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56 articles for “Chromatographic separations”
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Review on UV – Visible Spectroscopy and HPLC
Abstract: Like Fourier-transform infrared spectroscopy, UV-visible spectroscopy is used to determine the purity of pharmaceutical compounds. Many substances have chromospheres, which absorb certain wavelengths of ultraviolet or visible light. Using the Beer–Lambert equation, the absorption of spectra obtained from these samples at given wavelengths can be directly related to the sample concentration. Normally, UV and UV-visible spectra are recorded at high and low pH, and the results of both are compared …
Published in Journal of Modern Chemistry & Chemical Technology · Vol. 13, Issue 1, 2022 · pp. 43–54 Read article
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HPTLC Method Development and Validation of Genistein and Daidzein Estimation in Mucuna cochinchinensis Seed Extracts
Abstract: Aim: The current study was designed with the objective to estimate the concentration of Genistein and Daidzein in ethyl acetate extract of Mucuna cochinchinensis by high-performance thin-layer chromatography (HPTLC).Methods: The separation of bioactive compounds was performed using mobile phase, Toluene: n-Butanol: Glacial Acetic Acid: Water (1:4:2:2) and detected at wavelength 254 nm. The method was validated for linearity, accuracy, precision, limit of detection (LOD) and limit of quantification (LOQ) by …
Published in Research & Reviews: A Journal of Drug Formulation, Development and Production · Vol. 7, Issue 1, 2020 · pp. 16–25 Read article
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Enhanced Iron Removal in Experimental Iron Overload by Nanoniosomes of Desferrioxamine
Abstract: This preliminary study explores the feasibility of employing nanocapsules prepared from nonionic surfactant vesicles (niosomes) as a carrier for an iron-chelating agent, Desferrioxamine (DFO, Desferal), to enhance iron excretion from the body. The methodology consisted of creating an iron overload model in an experimental animal followed by measurement of iron excretion pattern in the urine by atomic absorption spectrophotometry. Niosomes were prepared with span 60: cholesterol:dicetyl phosphate by the lipid …
Published in Research and Reviews: A Journal of Pharmaceutical Science · Vol. 5, Issue 1, 2014 · pp. 14–20 Read article
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A Comprehensive Review on the Miniaturized Analytical Techniques for Sustainable Pharmaceutical Testing
Abstract: Miniaturized analytical techniques are revolutionizing pharmaceutical testing by offering sustainable and efficient alternatives to traditional methods. These techniques are designed to reduce sample and reagent consumption, minimize waste generation, and accelerate analysis, aligning perfectly with the principles of green analytical chemistry. This comprehensive review aims to thoroughly explore the landscape of miniaturized analytical techniques, specifically highlighting their profound impact on fostering sustainable practices within the pharmaceutical sector. The review systematically …
Published in Research and Reviews: A Journal of Pharmaceutical Science · Vol. 17, Issue 1, 2026 · pp. 18–39 Read article
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Chemiluminescence: Recent Developments and Photochemical Applications in Analytical Chemistry
Abstract: Chemiluminescence (CL) is the observable production of electromagnetic radiation from a chemical reaction, typically in the form of ultraviolet, visible, or infrared light. According to subjective yield, CL has either been directly emitted from electronically excited intermediate products, or it has been supported by another molecule to emit CL. Since the 1950s, CL has been supported as a valid analytical tool, with the descriptive issued scope being limited to content …
Published in International Journal of Photochemistry and Photochemical Research · Vol. 2, Issue 1, 2024 · pp. 20–25 Read article
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A Systematic Review of Method Development and Validation for Ramipril Analysis Using Hplc in Cardiovascular Research
Abstract: The field of analytical chemistry encompasses a variety of methods that are crucial in determining the chemical composition of different substances. UV-visible spectroscopy and high-performance liquid chromatography (HPLC) are two widely utilized methods that are the subject of this article. UV-visible spectroscopy uses the Beer-Lambert law to quantify light absorption, which can provide information about the concentration of absorbing species. The method is based on how light and matter interact, …
Published in Journal of Modern Chemistry & Chemical Technology · Vol. 15, Issue 2, 2024 · pp. 37–44 Read article
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A Comprehensive Review of Analytical Method – Development and Method Validation of Digoxin Tablets
Abstract: The main objective of this review is to give an outline of the method development and validation process of digoxin, and this review covers the different types of spectroscopic and chromatographic techniques, such as HPLC, UHPLC-Tandem mass spectroscopy, TLC, and LC-MS. A novel method for the determination of digoxin in biological samples has been developed and validated, focusing on improving sensitivity, specificity, and reproducibility. The method was validated in terms …
Published in Research & Reviews: A Journal of Drug Formulation, Development and Production · Vol. 12, Issue 1, 2025 · pp. 47–58 Read article
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Derivatization of Chiral Compounds with Chiral Reagents and Separation their Isomers by HPLC System
Abstract: Chiral compounds such as alanine amino acid and s-aminethylated L-cystiene derivatized with chiral reagents such as Marfey's reagents, 2,3,4,6-tetra-O-acetyl-b-D-glucopyranosylisothiocyanate (GITC) and 2,3,4,6-tetra-O-benzoyl-b-D-glucopyranosylisothiocyanate (BGIT), were achieved and separated by optimizing good methods using high performance liquid chromatography. Keywords: Derivatization, amino acid, chiral reagent, HPLCCite this Article Khlafulla Abdulrahim M, Maga Rajab A, Elmessmary Mohamad F. Derivatization of Chiral Compounds with Chiral Reagents and Separation their Isomers by HPLC System. Research & …
Published in Research and Reviews: A Journal of Pharmacology · Vol. 6, Issue 2, 2016 · pp. 39–43 Read article
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Method Development and Validation by HPLC: A Brief Review
Abstract: High-performance liquid chromatography (HPLC) is a significant analytical technique, usually used for the determination and separation of pharmaceutical and biological samples. This is most adaptable, safe, reliable, and fast chromatographical method for quality control of drug samples. The objective behind the present study was to focus on different features of HPLC, such as principle, types, instrumentation, HPLC method development and validation along its application.
Published in Research and Reviews: A Journal of Pharmaceutical Science · Vol. 12, Issue 1, 2025 · pp. 27–39 Read article
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Formulation Boswellia serrata and Commiphora mukul Extract Gel for Topical Inflammation
Abstract: Inflammation and rheumatism remain serious health problems in the present era. In the present attempt, the authors developed anti-inflammatory gel which gives benefit of faster permeation for quicker action. This anti-inflammatory gel is very useful for the arthritis patients. For the formulation purpose Boswellia serrata (Shallaki) and Commiphora mukul (Guggul) were selected because very less work has been done on these plants and for synergistic effect known of herbal extracts …
Published in Research & Reviews : Journal of Herbal Science · Vol. 4, Issue 2, 2015 · pp. 1–8 Read article
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Analytical Method Development and Validation for the Simultaneous Estimation of Elvitegravir and Cobicistat by RP-HPLC Method
Abstract: A new method was established for simultaneous estimation of Elvetigravir and Cobicistat by RP-HPLC method. The chromatographic conditions were successfully developed for the separation of Elvetigravir and Cobicistat by using Xterra C18 5 µm (4.6×150 mm) column, flow rate was 1 ml/min, mobile phase ratio was phosphate buffer (0.05 M) pH 7: MEOH (30:70%v/v) (pH was adjusted with orthophosphoric acid), and the detection wave length was 270 nm. The instrument …
Published in Research & Reviews: A Journal of Drug Formulation, Development and Production · Vol. 5, Issue 2, 2018 · pp. 1–6 Read article
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Determination of RP-HPLC Method for Safinamide: Its Bulk and Tablet Dosage Form
Abstract: A new method was established for estimation of Safinamide by RP-HPLC method. The chromatographic conditions were successfully developed for the separation of Safinamide by using Agilent C18 column (4.6×150 mm, 5 µ); flow rate was 1 ml/min, mobile phase ratio was Methnol, Acetonitril (60:40), pH 3 (pH was adjusted with orthophosphoric acid), detection wavelength was 235 nm. The instrument used was WATERS HPLC Auto Sampler, Separation module 2695, photo diode …
Published in Research & Reviews: A Journal of Drug Formulation, Development and Production · Vol. 5, Issue 3, 2018 · pp. 1–6 Read article
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Development of Anti IgG Polyclonal Antibody Against Canine IgG and Their Roles in Diagnostic Purposes
Abstract: IgG plays an important part in immunological studies and also in living bodies for providing secondary immune response which triggers as passive immunization against harmful pathogens in dogs. Purpose: The aim of this investigation is to separate, identify, and refine immunoglobulin G from canine serum. Methods: Ammonium sulphate precipitation was used to separate the dog's serum, which was then concentrated over night in a dialysis bag inside of a refrigerator. …
Published in International Journal of Vaccines · Vol. 1, Issue 2, 2024 · pp. 01–06 Read article
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Utilizing Metallurgical Wastes for the use of magnetic separation
Abstract: To enable a wider application of magnetic applications, concentration and purification parameters must be enhanced. The inventive fusion of electromagnetic gradients and multipurpose separations will establish new magnetic-based trends for large-scale downstream processing. More creative solutions are required for downstream processing to advance and resolve the problems facing bioprocessing today. Chromatography is the method that a conservative industry sector uses the most frequently. Chromatography provides a few benefits, but it …
Published in Emerging Trends in Chemical Engineering · Vol. 9, Issue 2, 2022 · pp. 37–41 Read article
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Screening of Phytochemical Constituents Towards Antimicrobial Potency of Hygrophila schulli
Abstract: The present study was carried out to determine the invitro antibacterial and antifungal and phytochemical activity of Hygrophila schulli. In this study antimicrobial activity of leaves of Hygrophila schulli was studied by analyzing in different concentrations ranging from 100-500µg. We observed that at higher concentrations (>300 µg) ethanolic extract of Hygrophila schulli showed certain significant antimicrobial effect. Antimicrobial activities against Escherichia coli, Fusarium verticillioides, Klebsiella sp., Aspergillus flavus, Pseudomonas aeruginosa, …
Published in Research and Reviews : A Journal of Biotechnology · Vol. 1, Issue 1, 2011 · pp. 1–5 Read article
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Screening of Secondary Metabolites for Estimating Antimicrobial and Antioxidant Potency of Clitoria ternatea (L.)
Abstract: The present study was carried out for the screening of secondary metabolites for estimating the antimicrobial potency of Clitoria ternatea (L.). In this study antimicrobial activity of leaves of Clitoria ternatea were studied by analyzing in different concentrations ranging from 100–500 µg. We observed that at higher concentrations (>300 µg) ethanolic extract of Clitoria ternatea showed certain significant antimicrobial effect. Antimicrobial activities against Escherichia coli, Fusarium verticillioides, Klebsiella sp., Aspergillus …
Published in Research and Reviews : A Journal of Biotechnology · Vol. 1, Issue 1, 2011 · pp. 1–6 Read article
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Quantitative Determination of Isomeric Components of essential oils by RP-HPLC-PDA in multi-herbal tablets
Abstract: Essential oils are lipophilic and highly volatile secondary plant metabolites, principally mono- and sesquiterpenes. The widespread usage and applications in biopharmaceutics and cosmoceutics of these components drives their quality assessments criteria to be established. Gas chromatography is one of the predominantly used technique for analyzing volatile oils. Although Gas Chromatography employs estimation designs operated at elevated temperatures leading for plausible structural alterations of thermo labile compounds. Hence, a novel gradient …
Published in Research and Reviews: A Journal of Pharmaceutical Science · Vol. 11, Issue 3, 2020 · pp. 19–28 Read article
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Supplementing Wheat Protein to Improve and Complement Its Nutritional Quality
Abstract: Protein concentrates from lobia seeds, soybean, hyacinth beans and milk (casein and whey) were prepared following isoelectric precipitation. The percent moisture/protein content of these concentrates was 5.01±0.03/84.81±2.19; 5.15±0.06/86.60±1.66; 5.11±0.03/83.28±1.42, 8.19±0.15/86.03±0.68 and10.21±0.04/77.5±0.68 respectively. Acidic and alkaline hydrolysis of these concentrates was carried out in steam under pressure conditions (121°C) at different time intervals (60, 120 and 180 min). Hydrolysis treatment in both the conditions for 180 min resulted into improved separation …
Published in Research & Reviews : Journal of Food Science & Technology · Vol. 4, Issue 2, 2015 · pp. 13–20 Read article
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Extraction of Essential Oil and Anti-bacterial Activity Determination of Cassia tora Seed on some Selected Microorganisms (Staphylococcus aureus, Trichophyton rubrum and Streptococcus species)
Abstract: Essential oils have been used for extensive applications of variety of wellness throughout documented history. With massive advancement in science, essential oils today have undergone numerous refining methods to give an improved effect. The aim of this study was to extract and investigate the functional properties of Cassia tora seed oil through analytical profiling using gas chromatography. In the gas chromatographic analysis two chromatographic conditions used were the same except …
Published in Research and Reviews : A Journal of Biotechnology · Vol. 10, Issue 3, 2020 · pp. 12–20 Read article
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A Novel Technique for Stability Testing of Alliin and Sennosides in Unani Formulations
Abstract: Unani system of medicines is a science which deals with the preventive and promotive aspects of health of human beings and health problems that arise due to factors like ecological and/or environmental, which include humours categorized as blood, phlegm, yellow bile and black bile, and different biological fluids circulating in various vessels of the body. There is significant mandate in drug testing for a specific and precise analytical method for …
Published in Research & Reviews : A Journal of Unani, Siddha and Homeopathy · Vol. 1, Issue 1, 2014 · pp. 1–5 Read article