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49 articles for “and HPLC analysis.”
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A Systematic Review of Method Development and Validation for Ramipril Analysis Using Hplc in Cardiovascular Research
Abstract: The field of analytical chemistry encompasses a variety of methods that are crucial in determining the chemical composition of different substances. UV-visible spectroscopy and high-performance liquid chromatography (HPLC) are two widely utilized methods that are the subject of this article. UV-visible spectroscopy uses the Beer-Lambert law to quantify light absorption, which can provide information about the concentration of absorbing species. The method is based on how light and matter interact, …
Published in Journal of Modern Chemistry & Chemical Technology · Vol. 15, Issue 2, 2024 · pp. 37–44 Read article
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Optimizing Antibiotic Analysis: A Comprehensive Guide to HPLC Process of Assessment and Quantification
Abstract: The history and uses of chromatography are thoroughly covered in this work, with an emphasis on the use of High-Pressure Liquid Chromatography (HPLC) in pharmaceutical analysis. Since its discovery by Russian botanist Mikhail Tswett in the 19th century, chromatography has undergone tremendous developments that have given rise to a variety of chromatography types and applications. The story follows the evolution historically, emphasizing Tswett's groundbreaking breakthrough in plant color classification and …
Published in International Journal of Antibiotics · Vol. 1, Issue 2, 2024 · pp. 27–44 Read article
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High Performance Liquid Chromatographic Analysis and Antibacterial Activity of Methanolic Seed Extract of Syzygium cumini L.
Abstract: Syzygium cumini is a medicinally important plant widely used in traditional systems of medicines specifically Ayurveda followed in India. The seeds of the plant are recognized in various bio medicines for the treatment of diabetes. It is reasonable source of natural antioxidants used in dietary supplement of food and bioactive substances in cosmetics and pharmaceutical industries. In the present study, Syzygium cumini (jamun) seeds were collected from the Alagar Kovil …
Published in Research & Reviews : Journal of Herbal Science · Vol. 11, Issue 2, 2022 · pp. 23–30 Read article
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Chemical Composition Quantification of Components in Extracts of Uncaria tomentosa by HPLC and LC-MS Analysis
Abstract: The tropical medicinal vine known as cat's claw, Uncaria tomentosa (Willd. ex Schult.) DC. (Family: Rubiaceae), is native to the Amazon rainforest and other regions of South and Central America. It has been shown to be useful as an immune system booster, antioxidant, antibacterial, and anti-inflammatory agent and has been historically used to treat wounds, fever, urinary tract infections, abscesses, and asthma. U. tomentosa contains a variety of phytoconstituents, including …
Published in Journal of Catalyst & Catalysis · Vol. 10, Issue 1, 2023 · pp. 32–39 Read article
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Advancements in Method Development and Validation for Levocetirizine: A Comprehensive Review
Abstract: This review article delves into the intricate realm of concurrent estimation of multiple compounds, with a specific emphasis on Levocetirizine and its related substances. It looks into how to improve analytical process specificity by using reverse phase high-performance liquid chromatography (RP-HPLC) in conjunction with UV detection. The study meticulously explores the intricacies of method development and validation, offering invaluable insights into the realms of precision and accuracy within pharmaceutical analysis. …
Published in Research & Reviews: A Journal of Drug Formulation, Development and Production · Vol. 11, Issue 1, 2024 · pp. 15–27 Read article
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Stability-Indicating Rp-Hplc Development and Validation for the Estimation of Lercanidipine Hydrochloride Content in Tablet Dosage Form
Abstract: A sensitive, stability-revealing, and easy reverse-phase high-performance liquid chromatographic (RP-HPLC) technique was developed and rationalized to be in a position to determine Lercanidipine hydrochloride in ingested dosing preparations. It was separated by chromatography on a C 18 column using a mobile phase containing 10 mM potassium dihydrogen phosphate buffer and methanol (20:80, v/v; pH 4.0) at a flow rate of 1.0 mL/min and UV detected at 258 nm. The retention …
Published in Research & Reviews: A Journal of Drug Formulation, Development and Production · Vol. 13, Issue 1, 2026 · pp. 17–26 Read article
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Analytical Technique Optimization and ICH-Directed Verification of a Reverse Phase HPLC Approach for Quantification of Bupropion Hydrochloride
Abstract: This method was developed for retention time, peak resolution and baseline response using Bupropion Hydrochloride under optimized conditions as validation. The method resulted in two well separated, well defined peaks, a minor peak at 7.5 minutes 12 mV and a main peak at 11.52 minutes 150 mV. At the same time, the baseline has remained stable during the chromatographic run and the fluctuations of noise are very small (0.5 ± …
Published in Research & Reviews: A Journal of Drug Formulation, Development and Production · Vol. 12, Issue 2, 2025 · pp. 47–54 Read article
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Antifungal, Antioxidant Activity and Phytochemical Analysis of Extracts from Vitex rivularis (Lamiaceae)
Abstract: The present study was designed to investigate the biological properties of methanolic extracts of leaves and seeds from Vitexrivularis. Leaves and seeds extracts were prepared by maceration in methanol. Phytochemicals analysis was performed by colorimetric method, chemical reaction method and high-performance liquid chromatography (HPLC). The broth microdilution method was used to evaluate the in vitro activity against three strains of Candida albicans and 17 isolates of two yeast species (Candida …
Published in Research & Reviews: A Journal of Pharmacognosy · Vol. 7, Issue 1, 2020 · pp. 23–48 Read article
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RP-HPLC method for simultaneous determination of Drospirenone and Ethinylestradiol in Bulk and their Pharmaceutical dosage form
Abstract: Oral contraceptive is used to prevent the pregnancy which is contain hormones to block the release of eggs from the ovaries. Most of them contain estrogen and progesterone. Nowadays, usage of hormonal contraceptive is sharply enhancing; around 100 million of women worldwide are taking these drugs. With this drug fertility rate can be supressed as long as they want or plan their activity along with these drugs provide efficacy, reliability, …
Published in Trends in Drug Delivery · Vol. 11, Issue 3, 2024 · pp. 46–67 Read article
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Endogenous and Exogenous Enzyme Effects on Different Breeding Clones of Sweetpotato (Ipomoea batatas Lam.) Roots Starch in Ethanol Production
Abstract: The research described herein compares ethanol yield from the conversion of starch in sweetpotato storage roots via endogenous enzyme hydrolysis to that of exogenous enzyme hydrolysis (diastatic barley malt). White-fleshed roots of sweetpotato (Ipomoea batatas Lam) breeding clones; TU090R0005, TIB4004, TU090020, TU090013, BM8342197-05, BM854219905 and TIB4085 were processed for distillation to determine ethanol concentration. HPLC analysis was used to determine ethanol and residual sugar concentrations and 1H NMR analysis was …
Published in Journal of Alternate Energy Sources & Technologies · Vol. 4, Issue 2, 2013 · pp. 1–7 Read article
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Identification and Quantification of Anti-inflammatory Compound in Michelia champaca
Abstract: Ferulic acid is a ubiquitous phenolic acid present in the plants. This phenolic acid is of low toxicity and can easily be absorbed and metabolized in the human body. Ferulic acid has many physiological functions such as anti-inflammatory, antioxidant, antimicrobial, anti-thrombotic and anti-cancerous activities. In the present study, Ferulic acid was isolated from Michelia champaca extract and quantified through High Performance Liquid Chromatography (HPLC) analysis. Ferulic acid is effective in …
Published in Research and Reviews : A Journal of Life Sciences · Vol. 9, Issue 1, 2019 · pp. 16–23 Read article
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Inclusion of Silver Ions into Modified Cellulosic Fabrics for Durable and Improved Antibacterial Activity
Abstract: Silver ions were added to β-cyclodextrin-grafted organic cotton to create antibacterial fabrics that are more effective and long-lasting. In the presence of sodium hypophosphite, citric acid was used as a cross-linker to graft β-cyclodextrin onto the fabric, cementing the structure for ten minutes at 150°C. The presence of silver ions on the grafted fabric was verified by UV-visible and FTIR spectral studies of β-cyclodextrin, β-cyclodextrin-grafted fabric, ungrafted fabric, silver ions, …
Published in Journal of Thin Films, Coating Science Technology & Application · Vol. 11, Issue 3, 2024 · pp. 39–52 Read article
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Preservation of Cysteine Protease Activity in Oleoresin Extracts of Traditional Spices Aided by Modelling and HPLC Studies
Abstract: In the present study, Zingiber officinale, Allium sativum and Phyllanthus emblica were pretreated with sodium ascorbate and dried using electric tray drier at 65°C. Six different models were applied to the drying experimental data and the best fit model for each sample was determined. The dried samples were then used for the extraction of oleoresin, followed by HPLC analysis. The study elucidates the advantage of sodium ascorbate pretreatment, drying treatment …
Published in Emerging Trends in Chemical Engineering · Vol. 7, Issue 3, 2020 · pp. 11–16 Read article
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Screening Sweetpotato Breeding Clones [Ipomoea batatas (L.) Lam] as a Suitable Potential Source of Feedstock for Bioethanol Production
Abstract: Experiments were conducted to evaluate white and orange fleshed sweetpotatoes as a feedstock for bioethanol production, based on dry matter (DM), extractable starch, amylose-amylopectin ratio, fermentation times and ethanol yields. Ten sweetpotato cultivars viz., W308, TU0002, WS149 05, BM8342119 TU090W009, DMOI158096, DMOI158204, TIB4008, TIB4085 and, Beauregard, were planted in replicated field variety trials. Plants were established from 30 cm long vine cuttings and N was applied (P and K were …
Published in Journal of Alternate Energy Sources & Technologies · Vol. 5, Issue 3, 2014 · pp. 6–12 Read article
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Methods for Removal of Rotenone from Brebra (Millettia ferruginea) Seed, Oil and Defatted Flour under Different Treatment Conditions
Abstract: The main objective was to develop and determine the rotenone removal methods from different samples of defatted flour and refined oil. To remove rotenone, ethanol, pH gradient treatment, cooking and roasting were used and rotenone was determined by HPLC analysis. Most of the rotenone (0.701%) from non-defatted flour is lost during defatting (0.37%) and refinery process of oil (0.02%). From raw seed, 84.1, 85.1 and 97.3% rotenone was removed by …
Published in Research and Reviews : A Journal of Biotechnology · Vol. 3, Issue 3, 2013 · pp. 5–11 Read article
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Modified Cellulosic Fabrics with Eugenol Added for Longer-Lasting and Better Antibacterial Activity
Abstract: Eugenol was added to cyclodextrin-grafted organic cotton to create antibacterial textiles with increased efficacy and durability. Grafting of β-cyclodextrin on fabric was done with citric acid as cross linker in the presence of sodium hypophosphite by fixing at 150oC for 10 min. UV-Visible and FTIR spectral studies of β-Cyclodextrin, β-Cyclodextrin-grafted fabric, ungrafted fabric, eugenol and eugenol –loaded fabric confirmed the inclusion of eugenol on grafted fabric. Eugenol was recovered from …
Published in Journal of Modern Chemistry & Chemical Technology · Vol. 14, Issue 1, 2023 · pp. 14–28 Read article
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Significance of Growth Rate, Acceptability of Fermented Milk and Release of Peptides by Lactic Cultures
Abstract: Ten lactic cultures (S. thermophilus MD2, S. thermophilus MD8, Lc. lactis 009, L. fermentum AI2, L. fermentum AI3, L. fermentum 138, L. plantarum AD29, L. rhamnosus NS4, L. rhamnosus NS6 and E. feacalis ND3) isolated from vegetables and fermented foods were evaluated for their potential to ferment milk, produce good quality curd and ability to release peptides in fermented milk. It was found that S. thermophilus MD8, S. thermophilus MD2 …
Published in Research and Reviews : Journal of Dairy Science and Technology · Vol. 5, Issue 1, 2016 · pp. 31–40 Read article
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Determination of Antioxidant Activity of Phenolic Extract of Grapes
Abstract: The grapes were extracted under various conditions using different solvents. The antioxidant activity of solvent extracts was investigated by DPPH radical scavenging method. Ethanol extract exhibited the highest antioxidant activity compared to the other solvents (BuOH, EtOAc, Me2 Cl2 and pet.ether). There was a correlation between antioxidant activity and total phenol content. HPLC analysis of the extracts showed that gallic and cinnamic acid was the major phenolic compounds in grapes. …
Published in Research & Reviews : Journal of Botany · Vol. 7, Issue 2, 2018 · pp. 17–21 Read article
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Authentication of a Rapid RP-HPLC technique for Quantification of Remogliflozin and Metformin in Medicinal Formulations
Abstract: A new, precise, and stability-assessing advanced liquid chromatography (HPLC) technique for simultaneous quantification of Remogliflozin and Metformin in fixed dose pharmaceutical formulations was designed and verified. Optimization of the technique was carried out on flowing phase composed of ethanenitrile and buffer, under isocratic conditions, the resolution of peak being sharp with retention times of 3.24 min and 4.89 min for Remogliflozin and Metformin, respectively. Technology suitability was confirmed by the …
Published in Trends in Drug Delivery · Vol. 12, Issue 2, 2025 · pp. 70–77 Read article
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Analysis of Anti-psychotic Drug Penfluridol in Human Plasma by HPLC and MS/MS
Abstract: Penfluridol is a new long-acting neuroleptic drug known to be used widely for treating psychotic conditions. An internal standard was employed, which was penfluridol D7 (IS). The analyte and the IS were separated on a Phenomenex Kinetex C18, 5µm 100A, 100*4.6mm column using acetonitrile (40:60, v/v) as the mobile phase and 5 mM ammonium formate as the stationary phase. The retention time of Penfluridol and Penfluridol d7 was set at …
Published in Trends in Drug Delivery · Vol. 9, Issue 2, 2022 · pp. 36–45 Read article