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75 articles for “acetone”
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Production, Optimization and Purification of Laccase Produced by Pleurotus ostreatus MH591763
Abstract: The optimization of production medium for laccase produced by Pleurotus ostreatus (Gene Bank Accession number MH591763) to reduce the cost of laccase production in large scale is the basic research in industrial field. In this study, the laccase culture medium was optimized for growth parameters such as incubation period, pH, temperature, carbon and nitrogen source on fungal growth and enzyme production. Purification and characterization of the enzyme was done by …
Published in Research and Reviews: A Journal of Microbiology and Virology · Vol. 9, Issue 1, 2019 · pp. 56–64 Read article
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Growth Inhibitory Effects of Centella asiatica Extracts on Escherichia coli
Abstract: Plant secondary metabolites have been known for their varying degrees of antimicrobial activity. These form important constituent of traditional medicine. The present study aims to analyze the antibacterial effects of the Centella asiatica extracts by looking into the growth inhibitory effects of organic and aqueous extracts of the leaves on Escherichia coli bacterium and to identify the secondary metabolites present in the extract by phytochemical analysis. The leaf extracts of …
Published in Research and Reviews: A Journal of Microbiology and Virology · Vol. 9, Issue 2, 2019 · pp. 1–7 Read article
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HPLC and Tandem MS Device for the Recognition of Emtricitabine and Tenofovir Alafenamide in K2EDTA Human Plasma Utilizing Emtricitabine 15ND2 Tenofovir D5 as an IS
Abstract: Developed very impressionable, discriminating, and exact extreme-efficiency liquid chromatography-tandem bulk spectrometry form for measurement of Emtricitabine and Tenofovir Alafenamide in human plasma. Emtricitabine15ND2 Tenofovir D5 was used as a within the standard (IS). The samples were divided on Phenomenex Kinetex C18 5 µm 100A, 100*4.6 mm procession utilizing Acetonitrile: 0.2% formic acid, 60:40 V/V as movable state, the retention period of Emtricitabine: 1.36 minutes (±0.5 minutes), Emtricitabine 15ND2: 1.36 minutes …
Published in Research and Reviews: A Journal of Toxicology · Vol. 12, Issue 2, 2022 · pp. 30–38 Read article
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Parametric Analysis on Pulsating Heat Pipes
Abstract: Day-by-day the size of the electronic components is being drastically decreased, but at the same time the computing capacities are improved manifold. This presents a new challenge in the thermal management of these components. Pulsating Heat Pipes (PHP) can transport warmth for considerable distances leading to placement of condenser away from compact cabinets. From the literature, it is observed that Acetone, Ethanol and Distilled water are the most widely used …
Published in Recent Trends in Fluid Mechanics · Vol. 4, Issue 1, 2017 · pp. 38–43 Read article
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Development and Validation of Liquid Chromatography- Tandem Mass Spectrometric Method for Assay of Zidovudine in Human Urine
Abstract: A sensitive and selective liquid chromatographic-tandem mass spectrometric (LC-MSMS)method was developed to determine zidovudine (ZDV) in human urine. After solidphaseextraction with SPE cartridge, the urine sample was analyzed on a C18 column(Symmetry 3.5 μm; 75×4.6 mm i.d) interfaced with a triple quadrupole tandem massspectrometer. Positive electrospray ionization was employed as the ionization source. Themobile phase consisted of water-acetonitrile (20:80, v/v).The method was linear over aconcentration range of 10–1000 ng mL-1. …
Published in Research and Reviews: A Journal of Pharmaceutical Science · Vol. 5, Issue 3, 2014 · pp. 17–23 Read article
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Determination of Gatifloxacin in Human Urine by Liquid Chromatography-Tandem Mass Spectrometry
Abstract: Gatifloxacin (GTF) is an antibiotic of the fourth-generation fluoroquinolone family that inhibits the bacterial enzymes DNA gyrase and topoisomerase IV. A reliable, sensitive and selective isocratic reversed phase liquid chromatographic-tandem mass spectrometric (LC-MS-MS) method was developed to determine gatifloxacin (GTF) in human urine. GTF, in urine after solid-phase extraction with SPE cartridge, was analyzed on a C18 column (Symmetric 3.5 μm; 75×4.6 mm i.d) interfaced with a triple quadrupole tandem …
Published in Research and Reviews: A Journal of Pharmaceutical Science · Vol. 6, Issue 1, 2015 · pp. 1–6 Read article
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Stability Indicating HPLC Method for Drugs Indicated for Helicobacter Pylori-associated Ulcers
Abstract: Normal 0 false false false EN-US X-NONE AR-SA MicrosoftInternetExplorer4 Triple therapy regimen that comprises a proton pump inhibitor likes Omeprazole, antibiotics like Doxycycline and Tinidazole can be used for treatment of peptic ulceration which is caused by H. pylori. A validated HPLC method was developed as a stability-indicating HPLC method for these drugs and its impurities. New method overcame interference between Doxycycline and its epimer on achiral column by using …
Published in Research and Reviews: A Journal of Pharmaceutical Science · Vol. 6, Issue 1, 2015 · pp. 28–46 Read article
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Development and Validation of HPLC Method for Simultaneous Estimation of Cilnidipine and Valsartan in Bulk and Tablet Dosage Form
Abstract: A simple, sensitive, accurate and precise reverse phase high performance liquid chromatographic method has been developed for the simultaneous estimation of Valsartan and Cilnidipine. The purposed RP-HPLC method utilizes stationary phase and consists of symmetry C18 (250 × 4.6 mm, 5 μm in particle size) with a mobile phase comprising of Acetonitrile: Ammonium Acetate Buffer (75:25%v/v) pH 5.0 adjusted by Triethylamine at a flow rate of 1.0 mL/min, column temperature …
Published in Research and Reviews: A Journal of Pharmaceutical Science · Vol. 6, Issue 2, 2015 · pp. 28–36 Read article
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Isolation and Partial Characterization of a Lectin from Seeds of Pongamia Glabra
Abstract: Lectins are non-immunogenic proteins that selectively bind carbohydrates without processing them enzymatically.They have wide range of applications in agricultural and biomedical research and it is useful in the antidotal treatment of snake bite. Seed lectins can be attributed to the importance of legume seeds as a rich source of dietary protein. The Pongamia glabra were homogenized by using distilled acetone. The defatted dried seed meal was mixed with 20mm Phosphate …
Published in Research and Reviews : A Journal of Biotechnology · Vol. 1, Issue 1, 2011 · pp. 87–95 Read article
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Biotherapeutic Antibacterial Activity of Some Medicinal Plants Against Predominant Bacteria Causing Dental Caries
Abstract: Dental caries is a microbial disease of the calcified tissues of the teeth, characterized by demineralization of inorganic portion and destruction of the organic substance of the tooth. It is most prevalent chronic disease affecting the human race. The in vitro antibacterial activity of some medicinal plants was studied against pathogenic bacteria causing dental caries. Leaves were extracted using petroleum ether, chloroform, acetone, methanol and distilled water. Among the five …
Published in Research and Reviews : A Journal of Life Sciences · Vol. 2, Issue 1, 2012 · pp. 11–15 Read article
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Development of PLLA-based Bio Inks Applicable in the Medical Field
Abstract: Some synthetic polymers, such as Poly (L-lactic acid) (PLLA), are interesting to use in 3D bioprinting because they can have adaptable and resilient mechanical properties that also facilitate bioprinting processes. PLLA is a biocompatible polymer which makes it very interesting for medical applications, it is an aliphatic polyester obtained from α-hydroxy acids in different ways: ring-opening polymerization, and direct polycondensation. The use of one path over the other depends on …
Published in Nano Trends – A Journal of Nano Technology & Its Applications · Vol. 24, Issue 2, 2022 · pp. 38–46 Read article
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Method development and validation of RP-HPLC for simultaneous estimation of etoricoxib and Thiocolchicosidein tablet dosage forms
Abstract: Objective: A New approach became set up for simultaneous estimation of Etoricoxib and Thiocolchicoside with the aid of RP-HPLC approach.Methods: Chromatographic separations were carried using Agilent XDB C18, 150 x 4.6 mm, 5m, column with a mobile phase composition of Sodium Phospahte buffer 3.5 pH and Acetonitrile (20:80) Were delivered at a glide price of 1ml/min and the detection changed into done the usage of waters HPLC automobile sampler, separation …
Published in Journal of Modern Chemistry & Chemical Technology · Vol. 12, Issue 3, 2021 Read article
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In vitro Antibacterial Activity of Dietary Spice Trigonella foenum-graecum and Syzygium aromaticum
Abstract: Multi-drug resistant strains of bacteria are broadly distributed in hospitals and are progressively being isolated from community. The aim of the contemporary study was to govern the potential of spices Trigonella foenum-graecum (Fenugreek) and Syzygium aromaticum (Clove) as antibacterial mediators against drug resistant and virulent strains of gram negative pathogens quarantined from urinary pathway infections. Twelve bacterial isolates from urinary tract infections were studied for their ability to produce virulence …
Published in Research & Reviews : Journal of Herbal Science · Vol. 5, Issue 1, 2016 · pp. 6–11 Read article
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Grape Seed Extract: Potent Chemoprotective Agent: Phytochemical Analysis and GC-MS Study
Abstract: Plant extracts or secondary metabolites have served as antioxidants in phytotherapeutic medicines to protect against various diseases for centuries. Phytochemicals are naturally occurring in the medicinal plants, leaves, vegetables and roots that have defence mechanism and protect from various diseases. Phytochemicals are primary and secondary compounds. Chlorophyll, proteins and common sugars are included in primary constituents and secondary compounds include terpenoids, alkaloids, quinines, flavonoids, sterols, saponins, tannins and phenolic compounds. …
Published in Research & Reviews : Journal of Food Science & Technology · Vol. 5, Issue 3, 2016 · pp. 30–39 Read article
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In vitro Testing of the Antibacterial Activity of Root Extracts of Chlorophytum borivilianum against Organisms Causing Postnatal Infections
Abstract: ABSTRACTThe present study was carried out to study the antibacterial activities of root extracts of Chlorophytum borivilianum. The extract was prepared using different solvents like water, Tris-HCl, phosphate buffer, isopropanol, butanol-water and acetone. The antibacterial activity was tested against different bacteria like Staphylococcus aureus, Klebsiella, Proteus, Pseudomonas aeruginosa and Escherichia coli which cause postnatal infections, by agar diffusion method. Zone of inhibition produced by different extracts was tabulated. Butanol-water extract …
Published in Research & Reviews : Journal of Herbal Science · Vol. 1, Issue 3, 2012 · pp. 1–7 Read article
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Comparative Exploration of Medicinal and Photosynthetic Occurrences of Coriandrum sativum, Menthapiperita and Spinacia oleracea
Abstract: AbstractPlants form a large group of valuable materials like medicines, alkaloids, flavonoids, carotenes, and chlorophylls. To evaluate these activities leaves of three plant samples i.e., Coriandrum sativum, Mentha piperita and Spinacia oleracea were used in this study and two organic solvents i.e., acetone and ethanol were used to extract the antibacterials and antioxidants from plant samples. Total flavonoid content, total phenolic content and 1,1-Diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging methods were …
Published in Research & Reviews : Journal of Herbal Science · Vol. 2, Issue 3, 2013 · pp. 15–23 Read article
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High Performance Liquid Chromatographic Analysis and Antibacterial Activity of Methanolic Seed Extract of Syzygium cumini L.
Abstract: Syzygium cumini is a medicinally important plant widely used in traditional systems of medicines specifically Ayurveda followed in India. The seeds of the plant are recognized in various bio medicines for the treatment of diabetes. It is reasonable source of natural antioxidants used in dietary supplement of food and bioactive substances in cosmetics and pharmaceutical industries. In the present study, Syzygium cumini (jamun) seeds were collected from the Alagar Kovil …
Published in Research & Reviews : Journal of Herbal Science · Vol. 11, Issue 2, 2022 · pp. 23–30 Read article
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Antibacterial Activity and Preliminary Phytochemical Screening of the Extracts of the Bark of Moringa oleifera
Abstract: Ethyl acetate, acetone, methanol and aqueous extracts of the bark Moringa oleifera were screened against the enteric pathogens isolates namely, Esherichia coli, Shigella sonnei and Salmonella enteritis. All the extracts other than aqueous extract showed antimicrobial activity with the methanol extract having the percentage of activity. Preliminary phytochemical screening revealed the presence of and tannins in the methanol extract. Keywords: Moringa oleifera, bark, antimicrobial activity, flavones, tannins
Published in Research & Reviews: A Journal of Drug Design & Discovery · Vol. 1, Issue 2, 2014 · pp. 1–4 Read article
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Wound Healing Potential of Medicinal Plants: A Review
Abstract: Wound healing is an integrated cellular and biochemical process of restoring normal structure functions of damaged tissue. Healing is a natural phenomenon by which body itself overcome the damaged to the tissue, but the rate of healing is very slow and chance of microbial infection is high. Improvement in healing process can be accomplished either by shortening the time required for healing or to minimize the undesired consequences. India has …
Published in Research & Reviews: A Journal of Pharmacognosy · Vol. 4, Issue 2, 2017 · pp. 17–40 Read article
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Determination of RP-HPLC Method for Safinamide: Its Bulk and Tablet Dosage Form
Abstract: A new method was established for estimation of Safinamide by RP-HPLC method. The chromatographic conditions were successfully developed for the separation of Safinamide by using Agilent C18 column (4.6×150 mm, 5 µ); flow rate was 1 ml/min, mobile phase ratio was Methnol, Acetonitril (60:40), pH 3 (pH was adjusted with orthophosphoric acid), detection wavelength was 235 nm. The instrument used was WATERS HPLC Auto Sampler, Separation module 2695, photo diode …
Published in Research & Reviews: A Journal of Drug Formulation, Development and Production · Vol. 5, Issue 3, 2018 · pp. 1–6 Read article