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Research and Reviews: A Journal of Microbiology and Virology Original Research

Screening and Isolation of Bacterial Isolates for Polyhydroxyalkanoates Production from Oil-Contaminated sites

  1. Rakhi Pandey Department of Biotechnology, Jaypee institute of Information Technology, Noida
  2. Garima Mathur Department of Biotechnology, Jaypee institute of Information Technology, Noida

Abstract

Synthetic polymers are generally manufactured from petroleum and chemical-based sources. These polymers are employed in day-to-day life basic needs which leads to the production of huge number of synthetic polymers that accumulate in nature for thousands of years. There are limitations associated with synthetic polymers apart from their non-biodegradability, non-compatibility their mechanical and physical properties that cannot be easily altered narrows their few applications. PHAs (polyhydroxyalkanoates) are biopolymers that can be a promising solution to this problem, and it is one of the major candidates in reducing the dependency on synthetic polymers. In this study, oil-contaminated soil samples were collected from 2 cm depth from different locations. Soil samples were serially diluted and were used for the isolation of bacterial colonies on nutrient agar enriched with 2% glucose. Seven isolates were obtained after incubation and were screened for PHA production potential using Sudan black B and Nile blue sulfate. Results showed that out of seven isolated colonies, only three were found positive for primary Sudan black B screening, and out of 3 only 2 were found positive for confirmatory Nile blue screening. There are only two isolates that were positive for both screening dyes and were selected for further studies on PHA production. Furthermore, morphological characterization and biochemical characterization of these two isolates are done for the identification of bacterial isolates.

Keywords

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