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Research and Reviews : A Journal of Biotechnology Original Research

Association of Vitamin D Binding Protein BP rs2282679 Gene Polymorphism and Serum Levels of Vitamin D in Patients with Vitiligo

  1. Ali S. Shakir Department of Clinical Immunology, College of Dentistry, University of Al-Qadisiyah
  2. Alaa T. Hamza Department of Biochemistry, College of Dentistry, University of Al-Qadisiyah

Abstract

For vitamin D to have an impact, the vitamin D receptor (VDR) must be expressed and activated in the nucleus. The VDR has several known genetic variants. Biological impacts can be caused by changes in DNA sequences known as "polymorphisms" that are common in the population. Vitiligo is a disease that causes loss of skin color in patches, and it is a chronic (long-lasting) autoimmune disorder loss of color. The skin turns a milky white color because the skin cells that create pigment, called melanocytes, are attacked, and destroyed. For comparison, the study included 80 samples total; 40 of them came from vitiligo sufferers (male and female) and 40 from healthy individuals. Under aseptic conditions, five milliliters of blood were collected by puncturing a vein with disposable syringes. The 2 ml of each sample was placed in an EDTA tube and kept at -20°C until the polymerase chain reaction amplification and detection of the vitamin D binding protein BP (rs2282679) gene (ARMS-PCR) method could be performed to avoid repetitive thawing and freezing. The remaining 3 ml were moved to a sterile gel tube, left to clot at room temperature, and then spun at 2500 rpm for 10 minutes to prevent the Vitamin D ELISA Kit (Mabtech USA) from undergoing repeated thawing and freezing. The serum that had been isolated was placed in Eppendorf tubes and quickly refrigerated at -20°C until it could be used again. With p=0.644 for gender and p=0.813 for age, the results did not show any significant differences between the groups. The current study showed (37.5%) of vitiligo patients have a positive family history, 25(OH) D level decreased in inpatients compared to control, and showed that the most abundant gene is AA in vitiligo patients compared to controls, and this enhanced decreased result of 25(OH) D. Also suggested an association between vitamin D binding protein BP rs2282679 polymorphism and the levels of vitamin D in vitiligo patient.

Keywords

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