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27 articles for “protease enzyme”
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BIOCHEMICAL AND KINETICS STUDIES ON PROTEASE ENZYME ISOLATED FROM AYURVEDIC HERBS ALONG WITH THEIR APPLICATION STUDIES
Abstract: The objective of the present work was to conduct comprehensive biochemical and kinetic analysis of a protease enzyme extracted from two Ayurvedic herbs, namely Shankhpushpi (Convolvulus prostratus) and Mulethi (Glycyrrhiza glabra). The primary focus was to characterize various biochemical aspects of the enzyme, encompassing substrate specificity, optimal pH and temperature conditions, stability, and activation energy. Furthermore, crucial kinetic parameters such as Michaelis-Menten constant (Km) and maximum reaction velocity (Vmax) were …
Published in Research and Reviews : A Journal of Biotechnology · Vol. 13, Issue 3, 2023 · pp. 21–37 Read article
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In Silico Identification of Small Molecules as Novel Coronavirus Protease Enzyme Inhibitors
Abstract: AbstractThe novel corona virus 2019 (COVID-19) is one of the global threats to the human civilization as it causes serious respiratory infection and multi-organ failure. Because of the absence of appropriate treatment protocol for 2019 novel corona virus, different methodologies are being tried in China, including drug repurposing. Recent discovery revealed the structure of novel corona virus main protease enzyme (Mpro) containing HIS41 and CYS145 amino acid residues present in …
Published in Research & Reviews: A Journal of Bioinformatics · Vol. 7, Issue 2, 2020 · pp. 33–43 Read article
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Isolation and Characterization of Protease Producing Organism Bacillus Species from Slaughter House Waste
Abstract: The purpose of this investigation was to isolate protease producing bacteria from slaughter house waste collected in local area of Kaliakkavilai. The Bacillus sp. was identified by several biochemical and physiological tests. The effect of different production parameters such as pH, temperature, metal ions concentration and substrate concentration for protease production by the isolated bacterial strain were studied. The enzyme production was assayed in submerged fermentation with optimized parameters to …
Published in Research and Reviews: A Journal of Microbiology and Virology · Vol. 3, Issue 3, 2013 · pp. 8–14 Read article
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The Role of Quercetin, Curcumin, Eugenol, and Andrographolide in COVID-19 Treatment: A Review of Their Mechanisms of Action and Clinical Efficacy
Abstract: The world has recently experienced the covid diseases. It became the global worry with no remedy initially. Later, different antiviral medications in the form of injections and nasal sprays began to be employed. Many studies have been conducted to determine the antiviral effects of phytochemical substances. The corona virus's spike protein interacts with the ACE2 receptors on the alveoli's surface to enter the cell. After that, the polyprotein is transformed …
Published in Research and Reviews : A Journal of Biotechnology · Vol. 13, Issue 1, 2023 · pp. 27–38 Read article
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Understanding the Molecular Mechanisms of Proteases in Bioprocessing: A Review on the Future of the Food Industry
Abstract: Proteases are enzymes that break down proteins through peptide bond catalysis. A protease is a single polypeptide chain of some 250 amino acids and is devoid of sulfhydryl groups. The COOH-terminal tryptic peptide of the protease molecule contains some 43 residues. Because of this unique structure and mechanism, they play a crucial role in the Bioprocessing industry, especially in food processing applications. Herein, the sources of proteases are discussed as …
Published in Research and Reviews : A Journal of Biotechnology · Vol. 13, Issue 2, 2023 · pp. 17–25 Read article
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Functional Role of Feed Enzymes in Broiler Diets: A Pathway to Enhanced Nutrient Utilization, Performance, Health and Welfare
Abstract: The inclusion of feed enzymes in broiler diets has emerged as a pivotal strategy for improving nutrient utilization, enhancing growth performance, and promoting overall health and welfare in modern poultry production. Feed enzymes, such as phytase, xylanase, and protease, facilitate the breakdown of complex molecules in feed ingredients, leading to increased bioavailability of essential nutrients and improved digestion efficiency. This review explores the underlying mechanisms by which feed enzymes contribute …
Published in International Journal of Biochemistry and Biomolecule Research · Vol. 3, Issue 2, 2025 · pp. 9–17 Read article
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Comparison of the Keratinase Gene Sequences of Fast, Slow and Intermediate Feather Degrading Strains of Bacillus licheniformis
Abstract: Feathers are composed of beta keratin which is an insoluble protein resistant to degradation. Bacillus licheniformis has a gene that codes for a keratinase enzyme that hydrolyzes feathers. The DNA sequences of the genes coding for keratinase enzymes of various strains of B. licheniformis were compared. Some strains of the bacteria hydrolyze feathers rapidly, some slowly, and some at an intermediate rate. The mature keratinase enzyme is composed of 274 …
Published in Research and Reviews: A Journal of Microbiology and Virology · Vol. 5, Issue 3, 2015 · pp. 1–9 Read article
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A Review on Environmental Pollution Mitigation by Fungal Proteases
Abstract: Proteases comprise a vital group of proteolytic enzymes with variety of functions. These enzymes possess the enormous commercial potential and have been used in several industrial processes, including food industry, leather processing, detergent industry and therapeutic applications. As well as they are used as an environmental pollution cleaning agent. Proteases are the replacement of conventional method of silver recovery from the X-ray films. Among the microbial enzymes only proteases constitute …
Published in Research and Reviews: A Journal of Microbiology and Virology · Vol. 7, Issue 3, 2017 · pp. 32–37 Read article
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A Review on Microbial Enzymes in the Food Industry: Current Innovations and Future Prospects
Abstract: Over the past few decades, microbial enzymes have become indispensable biocatalysts in food production, fundamentally revolutionizing how we approach manufacturing processes while simultaneously enhancing the quality of what we produce. Today, approximately 200 of the 4,000 known enzymes are deployed commercially with proteases, amylases, lipases, and lactases leading the charge in industrial food applications. This review brings together the latest innovations in how we use microbial enzymes for food processing, …
Published in Research & Reviews : Journal of Food Science & Technology · Vol. 15, Issue 1, 2026 · pp. 43–53 Read article
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Pathogenesis, Immune Response and Vaccine Development in Amoebiasis
Abstract: Worldwide, approximately fifty million cases of invasive E histolytica disease occur each year and resulting in more than 100,000 deaths. It is the second leading cause of mortality due to a protozoan parasitic infection after malaria. Entamoeba histolytica infection is associated with considerable morbidity and mortality in the form of intestinal and extra-intestinal amoebiasis especially in developing countries. Recent molecular studies have conclusively proved that there are morphologically similar species …
Published in Research and Reviews : A Journal of Immunology · Vol. 5, Issue 3, 2015 · pp. 30–35 Read article
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Aspergillus Oryzae: Multifunctional Powerhouse Revolutionizing Food Industries and Gut Health
Abstract: Aspergillus oryzae, commonly known as “Koji mold,” is a filamentous fungus extensively utilized in various food industries due to its robust enzymatic activity and generally recognized as safe (GRAS) status. This review explores its diverse applications, focusing on its role in fermented food production, enzyme generation, and contributions to gut health. In the production of fermented foods, such as soy sauce, miso, and sake, A. oryzae serves as a crucial …
Published in International Journal of Fungi · Vol. 2, Issue 1, 2025 · pp. 1–17 Read article
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Production of Oil from Agro Seeds using Mechanothermal Extraction: A Review of Chemical, Pharmaceutical, and Food Applications
Abstract: The increasing demand for sustainable raw materials and renewable industrial resources has intensified research into the extraction of oils from agro seeds for chemical, pharmaceutical, and food applications. Agro-seed oils are valuable due to their biodegradability, nutritional composition, eco-friendliness, and wide industrial applicability. This review examines the production of oil from agro seeds using mechanothermal extraction techniques with emphasis on extraction efficiency, physicochemical characteristics, and industrial utilization. Mechanothermal extraction combines …
Published in Journal of Catalyst & Catalysis · Vol. 13, Issue 2, 2026 · pp. 09–26 Read article
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Bioprocessing of Used X-ray Films for Silver Recovery by Thermoalkaliphilic Protease from Thermus aquaticus YT-1
Abstract: An environmentally benign enzymatic method for recovery of silver and polyethylene from used X-ray films was developed. This industrially scalable enzymatic process for the recovery of silver was investigated using the thermophilic alkaline protease from Thermus aquaticus YT-1. The rate of gelatin hydrolysis of X-ray film increased with the temperature till 70 ºC, suggesting enzyme stability till 70 ºC. Further, pH 10 was optimum for this process. Transparent polyethylene film …
Published in Research and Reviews : A Journal of Biotechnology · Vol. 2, Issue 2, 2012 · pp. 30–38 Read article
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A Review: A Source of Industrially Important Enzymes from Marine Actinomycetes
Abstract: Actinomycetes are Gram-positive, spore-forming, filamentous actinobacteria which usually contain G+C content of about 57–75% in their DNA. Actinomycetes are considered as a unique group of a microorganism having the characters of the true bacteria and the true fungi. Actinomycetes that have been detected from extreme conditions are considered to be sources of new enzymes of great industrial potential. They produce valuable secondary metabolites and industrially significant enzymes like pectinase, amylase, …
Published in Research and Reviews : A Journal of Biotechnology · Vol. 10, Issue 2, 2020 · pp. 20–25 Read article
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Production of High Yield Amylase and Protease by Aspergillus oryzae using Solid-State Fermentation
Abstract: Aspergillus oryzae strains were isolated from different sources and its ability to produce industrial scale amylase and protease using solid-state fermentation was carried in the present study. Different fermentation substrates such as rice bran and wheat bran were evaluated for maximum enzyme yield. Enzyme production was maximum on rice bran and it was achieved with optimized fermentation parameter such as temperature 30°C, pH 4–6, moisture content 60%, inoculum size 0.2 …
Published in Research and Reviews: A Journal of Microbiology and Virology · Vol. 8, Issue 3, 2018 · pp. 71–78 Read article
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Isolation and Screening of Some Protease Producing Bacteria to Treat Paper and Pulp Effluent
Abstract: Industrial pollution is one of the most alarming threats to the environment and paper and pulp mill is one of the prime contributors to it. Paper and pulp mill effluent affects biotic and abiotic components of the environment. Different methods are being employed for the treatment, but the biological method is found to be more efficient in terms of colour removal and for reduction in the value of BOD, COD, …
Published in Research and Reviews : A Journal of Life Sciences · Vol. 9, Issue 2, 2019 · pp. 11–16 Read article
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Aureobasidium pullulans- The Versatile Microcolonial Fungus for Industrial Production of Natural Products
Abstract: Aureobasidium pullulans, a yeast-like fungus, exhibits a remarkable capacity for synthesizing an extensive array of bioproducts with diverse industrial applications. This fungus is known to survive in several environmental conditions, making it a robust organism for biotechnological processes. The organism is characterized by its adaptability to various environmental conditions and habitats. It produces an array of extracellular enzymes such as amylases, lipases, proteases, and pectinases, contributing to its role as …
Published in Research and Reviews: A Journal of Microbiology and Virology · Vol. 14, Issue 1, 2024 · pp. 19–31 Read article
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A Novel Protease Identified in the Latex of Ficus longifolia
Abstract: AbstractA proteolytic enzyme has been extracted from the latex of the plant Ficus longifolia. The optimum pH and temperature for the activity of the enzyme have been determined as 9 and 70°C respectively. It was activated by cysteine and β-mercaptoethanol. Its activity was inhibited strongly and reversibly by PCMB (Para Chloro Mercury Benzoate), which revealed that the latex protease is a cysteine protease. It is a novel plant latex protease.Keywords: …
Published in Research & Reviews : Journal of Herbal Science · Vol. 8, Issue 3, 2019 · pp. 13–19 Read article
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Bacillus amyloliquefaciens: A Review
Abstract: Bacillus amyloliquefaciens is one of the many Bacillus species which is used as a probiotic, exerting health benefits to the host. B. amyloliquefaciens is a gram-positive, rod shaped bacteria with peritrichous flagella. The species also produces enzymes viz. cellulases, amylases, and proteases, which aid in digestion. The species is principally utilized as a feed additive substance for domestic animals and birds. It is gaining importance as plant growth promoter rhizobacteria …
Published in Research and Reviews: A Journal of Microbiology and Virology · Vol. 11, Issue 1, 2021 · pp. 9–17 Read article
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Rheumatoid Arthritis and Stem Cell Therapies Perspectives for It
Abstract: Rheumatoid arthritis (RA) is a chronic autoimmune disorder characterized by systemic inflammation, primarily affecting synovial joints, leading to joint destruction and systemic complications. The pathogenesis involves immune system dysregulation, autoantibody production, and enzymatic imbalances. Enzymes, including matrix metalloproteinases (MMPs), serine proteases, and kinases, play crucial roles in these processes. Their dysregulation contributes significantly to disease progression through the imbalance of pro-inflammatory cytokines, oxidative stress, and extracellular matrix (ECM) degradation. MMPs, …
Published in Research and Reviews : A Journal of Biotechnology · Vol. 15, Issue 2, 2025 · pp. 20–28 Read article