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56 articles for “protein sequencing”
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Interaction of Sweet Proteins with Human Sweet Taste Receptor
Abstract: There is a dearth of low calorie sweeteners with minimal or no side effects. Natural molecules are obvious choice for they are expected to be easily metabolized. Despite the availability of natural sweet proteins like brazzein, monellin, thatumatin, etc., they are not suitable for hyperlipidemia patients. That necessitates the design of new peptide-based sweeteners. The present work attempts to understand the interaction of sweet proteins with human sweet taste receptor …
Published in Research and Reviews: A Journal of Pharmaceutical Science · Vol. 4, Issue 1, 2013 · pp. 25–30 Read article
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Biochemical and Structural Characterization of Helicobacter pylori Peptide deformylase by Computational Methods
Abstract: Helicobacter pylorus is classified as class one carcinogen in causing gastric cancer. Peptide deformylases (PDFs) present in bacteria removes the formyl group from the N-terminal Methionine of newly synthesized proteins which is necessary in protein maturation. These enzymes have shown as new targets in antibacterial, antimalarial and anticancer drug discovery. Here an insilico technique was initiated to characterize structural and biochemical properties of this protein. Pair wise sequence alignment with …
Published in Research & Reviews: A Journal of Bioinformatics · Vol. 4, Issue 3, 2017 · pp. 22–28 Read article
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Characterization of a Broadly Specific LPS-Neutralizing Single Domain Antibody
Abstract: Gram negative endotoxaemia or sepsis remains a lethal challenge to humans and animals, globally. Single domain antibodies (dAbs) have emerged as therapeutic biologics for challenging antigens such as lipopolysachharide (LPS). The present study characterized a broadly specific LPS-neutralising dAb produced from phage display library from LPS-immunized Indian desert camel, for its sequence validation with mass spectrometry. Also, the dAb clone 26 was subjected to co-crystallisation with LPS, extracted from E. …
Published in Research and Reviews : A Journal of Immunology · Vol. 7, Issue 1, 2017 · pp. 1–7 Read article
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Comparative Genomics of Heat Shock Protein in Pathogenic Bacteria
Abstract: Genome comparison has recently become a common research topic in computer science. The development of computer-assisted mathematics (using products such as Mathematica or Matlab) has helped engineers, mathematicians, and computer scientists to start operating in this domain, and a public collection of case studies and demonstrations is growing, ranging from whole genome comparisons to gene expression analysis. This has increased the introduction of different ideas, including concepts from systems and …
Published in Research & Reviews: A Journal of Bioinformatics · Vol. 1, Issue 2, 2014 · pp. 19–34 Read article
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Antimicrobial Peptides in Fiddler Crabs: Structural Analysis and Potential Applications
Abstract: Crustaceans represent the largest and most ecologically and economically significant group of marine and aquatic arthropods. Their biomass and critical role in ecosystems highlight their importance. Among crustaceans, decapods are frequently used as model organisms in studies of immune responses due to their significant commercial value and the need to mitigate disease outbreaks in shellfish aquaculture. Antimicrobial host-defense peptides (AMPs) are pivotal in metazoan immunity, especially for invertebrates lacking adaptive …
Published in International Journal of Cheminformatics · Vol. 2, Issue 2, 2024 · pp. 26–32 Read article
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A Review on Protein Structure Prediction
Abstract: Protein structure prediction is the inference of the three-dimensional structure of a protein from its amino acid sequence. The prediction of folding and secondary and tertiary structure from its primary structure. Structure prediction is fundamentally different from the inverse problem of protein design. Protein structure prediction is one of the most important goals pursued by bioinformatics and theoretical chemistry. It is highly important in medicine and biotechnology. Every two years, …
Published in Research & Reviews: A Journal of Bioinformatics · Vol. 3, Issue 2, 2016 · pp. 1–7 Read article
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An Introduction to Proteome Bioinformatics
Abstract: For basic and translational research, high-throughput approaches are essential. Recent improvements in proteomics techniques, for example, have allowed biomedical researchers to characterise the proteome of a variety of creatures. Proteome bioinformatics approaches and technologies have greatly aided this extraordinary progress. The study and use of informatics in the realm of proteomics is known as proteome bioinformatics. This chapter presents an overview of the computational strategies, methodologies, and techniques for bioinformatics …
Published in Research and Reviews : Journal of Computational Biology · Vol. 10, Issue 3, 2021 · pp. 7–9 Read article
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Comparison of the Keratinase Gene Sequences of Fast, Slow and Intermediate Feather Degrading Strains of Bacillus licheniformis
Abstract: Feathers are composed of beta keratin which is an insoluble protein resistant to degradation. Bacillus licheniformis has a gene that codes for a keratinase enzyme that hydrolyzes feathers. The DNA sequences of the genes coding for keratinase enzymes of various strains of B. licheniformis were compared. Some strains of the bacteria hydrolyze feathers rapidly, some slowly, and some at an intermediate rate. The mature keratinase enzyme is composed of 274 …
Published in Research and Reviews: A Journal of Microbiology and Virology · Vol. 5, Issue 3, 2015 · pp. 1–9 Read article
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Phylogenetics of Anopheles and Aedes Mosquito Species in View of Ordant-Binding Protein Motifs of Drosophila
Abstract: Various species of mosquitoes were identified, based on the DNA sequences and subjected to multiple sequence alignment considering the motifs as hallmarks and in turn evolved a phylogenetic cladogram. Keywords: Sequence, phylogenetics, motif, anopheles and aedesCite this ArticleDamodaran Divya, Rani Vimala Grace, Sekar T, et al. Phylogenetics of anopheles and Aedes mosquito species in view of ordant-binding protein motifs of drosophila. Research & Reviews: A Journal of Bioinformatics. 2015; 2(3): …
Published in Research & Reviews: A Journal of Bioinformatics · Vol. 2, Issue 3, 2015 · pp. 27–37 Read article
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Comprehensive In silico Analysis of Single Nucleotide Polymorphisms (SNPs) in Human BDNF Gene
Abstract: Since a number of single nucleotide polymorphisms (SNPs) are associated with the BDNF gene mutations involved in different diseases. Hence, it is important to find out the probable functional SNPs before planning a larger population study. So, to look out for the functional nsSNPs (non-synonymous single nucleotide polymorphisms) in BDNF gene, existing data present in the dbSNP database and different bioinformatics tools like SIFT, PolyPhen, nsSNPAnalyzer, F-SNP, I-mutant, Pymol were …
Published in Research and Reviews : A Journal of Life Sciences · Vol. 8, Issue 3, 2018 · pp. 128–139 Read article
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Recombinant Protein Manufacturing Using Actinomycetes as Host Cells
Abstract: Actinobacteria are highly sought-after for use as cell factories or bioreactors in the pharmaceutical, agricultural, industrial, and environmental sectors. The commercial production of these proteins as recombinants and the biochemical and structural characterization of key proteins have been made possible by the genome sequencing of numerous species of actinomycetes. In this regard, better expression vectors that may be used with actinomycetes are required. As discussed in this article, recent developments …
Published in Research & Reviews: A Journal of Bioinformatics · Vol. 9, Issue 4, 2022 · pp. 23–27 Read article
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Computational Approach in Characterization and Structural Perspective of HPSE2 Gene
Abstract: HPSE2 gene is a gene encoded by heparanase2 enzyme in humans. The gene is found to be associated with urofacial syndrome in humans. The gene is found to be located at Chromosome 10:100.22–101 Mb in human and Chromosome 19:42.79–43.39 Mb in mouse. The current work is done to annotate the gene HPSE2 both functionally as well as sequentially. It includes analysis of the mutation at various sites in the sequences …
Published in Research & Reviews: A Journal of Bioinformatics · Vol. 2, Issue 2, 2015 · pp. 18–24 Read article
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AIS-MACA- Z: MACA based Clonal Classifier for Splicing Site, Protein Coding and Promoter Region Identification in Eukaryotes
Abstract: Bioinformatics incorporates information regarding biological data storage, accessing mechanisms and presentation of characteristics within this data. Most of the problems in bioinformatics and be addressed efficiently by computer techniques. This paper aims at building a classifier based on Multiple Attractor Cellular Automata (MACA) which uses fuzzy logic with version Z to predict splicing site, protein coding and promoter region identification in eukaryotes. It is strengthened with an artificial immune system …
Published in Journal of Artificial Intelligence Research & Advances · Vol. 1, Issue 1, 2014 · pp. 1–6 Read article
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An Immuno-informatics Approach for the Design of Subunit Vaccine for Non-human Homolog Proteins of A.aegypti Species
Abstract: Mosquito borne diseases account for a major fraction of vector-borne diseases that account for significant morbidity amongst the population worldwide. The prevalence rate of mosquitoes in the common habitable area is so high that even after the implementation of several eradication programs their disease-causing rate is still quite significant. A.aegypti and A.albopictus are two prominent species of genus Aedes, which transmit viruses causing several infectious diseases such as dengue, zika …
Published in Research & Reviews: A Journal of Bioinformatics · Vol. 9, Issue 3, 2022 · pp. 1–8 Read article
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Visualization of genomic sequence in BRCA1 and BRCA2
Abstract: Heredity is the main reason of breast cancer in women and mutation or genetic variations also another reason for the spread of breast cancer. Heredity and genetic variations are responsible 5%-10%, 30%-50% respectively. There are 90% chances of developing neoplasm by the mutations. The effect of BRCA1 and BRCA2 may leads to the variations in DNA therefore it should be in limited amounts so that there will be minimum changes …
Published in Research and Reviews: A Journal of Pharmacology · Vol. 15, Issue 1, 2025 · pp. 49–55 Read article
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Significance of Butyrophilin Gene in Relation to Milk Constituents in Dairy Animals
Abstract: Abstract Butyrophilin (BTN1A1) has been classified as member of transmembrane proteins of immunoglobulin family. BTN1A1 is a QTL candidate gene having role in milk production and composition (fat) in dairy animals. The gene product BTN1A1 has function during secretion of milk fat and its expression is tissue specific in lactating mammary tissue. Butyrophilin gene constitutes of a more than 40% by weight of total protein and synthesized as a peptide …
Published in Research and Reviews : Journal of Dairy Science and Technology · Vol. 6, Issue 1, 2017 · pp. 24–30 Read article
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Designer Zinc Finger Nuclease: A Versatile Tool for Genome Editing in Animals
Abstract: In this review, is provided an update on the most popular approaches, including zinc finger nuclease (ZFN)-based gene editing by homologous recombination (HR), gene knockout, non-homologous end joining (NHEJ), and gene therapy in mouse. Zinc finger nucleases (ZFNs) are synthetic restriction enzymes evolved by fusing zinc finger that facilitate genome editing by creating a double-stranded break in DNA at a user-specified location. ZFNs are new tools that promise to radically …
Published in Research and Reviews : A Journal of Biotechnology · Vol. 4, Issue 1, 2014 · pp. 24–30 Read article
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Zika Virus E-Protein In Silico Approach and Drug Repurposing
Abstract: The mosquito borne, one of the flavivirus family, called Zika virus is a positive, single stranded RNA. ZIKV infected humans are affected by neurological disorders, birth defects and other small pain, rash etc. To relieve from these disease, the target protein of ZIKV disease is to be determined. Here, the envelope protein is taken as a target to prevent Zika virus entry in to the cells. 3D structure of ZIKV …
Published in Research & Reviews: A Journal of Drug Design & Discovery · Vol. 4, Issue 2, 2017 · pp. 8–16 Read article
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In silico potential drug target identification against 4-aminobutyrate aminotransferase for Huntington diseases
Abstract: Huntington disease, HD is a progressive neurodegenerative disorder. Some excitatory chemicals released like glutamate which causes the cell damage and the neuron die due to over accumulation of the glutamate in Huntington diseases (HD), which is regulated by the GABA also known as gamma- amino butyric acid, it is a major inhibitory neurotransmitter of the nervous system. Here we are targeting the pyridoxal dependent enzyme which degrades the GABA neurotransmitter, …
Published in Research & Reviews: A Journal of Drug Design & Discovery · Vol. 4, Issue 3, 2017 · pp. 1–5 Read article
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New Wine in Old Wineskin: Progressive Development of Genomic Editing in Bombyx mori
Abstract: The Bombyx mori insect emerging from lepidopteran family is exploited as an important remunerative specie, vis-a-vis as a model organism for scientific findings. It is unequivocally a primal insect model in which genomic altering tool was used to establish a gene function in vitro. A significant milestone in Bombyx genetics is evidenced by contemporary advancement in germline transformation and whole-genome sequencing, producing genetically engineered silkworms (new wine in an old …
Published in Research & Reviews : Journal of Agricultural Science and Technology · Vol. 7, Issue 3, 2018 · pp. 17–25 Read article