Search
21 articles for “PCR”
-
Comparative Efficacy of Hybrid Capture 2 and Real-Time PCR in Detecting High-Risk HPV Genotypes for Cervical Cancer Screening
Abstract: Human papillomavirus (HPV) infection is a leading cause of cervical cancer, making early detection critical for effective prevention and treatment. Among the diagnostic methods available, Hybrid Capture 2 (HC2) and Real-Time Polymerase Chain Reaction (PCR) are widely used for detecting high-risk HPV genotypes, particularly HPV 16 and 18, which account for the majority of cervical cancer cases. This review aims to compare the efficacy of HC2 and Real-Time PCR in …
Published in International Journal of Genetic Modifications and Recombinations · Vol. 2, Issue 2, 2024 · pp. 13–17 Read article
-
Comparison of a Human Papillomavirus Test with the Hybrid Capture 2 and a Real-Time PCR Method for 18 High-Risk and Low-Risk Human Papillomavirus Infections in Women
Abstract: Squamous intraepithelial lesions (SILs), which are a kind of cancer, and HUMAN papillomavirus (HPV) viral load have frequently been investigated as a risk factor in findings that were in dispute. These studies made use of several HPV viral quantitation assays [including the commercially available hybrid capture 2 (HC2) assays], which vary in their capacity to consider variations in cervical cell collection, linear dynamic range of viral load quantitation, and identified …
Published in Research and Reviews: A Journal of Microbiology and Virology · Vol. 15, Issue 1, 2025 · pp. 10–16 Read article
-
A Study to Assess the Satisfaction Level of Children on Parent–Child Relationship Among Adolescent Girls Studying at Selected Educational Institutions in Honavar, Uttara Kannada
Abstract: Background: A strong parent-child relationship (PCR) is essential for a child’s overall development. This study aimed to explore the quality of parent-child relationships, particularly with fathers, among Indian adolescent girls. While societal attitudes have evolved, concerns remained about whether female children receive the same love, respect, and care as their male counterparts. Methodology: This research used a quantitative approach with a descriptive design. A total of 253 adolescent girls from …
Published in International Journal of Women's Health Nursing And Practices · Vol. 1, Issue 2, 2023 · pp. 14–20 Read article
-
A Comprehensive Review on COVID-19 Evolution and Development
Abstract: The emerging pandemic causing flu-like symptoms in patients is caused by the novel Coronavirus that was previously called 2019-nCoV. The mutated gene of SARS-CoV was transformed to SARS-CoV-2 in animals and further transmitted to humans in the month of December 2019 in Wuhan city of China. Eventually, the novel coronavirus spread all around the globe as a highly contagious disease. COVID-19 (the illness caused by coronavirus) has affected 213 countries …
Published in Research and Reviews : A Journal of Life Sciences Read article
-
Genetic Detection of Hepatitis C -Virus and Occult Hepatitis B in Patients from Al-Najaf Al-Ashraf Governorate, Iraq.
Abstract: Recently, a noticeable increase in the prevalence of occult Hepatitis B virus (HBV) and Hepatitis C virus (HCV) infections has been observed among clinical cases such as patients undergoing hemodialysis, blood transfusion, liver diseases, and thalassemia worldwide. To limit and control this spread, the present study was conducted to investigate and detect the molecular presence of HCV and occult HBV using the Nested PCR technique, as well as to observe …
Published in Research and Reviews: A Journal of Microbiology and Virology · Vol. 16, Issue 1, 2026 Read article
-
Molecular Characterization of Circulating Rota Viral Strain causing infantile diarrhoea in Tripura, North East India
Abstract: Introduction: North Eastern India is geographically distinct from rest of India and the trend of Rota viral diarrhoea among children below 5 years of age group is not well studied yet. The Government of India introduced the oral rotavirus vaccine (ROTAVAC) in 2016 as part of the Universal Immunization Programme in different parts of India. Objective: (1). To determine the proportion of circulating Rotavirus strains causing acute viral gastroenteritis (AGE) …
Published in Research and Reviews: A Journal of Microbiology and Virology · Vol. 11, Issue 3, 2021 · pp. 6–13 Read article
-
Application Of Silver Nanoparticles On Experimental Wound Infection Of Pseudomonas aeruginosa In Rats
Abstract: This study aimed to evaluate the application of silver nanoparticles on experimental wound infection of Pseudomonas aeruginosa in rats. Infected patients at a private hospital in Baghdad had fifty swab samples taken from their wounds and burns (25 samples in total). These swabs were cultured on primary medium and then routine bacteriological tests were done for identification. Molecular confirmation of bacteria was done by using 16S rRNA gene. The research …
Published in International Journal of Antibiotics · Vol. 2, Issue 1, 2025 · pp. 1–8 Read article
-
Molecular Surveillance of Spring Viraemia of Carp Virus (SVCV) and Koi Herpesvirus (KHV) Across Major Aquaculture Zones of Telangana, India
Abstract: Viral diseases represent a persistent threat to cyprinid aquaculture; however, region-specific surveillance data from tropical production systems remain limited. To address this gap, a comprehensive statewide active surveillance programme was undertaken across Telangana, India, from 2019 to 2022 to investigate the occurrence of two priority transboundary pathogens, Spring Viraemia of Carp Virus (SVCV) and Koi Herpesvirus (KHV). A total of 414 fish specimens were systematically collected from five surveillance sectors …
Published in International Journal of Virus Studies · Vol. 3, Issue 2, 2026 Read article
-
A Comprehensive Review on Monkeypox Infection
Abstract: Monkeypox represents a zoonotic viral affliction instigated by the monkeypox virus, which is classified within the Poxviridae family and is distinct from the virus that causes chickenpox. Initially recognized in 1958, this infection has recently attracted international scrutiny due to its escalating incidence beyond traditional endemic territories. This review delivers an exhaustive examination of monkeypox, encompassing its epidemiology, viral morphology, modes of transmission, pathogenesis, clinical presentations, and therapeutic interventions. The …
Published in Recent Trends in Infectious Diseases · Vol. 2, Issue 1, 2025 · pp. 23–32 Read article
-
Unmasking Human Metapneumovirus: The Emerging Threat to Vulnerable Populations
Abstract: Human Metapneumovirus (HMPV) is a contagious respiratory virus that commonly spreads among individuals. It can cause serious infections, especially in high-risk groups like infants, older adults, and individuals with weakened immune systems.It is a major contributor to acute respiratory illnesses. Since its discovery in 2001, HMPV has been identified as a common cause of both upper and lower respiratory tract illnesses, with clinical presentations ranging from mild symptoms to severe …
Published in International Journal of Virus Studies · Vol. 2, Issue 1, 2025 · pp. 16–24 Read article
-
Molecular Characterization Phylogenetic Analysis from E.coli Isolated from Human and Cows Raw Milk
Abstract: E. coli is a significant pathogen that contributes to the genesis of both human and animal diseases. 20 different E. coli isolates were found in milk, stool, and utensil samples. Depending on whether each E. coli strain has the gene, the chuA, and the DNA segment TspE4.C2 has joined. the intestinal groups A and B1 as well as the extraintestinal groups B2 and D. The findings of the PCR-based analysis …
Published in Research and Reviews : A Journal of Life Sciences · Vol. 15, Issue 3, 2025 · pp. 1–8 Read article
-
Facilitated Deferred Transmmission a Risk to Public Health of Globalization and Intercountry Adoptions of Pets
Abstract: Increased temperatures due to climate change may be facilitating that arthropods vectors of zoonotic diseases rapidly colonize new territories. 6472 dogs have been selected to check main Mediterraneum zoonotic vector-borne, two transmitted by fly bites, Leishmaniasis and Dirofilariasis, and tree transmitted by ticks, Ehrlichiosis, Anaplasmosis, and Babesiosis through IFA, ELISA, PCR and microscopy tests and calculate the ratio of this diseases hosts as well the destinations in north of Europe …
Published in Recent Trends in Infectious Diseases · Vol. 1, Issue 1, 2024 · pp. 21–26 Read article
-
Application of pET28a Vector for the Over Expression of Recombinant Human Tim23 Protein in E. coli and its Validation by Immunoblotting
Abstract: This study aimed to produce recombinant human Tim23 protein by molecular cloning, expression, and purification. Amplification of human TIM23 was carried out by polymerase chain reaction (PCR) and cloned into DH5α cells. The pET28a-human TIM23 was transformed into BL21DE3 and expressed the gene by IPTG induction. Recombinant pure human Tim23 antigen was prepared, and injected into rabbits and polyclonal antibodies were raised, which was confirmed by western blotting. The transformed …
Published in Research and Reviews : A Journal of Life Sciences · Vol. 14, Issue 1, 2024 · pp. 32–44 Read article
-
Association of Vitamin D Binding Protein BP rs2282679 Gene Polymorphism and Serum Levels of Vitamin D in Patients with Vitiligo
Abstract: For vitamin D to have an impact, the vitamin D receptor (VDR) must be expressed and activated in the nucleus. The VDR has several known genetic variants. Biological impacts can be caused by changes in DNA sequences known as "polymorphisms" that are common in the population. Vitiligo is a disease that causes loss of skin color in patches, and it is a chronic (long-lasting) autoimmune disorder loss of color. The …
Published in Research and Reviews : A Journal of Biotechnology · Vol. 14, Issue 2, 2024 · pp. 45–52 Read article
-
Common Diseases of Silkworm and Their Management – A Comprehensive Overview
Abstract: Silkworms (Bombyxmori L.), pivotal to the sericulture industry, are susceptible to various diseases that significantly impact cocoon production and quality. Common silkworm diseases include viral, bacterial, fungal, and protozoan infections. Prominent viral diseases, such as nuclear polyhedrosis (NPV) and cytoplasmic polyhedrosis (CPV) often cause mass mortality in larvae. Bacterial diseases, like flacherie, are typically induced by poor hygiene and environmental stress. Fungal infections, including muscardine caused by Beauveria bassiana, proliferate …
Published in International Journal of Insects · Vol. 2, Issue 1, 2025 · pp. 22–28 Read article
-
Molecular Mechanisms of Drug Metabolism in Anesthesia: A Pharmacogenomic Perspective
Abstract: Pharmacogenomics studies how a person’s genetic profile affects their reaction to drugs, which is vital in anesthesia. Anesthetic pharmacology depends significantly on drug metabolism, which involves complex biochemical processes that manage the absorption, distribution, metabolism, and excretion (ADME) of anesthetic drugs. The cytochrome P450 (CYP) enzyme family plays a critical role in the metabolism of various anesthetic drugs, with genetic polymorphisms leading to inter-individual variability in drug responses. Specific CYP …
Published in Research & Reviews: A Journal of Drug Formulation, Development and Production · Vol. 12, Issue 1, 2025 · pp. 90–95 Read article
-
COVID-19 Resurgence in 2025: India’s Continuing Public Health Challenge
Abstract: The year 2025 has witnessed the resurgence of COVID-19, reigniting concerns at both global and national levels regarding the persistent threat posed by SARS-CoV-2. In early 2025, India witnessed a moderate resurgence of COVID‑19, characterized by the emergence of Omicron sub-variants and a rise in mild-to-moderate infections. As of mid-June, active COVID‑19 cases in India totaled approximately 6,000–7,400, with hotspots in Kerala (~2,100 cases), Delhi (~600), Maharashtra (~500), and several …
Published in International Journal of Tropical Medicines · Vol. 2, Issue 2, 2025 · pp. 16–23 Read article
-
Immunological and Genetic Markers of Rheumatoid Arthritis Among Women in North India: A Case-Control Study
Abstract: Background: Rheumatoid arthritis (RA) is an autoimmune disease systemically afflicting women marked by chronic inflammation around the involved joints leading to joints destruction. It has both endocrinological and immunological associations as well as genetic associations but data pertaining to women of North India is scanty. Objective: This case-control study was done to evaluate the presence and significance of major immunological and genetic markers of RA in North Indian women with …
Published in International Journal of Genetic Modifications and Recombinations · Vol. 3, Issue 2, 2025 · pp. 1–10 Read article
-
A Review on Unmasking Merkel Cell Polyomavirus: From Discovery to Cancer Driver
Abstract: Merkel cell polyomavirus (MCPyV) is a small, non-enveloped, circular double-stranded DNA virus belonging to the Polyomaviridae family, first characterized in 2008. Epidemiological data suggest widespread exposure, with seroprevalence rates approaching 80% in adults. While primary infection is typically asymptomatic, rare integration events in Merkel cells can initiate oncogenesis, yielding Merkel cell carcinoma (MCC), a neuroendocrine skin cancer with a five-year survival rate under 60%. This review integrates findings from six …
Published in International Journal of Virus Studies · Vol. 2, Issue 2, 2025 · pp. 6–11 Read article
-
CRISPR, Recombinant DNA, and LAMP-Based Platforms for Monkeypox: Emerging Tools for Diagnosis and Therapeutic Development
Abstract: Monkeypox, a zoonotic viral infection caused by the Monkeypox virus of the Orthopoxvirus genus, has recurred as a worldwide public health concern after recent outbreaks outside of its classical endemic areas in Central and West Africa. The resurgence has demanded a thorough reassessment of its epidemiology, mode of transmission, and clinical presentation. In light of these challenges, recombinant DNA technology (rDNA) has emerged as a candidate for developing vaccines, diagnostics, …
Published in International Journal of Virus Studies · Vol. 3, Issue 1, 2026 · pp. 7–28 Read article