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247 articles for “assay”
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Anti-Tumour and Anti-Bacterial Activity of Some Ethno-Medicinal Plants
Abstract: Background: Every year, millions of people are diagnosed with cancer, leading to death in a majority of the cases. Because of the high death rate associated with cancer and serious side effects of chemotherapy and radiation therapy, many cancer patients seek alternative methods of treatment. Regarding the safety and effectiveness of natural therapies, the researchers are more interested in the use of natural medicine. In the present investigation, the anti-tumour …
Published in Research and Reviews : A Journal of Immunology · Vol. 7, Issue 3, 2017 · pp. 16–22 Read article
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Evaluation of Antimicrobial Activity of Some Medicinal Plants against Most Prevalent Diabetic Wound Pathogens
Abstract: The present research work attempts to evaluate antimicrobial activity of 90% ethanolic extracts of three traditional medicinal plants viz. Terminalia bellirica, Terminalia chebula, Pyhllanthus emblica. Since ethanol is the best solvent for dissolving antimicrobial compound present as active phytoconstituents in plants, these plants were extracted with ethanol. Each extract was tested for its antimicrobial activity against three most prevalent pathogenic microorganism associated with diabetic wounds, viz. Staphylococcus aureus (MTCC3160), Pseudomonas …
Published in Research and Reviews : A Journal of Medical Science and Technology · Vol. 4, Issue 1, 2015 · pp. 13–20 Read article
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Characterization of Laccase Enzyme from Dye Degrading Bacterial Strains Isolated from Natural Sources
Abstract: The continuous growth in globalization and industrialization has increased the environmental pollution and one such major contaminant is various types of synthetic dyes. Microbial decolorization and degradation of these dyes through secretion of various extracellular enzymes is gaining importance owing to their eco-friendly nature. One such enzyme is laccase, a multicopper oxidase enzyme which has the ability to oxidize both phenolic and non-phenolic compounds through reduction of molecular oxygen to …
Published in Research and Reviews: A Journal of Microbiology and Virology · Vol. 9, Issue 1, 2019 · pp. 65–76 Read article
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Comparative Evaluation of Genotoxicity in Peripheral Blood Mononuclear Cells in Oral Precancer and Cancer Patients Using Single Cell Gel Electrophoresis
Abstract: In the present study the comet assay was performed in peripheral mononuclear cells to evaluate DNA damage among tobacco chewers and smokers. The assay provides a quick, accurate and sensitive way to detect various forms of DNA damage in individual cells. The study comprises 50 oral cancer patients, 12 oral submucous fibrosis patients and 10 healthy controls. The length of the comet tail was used to determine the level of …
Published in Research and Reviews: Journal of Oncology and Hematology · Vol. 11, Issue 2, 2022 · pp. 26–34 Read article
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Toxic Potential of Carbaryl on Reproductive and Fertility Parameter in Male Wister Albino Rats
Abstract: This study aims to evaluate the adverse effect of carbaryl on reproductive system of male Wister albino rats. Adult male Wister albino rats were orally administered with 50, 100 and 150 mg/kg body weight of carbaryl for 90 days. Animals from each group were sacrificed after the study period. Sperm count, sperm motility, antioxidant assay (SOD, CAT, LPO, and protein carbonyl), Hormones assay histological analysis and fertility parameters were performed …
Published in Research and Reviews: A Journal of Toxicology · Vol. 5, Issue 2, 2015 · pp. 1–6 Read article
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Effects of Intraperitoneal Administration of ATRA on the Testis of Elastase Induced Emphysematous Rats
Abstract: Emphysema is the pathological manifestation primarily associated with lungs. However, there are evidences of extra-pulmonary manifestations related to emphysema. The inflammation developed during emphysema triggers the release of several inflammatory mediators, which affects the proximal as well as distal organs. The present study was designed to determine the effect of inflammatory spillage on testicular tissue of male Sprague dawley rats; moreover, the main focus was to assess the potential role …
Published in Research and Reviews: A Journal of Toxicology · Vol. 6, Issue 3, 2016 · pp. 1–12 Read article
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In Vivo and In Vitro Antioxidative Efficacy of Naringenin on Cadmium -Induced Toxicity in Rats
Abstract: The aim of the study was to investigate the in vivo circulating antioxidants level such as vitamin C, vitamin E and GSH in Cadmium (Cd) induced toxic rats and the protective efficacy of naringenin via in vitro free radical scavenging assays. In this investigation cadmium chloride (5 mg/kg body weight (b.w) was administered orally (p.o) for 28 days to induce toxicity. Naringenin was pre-administered orally (50 mg/kg body weight) for …
Published in Research and Reviews: A Journal of Toxicology · Vol. 3, Issue 3, 2013 · pp. 9–16 Read article
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Design, Synthesis and Evaluation of Antitubercular Activity of Mannich Bases of Isoniazid-Containing Thiazolidin-4-one Rings
Abstract: Isoniazid (INH), the established antitubercular drug was selected as the lead for the design and development of antitubercular agents by many researchers. 4-thiazolidinones are well known heterocyclic compounds for their spectrum of biological activities such as antibacterial, antifungal [1], anticancer [2], antitubercular, anthelmintic, anti-inflammatory [3], analgesic [4], antithyroid, local anaesthetic, monoamine oxidase inhibition, anti-Toxoplasma gondii activity etc [5]. Modification of thiazolidinones via Mannich reaction to improve biological activity is also …
Published in Research and Reviews: A Journal of Pharmaceutical Science · Vol. 7, Issue 1, 2016 · pp. 1–12 Read article
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A Review about Scope of Traditional Medicinal Plants in a New Drug Discovery
Abstract: ‘AYUSH’ is the most important traditional system of medicine, which has been practised since thousands of long years by our ancestors. AYUSH has a very good healing power for so many problems by using both traditional and natural medicine. The new drug discovery is taking long time to produce its products and thus there is a reduction in number of new drug approvals due to different issues like legal problems …
Published in Research and Reviews : A Journal of Biotechnology · Vol. 7, Issue 2, 2017 · pp. 1–3 Read article
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In vitro Antioxidant Activity and Phytochemical Analysis of Different Polar Solvent Extracts Obtained from Zanthoxylum rhetsa (Roxb) Bark
Abstract: This study was carried out to evaluate the phytochemical and antioxidant effectiveness of methanolic extract and different fractions (n-butanol, ethyl acetate, chloroform and n-hexane) of Z. rhetsa bark. Phytochemical activity of Zanthoxhylum rhetsa (Roxb) bark extract alkaloids, flavonoids, tannins, steroids, saponins and antraquinones was carried out. Antioxidant scavenging enzyme profile assays were done and profiles were developed for validation. The results indicated that bark extract and fractions of Z. rhetsa …
Published in Research and Reviews : A Journal of Life Sciences · Vol. 5, Issue 3, 2015 · pp. 1–7 Read article
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Bacterial Infected Diseases on Fish Clarias Batrachus Treated by Nanoparticle
Abstract: An experiment was designed to investigate the antibacterial activity of two nanoparticles against fish bacterial pathogens Bacillus subtilis, Vibrio harveyi species. Different concentration of nanoparticles was assessed by well diffusion method against an antibacterial activity. The nanoparticles was analysed by MIC and MBC technique. The potential nanoparticle CeO2 which showed maximum antibacterial was also subjected for the time killed assay method. Among the two nanoparticles CeO2 shows maximum activity against …
Published in Journal of Nanoscience, NanoEngineering & Applications · Vol. 8, Issue 3, 2018 · pp. 65–68 Read article
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Green Synthesis of Selenium Nano-metalloid from the extract of Cabbage (Brassica oleracea var. capitata) leaf and its Antimicrobial Activities
Abstract: Green synthesis specifies the creation of nano-size particles (NPs) by a biological process using the reducing properties of metabolites in the plant extracts. The present work reports the use of cabbage (Brassica oleracea var. capitata.) leaf extract as a reducing and stabilizing agent to synthesize selenium nanoparticles (SeNPs) via the green route of synthesis. The as-prepared SeNPs were analyzed by UV–vis spectroscopy, FTIR, DLS, SEM, and EDX. Nano-crystalline SeNPs has …
Published in Journal of Nanoscience, NanoEngineering & Applications · Vol. 9, Issue 1, 2019 · pp. 12–20 Read article
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Transgenic Expression of Stress Tolerance Gene in Sugarcane (Saccharum officinarum L.)
Abstract: AbstractAgrobacterium tumefaciens LBA4404 mediated transformation of sugarcane variety 31 with two plasmids of pB1121 constructs, one containing PsCBL gene and the other containing PsCIPK gene was studied for stress tolerance capability induction. Calli of the variety Isd 31 were infected with both plasmid containing strains using three different infection times of 60, 90 and 120 min in acetosyringone infection medium. In GUS (β-glucuronidase) assay, randomly selected calli from 120 min …
Published in Research & Reviews : Journal of Agricultural Science and Technology · Vol. 6, Issue 2, 2017 · pp. 32–39 Read article
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Isolation, Identification and Characterization of Bdellovibrio sp. from Soil and its Antibiotic Potential against Selected Bacterial Pathogens
Abstract: Bdellovibrio and Like Organisms (BALOs) are the world's smallest Gram-negative, highly motile delta-proteobacterial predators generally found in diverse environmental niches such as soil, seawater, sewage, bio films, freshwater, gastro intestinal tract of animals and in association with plant roots. These small bacteria attack gram negative bacterial strains attaching to the outer membrane and consuming the prey extracellularly (epibiotic predation) or penetrating their periplasm (periplasmic predation). Bdellovibrio exovorus MPR11 (Accession No. …
Published in Research and Reviews : A Journal of Life Sciences · Vol. 9, Issue 1, 2019 · pp. 119–130 Read article
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Improvement of Yield of Bioactive Compounds and Antioxidant Activity of Moringa stenopetala Leaves by Optimizing Ultrasonic-assisted Extraction
Abstract: Ultrasonic-assisted extraction (UAE) parameters were optimized in this study using response surface methodology (RSM) to improve the total phenolic content (TPC), total flavonoid content (TFC), and antioxidant activity assessed using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2’-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) assays of Moringa stenopetala leaves. According to the results, the optimal UAE parameters were extraction time (26 min), ethanol concentration (68%), and solvent-to-sample ratio (42 mL/g). Based on these optimal parameters, …
Published in Research & Reviews : Journal of Food Science & Technology · Vol. 7, Issue 3, 2018 · pp. 1–11 Read article
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Comparative Study on Chloroform and Aqueous Extracts of Mimusops elengi and Its Pharmaceutical Application
Abstract: ABSTRACTPlants are used in modern medicine where they occupy a very significant place as raw material for important drugs. The present study deals with the phytochemical screening, antimicrobial and antioxidant activities of chloroform and aqueous leaf and stem extracts of Mimusops elengi. In antimicrobial studies, for optical density method the chloroform extract showed more inhibition and zone for well diffusion in case of Streptococcus mitis compared to other Gram-positive and …
Published in Research & Reviews : Journal of Herbal Science · Vol. 1, Issue 2, 2012 · pp. 25–32 Read article
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In vitro Anti-oxidant, in silico Anti-inflammatory and Anti-cancer activity of Messua ferrea
Abstract: ABSTRACTMesua ferrea an ethno-medicinally important plant was investigated for in vitro anti-oxidant, in silico anti-inflammatory and anti-cancer properties. Evaluation of in vitro anti-oxidant activity of methanol extract was carried out by DPPH and ABTS and the results were compared with that of standard Rutin. Phytoconstituents of M. ferrea elucidated from literature survey were subjected to in silico molecular docking analysis against anti-inflammatory protein, membrane protein prostaglandin H2synthase-1 and anti-cancer target …
Published in Research & Reviews : Journal of Herbal Science · Vol. 1, Issue 3, 2012 · pp. 8–15 Read article
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Study the Effect of Antioxidant and Antidiabetic Property of the Selective Indian Herbal Plant Extract (A. indicum) on Type II Diabetes Mellitus
Abstract: Abutilon indicum (A. indicum) is a traditional Indian phytomedicine plant used for the treatment of several diseases such as diabetes mellitus, cardiac diseases and anti-inflammatory diseases. The aim of the study was to evaluate whether A. indicum improves insulin sensitivity and determine its active role in bioactive secondary metabolites. Aldose reductase is an enzyme which contains lower affinity towards glucose and helps in converting glucose to sorbitol and slightly catalyzed. …
Published in Research and Reviews : A Journal of Life Sciences · Vol. 9, Issue 1, 2019 · pp. 53–63 Read article
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In Vitro and In Vivo Screening on Anti-diabetic Properties of Hemidesmus indicus in Alloxan Induced Diabetic Rat Model
Abstract: The aim of the present study is to investigate the anti-diabetic potential of Hemidesmus indicus Linn. R. root extract in Alloxan induced diabetic rat. In phytochemical analysis most of the metabolites in the root extract was noticed such as Anthraquinones, flavonoids, phenols, steroids and proteins. In vitro alpha amylase inhibition assay shows significant percentage of inhibition (14.58%, 22.92%, and 45.83%) with increasing concentration of extracts (0.01 ml, 0.1 ml and …
Published in Research & Reviews : Journal of Herbal Science · Vol. 8, Issue 2, 2019 · pp. 1–6 Read article
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Natural Antibodies Against Enteromicrobes and their Toxins for the Treatment of Rheumatoid Arthritis
Abstract: Autoimmune diseases (ADs) are thought to be brought on by the host immune system accidentally attacking and destroying its own tissue. “Molecular mimicry,” which asserts that antibodies against an infectious agent cross-react with a self-antigen possessing an identical or similar antigenic epitope, is a generally recognised theory to explain the pathogenic mechanism of ADs. This theory, however, was probably developed using false antibody assay data that was heavily impacted by …
Published in Research and Reviews : A Journal of Life Sciences · Vol. 12, Issue 3, 2022 · pp. 1–12 Read article