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61 articles for “mass spectrometer”
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Development and Validation of Liquid Chromatography-Tandem Mass Spectrometric Method for Assay of Zidovudine in Human UrineDevelopment and Validation of Liquid Chromatography-Tandem Mass Spectrometric Method for Assay of Zidovudine in Human Urine
Abstract: A sensitive and selective liquid chromatographic-tandem mass spectrometric (LC-MS-MS) method was developed to determine zidovudine (ZDV) in human urine. After solid-phase extraction with SPE cartridge, the urine sample was analyzed on a C18 column (Symmetry 3.5 µm; 75×4.6 mm i.d) interfaced with a triple quadrupole tandem mass spectrometer. Positive electrospray ionization was employed as the ionization source. The mobile phase consisted of water-acetonitrile (20:80, v/v).The method was linear over a …
Published in Research and Reviews: A Journal of Pharmaceutical Science · Vol. 5, Issue 3, 2014 · pp. 17–23 Read article
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Comparative Biochemical Study of Plant for Leaves and Bark of Ziziphius spina Christi L. Using Gas Chromatography Mass Spectrometry
Abstract: The aim is Comparative Pharm-Chemical Study for Leaves and Bark of Ziziphius spina Christi L. Using Gas Chromatography Mass Spectrometry. The Biochemical study dealt with the chemical content of the leaves and bark of the stem of the studied Ziziphius spina Christi.L, as the medicinal-chemical compounds were diagnosed using Gas Chromatography Mass Spectrometry (GC/Mass) by comparing the chemical content. appearance of the repetition of some chemical compound Hexen-2-one,3-methyl-, Oxalic acid, …
Published in Research and Reviews : A Journal of Biotechnology · Vol. 12, Issue 3, 2022 · pp. 38–46 Read article
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Mass Spectrometry–Based Phosphoproteomic Markers to Predict Kinase Inhibitor Response in Solid Tumors
Abstract: Mass spectrometry-based phosphoproteomics has emerged as a powerful tool for predicting kinase inhibitor responses in solid tumors, offering direct functional insights into signaling pathways that surpass traditional genomic profiling by capturing dynamic kinase activities and adaptive resistance mechanisms. Technological breakthroughs, including data- independent acquisition (DIA), trapped ion mobility spectrometry (timsTOF), and efficient enrichment methods like TiO2 or IMAC, now enable comprehensive profiling of over 40,000 phosphorylation sites from limited clinical …
Published in International Journal of Cell Biology and Cellular Functions · Vol. 4, Issue 2, 2026 Read article
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Development and Validation of Liquid Chromatography- Tandem Mass Spectrometric Method for Assay of Zidovudine in Human Urine
Abstract: A sensitive and selective liquid chromatographic-tandem mass spectrometric (LC-MSMS)method was developed to determine zidovudine (ZDV) in human urine. After solidphaseextraction with SPE cartridge, the urine sample was analyzed on a C18 column(Symmetry 3.5 μm; 75×4.6 mm i.d) interfaced with a triple quadrupole tandem massspectrometer. Positive electrospray ionization was employed as the ionization source. Themobile phase consisted of water-acetonitrile (20:80, v/v).The method was linear over aconcentration range of 10–1000 ng mL-1. …
Published in Research and Reviews: A Journal of Pharmaceutical Science · Vol. 5, Issue 3, 2014 · pp. 17–23 Read article
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Determination of Gatifloxacin in Human Urine by Liquid Chromatography-Tandem Mass Spectrometry
Abstract: Gatifloxacin (GTF) is an antibiotic of the fourth-generation fluoroquinolone family that inhibits the bacterial enzymes DNA gyrase and topoisomerase IV. A reliable, sensitive and selective isocratic reversed phase liquid chromatographic-tandem mass spectrometric (LC-MS-MS) method was developed to determine gatifloxacin (GTF) in human urine. GTF, in urine after solid-phase extraction with SPE cartridge, was analyzed on a C18 column (Symmetric 3.5 μm; 75×4.6 mm i.d) interfaced with a triple quadrupole tandem …
Published in Research and Reviews: A Journal of Pharmaceutical Science · Vol. 6, Issue 1, 2015 · pp. 1–6 Read article
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Gas chromatography mass spectrometry and Fourier transform infrared spectral analyses of Albizia lebbeck (L.) Benth. pod methanol extract
Abstract: This study was aimed at the elucidation of the bioactive compounds present in methanol extracts of Albizia lebbeck (L.) Benth. Pod using Gas chromatography–mass spectrometry (GC-MS) and Fourier transform infrared spectroscopic (FTIR) techniques. The GC-MS mass spectra of the identified compounds were compared with those of the National Institute of Standards and Technology (NIST) database library. The results confirmed the presence of five compounds including Pentasiloxane, 1,1,3,3,5,5,7,7,9,9-decamethyl-, Heptasiloxane, hexadecamethyl-, Methyl …
Published in Journal of Modern Chemistry & Chemical Technology · Vol. 10, Issue 3, 2019 · pp. 34–40 Read article
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Application of an Online Chromium Reduction System for D/H Analysis of Water Samples with Large Isotopic Variations Using Conventional Mass Spectrometer
Abstract: We present an online chromium (Cr) reduction system for measuring the D/H ratio of water samples exhibiting significant isotopic contrast, where the memory effect cannot be overlooked. Memory compensation is incorporated into the analysis procedure to mitigate this effect, allowing the utilization of injection results that would otherwise be discarded due to the memory effect. This approach enables a faster analysis and an increased sample throughput. Additionally, crucial aspects of …
Published in Journal of Instrumentation Technology & Innovations · Vol. 15, Issue 1, 2025 · pp. 7–12 Read article
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Structural Characterization of Flavonoids Using Mass Spectrometry
Abstract: The objective of this paper is to review the fragmentation pattern of commonly known flavonoids in mass spectrometry (MS) along with brief examples. In the current review, literature search was conducted using Elsevier, Science Direct, Springer Link (Springer), PubMed and Google Scholar. The search included the keywords “flavonoids”, “C-, O-glycoside flavonoids”, “aglycan flavonoids‟‟ cross-referenced with the keywords “characterization”, “elucidation”, ”fragmentation”. Several authors reported that the main fragmentation paths of flavonoids …
Published in Research and Reviews: A Journal of Pharmacology · Vol. 6, Issue 3, 2016 · pp. 29–47 Read article
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Analysis for Respiratory Transmittable Diseases via Mass Spectrometry
Abstract: Microbial identification in clinical diagnostic laboratories has mainly relied on conventional phenotypic and gene sequencing identification techniques. The development of matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) devices has revolutionized the repetitive identification of microorganisms in clinical microbiology laboratories by introducing an easy, rapid, high throughput, low-cost, and effective identification technique. This technology has been modified to the restraint of clinical diagnostic laboratories and has likely to replace …
Published in Research and Reviews: A Journal of Microbiology and Virology · Vol. 7, Issue 1, 2017 · pp. 26–30 Read article
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Bioactive Compounds in Ethanolic Extract of Strychnos innocua Root Using Gas Chromatography and Mass Spectrometry (GC-MS)
Abstract: Medicinal plants are of great relevance with endless pharmaceutical and therapeutic properties. They are source of wide array of secondary metabolites or bioactive compounds/phytochemicals (phenols, alkaloids, flavonoids, terpenoids, tannins, steroids and saponins) which can discharge numerous biological functions (antimicrobial, antioxidant, antiviral, antifungal, anti-fibrotic, immune-modulatory, cytotoxic, antipyretic, antitumor, antihelminthic, antiprotozoal, antibacterial, and so on). The use of gas chromatography and mass spectrometry analysis in identifying the bioactive compounds in the ethanolic …
Published in International Journal of Biochemistry and Biomolecule Research · Vol. 1, Issue 1, 2023 · pp. 9–14 Read article
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Liquid Chromatography Mass Spectrometer (LC/MS) Profile Revealed Flavonoids with Antioxidant Potential in Butanol Fraction of Gymnema Sylvestre Leaf Extract
Abstract: Gymnema sylvestre (Asclepiadaceae) is widely used in the treatment of several diseases associated with oxidative stress. This study aimed to identify Flavonoids with antioxidant potentials in Butanol fraction of Gymnema sylvestre leaf extract. In vitro antioxidant activity was studied using DPPH and Ferric Reducing Antioxidant Power (FRAP). The results revealed higher radical scavenging activity of 86.53±0.86 in DPPH compared to the control Ascorbic acid with 84.71±0.61 and also similarly in …
Published in Research & Reviews: A Journal of Pharmacognosy · Vol. 9, Issue 1, 2022 · pp. 26–39 Read article
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Comprehensive Analysis of MRSA Peptides Via Maldi
Abstract: The present study employed Matrix-Assisted Laser Desorption/Ionization Time-of-Flight mass spectrometry to analyze methicillin-resistant Staphylococcus aureus peptides, focusing on various parameters associated with mass-to-charge (m/z) values. Through systematic data collection and analysis, including time, intensity, signal-to-noise ratios, quality factors, resolutions, areas under the peaks, relative intensities, full widths at half maximum, Chi-squared values, and background peaks, comprehensive insights into the spectral characteristics of methicillin-resistant Staphylococcus aureus peptides were obtained. Methicillin-resistant Staphylococcus …
Published in Research and Reviews: A Journal of Pharmaceutical Science · Vol. 15, Issue 2, 2024 · pp. 98–101 Read article
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Single-Cell Proteomics approach for the development of Climate-resilient crops
Abstract: Over a last few years Single cell RNA sequencing technique has been growing at a faster pace but still the technique is unable to reveal the complexity and diversity of a cell. This void in the knowledge has led scientists to go for Single cell Proteomics. As proteins are the major work-horses of a cell they could better explain the unsolved cellular mysteries. Further the advancement in the mass spectrometry …
Published in Research and Reviews : A Journal of Biotechnology · Vol. 11, Issue 3, 2021 · pp. 5–9 Read article
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Predicting Rate Equation for the Synthesis of Octadec-9-Enoic Acid Methyl Esters by the Methanolysis of Pongamia Pinnata Triglycerides Using Sono Reactor
Abstract: In the present work, octadec-9-enoic acid methyl esters are synthesized from pongamia pinnata oil using methanolysis in sono reactor. The rate equation is predicted for the methanolysis reaction. The produced methyl esters from pongamia pinnata oil confirmed to ASTM D6751 standards. Fourier Transform Infrared Analysis and Gas Chromatography Mass Spectrometry were used to characterise the methyl esters. The rate is dependent greatly on methanol concentration, and then on catalyst concentration …
Published in Journal of Polymer & Composites · Vol. 9, Issue 3, 2021 · pp. 45–55 Read article
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Innovative Mechanism for Recycling Nano Biomaterials for Technological Eases
Abstract: In this paper, the nanobiomaterials and their suspected biotoxic effect are presented. The mechanism used to minimize such severe problems is recycling nanowastes and utilizing for an alternative purpose. An innovative mechanism established in order to renew the outdated nanobiomaterials is recycling. Hence, the need to reduce cost, improve quality, utilize and clean surrounding to ensure the environmental safety is studied. Therefore, two-way method is developed in order to recycle …
Published in Journal of Nanoscience, NanoEngineering & Applications · Vol. 12, Issue 2, 2022 · pp. 6–15 Read article
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Comparative Proteomics: From Cell Lines to Clinical Samples
Abstract: Comparative proteomics is a powerful tool for understanding the molecular differences between various biological samples. It entails identifying and measuring proteins in complex biological samples to assess their abundance, modifications, and interactions under various conditions. This approach plays a crucial role in advancing biomedical research, especially in disease understanding, biomarker discovery, and therapeutic development. While cell lines are widely used for proteomic studies due to their controlled environments and reproducibility, …
Published in Research and Reviews : Journal of Computational Biology · Vol. 13, Issue 3, 2024 · pp. 30–34 Read article
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Chemical Profile and Toxicity of Fresh and Dry leaves of the Essential Oils of Vetiveria zizanioides (L.) Nash. From Southwest Nigeria
Abstract: The fresh and dry leaves of Vetiveria zizanioides (L.) Nash (Gramineae) plant was collected, air dried and authenticated at the University of Ibadan Herbarium with voucher number: UIH-23237. The plant's dried, fresh leaves were hydro distilled in a Clevenger device to extract the essential oils. The oils were characterized by Gas Chromatography-Mass Spectrometry (GC – MS) while the toxicity of the essential oils was tested using brine shrimps’ lethality assay. …
Published in Recent Trends in Cosmetics · Vol. 1, Issue 1, 2024 · pp. 44–49 Read article
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Clinical metabolomics: Useful insights, perspectives, and challenges.
Abstract: Metabolomics provides a comprehensive snapshot of smallmolecule intermediates and end products of cellular processes and thereby links genotype, environment, and phenotype in human disease . Over the last decade, advances in liquid chromatography–mass spectrometry (LC–MS) and nuclear magnetic resonance (NMR) spectroscopy have enabled high-throughput profiling of thousands of metabolites from microliter volumes of clinical samples . Clinical metabolomics is now emerging as a tool for disease diagnosis, risk prediction, therapeutic …
Published in Emerging Trends in Metabolites · Vol. 3, Issue 1, 2026 · pp. 29–37 Read article
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Engineering Anti-Tau Monoclonal Antibodies for Alzheimer’s Disease and Parkinsonian Tauopathies: A Biotechnological Perspective
Abstract: Neurodegenerative disorders such as Alzheimer’s disease and Parkinson’s disease are characterized by progressive neuronal loss associated with abnormal protein aggregation. Among these, tauopathies are defined by the accumulation of hyperphosphorylated tau protein, which plays a central role in disease progression. Advances in biotechnology have enabled the development of anti-tau monoclonal antibodies (mAbs) as promising therapeutic agents aimed at neutralizing pathological tau species and inhibiting their propagation. This review provides a …
Published in Research and Reviews : A Journal of Biotechnology · Vol. 16, Issue 1, 2026 · pp. 52–58 Read article
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Characterization of a Broadly Specific LPS-Neutralizing Single Domain Antibody
Abstract: Gram negative endotoxaemia or sepsis remains a lethal challenge to humans and animals, globally. Single domain antibodies (dAbs) have emerged as therapeutic biologics for challenging antigens such as lipopolysachharide (LPS). The present study characterized a broadly specific LPS-neutralising dAb produced from phage display library from LPS-immunized Indian desert camel, for its sequence validation with mass spectrometry. Also, the dAb clone 26 was subjected to co-crystallisation with LPS, extracted from E. …
Published in Research and Reviews : A Journal of Immunology · Vol. 7, Issue 1, 2017 · pp. 1–7 Read article