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International Journal of Toxins and Toxics Original Research

Zebra Fish Embryo Assay: A Wonderful Tool for Ecotoxicological Risk Assessment with Special Reference to Heavy Metals - An Overview

  1. Navendu Sharma Department of Toxicology,Chaudhary Charan Singh University
  2. Sakshi Goswami Department of Toxicology,Chaudhary Charan Singh University
  3. Meenu Singh Department of Toxicology,Chaudhary Charan Singh University
  4. Varsha.Rani Department of Toxicology,Chaudhary Charan Singh University
  5. Yeshvandra Verma Department of Toxicology,Chaudhary Charan Singh University
  6. S.V.S.Rana Department of Toxicology,Chaudhary Charan Singh University

Abstract

In recent years, environmental pollution has become a pressing concern, prompting extensive research in aquatic ecotoxicology. With environmental degradation getting worse day by day, ecotoxicology research has gained a lot of attention. This area of study examines how biological communities in aquatic environments are affected by environmental contaminants. To unravel the toxicological mechanisms of these exogenous compounds, scientists turn to biological models. Several aquatic species, including zebrafish, toads, and big fleas, have been used as model organisms and developed for ecotoxicity assessment. The ability to integrate genetic, cellular, and whole organism endpoints, their tiny size, simple replication, minimal care costs, and short life cycle have made them increasingly desirable tools for researching the toxicity and bioaccumulation of environmental contaminants. Researchers selecting model animals for aquatic ecotoxicology studies might use this review as a guide. Zebrafish have been utilised as models in the field of aquatic ecotoxicology study. Its toxicity to environmental toxins is evaluated using phenotypic analysis, genetics, biomarkers, and developmental toxicity.

Keywords

References (72)

  1. Anderson SL, Wild GC. Linking genotoxic responses and reproductive success in ecotoxicology. Environmental Health Perspectives. 1994;102(suppl12):9-12. doi:10.1289/ehp.94102s129
  2. Ayotte JD, Montgomery DL, Flanagan SM, Robinson KW. Arsenic in Groundwater in Eastern New England: Occurrence, Controls, and Human Health Implications. Environmental Science & Technology. 2003;37(10):2075-2083. doi:10.1021/es026211g
  3. Bakulski KM, Seo YA, Hickman RC, Brandt D, Vadari HS, Hu H, et al. Heavy Metals Exposure and Alzheimer’s Disease and Related Dementias. Journal of Alzheimer's Disease. 2020;76(4):1215-1242. doi:10.3233/jad-200282
  4. Balciunas D, Davidson AE, Sivasubbu S, Hermanson SB, Welle Z, Ekker SC. Enhancer trapping in zebrafish using the Sleeping Beauty transposon. BMC Genomics. 2004;5(1). doi:10.1186/1471-2164-5-62
  5. Bavanilathamuthiah, Lakshmanan Y, Purnima, Ratnaprabha. Developmental Toxicity of Arsenic and its Underlying Mechanisms in the early Embryonic Development. Research Journal of Pharmacy and Technology. 2016;9(4):340. doi:10.5958/0974-360x.2016.00060.3
  6. Blechinger SR, Warren JT, Kuwada JY, Krone PH. Developmental toxicology of cadmium in living embryos of a stable transgenic zebrafish line. Environmental Health Perspectives. 2002;110(10):1041-1046. doi:10.1289/ehp.021101041
  7. Brown RE. An introduction to neuroendocrinology. Cambridge University Press; 1994 Jan 27.
  8. Brown, ME. 2013. The Physiology of Fishes. Academic Press. http://books.google.ie/books?id=hxvgBAAAQBAJ&printsec=frontcover&dq=The+physiology+of+fishes&hl=&cd=9&source=gbs_api.
  9. Bumcrot DA, McMahon AP. Somite Differentiation: Sonic signals somites. Current Biology. 1995;5(6):612-614. doi:10.1016/s0960-9822(95)00123-0
  10. Capriello T, Grimaldi MC, Cofone R, D'Aniello S, Ferrandino I. Effects of aluminium and cadmium on hatching and swimming ability in developing zebrafish. Chemosphere. 2019;222:243-249. doi:10.1016/j.chemosphere.2019.01.140
  11. Cariccio VL, Samà A, Bramanti P, Mazzon E. Mercury Involvement in Neuronal Damage and in Neurodegenerative Diseases. Biological Trace Element Research. 2018;187(2):341-356. doi:10.1007/s12011-018-1380-4
  12. Carlson P, Smalley DM, Van Beneden RJ. Proteomic Analysis of Arsenic-Exposed Zebrafish (Danio rerio) Identifies Altered Expression in Proteins Involved in Fibrosis and Lipid Uptake in a Gender-Specific Manner. Toxicological Sciences. 2013;134(1):83-91. doi:10.1093/toxsci/kft086
  13. Carmany-Rampey A, Moens CB. Modern mosaic analysis in the zebrafish. Methods. 2006;39(3):228-238. doi:10.1016/j.ymeth.2006.02.002
  14. Ceccatelli S, Daré E, Moors M. Methylmercury-induced neurotoxicity and apoptosis. Chemico-Biological Interactions. 2010;188(2):301-308. doi:10.1016/j.cbi.2010.04.007
  15. Chaurasia MK, Ravichandran G, Nizam F, Arasu MV, Al-Dhabi NA, Arshad A, Harikrishnan R, Arockiaraj J. 2016. In-silico analysis and mRNA modulation of detoxification enzymes GST delta and kappa against various biotic and abiotic oxidative stressors. Fish & shellfish immunology. 54:353-63. https://doi.org/10.1016/j.fsi.2015.11.034.
  16. Cheng SH, Wai AWK, So CH, Wu RSS. Cellular and molecular basis of cadmium-induced deformities in zebrafish embryos. Environmental Toxicology and Chemistry. 2000;19(12):3024-3031. doi:10.1002/etc.5620191223
  17. Covassin L, Amigo JD, Suzuki K, Teplyuk V, Straubhaar J, Lawson ND. Global analysis of hematopoietic and vascular endothelial gene expression by tissue specific microarray profiling in zebrafish. Developmental Biology. 2006;299(2):551-562. doi:10.1016/j.ydbio.2006.08.020
  18. Crump KL, Trudeau VL. Mercury-induced reproductive impairment in fish. Environmental Toxicology and Chemistry. 2009;28(5):895-907. doi:10.1897/08-151.1
  19. Dai YJ, Jia YF, Chen N, Bian WP, Li QK, Ma YB, et al. Zebrafish as a model system to study toxicology. Environmental Toxicology and Chemistry. 2014;33(1):11-17. doi:10.1002/etc.2406
  20. De Boeck M, Kirsch-Volders M, Lison D. Cobalt and antimony: genotoxicity and carcinogenicity. Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis. 2003;533(1-2):135-152. doi:10.1016/j.mrfmmm.2003.07.012
  21. Alvarez MDC, Murphy CA, Rose KA, McCarthy ID, Fuiman LA. Maternal body burdens of methylmercury impair survival skills of offspring in Atlantic croaker (Micropogonias undulatus). Aquatic Toxicology. 2006;80(4):329-337. doi:10.1016/j.aquatox.2006.09.010
  22. Deng, P., Ma, Q., Xi, Y., Yang, L., Lin, M., Yu, Z., ...& Zhou, Z. (2020). Transcriptomic insight into cadmium-induced neurotoxicity in embryonic neural stem/progenitor cells. Toxicology in Vitro, 62, 104686.
  23. Dietrich MO, Mantese CE, Anjos GD, Souza DO, Farina M. Motor impairment induced by oral exposure to methylmercury in adult mice. Environmental Toxicology and Pharmacology. 2005;19(1):169-175. doi:10.1016/j.etap.2004.07.004
  24. Farina M, Aschner M, Rocha JBT. Oxidative stress in MeHg-induced neurotoxicity. Toxicology and Applied Pharmacology. 2011;256(3):405-417. doi:10.1016/j.taap.2011.05.001
  25. Figgitt M, Newson R, Leslie IJ, Fisher J, Ingham E, Case CP. The genotoxicity of physiological concentrations of chromium (Cr(III) and Cr(VI)) and cobalt (Co(II)): An in vitro study. Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis. 2010;688(1-2):53-61. doi:10.1016/j.mrfmmm.2010.03.008
  26. Finley KR, Davidson AE, Ekker SC. Three-Color Imaging Using Fluorescent Proteins in Living Zebrafish Embryos. BioTechniques. 2001;31(1):66-72. doi:10.2144/01311st02
  27. Fishman MC. Zebrafish--the Canonical Vertebrate. Science. 2001;294(5545):1290-1291. doi:10.1126/science.1066652
  28. Ghatak S, Biswas A, Dhali GK, Chowdhury A, Boyer JL, Santra A. Oxidative stress and hepatic stellate cell activation are key events in arsenic induced liver fibrosis in mice. Toxicology and Applied Pharmacology. 2011;251(1):59-69. doi:10.1016/j.taap.2010.11.016
  29. Gibb H, O’Leary KG. Mercury Exposure and Health Impacts among Individuals in the Artisanal and Small-Scale Gold Mining Community: A Comprehensive Review. Environmental Health Perspectives. 2014;122(7):667-672. doi:10.1289/ehp.1307864
  30. Hall C, Flores MV, Storm T, Crosier K, Crosier P. The zebrafish lysozyme C promoter drives myeloid-specific expression in transgenic fish. BMC Developmental Biology. 2007;7(1). doi:10.1186/1471-213x-7-42
  31. Halloran MC, Sato-Maeda M, Warren JT, Su F, Lele Z, Krone PH, et al. Laser-induced gene expression in specific cells of transgenic zebrafish. Development. 2000;127(9):1953-1960. doi:10.1242/dev.127.9.1953
  32. Hardy ME, Ross LV, Chien CB. Focal gene misexpression in zebrafish embryos induced by local heat shock using a modified soldering iron. Developmental Dynamics. 2007;236(11):3071-3076. doi:10.1002/dvdy.21318
  33. Hay BN. Deletion 22q11: Spectrum of Associated Disorders. Seminars in Pediatric Neurology. 2007;14(3):136-139. doi:10.1016/j.spen.2007.07.005
  34. Hen Chow ES. Cadmium Affects Muscle Type Development and Axon Growth in Zebrafish Embryonic Somitogenesis. Toxicological Sciences. 2003;73(1):149-159. doi:10.1093/toxsci/kfg046
  35. Ho RK, Kane DA. Cell-autonomous action of zebrafish spt-1 mutation in specific mesodermal precursors. Nature. 1990;348(6303):728-730. doi:10.1038/348728a0
  36. Holder N, Xu Q. Microinjection of DNA, RNA, and Protein into the Fertilized Zebrafish Egg for Analysis of Gene Function. Molecular Embryology. 487-490. doi:10.1385/1-59259-270-8:487
  37. Howe K, Clark MD, Torroja CF, Torrance J, Berthelot C, Muffato M, et al. The zebrafish reference genome sequence and its relationship to the human genome. Nature. 2013;496(7446):498-503. doi:10.1038/nature12111
  38. Hughes MF, Beck BD, Chen Y, Lewis AS, Thomas DJ. Arsenic Exposure and Toxicology: A Historical Perspective. Toxicological Sciences. 2011;123(2):305-332. doi:10.1093/toxsci/kfr184
  39. Jessen JR, Meng A, McFarlane RJ, Paw BH, Zon LI, Smith GR, et al. Modification of bacterial artificial chromosomes through Chi-stimulated homologous recombination and its application in zebrafish transgenesis. Proceedings of the National Academy of Sciences. 1998;95(9):5121-5126. doi:10.1073/pnas.95.9.5121
  40. Jowett T, Yan YL. Double fluorescent in situ hybridization to zebrafish embryos. Trends in Genetics. 1996;12(10):387-389. doi:10.1016/s0168-9525(96)90091-8
  41. Karagas MR, Stukel TA, Tosteson TD. Assessment of cancer risk and environmental levels of arsenic in New Hampshire. International Journal of Hygiene and Environmental Health. 2002;205(1-2):85-94. doi:10.1078/1438-4639-00133
  42. Kim, H. J., Mahboob, S., Viayaraghavan, P., Al-Ghanim, K. A., Al-Misned, F., Kim, Y. O., & Ahmed, Z. (2020). Determination of toxic effects of lead acetate on different sizes of zebra fish (Danio rerio) in soft and hard water. Journal of King Saud University-Science, 32(2), 1390-1394.
  43. Kimmel CB, Ballard WW, Kimmel SR, Ullmann B, Schilling TF. Stages of embryonic development of the zebrafish. Developmental Dynamics. 1995;203(3):253-310. doi:10.1002/aja.1002030302
  44. Komoike Y, Matsuoka M. Developmental adverse effects of trace amounts of lead: Evaluation using zebrafish model. Frontiers in Pharmacology. 2022;13. doi:10.3389/fphar.2022.1014912
  45. Kumaresan V, Bhatt P, Ganesh MR, Harikrishnan R, Arasu M, Al-Dhabi NA, et al. A novel antimicrobial peptide derived from fish goose type lysozyme disrupts the membrane of Salmonella enterica. Molecular Immunology. 2015;68(2):421-433. doi:10.1016/j.molimm.2015.10.001
  46. Kumaresan V, Ravichandran G, Nizam F, Dhayanithi NB, Arasu MV, Al-Dhabi NA, et al. Multifunctional murrel caspase 1, 2, 3, 8 and 9: Conservation, uniqueness and their pathogen-induced expression pattern. Fish & Shellfish Immunology. 2016;49:493-504. doi:10.1016/j.fsi.2016.01.008
  47. Kurosawa, K., Egashira, K., Tani, M., Jahiruddin, M., Moslehuddin, A. Z. M., & Rahman, Z. M. (2008). Groundwater–soil–crop relationship with respect to arsenic contamination in farming villages of Bangladesh–a preliminary study. Environmental Pollution, 156(2), 563-565.
  48. Lauwerys R, Lison D. Health risks associated with cobalt exposure — an overview. Science of The Total Environment. 1994;150(1-3):1-6. doi:10.1016/0048-9697(94)90125-2
  49. Lee D, Park C, Lee H, Lugus JJ, Kim SH, Arentson E, et al. ER71 Acts Downstream of BMP, Notch, and Wnt Signaling in Blood and Vessel Progenitor Specification. Cell Stem Cell. 2008;2(5):497-507. doi:10.1016/j.stem.2008.03.008
  50. Lee J, Freeman JL. Embryonic exposure to 10 μg L−1lead results in female-specific expression changes in genes associated with nervous system development and function and Alzheimer's disease in aged adult zebrafish brain. Metallomics. 2016;8(6):589-596. doi:10.1039/c5mt00267b
  51. Long Q, Meng A, Wang H, Jessen JR, Farrell MJ, Lin S. GATA-1 expression pattern can be recapitulated in living transgenic zebrafish using GFP reporter gene. Development. 1997;124(20):4105-4111. doi:10.1242/dev.124.20.4105
  52. Monson BA. Trend Reversal of Mercury Concentrations in Piscivorous Fish from Minnesota Lakes: 1982−2006. Environmental Science & Technology. 2009;43(6):1750-1755. doi:10.1021/es8027378
  53. Parinov S, Kondrichin I, Korzh V, Emelyanov A. Tol2 transposon‐mediated enhancer trap to identify developmentally regulated zebrafish genes in vivo. Developmental Dynamics. 2004;231(2):449-459. doi:10.1002/dvdy.20157
  54. Parng C, Seng WL, Semino C, McGrath P. Zebrafish: A Preclinical Model for Drug Screening. ASSAY and Drug Development Technologies. 2002;1(1):41-48. doi:10.1089/154065802761001293
  55. Peterson RT, Link BA, Dowling JE, Schreiber SL. Small molecule developmental screens reveal the logic and timing of vertebrate development. Proceedings of the National Academy of Sciences. 2000;97(24):12965-12969. doi:10.1073/pnas.97.24.12965
  56. Piotrowski T, Ahn DG, Schilling TF, Nair S, Ruvinsky I, Geisler R, et al. The zebrafish van gogh mutation disrupts tbx1 , which is involved in the DiGeorge deletion syndrome in humans. Development. 2003;130(20):5043-5052. doi:10.1242/dev.00704
  57. Piotrowski T, Schilling TF, Brand M, Jiang YJ, Heisenberg CP, Beuchle D, et al. Jaw and branchial arch mutants in zebrafish II: anterior arches and cartilage differentiation. Development. 1996;123(1):345-356. doi:10.1242/dev.123.1.345
  58. Rasinger J, Lundebye AK, Penglase S, Ellingsen S, Amlund H. Methylmercury Induced Neurotoxicity and the Influence of Selenium in the Brains of Adult Zebrafish (Danio rerio). International Journal of Molecular Sciences. 2017;18(4):725. doi:10.3390/ijms18040725
  59. Reinardy HC, Syrett JR, Jeffree RA, Henry TB, Jha AN. Cobalt-induced genotoxicity in male zebrafish (Danio rerio), with implications for reproduction and expression of DNA repair genes. Aquatic Toxicology. 2013;126:224-230. doi:10.1016/j.aquatox.2012.11.007
  60. Risch MR, Gay DA, Fowler KK, Keeler GJ, Backus SM, Blanchard P, et al. Spatial patterns and temporal trends in mercury concentrations, precipitation depths, and mercury wet deposition in the North American Great Lakes region, 2002–2008. Environmental Pollution. 2012;161:261-271. doi:10.1016/j.envpol.2011.05.030
  61. Sankhla MS, Sharma K, Kumar R. 2017.Heavy metal causing neurotoxicity in human health. International Journal of Innovative Research in Science. Engineering and Technology.6(5). doi:10.15680/IJIRSET.2017.0605054.
  62. Scheuhammer AM, Meyer MW, Sandheinrich MB, Murray MW. 2007.Effects of environmental methylmercury on the health of wild birds, mammals, and fish. AMBIO: a Journal of the Human Environment. 36(1):12-9. https://doi.org/10.1579/0044-7447(2007)36[12:EOEMOT]2.0.CO;2.
  63. Singh M, Khan H, Verma Y, Rana SVS. Distinctive fingerprints of genotoxicity induced by As, Cr, Cd, and Ni in a freshwater fish. Environmental Science and Pollution Research. 2019;26(19):19445-19452. doi:10.1007/s11356-019-05274-z
  64. Stabler SP, Allen RH. VITAMIN B12 DEFICIENCY AS A WORLDWIDE PROBLEM. Annual Review of Nutrition. 2004;24(1):299-326. doi:10.1146/annurev.nutr.24.012003.132440
  65. Sumanas S, Gomez G, Zhao Y, Park C, Choi K, Lin S. Interplay among Etsrp/ER71, Scl, and Alk8 signaling controls endothelial and myeloid cell formation. Blood. 2008;111(9):4500-4510. doi:10.1182/blood-2007-09-110569
  66. Sumanas S, Lin S. Ets1-Related Protein Is a Key Regulator of Vasculogenesis in Zebrafish. PLoS Biology. 2005;4(1):e10. doi:10.1371/journal.pbio.0040010
  67. Thisse C, Thisse B. High-resolution in situ hybridization to whole-mount zebrafish embryos. Nature Protocols. 2007;3(1):59-69. doi:10.1038/nprot.2007.514
  68. Triantafyllidou S, Lambrinidou Y, Edwards M. 2009. Lead (Pb) exposure through drinking water: Lessons to be learned from recent US experience. Global NEST Journal. 11(3):341.
  69. The Physiology of Fishes. 2005. doi:10.1201/9781420058093
  70. Verma Y, Rani V, Singh Rana SV. Assessment of cadmium sulphide nanoparticles toxicity in the gills of a fresh water fish. Environmental Nanotechnology, Monitoring & Management. 2020;13:100280. doi:10.1016/j.enmm.2019.100280
  71. Yesildag K, Gur C, Ileriturk M, Kandemir FM. Evaluation of oxidative stress, inflammation, apoptosis, oxidative DNA damage and metalloproteinases in the lungs of rats treated with cadmium and carvacrol. Molecular Biology Reports. 2021;49(2):1201-1211. doi:10.1007/s11033-021-06948-z
  72. Zhang J, Peterson SM, Weber GJ, Zhu X, Zheng W, Freeman JL. Decreased axonal density and altered expression profiles of axonal guidance genes underlying lead (Pb) neurodevelopmental toxicity at early embryonic stages in the zebrafish. Neurotoxicology and Teratology. 2011;33(6):715-720. doi:10.1016/j.ntt.2011.07.010