Research & Reviews : Journal of Food Science & Technology Review Article

A Review on Microbial Enzymes in the Food Industry: Current Innovations and Future Prospects

  1. Rajesh Kumar Rapture Biotech International (P) Ltd., Noida
  2. Chanchal Rapture Biotech International (P) Ltd., Noida
  3. Baanipreet Kaur Rapture Biotech International (P) Ltd., Noida
  4. Ruby Deshwal Rapture Biotech International (P) Ltd., Noida
  5. Ghazal Firoz Rapture Biotech International (P) Ltd., Noida
  6. Anjali Bharti Rapture Biotech International (P) Ltd., Noida
  7. Vinod Kumar Gupta Rapture Biotech International (P) Ltd., Noida

Abstract

Over the past few decades, microbial enzymes have become indispensable biocatalysts in food production, fundamentally revolutionizing how we approach manufacturing processes while simultaneously enhancing the quality of what we produce. Today, approximately 200 of the 4,000 known enzymes are deployed commercially with proteases, amylases, lipases, and lactases leading the charge in industrial food applications. This review brings together the latest innovations in how we use microbial enzymes for food processing, while also exploring exciting possibilities for their future. These enzymes do more than just improve texture and flavor, they extend shelf life, minimize waste, and enable the creation of genuinely sustainable food products. What makes this particularly exciting is how enzyme engineering, genetic modification, and precision fermentation have opened entirely new possibilities for customizing enzyme properties to meet specific industrial needs. The global food enzymes market continues to experience remarkable growth, fueled by what consumers increasingly demand: natural processing aids, products with clean labels, and manufacturing approaches that respect environmental constraints. But the field still faces real hurdles like regulatory compliance, maintaining enzyme stability under challenging industrial conditions and the persistent challenge of production costs all remain significant obstacles. Throughout this review, we examine where microbial enzymes make the biggest impact across dairy, bakery, beverages, meat processing, and specialty food sectors, while also addressing regulatory frameworks and charting out where enzyme technology development might head next.

Keywords

References (41)

  1. Fedoroff NV. Food in a future of 10 billion. Agriculture & Food Security. 2015;4(1). doi:10.1186/s40066-015-0031-7
  2. Bell EL, Finnigan W, France SP, Green AP, Hayes MA, Hepworth LJ, et al. Biocatalysis. Nature Reviews Methods Primers. 2021;1(1). doi:10.1038/s43586-021-00044-z
  3. Siddiqui SA, Erol Z, Rugji J, Taşçı F, Kahraman HA, Toppi V, et al. An overview of fermentation in the food industry - looking back from a new perspective. Bioresources and Bioprocessing. 2023;10(1). doi:10.1186/s40643-023-00702-y
  4. Singh R, Kumar M, Mittal A, Mehta PK. Microbial enzymes: industrial progress in 21st century. 3 Biotech. 2016;6(2). doi:10.1007/s13205-016-0485-8
  5. Mascarin GM, Golo PS, de Souza Ribeiro-Silva C, Muniz ER, de Oliveira Franco A, Kobori NN, et al. Advances in submerged liquid fermentation and formulation of entomopathogenic fungi. Applied Microbiology and Biotechnology. 2024;108(1). doi:10.1007/s00253-024-13287-z
  6. Boukid F, Ganeshan S, Wang Y, Tülbek MÇ, Nickerson MT. Bioengineered Enzymes and Precision Fermentation in the Food Industry. International Journal of Molecular Sciences. 2023;24(12):10156. doi:10.3390/ijms241210156
  7. Olempska-Beer ZS, Merker RI, Ditto MD, DiNovi MJ. Food-processing enzymes from recombinant microorganisms—a review. Regulatory Toxicology and Pharmacology. 2006;45(2):144-158. doi:10.1016/j.yrtph.2006.05.001
  8. Rebholz GF, Sebald K, Dirndorfer S, Dawid C, Hofmann T, Scherf KA. Impact of exogenous α-amylases on sugar formation in straight dough wheat bread. European Food Research and Technology. 2020;247(3):695-706. doi:10.1007/s00217-020-03657-y
  9. Espinosa-Ramírez J, Pérez-Carrillo E, Serna-Saldívar SO. Maltose and glucose utilization during fermentation of barley and sorghum lager beers as affected by β-amylase or amyloglucosidase addition. Journal of Cereal Science. 2014;60(3):602-609. doi:10.1016/j.jcs.2014.07.008
  10. Zhao X, Zheng Z, Zhang J, Sarwar A, Aziz T, Yang Z. Change of proteolysis and sensory profile during ripening of Cheddar‐style cheese as influenced by a microbial rennet from rice wine. Food Science & Nutrition. 2019;7(4):1540-1550. doi:10.1002/fsn3.1003
  11. Madhusankha GDMP, Thilakarathna RCN. Meat tenderization mechanism and the impact of plant exogenous proteases: A review. Arabian Journal of Chemistry. 2021;14(2):102967. doi:10.1016/j.arabjc.2020.102967
  12. Gu M, Hong T, Ma Y, Xi J, Zhao Q, Xu D, et al. Effects of a commercial peptidase on rheology, microstructure, gluten properties of wheat dough and bread quality. LWT. 2022;160:113266. doi:10.1016/j.lwt.2022.113266
  13. McSweeney PLH, Sousa MJ. Biochemical pathways for the production of flavour compounds in cheeses during ripening: A review. Le Lait. 2000;80(3):293-324. doi:10.1051/lait:2000127
  14. Reyes-Reyes AL, Valero Barranco F, Sandoval G. Recent Advances in Lipases and Their Applications in the Food and Nutraceutical Industry. Catalysts. 2022;12(9):960. doi:10.3390/catal12090960
  15. Sivakanthan S, Madhujith T. Current trends in applications of enzymatic interesterification of fats and oils: A review. LWT. 2020;132:109880. doi:10.1016/j.lwt.2020.109880
  16. Bendelja Ljoljić D, Dolenčić Špehar I, Kos I, Hulak N, Vnučec I. LACTOSE-FREE DAIRY PRODUCTS: A GROWING MARKET AND NUTRITIONAL ADVANTAGES. SBORNÍK L. KONFERENCE O JAKOSTI POTRAVIN A POTRAVINOVÝCH SUROVIN / BOOK OF THE 50th FOOD QUALITY AND SAFETY CONFERENCE. 2024:41-45. doi:10.11118/978-80-7509-996-9-0041
  17. Ribeiro DS, Henrique SMB, Oliveira LS, Macedo GA, Fleuri LF. Enzymes in juice processing: a review. International Journal of Food Science & Technology. 2010;45(4):635-641. doi:10.1111/j.1365-2621.2010.02177.x
  18. Liu X, Wang C, Zhang X, Zhang G, Zhou J, Chen J. Application Prospect of Protein-Glutaminase in the Development of Plant-Based Protein Foods. Foods. 2022;11(3):440. doi:10.3390/foods11030440
  19. Li L, Pei Y, Cheng K, Deng Y, Dong X, Fang R, et al. Production and evaluation of enzyme-modified cheese adding protease or lipase to improve quality properties. Journal of Bioscience and Bioengineering. 2023;135(5):389-394. doi:10.1016/j.jbiosc.2023.02.006
  20. Marcellino N, Beuvier E, Grappin R, Guéguen M, Benson DR. Diversity of Geotrichum candidum Strains Isolated from Traditional Cheesemaking Fabrications in France. Applied and Environmental Microbiology. 2001;67(10):4752-4759. doi:10.1128/aem.67.10.4752-4759.2001
  21. Yin L, Wei Y, Wu W, Peng Y, Ding W, Xiao S, et al. Effect of enzymes on baking quality of wheat dough and storage quality of toast bread. International Journal of Food Science and Technology. 2024;59(12):9479-9489. doi:10.1111/ijfs.17597
  22. Decamps K, Joye IJ, Courtin CM, Delcour JA. Glucose and pyranose oxidase improve bread dough stability. Journal of Cereal Science. 2012;55(3):380-384. doi:10.1016/j.jcs.2012.01.007
  23. Evans E. Brewing: Science and practice. J Sci Food Agric. 2005;86:169. doi:10.1002/jsfa.2344.
  24. Stanek-Wandzel N, Krzyszowska A, Zarębska M, Gębura K, Wasilewski T, Hordyjewicz-Baran Z, et al. Evaluation of Cellulase, Pectinase, and Hemicellulase Effectiveness in Extraction of Phenolic Compounds from Grape Pomace. International Journal of Molecular Sciences. 2024;25(24):13538. doi:10.3390/ijms252413538
  25. Hailemichael F. Production and Industrial Application of Microbial Aspartic Protease: A Review. International Journal of Food Engineering and Technology. 2021;5(2):85. doi:10.11648/j.ijfet.20210502.17
  26. González-Osuna MF, Bernal-Mercado AT, Wong-Corral FJ, Ezquerra-Brauer JM, Soto-Valdez H, Castillo A, et al. Bioactive Peptides and Protein Hydrolysates Used in Meat and Meat Products’ Preservation─A Review. ACS Food Science & Technology. 2024;4(5):1003-1016. doi:10.1021/acsfoodscitech.3c00605
  27. Fan Y, Zheng S, Annamalai PK, Bhandari B, Prakash S. Enhancement of the texture and microstructure of faba bean-based meat analogues with brewers' spent grain through enzymatic treatments. Sustainable Food Technology. 2024;2(3):826-836. doi:10.1039/d4fb00045e
  28. Ye H, Tao X, Zhang W, Chen Y, Yu Q, Xie J. Food-derived bioactive peptides: production, biological activities, opportunities and challenges. Journal of Future Foods. 2022;2(4):294-306. doi:10.1016/j.jfutfo.2022.08.002
  29. Porter JL, Rusli RA, Ollis DL. Directed Evolution of Enzymes for Industrial Biocatalysis. ChemBioChem. 2015;17(3):197-203. doi:10.1002/cbic.201500280
  30. Hu Z, Liu Y, Huang Y, Yu P. Advances in the Directed Evolution of Computer-aided Enzymes. Current Topics in Medicinal Chemistry. 2026;26(2):180-197. doi:10.2174/0115680266377310250526045137
  31. Deckers M, Deforce D, Fraiture MA, Roosens NHC. Genetically Modified Micro-Organisms for Industrial Food Enzyme Production: An Overview. Foods. 2020;9(3):326. doi:10.3390/foods9030326
  32. Huang W, Wang S, Wei Y, Bai Y, Zhu Z, Yin D, et al. Design and evolution of artificial enzyme with in-situ biosynthesized non-canonical amino acid. Nature Communications. 2025;16(1). doi:10.1038/s41467-025-63733-3
  33. Hu J, Arantes V, Pribowo A, Saddler JN. The synergistic action of accessory enzymes enhances the hydrolytic potential of a “cellulase mixture” but is highly substrate specific. Biotechnology for Biofuels. 2013;6(1). doi:10.1186/1754-6834-6-112
  34. Center for Food Safety and Applied Nutrition. Guidance for industry: Recommendations for submission of chemical and technological data for food additive petitions and GRAS notices for enzyme preparations. Silver Spring (MD): US Food and Drug Administration; 2022. Available from: http://www.fda.gov/FoodGuidances
  35. Zhang Y, Geary T, Simpson BK. Genetically modified food enzymes: a review. Current Opinion in Food Science. 2019;25:14-18. doi:10.1016/j.cofs.2019.01.002
  36. Modarres HP, Mofrad MR, Sanati-Nezhad A. Protein thermostability engineering. RSC Advances. 2016;6(116):115252-115270. doi:10.1039/c6ra16992a
  37. Du YH, Wang MY, Yang LH, Tong LL, Guo DS, Ji XJ. Optimization and Scale-Up of Fermentation Processes Driven by Models. Bioengineering. 2022;9(9):473. doi:10.3390/bioengineering9090473
  38. Hwang H, Nam SJ. The influence of consumers’ knowledge on their responses to genetically modified foods. GM Crops & Food. 2020;12(1):146-157. doi:10.1080/21645698.2020.1840911
  39. Søndergaard HA, Grunert KG, Scholderer J. Consumer attitudes to enzymes in food production. Trends in Food Science & Technology. 2005;16(10):466-474. doi:10.1016/j.tifs.2005.06.003
  40. Khan MF. Recent Advances in Microbial Enzyme Applications for Sustainable Textile Processing and Waste Management. Sci. 2025;7(2):46. doi:10.3390/sci7020046
  41. Prithviraj V, Díaz LP, Lemus-Mondaca R, Ullah A, Roopesh MS. Emerging advancements in 3D food printing. Frontiers in Food Science and Technology. 2025;5. doi:10.3389/frfst.2025.1607449
Support