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56 articles for “Chromatographic separations”
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Analytical Techniques Used In Metabolomic Analysis
Abstract: Comprehensive studies regarding metabolomics provides us ample of information about the structural properties and various other parameters pertaining to the molecule under study. Metabolomics is employed for managing long-term illness such as cancer, diabetes. It’s applications also stretch to treating diseases such as tachycardia along with various cardiovascular diseases. It is used to treat inborn errors of metabolism which are found out in infants. These molecules along with processes of …
Published in Research and Reviews: A Journal of Pharmaceutical Science · Vol. 14, Issue 3, 2023 · pp. 52–68 Read article
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Development and Validation of a High Performance Liquid Chromatographic Method for Related Substances of Raloxifene Hydrochloride in Bulk and Pharmaceutical Formulations
Abstract: The development and validation of reversed phase liquid chromatographic method for the determination of the related substances of Raloxifene Hydrochloride (RLH) in bulk and pharmaceutical formulation is describe. This method is simple isocratic for separation of Raloxifene Hydrochloride and related substances from each other in a thirty minutes run time. To the best of our knowledge, no method described previously in the literature has demonstrated resolution of RLH from this …
Published in Trends in Drug Delivery · Vol. 1, Issue 1, 2014 · pp. 16–23 Read article
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Extraction, Isolation, Characterization of Curcumin-I From Curcuminoids
Abstract: The major goal of this study is to extract, isolate, and characterize curcumin-I, a component of curcuminoids. Turmeric, or Curcuma longa Linn., plays a significant role in Ayurvedic medicine. Turmeric, or Curcuma longa Linn, is the popular name for this plant. An array of chemicals with biological activity may be more easily extracted with the help of this seasoning. Some of the compounds at play include curcuminoids and turmerone, although …
Published in Journal of Polymer & Composites · Vol. 10, Issue 4, 2022 Read article
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Bioremediation Potential: Discovering Naphthalene-Degrading Microbes in Iraqi Contaminated Soil
Abstract: A polycyclic aromatic hydrocarbon, napthalene is an environmental contaminant with a slow rate of disintegration. Biological treatment by microbes present in contaminated places is the most important of the several approaches applied to remove it from the environment. At depths of 5 to 10 cm, soil samples were collected from areas polluted by oil near the Al-Dora and Sheikh Omar refineries. Nutrient agar was cultured with 0.1 ml of each …
Published in International Journal of Pathogens · Vol. 1, Issue 2, 2024 · pp. 32–41 Read article
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Development and Validation of RP-HPLC Method for Determination of Thermal Degradation Impurity in Macrolide Immunosuppressant Substance and Drug Product
Abstract: The present study describes a simple and stability-indicating reverse phase high-performance liquid chromatography (RP-HPLC) method for the quantification of the thermal degradation impurities of Tacrolimus (FK506) namely Ally ester rearrangement impurity (Impurity R) in Tacrolimus capsules and drug substances. Successful separation of impurity R from Tacrolimus, its other related substances and its tautomers were achieved on a Zorbax XDB C8 (150×4.6 mm, 5 μm) and detector of UV at 210 …
Published in Research & Reviews: A Journal of Drug Formulation, Development and Production · Vol. 3, Issue 1, 2016 · pp. 14–19 Read article
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Development and Validation of RP-HPLC Method for Estimation Ertugliflozin in Its Bulk and Tablet Dosage Form
Abstract: Background: This study developed and validated a new, sensitive, appropriate, clear, accurate, and robust reversed-phase high-performance liquid chromatography (RP-HPLC) method for determining safinamide in bulk medication and tablet formulations. An HPLC procedure using a UV detector and the Openlab EZchrome workstation programme was used to separate the samples, as well as a Kromasil C18 (250 mm × 4.6 mm i.d.) 5 μm. At a flow rate of 1.0 mL/min, methanol …
Published in Research & Reviews: A Journal of Drug Design & Discovery · Vol. 9, Issue 1, 2022 · pp. 8–20 Read article
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Analytical Technique Optimization and ICH-Directed Verification of a Reverse Phase HPLC Approach for Quantification of Bupropion Hydrochloride
Abstract: This method was developed for retention time, peak resolution and baseline response using Bupropion Hydrochloride under optimized conditions as validation. The method resulted in two well separated, well defined peaks, a minor peak at 7.5 minutes 12 mV and a main peak at 11.52 minutes 150 mV. At the same time, the baseline has remained stable during the chromatographic run and the fluctuations of noise are very small (0.5 ± …
Published in Research & Reviews: A Journal of Drug Formulation, Development and Production · Vol. 12, Issue 2, 2025 · pp. 47–54 Read article
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Validation and Forced Degradation Studies for the Determination of Erlotinib in Bulk and Pharmaceutical Dosage Forms by RP-HPLC
Abstract: A new reverse phase-high performance liquid chromatography (RP-HPLC) have been developed and validated for the determination of Erlotinib in bulk drug and pharmaceutical dosage form. The developed method is rapid, accurate, precise, simple and economical. The separation was carried out using column Hypersil ODS C-18 (150 mm x 4.6 mm 5 μm particle size) in isocratic mode, using mobile phase composition of 0.1% orthophosphoric acid: Acetonitrile in the ratio of …
Published in Research & Reviews: A Journal of Drug Formulation, Development and Production · Vol. 3, Issue 2, 2016 · pp. 44–53 Read article
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Pharmacognostical and Pharmaceutical Analysis of Amavatari Rasa- A Herbo-mineral Compound
Abstract: Amavatari Rasa is a herbo-mineral formulation, composed of processed mercury, sulphar, Terminalia chebula Retz, Terminalia bellerica Roxb., Phyllanthus embilica (Embelica officinalis Gaertn), Plambagozeylenica Linn., Commiphora wightii (Arn) Bhand and Ricinuscommunis Linn. This formulation is routinely practiced in cases of Rheumatoid Arthritis, but preliminary physicochemical and pharmacognostical profiles are not available. Considering this, an attempt has been made to develop this profile. Pharmacognostical study of raw drugs was carried out. Organoleptic …
Published in Research and Reviews: A Journal of Pharmaceutical Science · Vol. 7, Issue 2, 2016 · pp. 6–10 Read article
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Spectroscopic and Chromatographic Methods for Estimation of Voriconazole in Bulk and Pharmaceutical Dosage Forms
Abstract: Rapid, accurate and precise methods have been developed for estimation of voriconazole in bulk and pharmaceutical dosage forms using Ultraviolet (UV) spectroscopy and reverse phase high performance chromatography (RP-HPLC). The λmax selected in UV spectroscopy method was 255 nm and the calibration curve was linear in the concentration range of 2-50 µg/mL with correlation coefficient (r2)>0.99. In RP-HPLC method, the separation was carried out on Kromasil C18 (250 x 4.6mm, …
Published in Research & Reviews: A Journal of Drug Formulation, Development and Production · Vol. 8, Issue 1, 2023 · pp. 33–39 Read article
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Study on the Degradation of Total Petroleum Hydrocarbon (TPH) by Water Hyacinth Co-composts
Abstract: AbstractThis study was carried out to investigate the rate of total petroleum hydrocarbon (TPH) by water hyacinth co-compost at the Teaching and Research Farm, Faculty of Agriculture, Delta State University (DELSU), Asaba Campus. Five (5) kg of soils was collected from the school farm and measured into eighty-four (84) experimental pots and the treatments were arranged in 4×7 factorial experiment in a completely randomized design replicated three (03) times. The …
Published in Research & Reviews : Journal of Agricultural Science and Technology · Vol. 8, Issue 3, 2019 · pp. 20–26 Read article
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HPLC Method Development by Using QBD Approach: An Overview
Abstract: HPLC is a critical analytical method for obtaining medicinal product information. The HPLC method should be capable of separating, detecting, and quantifying a variety of medicines and associated compounds. Validation is the process of determining the performance and qualities of a product or service. In this paper, I assembled HPLC data using their QbD method. It is more efficient than traditional HPLC. This is the most versatile, safe, reliable, and …
Published in Research and Reviews: A Journal of Pharmaceutical Science · Vol. 13, Issue 1, 2022 · pp. 30–35 Read article
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Synthesis, Characterization and Drug Delivery Application of Poly (Acid Fuchsin) Based Triblock Copolymer
Abstract: Synthesis, characterization and its drug delivery application of Poly (acid fuchsin) (PAF) was done under inert atmosphere. The (M0/I0) was maintained at 100. In order to increase the molecular weight and processability, it was block copolymerized with ε-caprolactone (CL) and tetrahydrofuran (THF) separately. The conductivity value of PAF was found to be decreased after the chemical conjugation with poly(ε-caprolctone) (PCL) and poly(tetrahydrofuran) (PTHF) blocks. Thus obtained triblock copolymer was subjected …
Published in Journal of Catalyst & Catalysis · Vol. 3, Issue 2, 2016 · pp. 33–43 Read article
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Preparation of Methyl Esters of Solid Acids from Litsea Consimilis
Abstract: This study focuses on the extraction and characterization of fatty acids from the seed oil of Litsea consimilis and their subsequent conversion into methyl esters. The oil demonstrated high iodine and Reichert-Meissl values, indicating a significant content of unsaturated and short-chain fatty acids. Glyceride analysis revealed that trilaurin constitutes over 90% of the triglycerides, confirmed through melting point analysis and hydrolysis yielding lauric acid as the sole component. The oil …
Published in Journal of Modern Chemistry & Chemical Technology · Vol. 16, Issue 2, 2025 · pp. 129–134 Read article
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Study on Extraction and Purification of Phytochemicals from Eichhornia Crassipes Leaves
Abstract: Eichhornia crassipes is an aquatic plant that belongs to Pontederiaceae family. It is commonly known as water hyacinth. This annoying plant has been a recurring issue that should have been managed. In comparison to other natural plants and fibers, the seeds of these plants may survive up to 28 years at the water’s surface and have developed extremely quick growth properties. The water hyacinth is regarded as the world’s most …
Published in Research and Reviews : A Journal of Biotechnology · Vol. 14, Issue 3, 2024 · pp. 24–28 Read article
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Simultaneous Determination And Stability Indicating Method For Telmisartan, Metoprolol Succinate And Cilnidipine In Tablet Dosage Form.
Abstract: There are several medicinal agents available for antihypertensive activity. Telmisartan, metoprolol and cilnidipine are among them. Quantitative and qualitative analysis plays a pivotal role in safeguarding patients. Here, the estimation of these drugs was performed by using the chromatographic technique RP-HPLC. It was conducted with Phosphate buffer (pH 3.5 ± 0.01): Methanol (50:50 v/v) mobile phase. The system was operated at 1 ml/min. Drug volume to the system was 20μl. …
Published in Research & Reviews: A Journal of Drug Design & Discovery · Vol. 11, Issue 1, 2024 · pp. 32–38 Read article