Abstract: Ocimum gratissimum is an important medicinal plant belonging to the Lamiaceae family. Ocimum gratissimum is a small erect or straggling tree or large, woody, climbing shrub found almost throughout India including Andaman and Nicobar Islands. Stem have been used in antifertility treatment in the indigenous system of medicine, and further clinical tests substantiated their efficacy. The root bark contains diketones, fatty matter, rubber, dulcitol, Beta-sitosterol, phlobatannin and glycosidal tannins. Stem …
Published in Research & Reviews: A Journal of Pharmacognosy · Vol. 4, Issue 2, 2017 · pp. 1–6 Read article
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Screening of Some Triterpenoids Isolated from Terminalia arjuna as Inhibitors of Angiotensin Converting Enzyme to Prevent Cardiovascular Diseases
Abstract: Natural products have been shown to present interesting biological and pharmacological activities and are used as chemotherapeutic agents. Plants have historically been used in treating cardiovascular diseases (CVD) and are recognized for their ability to produce secondary metabolites. Secondary metabolites obtained from different plants have been the starting material for designing different drugs. Many useful phytoconstituents have been isolated from Terminalia arjuna. Tannins and flavonoids are responsible for its anticancer …
Published in Research & Reviews: A Journal of Drug Design & Discovery · Vol. 1, Issue 3, 2014 · pp. 12–19 Read article
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Characterization of Endofungus Aspergillus nomius EF8-RSM Isolated from Aloe vera Western Ghats of Karnataka India
Abstract: The objective of the present research investigation was to reveal the versatile nature of endophytic Aspergillus nomius EF8-RSM. The fungus was purified from Aloe vera, Western Ghats of Karnataka, India and sequence was submitted at NCBI under the accession number MN871698. The fungus successfully produced Indole acetic acid at the rate of 49.21±6.1 μg/ml and phosphate solubilization was confirmed with solubilization index (1.63). The fungus showed production of siderophore, ammonia …
Published in Research and Reviews: A Journal of Pharmaceutical Science · Vol. 11, Issue 1, 2020 · pp. 22–30 Read article
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Synergistic and Antagonistic Effects of Azo-Schiff Base with Some Antibiotics on Gram-Positive Bacterial Isolates
Abstract: Antibacterial resistance has brought an urgent need to find a solution to the problem of antibacterial resistance and increase the effectiveness of antibiotics. Therefore, our current study was aimed to test the three concentrations viz., 0.5 mg/ml, 5 mg/ml and 50 mg/ml of Azo-Schiff base against Grampositive bacteria—Bacillus cereus, Micrococcus lutes, Staphylococcus aureus—to compare the effects of Schiff base alone or along with antibiotics on the viability of bacteria. Our …
Published in Research and Reviews: A Journal of Microbiology and Virology · Vol. 11, Issue 2, 2021 · pp. 25–31 Read article
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Performance Characteristics of Magnetorheological Elastomer Vibration Isolator
Abstract: Smart materials have ability to alter significantly its properties under external stimuli, such as stress, temperature, moisture, electric or magnetic fields. In today’s engineering era, various smart materials are being used to have controlled behavior in various applications such as automotive brakes, clutches, etc. Magneto Rheological Elastomer (MRE) is one of them, uprising with very huge potential. In this present work, preparation of MRE has been presented. Various MRE mold, …
Published in Journal of Automobile Engineering and Applications · Vol. 1, Issue 2, 2014 · pp. 1–4 Read article
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Performance Investigation on Boost Converter with Non-Isolated Coupled Inductor Using PSO-DFFP Controller with Stand-Alone PV System
Abstract: The stand-alone PV system with a Partial Swarm Optimization with Distinctive Feed Forward Propagation (PSO-DFFP) controller based Boost converter is designed with coupled inductor has been simulated. The steady state / dynamic performance of the proposed system have been analyzed using MATLAB simulation. The proposed controller has been developed to decrease ripple and improve the efficiency of the system. However, the linear controllers are influenced by the system parameters of …
Published in Journal of Control & Instrumentation · Vol. 13, Issue 1, 2022 · pp. 32–49 Read article
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ISOLATION OF CELLULOSIC NANOPARTICLES FROM COIR FIBRE FOR THE PREPARATION OF NATURAL RUBBER COMPOSITES
Abstract: Coir fibre and natural rubber are renewable and sustainable agro-resources that can be harnessed for the production of finished rubber articles on industrial scale. In this research, coir fibres extracted from coconut husk were subjected to alkaline-peroxide delignification. The delignified fibres were further transformed into coir fibre cellulosic nanoparticles (CFNP) with particle size distribution of 68–311 nm and a polydispersity index (PDI) of 0.371 through friction homogenization. These particles were …
Published in Journal of Polymer & Composites · Vol. 6, Issue 1, 2018 · pp. 9–16 Read article
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A Smart Grid System with Renewable Energy Sources for Isolated Operation
Abstract: This paper presents a smart grid which is utilizing power from a solar and wind energy conversion system and then it is given to a battery for the storage purpose. The smart system consists of a smart meter for calculating the energy which is utilized and it is regulated to the load which as advised. Using this system the power can be used for system which is operating without the …
Published in Recent Trends in Electronics Communication Systems · Vol. 6, Issue 2, 2019 · pp. 1–12 Read article
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Cell Membrane Immunogens of a Biofilm-producing Staphylococcus aureus Isolate Adjuvanted with Bacterial Chromosomal DNA Are Protective in a Mouse Mastitis Model
Abstract: Biofilm-forming staphylococci have been implicated in persistent bovine udder infections. Prophylactic potential of Staphylococcus aureus biofilm phenotype-associated immunogens in mouse models of systemic, but not intramammary infections has been demonstrated. In the present study, the authors observed protection in a murine model of intramammary infection by two subcutaneous injections at four-week interval of biofilm-forming S. aureus cell membrane antigens mixed with homologous chromosomal DNA as adjuvant, when challenged after eight …
Published in Research and Reviews : A Journal of Immunology · Vol. 3, Issue 3, 2013 · pp. 18–24 Read article
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Isolation of Atypical Mycobacteria from an Unusual Site: A Case Study
Abstract: Infections due to atypical mycobacteria have been reported after surgical procedures, liposuction, subcutaneous injections, etc. Here the authors report a case that was operated for umbilical hernia and presented with delayed wound healing, discomfort over site of incision and chronic serous discharge. The exudate from non-healing surgical wound was received in the microbiology laboratory. Ziehl-Neelsen’s stain of the exudate revealed presence of scanty acid fast bacilli which on culture on …
Published in Research and Reviews : A Journal of Immunology · Vol. 4, Issue 1, 2014 · pp. 13–16 Read article
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Cyto-toxicity of Silver Nanoparticles on A Bacterial Population Isolated From Lakewater
Abstract: Due to the increased industrial and consumer applications, there is growing concern regarding the possible risk associated with their environmental exposure. Silver nanoparticles are considered to be toxic both to environment and human beings after a permissible limit. The aim of the present study was to explore the difference in toxicity mechanism of Silver nanoparticles (NPs) at low concentrations (≤1 μg/mL), in a lake-water bacterial consortia containing Bacillus marisflavi and …
Published in Research and Reviews: A Journal of Toxicology · Vol. 4, Issue 1, 2014 · pp. 22–25 Read article
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Isolation of Micro-flora from Different Furniture Used In Hospital Set-Up
Abstract: Hospital-acquired infections are a major cause of worry for all hospitals worldwide and add to the problems of an already sick patient and also the personnel working. Hospitals are known to be high infection zones owing to the presence of large number of patients having some or other infectious etiologies or proneness to infection due to their immune-compromised state. Several studies have been done to evaluate the impact of these …
Published in Research and Reviews: A Journal of Toxicology · Vol. 13, Issue 2, 2023 · pp. 1–6 Read article