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247 articles for “assay”
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Phyto-bioefficiency of Vijaya Extract for Normal Cell Protection and Its Anticancer Efficiency Against Breast Cancer Xenograft
Abstract: Cannabis is an annual, dioecious, flowering herb. Its leaves are palmately compound by serrate leaflets. Cannabis sativa maximum phytochemicals are soluble in organic solvent. The Cannabis plant has been historically used due to its medicinal properties since thousands of years through several beliefs. In India, the department of narcotics has sanction that Cannabis can be grown in a monitored way with proper approval and license. The present study concludes that …
Published in Research and Reviews: Journal of Oncology and Hematology · Vol. 11, Issue 3, 2022 · pp. 15–21 Read article
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Antibacterial, Cytotoxicity Studies of Azukia mungo (L.) Masam and Correlation with in silico Docking Studies
Abstract: Introduction and Background: Azukia genus plants rich in proteinaceous anti-nutrients like tannins (especially condensed tannins) has been shown to have anti-bacterial activity against Staphylococcus aureus and antineoplastic activity against lung and liver cancer cells. Five types of procyanidins (condensed tannins) have already been isolated from Azukia mungo and structurally elucidated. But its activity against the Methicillin resistant Staphylococcus aureus (MRSA) has not yet been shown. Hence we focus our work …
Published in Research and Reviews: A Journal of Toxicology · Vol. 7, Issue 2, 2017 · pp. 21–31 Read article
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Cyto-toxicity of Silver Nanoparticles on A Bacterial Population Isolated From Lakewater
Abstract: Due to the increased industrial and consumer applications, there is growing concern regarding the possible risk associated with their environmental exposure. Silver nanoparticles are considered to be toxic both to environment and human beings after a permissible limit. The aim of the present study was to explore the difference in toxicity mechanism of Silver nanoparticles (NPs) at low concentrations (≤1 μg/mL), in a lake-water bacterial consortia containing Bacillus marisflavi and …
Published in Research and Reviews: A Journal of Toxicology · Vol. 4, Issue 1, 2014 · pp. 22–25 Read article
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The Effect of Vitamin C on the Reproductive Functions of Paraquat Intoxicated Male Albino Rats (Rattus norvegicus)
Abstract: Paraquat (PQ) is a bipyridylium herbicide applied around trees in Orchids and between crop rows to control broad-leaved and grassy weeds. Its oxidation results in the formation of superoxides and supernitrite species which causes damage to cellular components. Studies have implicated PQ as one of the pesticides that contributes to the reduction in male reproductive functions in humans and animals during exposure. This study, therefore, determined the antioxidant effect vitamin …
Published in Research and Reviews: A Journal of Toxicology · Vol. 4, Issue 3, 2014 · pp. 9–29 Read article
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Arsenic Induced Cytotoxicity Study Against Human Epithelial Carcinoma (HeLa) Cell
Abstract: The present investigation was undertaken to find out the cytotoxic effects of various amounts of arsenic exposure to human epithelial carcinoma (HeLa) cell. Cells were treated with a final concentration (0, 10, 20, 30, 40 and 50 µg/l) of arsenite in 5 ml MEM for 24 h where 0 concentration was considered as control. Cell viability was determined by the trypan blue (TB) exclusion test using hematocytometer and automated BIO-RAD …
Published in Research and Reviews: A Journal of Toxicology · Vol. 5, Issue 1, 2015 · pp. 1–8 Read article
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Review on Histological Effect of Lead on Liver and Kidney
Abstract: Our environment can be polluted by environmental toxins which are chemicals and other materials produced largely from industry and its products. There are large numbers of environmental toxicants which are potentially harmful to health. These large numbers of environmental toxicants can be divided into three major categories (heavy metals, air pollutants, and pesticides) and they are commonly studied environmental toxicants. Environmental toxins can be entered into the body by various …
Published in Research and Reviews: A Journal of Toxicology · Vol. 10, Issue 1, 2020 · pp. 8–29 Read article
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Recent Advances in Nanotechnology-Assisted Liquid Crystal Biosensors for Sensitive and Naked-Eye Detection of Antibiotics
Abstract: The widespread use of antibiotics in healthcare, veterinary medicine, agriculture, and aquaculture has resulted in increasing levels of antibiotic residues in food products and environmental samples, posing significant risks to human health and contributing to antimicrobial resistance. Conventional analytical methods, including high-performance liquid chromatography (HPLC), liquid chromatography–mass spectrometry (LC–MS), enzyme-linked immunosorbent assay (ELISA), and electrochemical techniques, offer excellent sensitivity but require sophisticated instrumentation, skilled personnel, and extensive sample preparation. In …
Published in International Journal of Crystalline Materials · Vol. 3, Issue 2, 2026 Read article
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Biosynthesis, Characterization and Non-toxic Activity of Gold Nanoparticles using Geranium (Pelargonium graveolens L.) Leaf Extract
Abstract: Nanoparticles from various materials can be prepared by relatively simple methods. During the past few decades, a variety of different methods have been reported and reviewed for synthesizing gold colloids of monodisperse and uniform sized particles. In present investigation, a new method of one-pot biosynthesizing of gold nanoparticles (GNPs), using geranium leaves extract as a reductant and stabilizer is reported. GNPs were characterized by UV-visible spectroscopy; transmission electron microscopy, X-ray …
Published in Nano Trends – A Journal of Nano Technology & Its Applications · Vol. 16, Issue 3, 2014 · pp. 1–7 Read article
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Gold Nanoparticles: Preparation, Characterization and Its Stability in Buffer
Abstract: Among the colorimetric detection tactics, metallic nanoparticle based detection is gaining more attention due to quickness and less resource requirement at the point of use. Unique chemical inertness, shape, size and optical attributes of gold nanoparticles (GNPs) make them ideal for various diagnostic tools. In this approach synthesized GNPs were stabilized by citrate counter ions. These GNPs were characterized for their optical properties, shape, size, zeta potential, and dynamic light …
Published in Nano Trends – A Journal of Nano Technology & Its Applications · Vol. 17, Issue 1, 2015 · pp. 1–10 Read article
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Novel Modified Chitosan/Clay Nanocomposites with Antimicrobial Activity
Abstract: Unmodified sodium montmorillonite (Na-MMT) and montmorillonite modified by poly(methylmethacrylate) (PMMA) were used to intercalate chitosan (CS) and chitosan blended with polytertahydrofuran (CS-PTHF750). Their structures were characterized by XRD, TEM and IR techniques. The results showed that CS and CS-PTHF750 chains were inserted into silicate layers. The interlayer distance of the layered silicates in the nanocomposites enlarged as the amount of polymer increased. The thermal stability of the nanocomposites is characterized …
Published in Journal of Modern Chemistry & Chemical Technology · Vol. 4, Issue 2, 2013 · pp. 8–18 Read article
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Antimicrobial Activity of Alcoholic and Aqueous Extracts of Tinospora cordifolia
Abstract: 1024x768 Normal 0 false false false EN-US X-NONE X-NONE /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Table Normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-priority:99; mso-style-qformat:yes; mso-style-parent:""; mso-padding-alt:0in 5.4pt 0in 5.4pt; mso-para-margin:0in; mso-para-margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:11.0pt; font-family:"Calibri","sans-serif"; mso-ascii-font-family:Calibri; mso-ascii-theme-font:minor-latin; mso-fareast-font-family:"Times New Roman"; mso-fareast-theme-font:minor-fareast; mso-hansi-font-family:Calibri; mso-hansi-theme-font:minor-latin; mso-bidi-font-family:"Times New Roman"; mso-bidi-theme-font:minor-bidi;} The success of chemotherapy lies in the continuous search for new drugs to counter the challenge posed by resistant strains. Ethanolic and aqueous extracts of plant Tinospora …
Published in Research and Reviews: A Journal of Pharmaceutical Science · Vol. 2, Issue 1, 2011 · pp. 1–6 Read article
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Fabrication and Characterization of Sustained Release Beads of Carbamazepine by Extrusion – Spheronization Containing Carbopol®974
Abstract: The erratic absorption of Carbamazepine, BCS Class II drug, due to low water solubility and anticholinergic properties can be minimized by developing sustained release beads. Sustained release beads of Carbamazepine were prepared using axial single screw extruder and a chequered plate spheronizer. Beads offer therapeutic advantages such as easy dispersion in the gastro-intestinal tract, lower risk of dose dumping, reduce peak plasma fluctuations and drug release is independent of gastric …
Published in Research and Reviews: A Journal of Pharmaceutical Science · Vol. 2, Issue 2, 2011 · pp. 11–21 Read article
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Evaluation of Anti-ulcer Activity of Ethanolic Extract of Salacia fruticosa and Oldenlandia Umbellata
Abstract: In this present study the aerial and root parts of Salacia fruticosa and the entire herb of Oldenlandia umbellate were evaluated for its anti-ulcer property in Aspirin induced ulcer model and Pylorus ligation assays. Both plant materials were extracted with ethanol and given at the dose level of 200mg/kg orally after the LD50 studies to fix the dose. Ulcer index, free acidity and total acidity in pylorus ligated and aspirin …
Published in Research and Reviews: A Journal of Pharmaceutical Science · Vol. 4, Issue 2, 2013 · pp. 1–5 Read article
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Modified Nucleosides “(ProTide)†as Potential Anti-HCV Therapeutics
Abstract: Hepatitis C virus (HCV) is among the most common causes of cirrhosis and chronic liver disease worldwide. As a result, many researchers are interested in designing and synthesis of a clinical treatment for HCV. Nucleoside monophosphates and monophosphonates play such an important role for treatments of incurable diseases such as hepatitis. For example, 2'-C-methyladenosine and 2'-C-methyl guanosine have shown activities against HCV in the replicon assay as well as against …
Published in Research and Reviews: A Journal of Pharmaceutical Science · Vol. 8, Issue 1, 2017 · pp. 32–39 Read article
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Phytochemical constituents of some plants used by the tribals of Dakshin Dinajpur district for medicinal purposes
Abstract: Various phytochemical constituents including alkaloids, phenols, and ascorbic acids were assessed and compared in the four medicinal plants of different families. Four plants used locally for different medicinal purposes were selected for a comparison of their phytochemical constituents. The plants investigated were Clerodendrum viscosum, Moringa oleifera, Cinnamomum tamala and Scoparia dulcis. All theses plants were found to contain the active principles including alkaloids, tannin, saponin, terpenoid, flavonoid, cardic glycoside. Steroid …
Published in Research and Reviews: A Journal of Pharmacology · Vol. 1, Issue 1, 2011 · pp. 1–7 Read article
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In vitro Antioxidant Activity of Calotropis Procera
Abstract: Antioxidant activity of root of Calotropis procera extract was evaluated in a series of in vitro assay involving free radicals. IC50 values were determined. Extracts of I. coccinea exhibited scavenging effect in concentration dependent manner in all in vitro method. IC50 of ethyl acetate extract was 48.43, 75.811 and 60.60 μg/ml for 2,2-diphenylpicrylhydrazyl radical scavenging, hydrogen peroxide scavenging, and nitric oxide scavenging, respectively. Ethyl acetate extract shows good in vitro …
Published in Research and Reviews: A Journal of Pharmacology · Vol. 4, Issue 2, 2014 · pp. 11–14 Read article
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Phytochemical and Physico-chemical Standardization of Narayana Taila: Ayurvedic Medicated Oil for Arthritis
Abstract: Narayana Taila is an important formulation of Ayurveda system of medicine used to treat arthritis (Amavatha). The present study aimed to set quality parameters in physico-chemical, thin layer Chromatography for Narayana Taila, assays for physico-chemical analysis, phytochemical profile. The finding obtained from the study might be used as standard values for Narayana Taila. Keywords: Narayana Taila, Medicated oil, ArthritisCite this Article Madhumathi Sundararajan, Vasanth Kumar Mohan, Thangamani Pakkirisamy. Phytochemical and …
Published in Research and Reviews: A Journal of Pharmacology · Vol. 7, Issue 1, 2017 · pp. 19–24 Read article
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A Duplex PCR for Simultaneous Detection of Brucella and Bovine Herpesvirus – Genome in Clinical Samples
Abstract: Normal 0 false false false EN-US X-NONE X-NONE MicrosoftInternetExplorer4 A disease-free germplasm is a prerequisite for getting healthy progeny. Many diseases are transmitted through semen either by natural service or artificial insemination causing reproduction failure and economic losses. The intermittent excretion of Bovine Herpesvirus-1 (BoHV-1) and Brucella in semen and their transmission through artificial insemination has been recorded. PCR has become an invaluable tool because of speed and simplicity with …
Published in Research and Reviews: A Journal of Microbiology and Virology · Vol. 1, Issue 2, 2011 · pp. 1–7 Read article
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Isolation and Characterization of Protease Producing Organism Bacillus Species from Slaughter House Waste
Abstract: The purpose of this investigation was to isolate protease producing bacteria from slaughter house waste collected in local area of Kaliakkavilai. The Bacillus sp. was identified by several biochemical and physiological tests. The effect of different production parameters such as pH, temperature, metal ions concentration and substrate concentration for protease production by the isolated bacterial strain were studied. The enzyme production was assayed in submerged fermentation with optimized parameters to …
Published in Research and Reviews: A Journal of Microbiology and Virology · Vol. 3, Issue 3, 2013 · pp. 8–14 Read article
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Assessment of the Saliva and Serum Interleukine-6 in Patients with Chronic Periodontitis: Case Controlled Study
Abstract: Abstract:Chronic periodontitis is a multifactorial disease characterized by an interaction between dental plaque antigen and components of the host defense system. Cytokines are considered to play a key role in the inflammation process in chronic periodontitis. The aim of the study was to assess the levels of Interleukin-6(IL-6) in saliva and serum of patients with chronic periodontitis and to compare them with the levels that observed in healthy subjects. This …
Published in Research and Reviews: A Journal of Microbiology and Virology · Vol. 5, Issue 1, 2015 · pp. 1–7 Read article