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8 articles for “Agarose”
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A Rapid and Efficient Protocol of DNA Extraction from Animal Blood and Plant Parts
Abstract: DNA extraction from plant tissues is much more complex in comparison to mammalian tissues and bacterial cells due to the presence of a rigid cell wall. Methods utilized for extraction of DNA from plant tissues can vary depending upon the materials used. It is very essential to obtain the nuclear material (without degradation) by breaking down the cell wall and membrane mechanically. We have used the single protocol for extraction …
Published in Research and Reviews : A Journal of Biotechnology · Vol. 3, Issue 3, 2013 · pp. 1–4 Read article
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Application of pET28a Vector for the Over Expression of Recombinant Human Tim23 Protein in E. coli and its Validation by Immunoblotting
Abstract: This study aimed to produce recombinant human Tim23 protein by molecular cloning, expression, and purification. Amplification of human TIM23 was carried out by polymerase chain reaction (PCR) and cloned into DH5α cells. The pET28a-human TIM23 was transformed into BL21DE3 and expressed the gene by IPTG induction. Recombinant pure human Tim23 antigen was prepared, and injected into rabbits and polyclonal antibodies were raised, which was confirmed by western blotting. The transformed …
Published in Research and Reviews : A Journal of Life Sciences · Vol. 14, Issue 1, 2024 · pp. 32–44 Read article
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Polysaccharide-Based Hydrogels for Sustained Drug Release in Pharmacology
Abstract: Polysaccharide-based hydrogels have showed great potential in medicine as materials for long-lasting, under control medication delivery systems. Natural polysaccharides include chitosan; alginate, agarose, and cellulose form these hydrogels. Among its many advantages are biocompatibility, biodegradability, and customising ability for various drug release rates. Reacting to pH, temperature, and ionic strength changes in the surroundings, polysaccharide-based hydrogels may swell. For usage requiring the release of therapeutic substances over an extended period …
Published in Journal of Polymer & Composites · Vol. 13, Issue 4, 2025 · pp. 300–312 Read article
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Cadmium Accumulation in Different Tissues and DNA Content Alteration in Cat Fish (Clarias batrachus) Exposed to Lethal Concentration of Cadmium
Abstract: In the present study, the acute toxicity experiment was conducted to evaluate LC50 of Cadmium chloride (CdCl2) on Clarias batrachus which was found to be 450 ppm for 96 h. Clarias batrachus (average length of 8.5 ± 0.4 cm and the average weight of 13.5 ± 0.393 g) was exposed to sub-lethal concentration (140 ppm) and lethal (450 ppm) concentrations of Cd for 14 days. The Cd accumulation in tissues …
Published in Research and Reviews: A Journal of Toxicology · Vol. 10, Issue 3, 2020 · pp. 1–8 Read article
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A Duplex PCR for Simultaneous Detection of Brucella and Bovine Herpesvirus – Genome in Clinical Samples
Abstract: Normal 0 false false false EN-US X-NONE X-NONE MicrosoftInternetExplorer4 A disease-free germplasm is a prerequisite for getting healthy progeny. Many diseases are transmitted through semen either by natural service or artificial insemination causing reproduction failure and economic losses. The intermittent excretion of Bovine Herpesvirus-1 (BoHV-1) and Brucella in semen and their transmission through artificial insemination has been recorded. PCR has become an invaluable tool because of speed and simplicity with …
Published in Research and Reviews: A Journal of Microbiology and Virology · Vol. 1, Issue 2, 2011 · pp. 1–7 Read article
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Existence of Integron I and II Genes from Clinical Isolate Staphylococcus aureus and Their Role in Drug Resistance
Abstract: An integron is a gene capture and dissemination system. They have been identified on both genomic and mobile genetic elements which often contain one or more genes that encode antibiotic resistance. They are commonly associated with bacterial genera in the family of Enterobacteriaceae. Currently, there have been recent reports showing the presence of integrons in Staphylococcus species. The aim of the present study was to assess the existanxe of Class …
Published in Research and Reviews: A Journal of Microbiology and Virology · Vol. 4, Issue 3, 2014 · pp. 18–24 Read article
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Semi-quantitative Event Specific Detection of Bt Maize (Event MON 810) using Real-Time PCR with SYBR Green Ι Chemistry
Abstract: The objective is this work is to do semi-quantitative analysis for Bt maize (event MON 810) using conventional Polymerase Chain Reaction (PCR) and Real Time PCR (SYBR green chemistry) from the fresh maize samples chosen arbitrarily from various locations. The protocol for extraction of good quality genomic Deoxyribonucleic acid (DNA) was standardized without the use of Liquid nitrogen. The DNA used for further studies were obtained by using standardized protocol. …
Published in Research and Reviews : A Journal of Biotechnology · Vol. 2, Issue 3, 2012 · pp. 25–34 Read article
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Review on Advanced Molecular Diagnostic Tools and Their Applications
Abstract: Although the diagnosis of diseases is still primarily based on conventional methods (staining, culture, biochemical analysis), a series of advanced molecular diagnostic tools are increasingly introduced and incorporated in the workflow of clinical laboratories worldwide. These tools, such as polymerase chain reaction, northern blotting, restriction length polymorphism, flow cytometry, southern blotting, fluorescent in situ hybridization, agarose gel electrophoresis and pyrosequencing that are based on either the properties of nucleic acids …
Published in Research and Reviews : A Journal of Life Sciences · Vol. 3, Issue 3, 2013 · pp. 21–33 Read article