From soil sample collected from Little Rann of Kutch-Wild Ass Wild Life Sanctuary, Gujarat a bacterial strain that is non-motile, cocci in shape, obligate aerobic, gram positive, catalase and oxidase positive was isolated and named as strain JC486. Strain JC486T was related to Kocuria soli M5W7-7T (99.7%) belonging to genus Kocuria according to sequence similarity and comparison data of 16S rRNA gene. The strain JC486T had a G+C content (DNA) of 71 mol% and was found to have one amino phospholipid (APL), one aminolipid (AL), one phospholipid (PL), and five polar lipids which are unidentified, respiratory quinone MK-7, and the fatty acids (major) of strain JC486 include anteiso-C15:0 and anteiso-C17:0. Lysine and alanine are found in peptidoglycan of cell wall. Both strains have ANI and dDDH scores of 98 and 80.2, respectively. Based on physiological, chemotaxonomic and phylotaxogenomic studies, it is determined strain JC486 (= KCTC49118 = LMG 30604) is a strain of Kocuria soli