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16 articles for “HPTLC Methods”
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HPTLC Method Development and Validation for Rabeprazole Sodium in Pharmaceutical Formulation
Abstract: Background: The most common medication for acid related disorders stands as Proton pump inhibitor (PPI) Rabeprazole Sodium. The pharmaceutical formulations require essential quality control of Rabeprazole Sodium through reliable analytical methods to maintain reliability. HPTLC provides a cost-effective rapid method for drug quantification compared to conventional HPLC methods so how HPTLC stands as an appropriate method for drug determination. Objective: The emphasis of this research project involves developing and validating …
Published in Research and Reviews: A Journal of Pharmaceutical Science · Vol. 16, Issue 2, 2025 · pp. 18–24 Read article
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Optimization of Mobile Phase and Stability—Indicating HPTLC Method for the Determination of Prasugrel Hydrochloride in Pharmaceutical Dosage Form
Abstract: A simple and sensitive high-performance thin layer chromatographic method was developed and validated for Prasugrel hydrochloride. It was spotted on silica gel 60F254 TLC plates, developed using toluene:methanol (9.9:0.1, v/v) as mobile phase and scanned at 258 nm using a Camag TLC Scanner IV. The Rf value of Prasugrel hydrochloride were found to be 0.53±0.03. It was found to be linear in the concentration range of 300–1500 ng/band. The limit …
Published in Research & Reviews: A Journal of Drug Formulation, Development and Production · Vol. 5, Issue 2, 2018 · pp. 7–12 Read article
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HPTLC Method Development and Validation of Genistein and Daidzein Estimation in Mucuna cochinchinensis Seed Extracts
Abstract: Aim: The current study was designed with the objective to estimate the concentration of Genistein and Daidzein in ethyl acetate extract of Mucuna cochinchinensis by high-performance thin-layer chromatography (HPTLC).Methods: The separation of bioactive compounds was performed using mobile phase, Toluene: n-Butanol: Glacial Acetic Acid: Water (1:4:2:2) and detected at wavelength 254 nm. The method was validated for linearity, accuracy, precision, limit of detection (LOD) and limit of quantification (LOQ) by …
Published in Research & Reviews: A Journal of Drug Formulation, Development and Production · Vol. 7, Issue 1, 2020 · pp. 16–25 Read article
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Development and Validation of HPTLC Methods for Estimation of Trimebutine Maleate in Pharmaceutical Formulation
Abstract: A simple and rapid high-performance thin-layer chromatographic (HPTLC) method was developed and validated for quantitative determination of trimebutine maleate in single dose formulation. Trimebutine maleate was chromatographed on silica gel 60 F 254 TLC plate using methanol–toluene (8:2 v/v) as mobile phase. The plates were developed to a distance of 8 cm at ambient temperature. Trimebutine maleate was quantified by densitometric analysis at 271 nm. The method was found to …
Published in Research and Reviews: A Journal of Pharmaceutical Science · Vol. 8, Issue 1, 2017 · pp. 17–22 Read article
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A Developed and Validated Densitometric HPTLC method for Quantitative Analysis of Cefdinir
Abstract: A sensitive and accurate high performance thin layer chromatographic method has been developed and validated. Chromatographic separation was performed on silica gel F254 HPTLC plates, prewashed with methanol using methanol: toluene (9.5: 0.5 v/v), as solvent system. The plates were scanned densitometrically, with a deuterium lamp at 286 nm. The method was validated in accordance with the requirements of the ICH guidelines. The response to cefdinir concentration was linear in …
Published in Research and Reviews: A Journal of Pharmaceutical Science · Vol. 1, Issue 1, 2010 Read article
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Advancements in Analytical Techniques and Validation Methods for Pharmaceutical Tablet Formulations Using HPTLC
Abstract: High Performance Thin Layer Chromatography (HPTLC) is a versatile analytical technique which has been effectively used for pharmaceutical quality control and cost effectively, rapidly, and high throughput analyzed tablet formulations. HPTLC is different from traditional chromatographic methods as multi sample analysis is possible in one run with low consumption of solvent, which are principles of green analytical chemistry. But existing HPTLC is limited in sensitivity, resolution and reproducibility and these …
Published in Research & Reviews: A Journal of Drug Formulation, Development and Production · Vol. 13, Issue 3, 2026 Read article
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Validation of Wet and Dry Drug Collection Principle in the Preparation of Shatavari Ghrita through Quantitative Estimation of Shatavarin IV
Abstract: Background: Ayurved classics accentuates wet and dry drug collection principle i.e. wet state of drugs should be taken in double quantity to the quantity of dry drugs during the preparation of any formulation. But some Acharya has given the exceptional drugs for this principle where those drugs should be taken always in wet state with mentioned quantity. Shatavari is one of exceptional drug for this principle. Aim: The present study …
Published in Research & Reviews: A Journal of Drug Formulation, Development and Production · Vol. 8, Issue 2, 2023 · pp. 28–41 Read article
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HPTLC: An Emerging Technique in Herbal Medicine
Abstract: Herbal medicine and their formulations are extensively used since time immemorial in developing and well developed countries. Their quality assessment is of benefit not only in research but also in practice. However, quality control of these herbo-mineral preparations is more difficult than that of conventional drug due to chemical complexity and natural variability of product and raw materials. Furthermore, the active principles are, in most cases unknown and selective analytical …
Published in Research & Reviews : Journal of Herbal Science · Vol. 4, Issue 2, 2015 · pp. 25–37 Read article
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Isolation and Characterization of β -D-Glucan from Yeast and its In Vitro Biological Study
Abstract: In many microorganisms, fungi and algae, β-D-glucan can be found as cell wall polysaccharide. Various publications describe the strong positive influence of these glucans on the immune system comprising antibacterial, woundhealing and antitumor activities. Recently, it was found that β-D-glucan from the yeast Saccharomyces cervisiae also exhibit antioxidative capabilities. In the present study, the β-D-glucan was isolated from the cell wall of yeast and it is characterized by chromatographic, spectroscopic …
Published in Research and Reviews : A Journal of Biotechnology · Vol. 2, Issue 1, 2012 Read article
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Analytical Method Development for Quality Control and Standardization of Medicinal Plants: A Critical Review
Abstract: The demand for herbal medicine is increasing as herbs are less toxic, affordable, and culturally acceptable than the conventional drugs in developing countries. Thus, the increase in demand for standardization to ensure safety, purity and quality of medicinal plant. Standardization is a process of confirming herbal medicine identity and determination of its quality and purity. It involves botanical, physical, chemical and biological evaluations. These require application of different analytical methods …
Published in Research & Reviews : Journal of Herbal Science · Vol. 10, Issue 1, 2021 · pp. 9–20 Read article
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Analytical and in Vitro Evaluation of Krimighna Karma of Padumale, A Folklore Plant–Lindernia Crustacea (L) F. Muell W.S.R. To Diarrheagenic Organisms
Abstract: Traditional knowledge of medicinal plants got more importance in recent era. Hence there is a need to develop new effective traditional drugs with scientific validation. One such plant is Lindernia crustacea (L) F. Muell called as padumale in south canara district, Karnataka. It is small slender prostrate plant found in moist shady places. The local people successfully use the juice of fresh plant in few drops once in a day …
Published in Research and Reviews : A Journal of Ayurvedic Science, Yoga and Naturopathy · Vol. 9, Issue 03, 2022 · pp. 1–11 Read article
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Bergenin: Review of The Methods of Determination and Quantification
Abstract: Studies have shown that Bergenin has various pharmacological functions, such as Anti-lithiatic, Anti-oxidant, anti-inflammatory, Anti-bacterial, Anti-viral, Anti-cancer, Anti-cholinesterase, Anti-diabetic, Hypolipidemic, Anti-tussive, Hepatoprotective and Immunomodulatory. Bergenin is found in the Saxifragaceae, Euphorbiaceae, Myrsinaceae, Dipterocarpaceae, and Fabaceae genera and a range of beneficial effects on human health. The number of bioactive compounds in various bergenia species must be determined in order to achieve the best quality raw material for herbal preparations. As …
Published in Research & Reviews: A Journal of Drug Formulation, Development and Production · Vol. 8, Issue 2, 2023 · pp. 52–66 Read article
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HPTLC: An Important Tool for Estimation of Phytoconstituents
Abstract: AbstractHPTLC (High-performance thin layer chromatography) technique is widely employed in pharmaceutical industry for the process development, identification and estimation of adulterants in herbal formulations and helps in estimation and identification of pesticide content, mycotoxins and in quality control of nutraceuticals. For the herbal extracts and especially for the botanical drugs, the issue of stability is of exceptional importance. Standardization is required for the development of an accurate biological activity and …
Published in Research and Reviews: A Journal of Pharmaceutical Science · Vol. 7, Issue 1, 2016 · pp. 28–33 Read article
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Antibacterial, Cytotoxicity Studies of Azukia mungo (L.) Masam and Correlation with in silico Docking Studies
Abstract: Introduction and Background: Azukia genus plants rich in proteinaceous anti-nutrients like tannins (especially condensed tannins) has been shown to have anti-bacterial activity against Staphylococcus aureus and antineoplastic activity against lung and liver cancer cells. Five types of procyanidins (condensed tannins) have already been isolated from Azukia mungo and structurally elucidated. But its activity against the Methicillin resistant Staphylococcus aureus (MRSA) has not yet been shown. Hence we focus our work …
Published in Research and Reviews: A Journal of Toxicology · Vol. 7, Issue 2, 2017 · pp. 21–31 Read article
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Pharmacognostical and Analytical Profile of Vasa Ghrita
Abstract: Vasa Ghrita is an Ayurvedic formulation, which is beneficial in the management of Raktapitta, Shawasa, Kasa and Jirna Jwara. Vasa Ghrita was prepared as per method mentioned in Charaka Samhita. A pharmacognostical study involving powder microscopy of raw drugs of Vasa plant and Flower. Organoleptic and Physicochemical analysis of the finished product was carried out, to evaluate the quality of the formulation. The specific gravity of the formulation was 1.4543 …
Published in Research & Reviews: A Journal of Pharmacognosy · Vol. 8, Issue 3, 2023 · pp. 38–43 Read article
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A Comparative Analytical Study on Gandhaka Ointment Prepared by Two Different Oleaginous Bases – As a Thesis Article
Abstract: Gandhaka (purified sulphur) is broadly used in Ayurvedic dermatological preparations, particularly in Malahara Kalpana (ointment formulations), due to its well-known antimicrobial and skin-healing properties. Although classical Ayurvedic texts, such as Rasa Shastra and Bhaishajya Kalpana, do not directly mention Gandhaka ointment, Rasatarangini by Acharya Sadanand Sharma describes four discrete methods for preparing Gandhaka Taila (sulphur-infused oil). In this study, the third method was selected to prepare Gandhaka Taila using two …
Published in Journal of AYUSH: Ayurveda, Yoga, Unani, Siddha and Homeopathy · Vol. 14, Issue 3, 2025 · pp. 27–39 Read article