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Research & Reviews : Journal of Food Science & Technology

Supplementing Wheat Protein to Improve and Complement Its Nutritional Quality

  1. Arvind Jaiswal
  2. Dev Kumar Yadav
  3. P.E. Patki
  4. R.K. Gupta

Abstract

Protein concentrates from lobia seeds, soybean, hyacinth beans and milk (casein and whey) were prepared following isoelectric precipitation. The percent moisture/protein content of these concentrates was 5.01±0.03/84.81±2.19; 5.15±0.06/86.60±1.66; 5.11±0.03/83.28±1.42, 8.19±0.15/86.03±0.68 and10.21±0.04/77.5±0.68 respectively. Acidic and alkaline hydrolysis of these concentrates was carried out in steam under pressure conditions (121°C) at different time intervals (60, 120 and 180 min). Hydrolysis treatment in both the conditions for 180 min resulted into improved separation of amino acids. Identification of essential amino acid with reference to the standard was performed using thin layer chromatography with the help of n-Butanol: acetic acid: water: 4:1:1 and Phenol:Water: 75:25 as solvent systems. Whey and casein protein concentrates were found to be cost effective biologically potentialsource of essential amino acids hence these were used to fortify wheat flour protein. To Increase essential amino acid content of wheat flour in relation to meet 100% RDA requirement of a complete and balance protein, 15.0 g casein or 23.0 g whey protein per 100 g sample wasrequired. Wheat based products such as chapaties were prepared using specified amount of casein / whey protein concentrates. The resulted product was well acceptable having better nutrition with respect to essential amino acid and protein efficiency ratio. Keywords: Fortification, protein concentrates, RDA, essential amino acid, chapatiesCite this Article Arvind Jaiswal, Dev Kumar Yadav, Patki PE, et al. Supplementing Wheat Protein to Improve and Complement its Nutritional Quality. Research & Reviews: Journal of Food Science and Technology. 2015; 4(2): 13–20p

References (36)

  1. Trottier N, Walker R. Nutritional Aspects of Proteins. Appl. Food Protein Chem. 2014; 113–25p.
  2. RDA-2010. (Cited 25 Apr 2015 ); Available from: http://www.icmr.nic.in/fin al/RDA-2010.pdf.
  3. Damodaran S. Amino Acids, Peptides, and Proteins. In: Damodaran S, Parkin KL, Fennema OR, (Ed.) Fennema's Food Chemistry. Florida, USA: CRC Press. 2007.
  4. Young VR, Pellett PL. Plant Proteins in Relation to Human Prote in and Amino Acid Nutrition. Am. J Clin Nutr. 1994; 59: 1203–10p.
  5. Harper A, Benevenga N. Effect of Disproportionate Amounts of Amino Acids. In: Lawrie RA (Ed.) Proteins as Human Food. Westport, Conn., USA: Avi Publishing Co. 2013.
  6. Reid M, Badaloo A, Forrester T, et al. In vivo Rates of Erythrocyte Glutathione Synthesis in Children with Severe Protein- Energy Malnut rition. Am. J. Physiol. Endocrinol Metab.. 2000; 278: 405–8p.
  7. Wu G, Wu Z, Dai Z, et al. Dietary Requirements of “ Nutritionally Non - Essential Amino Ac ids” by Animals and Humans. Amino Acids. 2013; 44: 1107–7p.
  8. Ragaee S, Abdel -Aal E -SM, Noaman M. Antioxidant Activity and Nutrient Composition of Selected Cereals for Food Use. Food Chem. 2006; 98: 32–8p.
  9. Poletti S, Gruissem W, Sautter C. The Nutritional Fortif ication of Cereals. Curr. Opin. Biotechnol. 2004; 15: 162–4p.
  10. Berner LA, Clydesdale FM, Douglass JS. Fortification contributed greatly to vitamin and mineral intakes in the United States, 1989–1991. J. Nutr. 2001; 131: 2177–7p.
  11. Jansen GR. Armino Acid Fortification. In: Altschul AM, Wilcke HL, (Ed.) New Protein Foods: Animal Protein Supplies. New York: Academic Press. 2013.
  12. Egbuna K, Ajibaye O, Orok A, et al. Optimization of the Protein Nutritive Value of Wheat/Cassava Breadmix by Supplementing with Limiting Amino - Acids (L -Lysine and L-Methionine). Niger. J. Parasitol. 2014; 32–6p.
  13. Alu’datt MH, Alli I, Nagadi M. Preparation, Characterization and Properties of Whey- Supplementing Wheat Protein to Improve its Quality Jaiswal et al. RRJoFST (2015) 13-20 © STM Journals 2015. All Rights Reserved Page 20 Soy Proteins Co -Precipitates. Food Chem. 2012; 134: 294–7p.
  14. Wangoh J, Farah Z, Puhan Z. Iso -Electric Focusing of Camel Milk Proteins. Int. Dairy J. 1998; 8: 617–5p.
  15. AOAC. Official Methods of Analysis . Washington DC: Association of Official Analytical Chemists, 1984.
  16. Bovier HP. Hawk’s Physiological Chemistry. In: Oser BL, (Ed.) Hawk’s Physiological Chemistry. New York: McGraw-Hill Book Co. 1965.
  17. Classics Lowry OH, Rosebrough NJ, Farr AL, et al. Protein Measurement with the Folin Phenol Reagent. J. Biol. Chem. 1951; 193: 265–11p.
  18. Waterborg JH. The Lowry Method for Protein Quantitation. In: Walker JM. (Ed.) The Protein Protocols Handbook. Totowa, New Jersey: Humana Press Inc. 2009.
  19. Badadani M, SureshBabu S, Shetty KT. Optimum Conditions of Autoclaving for Hydrolysis of Proteins and Urinary Peptides of Prolyl and Hydroxyprolyl Residues and HPLC Analysis. J. Chromatogr. B. 2007; 847: 267–8p.
  20. Murray JM, Delahunty CM, Baxter IA. Descriptive Sensory Analysis: Past, Present and Future. Food Res. Int. 2001; 34(6): 461– 11p.
  21. Duncan DB. Multiple Range and Multiple F Tests. Biometrics 1955; 11: 1–42p.
  22. Mishra N, Chandra R. Development o f Functional Biscuit from Soy Flour and Rice Bran. Int. J. Agric. Food Sci. 2012; 2: 14–7p.
  23. Asif M, Rooney LW, Ali R , et al . Application and Opportunities of Pulses in Food System: A Review. Crit. Rev. Food Sci. Nutr. 2013; 53(11): 1168–11p.
  24. Kalman DS. Amino Acid Composition of an Organic Brown Rice Protein Concentrate and Isolate Compared to Soy and Whey Concentrates and Isolates. Foods. 2014; 3: 394–9p.
  25. Fox PF, McSweeney PL. Dairy Chemistry and Biochemistry. Springer Science & Business Media. 1998: 146–88p.
  26. Walstra P, Geurts TJ, Noomen A, et al. Dairy Technology: Principles of Milk Properties and Processes. New York: Marcel Dekker, Inc. 2013; 71–16p.
  27. Weiss M, Manneberg M, Juranville J -F, et al. Effect of the Hydrolysis Method on the Determination of the Amino Acid Composition of Proteins. J. Chromatogr. A. 1998; 795: 263–13p.
  28. Sales M, De Freitas O, Zucoloto S, et al. Casein, Hydrolyzed Casei n, and Amino Acids that Simulate Casein Pro duce the Same Exten t of Mucosal Adaptat ion to Massive Bowel Res ection in Adult Ra ts. Am. J. Clini. Nutr. 1995; 62: 87–6p.
  29. Sindayikengera S. Nutritional Evaluation of Caseins and Whey Protei ns and Their Hydrolysates from Protamex. J. Zhejiang Uni. Sci. B. 2006; 7: 90–9p.
  30. Nilsson M, Holst JJ, Björck IM. Metabolic Effects of Amino A cid Mixtur es and Whey Protein in Healthy Subjects: Studies Using Glucose -Equivalent Dri nks. Am. J. Clini. Nutr. 2007; 85: 996–9p.
  31. Tipton KD, Wolfe RR. Protein and Amino Acids for Athletes. J. Sports Sci. 2004; 22: 65–14p.
  32. Shewry P. Wheat. J. Exp. Bot. 2009; 60: 1537–7p.
  33. Scherz H, Senser F. Food Composition and Nutrition Tables. Medpharm GmbH Scientific Publishers. 1994; 301–39p.
  34. Belitz H -D, Grosch W, Schieberle P. Cereals and Cereal Products. Food Chem. 2009; 670–76p.
  35. Ha E, Zemel MB. Functional Properties of Whey, Whey Compon ents, and Essential Amino Acids: Mechanisms Underlying Health Benefits for Active Pe ople (Review). J. Nutr. Biochem. 2003; 14: 251–8p.
  36. Horax R, Hettiarachchy N, Chen P, et al. Preparation and Characterization of Protein Isolate from Cowpea (Vigna unguiculata L. Walp .). J. Food Sci. 2004; 9: 114–5p. Cite this Article Arvind Jaiswal, Dev Kumar Yadav, Patki PE , et al . Supplementing Wheat Protein to Improve and Complement its Nutritional Quality. Research & Reviews: Journal of Food Science and Technology. 2015; 4(2): 13–20p.

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