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247 articles for “assay”
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Assay of the Antitrypanosomal Activity of Ziingiber Officinale Extract in Wister Rats
Abstract: This research investigated the antitrypanosomal effects of Zingiber officinale in rats infected with Trypanosoma brucei brucei. Twelve adult rats, 7 weeks old and of both sexes, were randomly divided into six groups (1 through 6), with each group consisting of two rats. All six groups (1–6) were intraperitoneally injected with 0.1 ml of blood containing 10⁶ Trypanosomes/ml. Groups 1–4 received daily intraperitoneal doses of the plant extract at concentrations of …
Published in Research and Reviews : Journal of Veterinary Science and Technology · Vol. 14, Issue 1, 2025 · pp. 32–37 Read article
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Zebra Fish Embryo Assay: A Wonderful Tool for Ecotoxicological Risk Assessment with Special Reference to Heavy Metals - An Overview
Abstract: In recent years, environmental pollution has become a pressing concern, prompting extensive research in aquatic ecotoxicology. With environmental degradation getting worse day by day, ecotoxicology research has gained a lot of attention. This area of study examines how biological communities in aquatic environments are affected by environmental contaminants. To unravel the toxicological mechanisms of these exogenous compounds, scientists turn to biological models. Several aquatic species, including zebrafish, toads, and big …
Published in International Journal of Toxins and Toxics · Vol. 2, Issue 2, 2025 · pp. 56–70 Read article
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Reference of Circadian Rhythm in the Treatment of Psoriasis: A Novel Insight
Abstract: The objective of the present study was to establish the needs for different products in the treatment of psoriasis with reference to circadian rhythm. The events like proliferation of keratinocytes and elastase activity are influenced by circadian rhythm. The proliferation of the keratinocytes occurs more during early hours of the day however, the enzymatic activities occur post to that. Psorolin ointment and Psorolin oil were studied for the cell proliferation …
Published in Research & Reviews : A Journal of Unani, Siddha and Homeopathy · Vol. 2, Issue 2, 2015 · pp. 1–7 Read article
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Development and Validation of Liquid Chromatography- Tandem Mass Spectrometric Method for Assay of Zidovudine in Human Urine
Abstract: A sensitive and selective liquid chromatographic-tandem mass spectrometric (LC-MSMS)method was developed to determine zidovudine (ZDV) in human urine. After solidphaseextraction with SPE cartridge, the urine sample was analyzed on a C18 column(Symmetry 3.5 μm; 75×4.6 mm i.d) interfaced with a triple quadrupole tandem massspectrometer. Positive electrospray ionization was employed as the ionization source. Themobile phase consisted of water-acetonitrile (20:80, v/v).The method was linear over aconcentration range of 10–1000 ng mL-1. …
Published in Research and Reviews: A Journal of Pharmaceutical Science · Vol. 5, Issue 3, 2014 · pp. 17–23 Read article
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Comparative Evaluation of In Vitro Antioxidant Activity of Bark, Flowers and Fruit Extracts of Pongamia pinnata
Abstract: In Indian history, the use of herbal remedies for various diseases has proved its efficacy. Free radicals are the main cause of disease such as diabetes, hypertension, inflammation, etc. The various parts of plant Pongamia pinnata possess good antioxidant activities. The objective of the study was to determine free radical scavenging activity of various plant parts by in vitro free radical scavenging assay such as DPPH assay, ABTS radical decolorization …
Published in Research & Reviews: A Journal of Pharmacognosy · Vol. 2, Issue 2, 2015 · pp. 14–21 Read article
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Phytochemical Screening and Biological Activity of Pomegranate Extracts
Abstract: Pomegranates are renowned for their abundance of bioactive compounds that exhibit potent antioxidant, anti-inflammatory, and antimicrobial properties, making them a subject of growing interest in natural medicine. This study aimed to compare the biological activities of ethanolic extracts derived from different parts of the pomegranate plant—specifically, the bark, peel, and leaves. A comprehensive investigation was conducted to evaluate their phytochemical composition and assess their antimicrobial, antioxidant, and anticancer properties. The …
Published in Research & Reviews: A Journal of Pharmacognosy · Vol. 12, Issue 2, 2025 · pp. 64–79 Read article
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Microbiological Analysis of Antibiotic: Polymyxin-B Sulphate
Abstract: This paper describes the different testing parameters and qualitative analysis of pharmaceuticals products. Pharmaceutical Industries employs microbiological analysis to improve the viability of drugs or other products such as preservatives, antibiotics, etc. Majorly tests of pharmaceutical products namely Antibiotic Assay, Sterility Test, Growth Promoting Test, and the Antimicrobial Preservatives Efficacy Test are done to check the viability or efficiency of these drugs & other pharmaceutical products to ensure the quality …
Published in Research and Reviews: A Journal of Microbiology and Virology · Vol. 12, Issue 1, 2022 · pp. 1–6 Read article
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Stimulation of Plant Growth Promoting Rhizobacteria with ZnO Nanoparticles to Improve Growth and Development of Groundnut Plants (Arachis Hypogaea L.)
Abstract: Rhizobacteria that stimulate plant growth have been extensively utilized as biofertilizers to improve the nutrient content of the soil for various crop plants. In the present study, we have isolated plant growth promoting rhizobacteria (PGPR) for rhizospheric soil of groundnut crops from Gujarat agricultural fields in the Saurashtra region. Fifty-two isolates with particular colony characteristics were obtained from rhizospheric soil. All strain was tested for their plant growth promoting (PGP) …
Published in Journal of Polymer & Composites · Vol. 12, Issue 6, 2024 · pp. 253–269 Read article
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Evaluation of Diagnostic Sensitivity of Rapid Immuno-Chromatographic Card Test (ICT) Against IgM ELISA for Serological Diagnosis of Scrub Typhus: An Experience at a Tertiary Care Centre in South-Eastern Rajasthan
Abstract: Introduction: Scrub typhus (ST) is a common but under-reported rickettsial disease in rural/sub-urban population of our country often resulting in severe complications and high mortality. Evaluation of ‘point of care’ tests like Rapid Immuno-Chromatographic Card Test (ICT) for diagnosis of ST is important to ascertain its utility in diagnosis of the disease in remote and peripheral settings. Aims: The present study was designed to evaluate the diagnostic sensitivity of Rapid …
Published in Research and Reviews: A Journal of Microbiology and Virology · Vol. 9, Issue 1, 2019 · pp. 85–91 Read article
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Laboratory Diagnosis of COVID-19—Lessons Learnt So Far: A Review of Literature
Abstract: The world has been experiencing a viral pandemic called as Coronavirus Disease-2019 (COVID-19) from beginning of 2020. The cases have been initially recorded in Wuhan, China but over a 4-month period (till the time this review is written) spread throughout the world causing severe morbidity and mortality. As it is a new viral infection, newer diagnostic techniques are in the pipeline. As of now, the recommended tests are the molecular …
Published in Research and Reviews: A Journal of Microbiology and Virology · Vol. 10, Issue 2, 2020 · pp. 14–21 Read article
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Stability Indicating HPLC Method for Drugs Indicated for Helicobacter Pylori-associated Ulcers
Abstract: Normal 0 false false false EN-US X-NONE AR-SA MicrosoftInternetExplorer4 Triple therapy regimen that comprises a proton pump inhibitor likes Omeprazole, antibiotics like Doxycycline and Tinidazole can be used for treatment of peptic ulceration which is caused by H. pylori. A validated HPLC method was developed as a stability-indicating HPLC method for these drugs and its impurities. New method overcame interference between Doxycycline and its epimer on achiral column by using …
Published in Research and Reviews: A Journal of Pharmaceutical Science · Vol. 6, Issue 1, 2015 · pp. 28–46 Read article
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Detection of Listeria monocytogenes in Milk and Ice Cream by Polymerase Chain Reaction
Abstract: Abstract The present study was undertaken to standardize PCR assay for detection of L. monocytogenes and Listeriolysin O from livestock foods and compare its efficacy with conventional cultural methods. A set of primer derived from iap gene and other set derived from hlyA gene were used for detection of L. monocytogenes and Listeriolysin O in the PCR assay. The PCR assay was initially standardized by optimizing the concentration of the …
Published in Research and Reviews: A Journal of Microbiology and Virology · Vol. 5, Issue 1, 2015 · pp. 24–35 Read article
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A Brief Introduction on the Electrokinetic Mixing and Separation in Microfluidic Systems
Abstract: Electrokinetics encompasses the analysis of fluid or particle motion in the electrical field; includes electroosmosis, electrophoresis, dielectrophoresis, electro-wetting, etc. Electrokinetic applications in the production of microfluidic systems have become quite attractive over the last decade. Electro-kinetic systems usually do not need any additional mechanical moving parts and may be made compact by removing the power source with a small battery. Electro-kinetic based microfluidic systems may also serve as a feasible …
Published in Journal of Aerospace Engineering & Technology · Vol. 10, Issue 3, 2020 · pp. 24–34 Read article
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Identifying ABO Blood Group and Rh Status Using Dried Saliva Samples
Abstract: This article discusses the accuracy of dried saliva samples in establishing ABO and Rh blood types. Participants, likely screened for secretor status beforehand, submitted blood and saliva samples. Both samples were ABO blood typed (A, B, AB, or O) using standard methods such as gel card technology or hemagglutination assays. We used both saliva and blood to perform Rh typing. The findings revealed that saliva and blood typing for ABO …
Published in International Journal of Pathogens · Vol. 1, Issue 1, 2024 · pp. 11–19 Read article
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Evaluation of Antioxidant and Cytotoxic activities of Mycelia Biomass and Culture broth of three Edible Mushrooms, Morchella conica, Hericium erinaceus and Pleurotus florida Grown in Submerged Culture
Abstract: Mycelia biomass of three edible mushrooms namely, Morchella conica, Pleurotus florida and Hericium erinaceus were grown as submerged culture in potato dextrose broth supplemented with yeast extract, potassium dihydrogen orthophosphate and magnesium sulphate. The mycelia biomass was extracted with 70% ethanol and culture broth with ethyl acetate. The solvents were evaporated to dryness at low temperature using a rotary vacuum evaporator and the residues thus obtained were used for the …
Published in International Journal of Fungi · Vol. 1, Issue 1, 2024 · pp. 37–50 Read article
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Percentage Changes According to Clinical Pathological Findings of Acute Rotavirus Gastroenteritis in Children
Abstract: Acute Rotavirus gastroenteritis in children, called “False Cholera”, is one of the children’s diarrheal diseases. Among symptomatic children with acute Rotavirus gastroenteritis, the chief clinical manifestations include diarrhoea, vomiting, fever and dehydration. We did research with such aims as follows to differentiate Acute Rotavirus Gastroenteritis in Children, with significant morbidity, from others in paediatric gastroenterology, and to introduce its etiological treatment. We selected 87 subjects who died from children’s diarrheal …
Published in Research and Reviews : A Journal of Medical Science and Technology · Vol. 13, Issue 2, 2024 · pp. 51–57 Read article
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Development of a Polymeric Detection System for Salivary Annexin-1: A Potential Tool for Point-of-Care Diagnostics in Periodontal Disease
Abstract: Salivary annexin-1, a protein with anti-inflammatory properties, has emerged as a potential biomarker for periodontal disease. However, current detection methods, like ELISA, are often complex and require laboratory settings. This study explores the development of a novel polymeric detection system for the rapid and sensitive identification of salivary annexin-1. This system could leverage the unique properties of polymers to create a point-of-care diagnostic tool for: (1) early detection and monitoring …
Published in Journal of Polymer & Composites · Vol. 12, Issue 5, 2024 · pp. 101–110 Read article
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Lab Reagents and Their Importance in Bio-chemistry
Abstract: Biochemical reagents are essential substances in the study of life at the molecular level. These reagents help scientists detect, identify, and quantify bio-molecules such as proteins, carbohydrates, nucleic acids, and enzymes in biological systems. They play a vital role in understanding metabolic reactions and physiological processes within living organisms. Biochemical reagents are generally classified into analytical, diagnostic, enzymatic, chromogenic, and buffer reagents, each serving a specific purpose in laboratory and …
Published in Research and Reviews : A Journal of Biotechnology · Vol. 16, Issue 1, 2026 · pp. 1–4 Read article
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CRISPR, Recombinant DNA, and LAMP-Based Platforms for Monkeypox: Emerging Tools for Diagnosis and Therapeutic Development
Abstract: Monkeypox, a zoonotic viral infection caused by the Monkeypox virus of the Orthopoxvirus genus, has recurred as a worldwide public health concern after recent outbreaks outside of its classical endemic areas in Central and West Africa. The resurgence has demanded a thorough reassessment of its epidemiology, mode of transmission, and clinical presentation. In light of these challenges, recombinant DNA technology (rDNA) has emerged as a candidate for developing vaccines, diagnostics, …
Published in International Journal of Virus Studies · Vol. 3, Issue 1, 2026 · pp. 7–28 Read article
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Pathogenesis, Treatment and Molecular Mechanisms for Development of Multidrug-resistant (MDR) TB as well as Identification of MDR Mycobacterium Tuberculosis
Abstract: Normal 0 false false false EN-US X-NONE X-NONE Tuberculosis (TB) is a medical, social, and economic disaster of huge magnitude in the world. Prevalence of TB has increased due to poverty, population growth, migration and a high number of TB cases in HIV positive individuals. High prevalence rate of M. tuberculosis strains with multidrug resistance (MDR) is a serious problem as global emergency. Strains of M. tuberculosis resistant to both …
Published in Research and Reviews: A Journal of Microbiology and Virology · Vol. 3, Issue 1, 2013 · pp. 16–38 Read article