Taxonomic Assessment of Thermostable Amylase Producer from the Unkeshwar Hot Spring, Nanded

Anupama Prabhakarrao Pathak; Mukundraj Govindrao Rathod

Abstract

Total 42 thermophiles were isolated from the Unkeshwar hot spring of Maharashtra, India. Of these 23 thermostable amylase producers were identified as Bacillus alvei APP1, Bacillus brevis APP2, Bacillus sterothermophilus APP3, Bacillus subtilis APP4, Bacillus farraginis APP5, Bacillus firmus APP6, Bacillus licheniformis APP7, Bacillus megaterium APP8, Lysinibacillus sphaericus APP10, Paenibacillus alvei APP11, Bacillus simplex APP12, Staphylococcus cohnii APP15, Streptococcus thermophilus APP16, Bacillus cereus APP18, Bacillus atrophaeus APP19, Bacillus lentus APP24, Bacillus stearothermophilus APP27, Bacillus larvae APP35, Bacillus subtilis APP36, Bacillus aminovorans APP38, Microbacterium sp. APP39, Microbacterium sp. APP40 and Microbacterium sp. APP41. Production of thermostable amylases was carried out at 50°C temperature using amylase production media. Partially purified amylases from these isolates exhibited specific activity in the range of 80.12–200.88 U/mg. Maximum 90% yield of amylase was recorded from Bacillus brevis APP2. The molecular weights of partially purified amylases from B.alvei APP1, B.brevis APP2, B.sterothermophilus APP3, B.subtilis APP4, B.farraginis APP5 and B. firmus APP6 were recorded in the range of 45–65 kDa by sodium dodecyl sulphate (SDS) polyacrylamide gel electrophoresis (PAGE; 12%). The partially purified amylases from the Unkeshwar hot spring isolates have showed optimum catalytic activity at the range of 15–25 mg/ml starch concentration and remarkable stability at a wide range of temperature from 35 to 75°C. Keywords: Thermostable amylase, Unkeshwar hot spring, SDS-PAGE, Km, VmaxCite this ArticlePathak AP, Rathod MG. Taxonomic assessment of thermostable amylase producer from the Unkeshwar hot spring Nanded. Research & Reviews: A Journal of Microbiology and Virology. 2019; 9(1): 113–126p.