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247 articles for “assay”
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Antioxidant Activity of Bacopa monnieri, Tagetes, Withania somnifera and Formulation of Polyherbal Hair Oil
Abstract: The purpose of this research is to evaluate the chromatographic antioxidative activity and thin-layer analysis of medicinal plant leaves. Its antioxidant activities were calculated by calculating its radical scavenging DPPH and radical scavenging ABTS and were obtained in 35.08 per cent, 32.12 per cent, 17.22 per cent, for this research Bacopa monnieri and Withania somnifera. AGAR diffusion assay was used to carry out antibacterial extract activity, and its antibacterial effect …
Published in Research & Reviews : Journal of Herbal Science · Vol. 9, Issue 2, 2020 · pp. 15–19 Read article
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Investigation on Plant Heparanase and its Inhibitors
Abstract: Heparanase is an endoglycosidase which cleaves heparan sulfate (HS) and hence participates in degradation and remodelling of extracellular matrix (ECM). It further regulates multiple biological activities that enhance tumour growth, angiogenesis and metastasis. Heparanase expression is enhanced in almost all cancers examined including various carcinomas, sarcomas and haematological malignancies. In the present article, heparanase enzyme in plants and its activity against inhibitors was described. Here the heparanase enzyme and the …
Published in Research and Reviews : A Journal of Life Sciences · Vol. 10, Issue 3, 2020 · pp. 19–27 Read article
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Assessment of Antioxidant and Antimicrobial (Therapeutic) Potentials of Some Medicinally Important Beverages
Abstract: Herbal tea is widely used for its medicinal properties. Here the Tulsi ginger tea and Tulsi lemongrass tea were taken for antimicrobial and antioxidant assays to compare their therapeutic potentials. Different solvents viz water and Methnol: HCl (99:1) were taken to compare the antioxidant and antimicrobial activities. At the same time Ashwagandha and Arjuna Churna was taken to assess their therapeutic/medicinal values by assessing antioxidant and antimicrobial potential against two …
Published in Research & Reviews : Journal of Herbal Science · Vol. 10, Issue 2, 2021 · pp. 8–13 Read article
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Effect of Caffeic Acid on Glutathione Metabolism of MCF-7 and MDA MB-231 Breast Cancer Cells
Abstract: Increasing incidences of breast cancer at alarming rates with treatment strategies having an overall detrimental effect on the patients led to investigating the potential of phytochemicals as anti-tumorigenic agents. Caffeic acid, an antioxidant and anti-tumorigenic phytochemical, has been tested on MCF-7(hormone responsive) and MDA MB-231(hormone-independent) breast cancer cell lines. Alterations in the levels of intracellular glutathione (GSH) in both cell lines due to exposure to the drug are the primary …
Published in Research and Reviews : A Journal of Life Sciences · Vol. 12, Issue 1, 2022 · pp. 14–22 Read article
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Bland-Altman Analysis for Evaluating AHP-based Wildlife Habitat Suitability Models
Abstract: The paper targets in establishing the potential of Bland-Altman analysis integrated with geospatial approach in habitat suitability ecological studies for species conservation. Assessing agreement between two measurement methods can be done by using Bland-Altman plots and calculating limits of agreement and hence compares two assay methods. This method is tried in this study for evaluating suitable habitats for tiger. The study incorporates Geographic Information System – Multi-Criteria Decision Analysis (GIS-MCDA) …
Published in Research & Reviews : Journal of Space Science & Technology · Vol. 4, Issue 2, 2015 · pp. 11–18 Read article
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Advances in Diagnostic Techniques in Veterinary Medicine
Abstract: Diagnostic techniques play a crucial role in veterinary medicine for accurate disease identification, treatment planning, and monitoring of animal health. This review article explores the latest advancements in diagnostic tools and technologies used in veterinary medicine, focusing on imaging modalities, molecular diagnostics, and point-of-care testing. The first section discusses the advancements in imaging modalities, including digital radiography, computed tomography (CT), magnetic resonance imaging (MRI), and ultrasonography. These technologies have revolutionized …
Published in Research and Reviews : Journal of Veterinary Science and Technology · Vol. 12, Issue 1, 2023 · pp. 1–4 Read article
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Chromosomal Aberration and Mutagenicity Toxicological Studies of Beta-Sitosterol Isolated from Ocimum gratissimum (Lamiaceae)
Abstract: Ocimum gratissimum is an important medicinal plant belonging to the Lamiaceae family. Ocimum gratissimum is a small erect or straggling tree or large, woody, climbing shrub found almost throughout India including Andaman and Nicobar Islands. Stem have been used in antifertility treatment in the indigenous system of medicine, and further clinical tests substantiated their efficacy. The root bark contains diketones, fatty matter, rubber, dulcitol, Beta-sitosterol, phlobatannin and glycosidal tannins. Stem …
Published in Research & Reviews: A Journal of Pharmacognosy · Vol. 4, Issue 2, 2017 · pp. 1–6 Read article
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Targeting Cancer through Angiogenesis Inhibition: Prospective of Azole Based Small Molecules
Abstract: Anticancer drug discovery is a major focus area in the pharmaceutical industry and obtaining targeted drug molecules for the malignant tissue is a major hurdle in this process. With the advancement in the knowledge of biological targets, cancer specific molecules such as monoclonal antibodies have been designed and produced in the recent past. However, the cost and effectiveness of these special products are the biggest challenges for cancer treatment. Small …
Published in Research & Reviews: A Journal of Drug Design & Discovery · Vol. 1, Issue 1, 2014 · pp. 13–18 Read article
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Antibacterial Activity of Leaf and Flower Extract of Lablab purpureus against Clinical Isolates of Staphylococcus aureus
Abstract: The present study was undertaken to determine inhibitory activity of leaf and flower extracts of Lablab purpureus against clinical isolates of Staphylococcus aureus. Agar well diffusion assay was performed to determine antibacterial activity. Among extracts, the flower extract has shown marked inhibition of S. aureus isolates. It can be concluded that the leaf and flower extracts possess inhibitory components. The bioactive components present in the plant are to be separated …
Published in Research & Reviews: A Journal of Drug Design & Discovery · Vol. 1, Issue 2, 2014 · pp. 5–7 Read article
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Killer of the Killer—Myeloperoxidase Inhibitor from Siddha System of Medicine
Abstract: Two proprietary siddha medicines such as NiKu plus and S.I.V.A. herbal drops were evaluated for myeloperoxidase (MPO) inhibition by in vitro enzyme assay. The MPO plays an important role in the predisposition of various complications both in diabetes mellitus (DM) and in psoriasis. Both NiKu plus and S.I.V.A. herbal drops showed high inhibitory activity of MPO. The findings suggest that regular use of NiKu and S.I.V.A. is must for patients …
Published in Research and Reviews: A Journal of Pharmacology · Vol. 8, Issue 3, 2018 · pp. 31–34 Read article
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Studies of Antiviral Activity and Cytotoxicity of Sphaeranthus indicus and Coldenia procumbens
Abstract: The presence of drug-resistant Human Immuno+deficiency Virus (HIV) is a major global concern and warrants the development of novel antivirals as alternative and inexpensive therapy. In the current study, previously unreported antiviral activity of aqueous extracts of two indigenous plants used in Ayurveda in India, such as Sphaeranthus indicus and Coldenia procumbens, against replication of HIV-1 (III B) and HIV-2 (Rod) in MT-4 cells was assigned. These extracts tested were …
Published in Research & Reviews: A Journal of Pharmacognosy · Vol. 5, Issue 2, 2018 · pp. 15–20 Read article
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Phytochemical Screening and the Effect of Temperature Treatment on the Extraction Yield of Phenolic Compounds and the Antioxidative Activities of Two Medicinal Species Growing in Algeria: Ammi visnaga and Mentha pulegium
Abstract: The aims of this investigation are the identification of molecules of secondary metabolism and study the impact of two extraction techniques (extraction by boiling water under reflux and maceration in 70% hydroethanolic solution at ambient temperature) on the yield of phenolic compounds and the antioxidative activity on two species widely used in Algeria as medicinal plants; Ammi visnaga and Mentha pulegium. The results show that phytochemical screening indicates the presence …
Published in Research & Reviews: A Journal of Pharmacognosy · Vol. 5, Issue 3, 2018 · pp. 18–25 Read article
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Recent Trends in Drug Repositioning for Discovery of New Anticancer Drugs
Abstract: Drug repositioning, the process of finding new uses of existing drugs, has been gaining popularity in recent years. The availability of several established clinical drug libraries and rapid advances in disease biology, genomics and bioinformatics has accelerated the pace of both activity-based and in silico drug repositioning. Drug repositioning has attracted particular attention from the communities engaged in anticancer drug discovery due to the combination of great demand for new …
Published in Research & Reviews: A Journal of Drug Design & Discovery · Vol. 3, Issue 2, 2016 · pp. 35–38 Read article
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In vitro Cytotoxicity of 17-Oxo-5-androsten-3β-yl esters against Liver, Neuroblastoma and Colon Cancer Cell Lines
Abstract: A tumor is abnormal growth of body tissue and steroidal derivatives represent a distinct class of antineoplastic agents. Positioning of the oxo group on the parental steroid skeleton has significant effect on the antineoplastic activity profile of the compounds. Recent work from our laboratory has also demonstrated the effectiveness of 17-oxo-5-androsten-3β-yl ester derivatives as potential antiproliferative agents and 5-alpha reductase inhibitors. The current study was undertaken to investigate the cytotoxicity …
Published in Research & Reviews: A Journal of Drug Design & Discovery · Vol. 3, Issue 3, 2016 · pp. 23–27 Read article
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Antimicrobial Potential of Methanolic Extract of Barringtonia acutangula (L.) Gaertn Fruits
Abstract: Barringtonia acutangula is a middle-sized plant which is distributed in Meghalaya, Assam, West Bengal, Madhya Pradesh, Bihar and Odisha, used in treatment of various ailments. The present work has investigated the antimicrobial activity of methanol extract of Barringtonia acutangula fruit against six resistance bacterial strains such as Enterococcus faecalis, Staphylococcus aureus, Acinetobacter bumannii (Gram positive) and Citrobacter freundii, Enterobacter aerogenes, Escherichia coli (Gram negative). The antibacterial activity was evaluated by …
Published in Research & Reviews: A Journal of Pharmacognosy · Vol. 3, Issue 3, 2016 · pp. 13–18 Read article
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Anti-Inflammatory Activity of Stem Bark of Homalium Zeylanicum Benth On Wistar Rats
Abstract: Due to the adverse effects of anti-inflammatory and anti-arthritic drugs, which are currently in clinical use, a need arises to develop newer anti-inflammatory and anti-arthritic agents with less side effects. The investigations of the efficacy of plant-based drugs used in the traditional medicine have evidenced great interest owing to their lesser side effects. In this context the present study was conceptualized to evaluate antioxidant and anti-inflammatory activity of Homalium zeylanicum …
Published in Research & Reviews: A Journal of Pharmacognosy · Vol. 4, Issue 1, 2017 · pp. 39–50 Read article
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Antifungal, Antioxidant Activity and Phytochemical Analysis of Extracts from Vitex rivularis (Lamiaceae)
Abstract: The present study was designed to investigate the biological properties of methanolic extracts of leaves and seeds from Vitexrivularis. Leaves and seeds extracts were prepared by maceration in methanol. Phytochemicals analysis was performed by colorimetric method, chemical reaction method and high-performance liquid chromatography (HPLC). The broth microdilution method was used to evaluate the in vitro activity against three strains of Candida albicans and 17 isolates of two yeast species (Candida …
Published in Research & Reviews: A Journal of Pharmacognosy · Vol. 7, Issue 1, 2020 · pp. 23–48 Read article
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Impact of Grape Seed Extract on Liver Function against Cadmium Chloride-Induced Toxicity
Abstract: The present study was designed to evaluate the antioxidant activities of grape seed and to benefit from the impact of grape seed on hepatotoxicity mediated by cadmium chloride (CdCl2). Gas liquid chromatography (GC-MS) was used to assay the chemical profile. A total of 40 male rats (10 rats per group) were divided into four groups: (1) control group, (2) grape seed group: rats treated with grape seed extract (GSE) at …
Published in Research & Reviews: A Journal of Pharmacognosy · Vol. 7, Issue 3, 2020 · pp. 30–39 Read article
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Development and Validation of a UV-Spectrophotometric method for the Estimation of Nintedanib Esylate in Bulk and its Formulation
Abstract: The present work represents a UV spectrophotometric method for the estimation of Nintedanib Esylate in pure and its formulation. Methanol was used as a solvent. Nintedanib Esylate exhibit absorption maxima at 391 nm. The developed method obeyed Beer-Lambert’s law in the concentration range of 3-18 µg/ml having a line equation Y = 0.065x + 0.001 with a correlation coefficient of 0.999. There is no interference from any pharmaceutical excipients. The …
Published in Research and Reviews: A Journal of Pharmaceutical Science · Vol. 9, Issue 2, 2018 · pp. 49–53 Read article
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Plant Mediated Metal Nanoparticles for Wound Healing Activity – A Review
Abstract: The development of nanotechnology, which is playing a part in every discipline subject, is a recent achievement in the science and technology fields. Modern nanotechnology places a high priority on environmentally friendly methods of producing metal nanoparticles. Utilizing plant extract is cost-effective, environmentally friendly, and has less negative side effects. The development of the new discipline of "Nano medicine" is due to the therapy of various chronic non-communicable diseases with …
Published in Research & Reviews: A Journal of Drug Formulation, Development and Production · Vol. 10, Issue 1, 2023 · pp. 1–11 Read article