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8 articles for “serial dilution”
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Isolation of Commercially Important Microalgae Species from the South-Eastern Gulf of Lake Tana, Ethiopia
Abstract: Microalgae are simple photosynthetic microorganisms that can be cultivated in controlled environmental conditions for different economic and environmental applications. The presentstudy was aimed to isolate commercially important local microalgae species in a laboratory that is suitable for mass production. The sample was taken from the South-Eastern Gulf of Lake Tana from February 2020to January 2021. The isolation was carried out using agar plating technique, serial dilution and capillary pipette methods …
Published in Research and Reviews : A Journal of Biotechnology · Vol. 12, Issue 3, 2022 · pp. 30–37 Read article
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Determining the Antagonistic Potential of Lactobacilli Isolated from Commercial Curd Samples against the E. coli
Abstract: Curd has always been an important source exhibiting a plethora of Lactobacilli. Lactobacilli are rod-shaped bacteria, capable of producing short-chain fatty acids, lactate, and acetate by carbohydrate fermentation process. A study was done to determine the antimicrobial activity of isolated and identified Lactobacilli bacteria from commercial curd samples against standard strains of E. coli. All the 10 samples were found have lactobacilli strains. The antagonistic activity of these strains was …
Published in Research & Reviews : Journal of Food Science & Technology · Vol. 9, Issue 2, 2020 · pp. 27–30 Read article
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Anti-cancerous Activity of the Rhizome Extract of Alpinia galanga L. Willd. on the Human Liver Carcinoma Cells
Abstract: The rhizome of Alpinia galanga. L. (Willd). was collected from three places i.e, Udupi Shankara narayana temple surrounding area, Shringeri and Madikeri accessions. About 100 gms of the rhizome was taken, dried and powdered to obtain 1 gm of the extract through methanol as the solvent. 1 gm of the solid extract was subjected for filteration through millipore syringe filter., to this about 6ml of cell based assay buffer and …
Published in Research and Reviews : A Journal of Biotechnology · Vol. 8, Issue 2, 2018 · pp. 1–6 Read article
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Isolation, Production and Purification of Antibiotics Isolation from Microbes from Soil
Abstract: A chemical compound acting against life is called antibiotics. The term “antibiotic” literally means “against life”. The term appeared as early as 1928 in the French microbiological literature as antibiosis. The phenomenon of antagonism between living organisms was frequently observed even since 1877, when Pasteur and Joubert noticed that aerobic bacteria antagonized the growth of Bacillus anthracis. However, the world in its present restrictive meaning, “a chemical substance derived from …
Published in Research and Reviews : A Journal of Life Sciences · Vol. 4, Issue 1, 2014 · pp. 10–18 Read article
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Screening and Isolation of Bacterial Isolates for Polyhydroxyalkanoates Production from Oil-Contaminated sites
Abstract: Synthetic polymers are generally manufactured from petroleum and chemical-based sources. These polymers are employed in day-to-day life basic needs which leads to the production of huge number of synthetic polymers that accumulate in nature for thousands of years. There are limitations associated with synthetic polymers apart from their non-biodegradability, non-compatibility their mechanical and physical properties that cannot be easily altered narrows their few applications. PHAs (polyhydroxyalkanoates) are biopolymers that can …
Published in Research and Reviews: A Journal of Microbiology and Virology · Vol. 14, Issue 1, 2024 · pp. 1–6 Read article
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Impact of Age Difference and Diet of Mother on the Microbiota of Human Breast Milk
Abstract: Aim: This study aimed to investigate the impact of age difference and diet of mother on the microbiota of Human Breast Milk. Human Breast milk is a complex bio-fluid that provides the nutrients and bioactive agents, including bacteria, which are necessary for the development of the infant gut microbiota. Human milk microorganisms contribute significantly for the healthy development of the immune system in infants, along with shaping the gut microbiota. …
Published in Research and Reviews: A Journal of Health Professions · Vol. 12, Issue 1, 2022 · pp. 32–38 Read article
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Differential Susceptibility of Bacteriophage and Viruses to Reactive Oxygen Species
Abstract: Normal 0 false false false EN-US X-NONE X-NONE Reactive oxygen species of superoxide, hydrogen peroxide, and hydroxyl radicals were generated by mixing xanthine, xanthine oxidase and iron citrate and incubating for 5 min at room temperature. T2 bacteriophage, encephalomyocarditis (EMC) virus, adenovirus, or vaccinia virus particles were incubated with the solution for various periods of time after reactive oxygen species were formed. Physical damage to the virus particles that affected …
Published in Research and Reviews: A Journal of Microbiology and Virology · Vol. 6, Issue 2, 2016 · pp. 7–12 Read article
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Detection of Listeria monocytogenes in Milk and Ice Cream by Polymerase Chain Reaction
Abstract: Abstract The present study was undertaken to standardize PCR assay for detection of L. monocytogenes and Listeriolysin O from livestock foods and compare its efficacy with conventional cultural methods. A set of primer derived from iap gene and other set derived from hlyA gene were used for detection of L. monocytogenes and Listeriolysin O in the PCR assay. The PCR assay was initially standardized by optimizing the concentration of the …
Published in Research and Reviews: A Journal of Microbiology and Virology · Vol. 5, Issue 1, 2015 · pp. 24–35 Read article