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25 articles for “PCR amplification”
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An Improved Method for the Isolation of High Quality Genomic DNA from Axenic Cultures of Filamentous Blue-green algae (Cyanobacteria), Nostoc and Anabaena
Abstract: Blue-green algae are one of the important organisms having different ecological and economic important properties and are among one of the least explored one. The major difficulty is met with their exact identity; this could be tackled to an extent with the use of molecular techniques. Isolation of high quality DNA is essential for molecular biology applications. In the present study an efficient protocol for the extraction of genomic DNA …
Published in Research and Reviews : A Journal of Life Sciences · Vol. 9, Issue 2, 2019 · pp. 32–38 Read article
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Bioinformatics-based Characterization of Stress Tolerance Spermidine Synthase from Zea mays
Abstract: The response to sensing environmental stress by plants is very important and can be short term by the synthesis of some protecting molecules against stress or long term by expressing phenotypic plasticity with alteration of developmental programme. The ability to respond to abiotic stress represents the main constraint on maize production. The spermidine synthase genes (Zmspds1 and Zmspds2) in Zea mays genome were identified by the PCR amplification and expression …
Published in Research and Reviews : A Journal of Life Sciences · Vol. 3, Issue 2, 2013 · pp. 26–33 Read article
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Preliminary In Silico Analysis of Choline Monooxygenase and Betaine Aldehyde Dehydrogenase from Suaeda monoica Forssk. and its Expression in E. coli.
Abstract: AbstractIn a tropical country like India, the yield of crop plants is majorly affected by soil salinity and drought. One of the strategies to address this problem is by introducing genes responsible for salinity/drought tolerance in crop plants. Such genes could be identified and isolated from mangrove plants, as they are known to have evolved certain physiological mechanisms to augment saline environments. One such mechanism is the accumulation of Glycine …
Published in Research and Reviews : Journal of Computational Biology · Vol. 9, Issue 1, 2020 · pp. 10–39 Read article
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Pathogenesis, Treatment and Molecular Mechanisms for Development of Multidrug-resistant (MDR) TB as well as Identification of MDR Mycobacterium Tuberculosis
Abstract: Normal 0 false false false EN-US X-NONE X-NONE Tuberculosis (TB) is a medical, social, and economic disaster of huge magnitude in the world. Prevalence of TB has increased due to poverty, population growth, migration and a high number of TB cases in HIV positive individuals. High prevalence rate of M. tuberculosis strains with multidrug resistance (MDR) is a serious problem as global emergency. Strains of M. tuberculosis resistant to both …
Published in Research and Reviews: A Journal of Microbiology and Virology · Vol. 3, Issue 1, 2013 · pp. 16–38 Read article
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A Duplex PCR for Simultaneous Detection of Brucella and Bovine Herpesvirus – Genome in Clinical Samples
Abstract: Normal 0 false false false EN-US X-NONE X-NONE MicrosoftInternetExplorer4 A disease-free germplasm is a prerequisite for getting healthy progeny. Many diseases are transmitted through semen either by natural service or artificial insemination causing reproduction failure and economic losses. The intermittent excretion of Bovine Herpesvirus-1 (BoHV-1) and Brucella in semen and their transmission through artificial insemination has been recorded. PCR has become an invaluable tool because of speed and simplicity with …
Published in Research and Reviews: A Journal of Microbiology and Virology · Vol. 1, Issue 2, 2011 · pp. 1–7 Read article
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Identification of Bacterial Strain from Waste Decay Material Using 16s rRNA Ribotyping
Abstract: Ribotyping is a classical technique in molecular studies where conserved and unique DNA sequences, more specifically 16s RNA, are investigated to differentiate among strains of microorganisms. This method was utilized in the current study to identify the strains of bacteria present in waste decaying matter. Waste material was collected from Indian Institute of Technology (IIT), Delhi campus, Hauz Khas, New Delhi, and cultured for bacterial growth. Purified bacterial cultures were …
Published in Research and Reviews : A Journal of Life Sciences · Vol. 6, Issue 3, 2016 · pp. 1–4 Read article
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Cloning and Expression of LPS-Binding Single Domain Antibody Clones Selected from Phage Display Library of Indian Desert Camel
Abstract: Despite the absence of light chains in their structure, camelid and shark “heavy chain antibodies” (HCAbs) variable domains (designated as VHH) are able to make functional paratopes. The authors have selected lipopolysaccharide (LPS)-binder “single-domain antibodies” (dAb) clones from the previously constructed phage display library of (LPS)-immunized Indian desert camel. This study was undertaken to make available large amounts of some LPS-binder dAb clones by using a suitable expression vector-host system …
Published in Research and Reviews : A Journal of Immunology · Vol. 4, Issue 1, 2014 · pp. 1–7 Read article
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Comparative Efficacy of Hybrid Capture 2 and Real-Time PCR in Detecting High-Risk HPV Genotypes for Cervical Cancer Screening
Abstract: Human papillomavirus (HPV) infection is a leading cause of cervical cancer, making early detection critical for effective prevention and treatment. Among the diagnostic methods available, Hybrid Capture 2 (HC2) and Real-Time Polymerase Chain Reaction (PCR) are widely used for detecting high-risk HPV genotypes, particularly HPV 16 and 18, which account for the majority of cervical cancer cases. This review aims to compare the efficacy of HC2 and Real-Time PCR in …
Published in International Journal of Genetic Modifications and Recombinations · Vol. 2, Issue 2, 2024 · pp. 13–17 Read article
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Semi-quantitative Event Specific Detection of Bt Maize (Event MON 810) using Real-Time PCR with SYBR Green Ι Chemistry
Abstract: The objective is this work is to do semi-quantitative analysis for Bt maize (event MON 810) using conventional Polymerase Chain Reaction (PCR) and Real Time PCR (SYBR green chemistry) from the fresh maize samples chosen arbitrarily from various locations. The protocol for extraction of good quality genomic Deoxyribonucleic acid (DNA) was standardized without the use of Liquid nitrogen. The DNA used for further studies were obtained by using standardized protocol. …
Published in Research and Reviews : A Journal of Biotechnology · Vol. 2, Issue 3, 2012 · pp. 25–34 Read article
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Role of MSG (Monosodium Glutamate) in DNA Breaks by Cellular Method: A review article
Abstract: - Monosodium glutamate (MSG) is a generally used food additive to enhance flavors. It has been found that though is one of the most common flavoring agents used worldwide, MSG’s accumulation in the body can cause genomic damages. These genomic damages can be identified through various methods such as Random Amplified Polymorphic DNA polymerase chain reaction (RAPD - PCR). Some herbal extracts have medicinal properties that can initiate DNA repair …
Published in Research & Reviews: A Journal of Bioinformatics · Vol. 10, Issue 2, 2023 · pp. 1–5 Read article
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Amplification of 16s rRNA Gene of Brucella melitensis Causing Brucellosis Isolated from the Aborted Fetes of Goat
Abstract: ABSTRACTBrucella melitensis is zoonosis caused by facultative intracellular bacteria of genus Brucella, which are widely distributed in both human and animals, especially in developing world. The diagnosis of goat brucellosis requires isolation of the bacteria from stomach contain aborted fetes of goat. However cultural sampling sensitivity often low, depending on the disease stage, Brucella species, culture medium, quantity of circulating bacteria and blood culture technique employed. The development of PCR …
Published in Research and Reviews : Journal of Veterinary Science and Technology · Vol. 9, Issue 1, 2020 · pp. 8–12 Read article
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Evaluation of Nucleic Acid Amplification Method in Diagnosing Enteric Fever in Children
Abstract: The comparison of three detection methods (Widal test, Typhi dot and Nested PCR) for diagnosis of typhoid fever was done in laboratory where gold standard was either Widal positive or Typhi dot positive or both positive. A nested polymerase chain reaction (PCR) specific for Salmonella enteric serovar typhi was used for the detection of the pathogen in blood samples. Total 52 patients with clinical suspicion of typhoid fever were recruited …
Published in Research and Reviews: A Journal of Microbiology and Virology · Vol. 3, Issue 1, 2013 · pp. 9–15 Read article
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A Comprehensive Review on Monkeypox Infection
Abstract: Monkeypox represents a zoonotic viral affliction instigated by the monkeypox virus, which is classified within the Poxviridae family and is distinct from the virus that causes chickenpox. Initially recognized in 1958, this infection has recently attracted international scrutiny due to its escalating incidence beyond traditional endemic territories. This review delivers an exhaustive examination of monkeypox, encompassing its epidemiology, viral morphology, modes of transmission, pathogenesis, clinical presentations, and therapeutic interventions. The …
Published in Recent Trends in Infectious Diseases · Vol. 2, Issue 1, 2025 · pp. 23–32 Read article
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In vitro Antiviral Chemical Treatment to BBTV-Infected Banana Cultures for Production of Virus-Free Plants
Abstract: Obtaining healthy plants from infected germplasm banana stocks is a major problem. Banana bunchy top virus (BBTV) is the most destructive pathogen for the banana crop. Production of virus-free plants is crucial for cultivation, germplasm conservation and their exchanges. In vitro shoot meristem culture is the most common method for producing disease-free planting material. However, BBTV can multiply with in vitro cultures too. Therefore, for eliminating BBTV, the shoot-tip culture …
Published in Research and Reviews : A Journal of Life Sciences · Vol. 9, Issue 3, 2019 · pp. 28–33 Read article
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Application of pET28a Vector for the Over Expression of Recombinant Human Tim23 Protein in E. coli and its Validation by Immunoblotting
Abstract: This study aimed to produce recombinant human Tim23 protein by molecular cloning, expression, and purification. Amplification of human TIM23 was carried out by polymerase chain reaction (PCR) and cloned into DH5α cells. The pET28a-human TIM23 was transformed into BL21DE3 and expressed the gene by IPTG induction. Recombinant pure human Tim23 antigen was prepared, and injected into rabbits and polyclonal antibodies were raised, which was confirmed by western blotting. The transformed …
Published in Research and Reviews : A Journal of Life Sciences · Vol. 14, Issue 1, 2024 · pp. 32–44 Read article
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Association of Vitamin D Binding Protein BP rs2282679 Gene Polymorphism and Serum Levels of Vitamin D in Patients with Vitiligo
Abstract: For vitamin D to have an impact, the vitamin D receptor (VDR) must be expressed and activated in the nucleus. The VDR has several known genetic variants. Biological impacts can be caused by changes in DNA sequences known as "polymorphisms" that are common in the population. Vitiligo is a disease that causes loss of skin color in patches, and it is a chronic (long-lasting) autoimmune disorder loss of color. The …
Published in Research and Reviews : A Journal of Biotechnology · Vol. 14, Issue 2, 2024 · pp. 45–52 Read article
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CRISPR, Recombinant DNA, and LAMP-Based Platforms for Monkeypox: Emerging Tools for Diagnosis and Therapeutic Development
Abstract: Monkeypox, a zoonotic viral infection caused by the Monkeypox virus of the Orthopoxvirus genus, has recurred as a worldwide public health concern after recent outbreaks outside of its classical endemic areas in Central and West Africa. The resurgence has demanded a thorough reassessment of its epidemiology, mode of transmission, and clinical presentation. In light of these challenges, recombinant DNA technology (rDNA) has emerged as a candidate for developing vaccines, diagnostics, …
Published in International Journal of Virus Studies · Vol. 3, Issue 1, 2026 · pp. 7–28 Read article
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Detection of Listeria monocytogenes in Milk and Ice Cream by Polymerase Chain Reaction
Abstract: Abstract The present study was undertaken to standardize PCR assay for detection of L. monocytogenes and Listeriolysin O from livestock foods and compare its efficacy with conventional cultural methods. A set of primer derived from iap gene and other set derived from hlyA gene were used for detection of L. monocytogenes and Listeriolysin O in the PCR assay. The PCR assay was initially standardized by optimizing the concentration of the …
Published in Research and Reviews: A Journal of Microbiology and Virology · Vol. 5, Issue 1, 2015 · pp. 24–35 Read article
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Existence of Integron I and II Genes from Clinical Isolate Staphylococcus aureus and Their Role in Drug Resistance
Abstract: An integron is a gene capture and dissemination system. They have been identified on both genomic and mobile genetic elements which often contain one or more genes that encode antibiotic resistance. They are commonly associated with bacterial genera in the family of Enterobacteriaceae. Currently, there have been recent reports showing the presence of integrons in Staphylococcus species. The aim of the present study was to assess the existanxe of Class …
Published in Research and Reviews: A Journal of Microbiology and Virology · Vol. 4, Issue 3, 2014 · pp. 18–24 Read article
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The Detection of Virulence Associated Genes of E. coli O157:H7 by Multiplex PCR
Abstract: An easy and quick system is desirable to detect toxin-producing strains in view of the importance E. coli serotype O157:H7 in human diseases. We report standardization of a multiplex polymerase chain reaction (mPCR) test for simultaneous identification of four significant genes related with the life form E. coli O157:H7 viz, stx 1, stx 2, eae A, hly. An alongside an internal amplification control (IAC). The mPCR technique created in the …
Published in Research & Reviews: A Journal of Bioinformatics · Vol. 6, Issue 3, 2019 · pp. 21–25 Read article