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9 articles for “Extraction Buffer”
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A Rapid and Efficient Protocol of DNA Extraction from Animal Blood and Plant Parts
Abstract: DNA extraction from plant tissues is much more complex in comparison to mammalian tissues and bacterial cells due to the presence of a rigid cell wall. Methods utilized for extraction of DNA from plant tissues can vary depending upon the materials used. It is very essential to obtain the nuclear material (without degradation) by breaking down the cell wall and membrane mechanically. We have used the single protocol for extraction …
Published in Research and Reviews : A Journal of Biotechnology · Vol. 3, Issue 3, 2013 · pp. 1–4 Read article
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Detection of Listeria monocytogenes in Milk and Ice Cream by Polymerase Chain Reaction
Abstract: Abstract The present study was undertaken to standardize PCR assay for detection of L. monocytogenes and Listeriolysin O from livestock foods and compare its efficacy with conventional cultural methods. A set of primer derived from iap gene and other set derived from hlyA gene were used for detection of L. monocytogenes and Listeriolysin O in the PCR assay. The PCR assay was initially standardized by optimizing the concentration of the …
Published in Research and Reviews: A Journal of Microbiology and Virology · Vol. 5, Issue 1, 2015 · pp. 24–35 Read article
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Design of EMG based Control System for Prosthetic Arm
Abstract: Prosthesis, field of bio-mechatronics, is an artificial extension used to replace the function or appearance of a missing limb or lost body part due to injury (traumatic), to correct congenital deficiencies or to supplement defective body parts. A myoelectrically controlled prosthesis detects muscle contractions using electrodes and this electrical activity acts as a signal to activate the prosthesis. In the presented work, the EMG signal is acquired using surface electrodes …
Published in Journal of Electronic Design Technology · Vol. 4, Issue 2, 2013 · pp. 1–6 Read article
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In vitro Testing of the Antibacterial Activity of Root Extracts of Chlorophytum borivilianum against Organisms Causing Postnatal Infections
Abstract: ABSTRACTThe present study was carried out to study the antibacterial activities of root extracts of Chlorophytum borivilianum. The extract was prepared using different solvents like water, Tris-HCl, phosphate buffer, isopropanol, butanol-water and acetone. The antibacterial activity was tested against different bacteria like Staphylococcus aureus, Klebsiella, Proteus, Pseudomonas aeruginosa and Escherichia coli which cause postnatal infections, by agar diffusion method. Zone of inhibition produced by different extracts was tabulated. Butanol-water extract …
Published in Research & Reviews : Journal of Herbal Science · Vol. 1, Issue 3, 2012 · pp. 1–7 Read article
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Anti-cancerous Activity of the Rhizome Extract of Alpinia galanga L. Willd. on the Human Liver Carcinoma Cells
Abstract: The rhizome of Alpinia galanga. L. (Willd). was collected from three places i.e, Udupi Shankara narayana temple surrounding area, Shringeri and Madikeri accessions. About 100 gms of the rhizome was taken, dried and powdered to obtain 1 gm of the extract through methanol as the solvent. 1 gm of the solid extract was subjected for filteration through millipore syringe filter., to this about 6ml of cell based assay buffer and …
Published in Research and Reviews : A Journal of Biotechnology · Vol. 8, Issue 2, 2018 · pp. 1–6 Read article
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Health Disaster Risk Assessment Using GIS and Remote Sensing Techniques: A Case Study of Malaria
Abstract: Malaria, curable and preventable, is a life threatening disease and also can be regarded as a concurrent health disaster. This disease is greatly influenced by environmental factors such as temperature, rainfall, humidity and altitude which affect the survival and longevity of the vector mosquito, and the presence of water bodies which are breeding sites. Advancement in GIS and remote sensing has brought about an improved spatial analysis through the integrated …
Published in Journal of Remote Sensing & GIS · Vol. 7, Issue 2, 2016 · pp. 50–59 Read article
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Polymer Chemistry in Sustained-Release Herbal Composites: Designing a Stable Euphorbia tithymaloides Topical System with Phospholipid and Polymer Matrices
Abstract: Polymer chemistry enables the stabilization and sustained release of herbal actives, critical for topical therapeutic applications. This study explores a novel process for preparing a stable, sustained-release topical composition of Euphorbia tithymaloides, leveraging polymer-based systems. Ethanolic extract (50 mg) of the plant, rich in unstable sugar moieties (e.g., D-ribose) and sterols (e.g., stigmasterol), is encapsulated within a phospholipid matrix of phosphatidylcholine (50–100 mg) and cholesterol (50–100 mg). This lipid-polymer complex, …
Published in Journal of Polymer & Composites · Vol. 13, Issue 6, 2025 · pp. 165–171 Read article
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Design and Analysis of Quantum Dot Solar Cell Using SCAPS-1D Software
Abstract: Compared to conventional solar cells, quantum dot solar cells (QDSCs) have drawn a lot of attention due to their hybrid structure, low production cost, and greater power conversion efficiency. Nevertheless, carrier recombination in the quasi-neutral region (QNR) continues to limit their performance. The electron transport layer (ETL) and hole transport layer (HTL) are two of the layers that are most important in determining the overall efficiency of the device. The …
Published in Journal of Alternate Energy Sources & Technologies · Vol. 17, Issue 1, 2026 · pp. 15–20 Read article
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Formulation and Evaluation of Golden Heart’s Tooth paste of Piper Betel
Abstract: Toothpaste is a multifunctional oral care product composed of abrasives, surfactants, fluoride-based anticaries agents, and pH buffers. Herbal toothpaste formulations have gained significant attention for the treatment of gingivitis, an inflammatory disease of the gums. Generally, toothpaste formulations maintain a pH range of 5–6 to ensure stability and compatibility with the oral environment. Traditional medicinal plants have long been used by communities for oral healthcare due to their antimicrobial and …
Published in Research & Reviews: A Journal of Pharmacognosy · Vol. 13, Issue 2, 2026 · pp. 43–61 Read article