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9 articles for “SDS-gel”
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Copper sulphate enhanced synthesis of azurin from Pseudomonas aeruginosa MTCC 2453
Abstract: The secondary metabolites from microorganisms play a vital role in developing new chemotherapeutics. Azurin a blue copper protein have redox properties plays a role in electron transfer as donors. Azurin reported as a potential anticancer protein against breast cancer cell lines which evoked biomedical researchers to develop an alternate method for enhanced production of azurin. Early researchers revealed the synthesis of blue copper protein azurin from different microbial sources, especially …
Published in Research and Reviews: A Journal of Microbiology and Virology · Vol. 1, Issue 1, 2011 Read article
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Development of Anti IgG Polyclonal Antibody Against Canine IgG and Their Roles in Diagnostic Purposes
Abstract: IgG plays an important part in immunological studies and also in living bodies for providing secondary immune response which triggers as passive immunization against harmful pathogens in dogs. Purpose: The aim of this investigation is to separate, identify, and refine immunoglobulin G from canine serum. Methods: Ammonium sulphate precipitation was used to separate the dog's serum, which was then concentrated over night in a dialysis bag inside of a refrigerator. …
Published in International Journal of Vaccines · Vol. 1, Issue 2, 2024 · pp. 01–06 Read article
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Differentiation of Hybrid (COTH2) and Wild (CO3) Varieties of Tomato (Lycopersicon esculentum) Using Protein and Peroxidase Isozyme Profile
Abstract: The protein profile by SDS-PAGE and the native PAGE profile of the isoenzyme peroxidase of a hybrid tomato plant (Lycopersicon esculentum) variety (COTH2) and wild-type plants (CO3) were compared in order to adopt them as markers to differentiate them. The hybrid plant, which were resistant to leaf curl disease, was found to have higher protein content compared to its wild variety in the leaf extracts of the plants. Presence of …
Published in Research and Reviews : A Journal of Biotechnology · Vol. 1, Issue 2, 2011 Read article
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Cloning and Expression of LPS-Binding Single Domain Antibody Clones Selected from Phage Display Library of Indian Desert Camel
Abstract: Despite the absence of light chains in their structure, camelid and shark “heavy chain antibodies” (HCAbs) variable domains (designated as VHH) are able to make functional paratopes. The authors have selected lipopolysaccharide (LPS)-binder “single-domain antibodies” (dAb) clones from the previously constructed phage display library of (LPS)-immunized Indian desert camel. This study was undertaken to make available large amounts of some LPS-binder dAb clones by using a suitable expression vector-host system …
Published in Research and Reviews : A Journal of Immunology · Vol. 4, Issue 1, 2014 · pp. 1–7 Read article
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Taxonomic Assessment of Thermostable Amylase Producer from the Unkeshwar Hot Spring, Nanded
Abstract: Total 42 thermophiles were isolated from the Unkeshwar hot spring of Maharashtra, India. Of these 23 thermostable amylase producers were identified as Bacillus alvei APP1, Bacillus brevis APP2, Bacillus sterothermophilus APP3, Bacillus subtilis APP4, Bacillus farraginis APP5, Bacillus firmus APP6, Bacillus licheniformis APP7, Bacillus megaterium APP8, Lysinibacillus sphaericus APP10, Paenibacillus alvei APP11, Bacillus simplex APP12, Staphylococcus cohnii APP15, Streptococcus thermophilus APP16, Bacillus cereus APP18, Bacillus atrophaeus APP19, Bacillus lentus APP24, …
Published in Research and Reviews: A Journal of Microbiology and Virology · Vol. 9, Issue 1, 2019 · pp. 113–126 Read article
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POTENTIAL BIOPRESERVATIVE EFFECT OF ANTIMICROBIAL PEPTIDES OF SEEDS OF Vigna unguiculata (Cow Pea) ON Hibiscus sabdariffa L. (Roselle) Calyces (Zobo) DRINK
Abstract: Natural and efficient food preservatives are in greater demand because of growing concerns about the safety and quality of processed beverages. Antimicrobial peptides have emerged as promising natural substitutes for synthetic preservatives, boasting advantages like wide-ranging activity and reduced toxicity. In this research, we investigate the inhibitory effects of these peptides against common spoilage microorganisms in 'Zobo' drink. Peptides were extracted and purified from germinated Vigna unguiculata) seeds harvested on …
Published in Research & Reviews : Journal of Food Science & Technology · Vol. 14, Issue 1, 2025 · pp. 26–41 Read article
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Gelatin-EiO2 (Ei = Si/Ti/Zr) Based Mesoporous Nano-hybrids: Synthesis and Characterization
Abstract: The hybrid materials are mostly prepared by combining the organic or bio-organic part to the inorganic building blocks and are used in many applications. Gelatin as one of the main component of hybrid materials is an attractive option. It is biodegradable; biocompatible; non-toxic; bio-adhesive and contains many active groups such as –NH2, –COOH, in the biopolymer backbone makes these hybrid materials important in various technological applications. The synthesis of a …
Published in Journal of Materials & Metallurgical Engineering · Vol. 14, Issue 3, 2024 · pp. 30–58 Read article
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Phenetic Analysis of Solanum melongena Accessions to Explore the Genetic Diversity by SDS-PAGE
Abstract: The present experimental work was conducted for the phenetic analysis among 40 accessions of Solanum melongena from different geographical areas of Pakistan. The interspecific variation was found among the accessions of S. melongena. The analysis of seed storage was done by SDS-PAGE to check their relationship. Electrophoretic data was documented by gel documentation system (Bio-Rad, USA), and analyzed by using quantity 1-D analysis software and dendogram constructed with 100% tolerance …
Published in Research and Reviews: A Journal of Pharmacology · Vol. 2, Issue 2, 2012 Read article
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Application of pET28a Vector for the Over Expression of Recombinant Human Tim23 Protein in E. coli and its Validation by Immunoblotting
Abstract: This study aimed to produce recombinant human Tim23 protein by molecular cloning, expression, and purification. Amplification of human TIM23 was carried out by polymerase chain reaction (PCR) and cloned into DH5α cells. The pET28a-human TIM23 was transformed into BL21DE3 and expressed the gene by IPTG induction. Recombinant pure human Tim23 antigen was prepared, and injected into rabbits and polyclonal antibodies were raised, which was confirmed by western blotting. The transformed …
Published in Research and Reviews : A Journal of Life Sciences · Vol. 14, Issue 1, 2024 · pp. 32–44 Read article