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83 articles for “clinical isolates”
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Patient Profile and Antimicrobial Susceptibility Testing of Clinically Isolates In E. coli
Abstract: Escherichia coli, commonly known as E. coli, is a prevalent bacterium capable of causing infections in humans. The development of antimicrobial resistance in E. coli poses a significant public health issue. This study aimed to determine the patient profile and antimicrobial susceptibility patterns of clinically isolated E. coli. A retrospective study was carried out on E. coli samples collected from clinical specimens within a hospital environment. Patient profiles, including age, …
Published in Research and Reviews: A Journal of Microbiology and Virology · Vol. 14, Issue 1, 2024 · pp. 32–47 Read article
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Biofilm Forming and Antimicrobial Susceptibility of Clinical Isolates of Staphylococcus Species
Abstract: Staphylococcus species are the most frequent causes of infection and infections on indwelling medical devices, which characteristically involve biofilms. This study observes the biofilm forming abilities and to analyze antimicrobial susceptibility pattern of clinical isolates of Staphylococcus species were found to be biofilm producers by microtitter plate assay method. And to evaluate the antibiofilm property of Moringa oleifera an Indian medicinal plant, known to possess antimicrobial activity. The results demonstrate …
Published in Research and Reviews: A Journal of Microbiology and Virology · Vol. 5, Issue 2, 2015 · pp. 1–4 Read article
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Existence of Integron I and II Genes from Clinical Isolate Staphylococcus aureus and Their Role in Drug Resistance
Abstract: An integron is a gene capture and dissemination system. They have been identified on both genomic and mobile genetic elements which often contain one or more genes that encode antibiotic resistance. They are commonly associated with bacterial genera in the family of Enterobacteriaceae. Currently, there have been recent reports showing the presence of integrons in Staphylococcus species. The aim of the present study was to assess the existanxe of Class …
Published in Research and Reviews: A Journal of Microbiology and Virology · Vol. 4, Issue 3, 2014 · pp. 18–24 Read article
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Antibacterial Activity of Leaf and Flower Extract of Lablab purpureus against Clinical Isolates of Staphylococcus aureus
Abstract: The present study was undertaken to determine inhibitory activity of leaf and flower extracts of Lablab purpureus against clinical isolates of Staphylococcus aureus. Agar well diffusion assay was performed to determine antibacterial activity. Among extracts, the flower extract has shown marked inhibition of S. aureus isolates. It can be concluded that the leaf and flower extracts possess inhibitory components. The bioactive components present in the plant are to be separated …
Published in Research & Reviews: A Journal of Drug Design & Discovery · Vol. 1, Issue 2, 2014 · pp. 5–7 Read article
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Patient Profile and Antimicrobial Susceptibility Testingin Clinical Isolates of Staphylococcus aureus
Abstract: INTRODUCTION: Staphylococcus aureus is a gram-positive bacterium capable of causing various infections, ranging from minor skin infections to serious bloodstream infections. The rise of methicillin-resistant S. aureus (MRSA) strains has made treating these infections more challenging, as MRSA is frequently resistant to several types of antibiotics.. OBJECTIVE: The aim of this study was to investigate the patient profile and antimicrobial susceptibility patterns of clinical isolates of S. aureus in a …
Published in Research and Reviews: A Journal of Microbiology and Virology · Vol. 14, Issue 2, 2024 · pp. 31–38 Read article
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A Study for the Presence of Enterococcal Virulence Factors Gelatinase, Haemolysin among Clinical Isolates in a Tertiary Care Hospital
Abstract: Enterococcus, considered a normal commensal of intestinal tract, is fast emerging pathogen causing serious infection. Despite the increasing importance of Enterococcus as opportunistic pathogens, their virulence factors are still poorly understood. The potential virulence factors of Enterococci include production of enterococcal surface protein (Esp), gelatinase, and haemolysin. Gelatinase- and haemolysin-producing strains of Enterococcus faecalis have been shown to be virulent in animal models of enterococcal infections. This study was undertaken …
Published in Research and Reviews: A Journal of Microbiology and Virology · Vol. 7, Issue 3, 2017 · pp. 14–18 Read article
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Combined Antibacterial Effects of Honeybee (Apis mellifera) and Garlic (Allium sativum) Extracts against Standard and Clinical Pathogenic Bacteria
Abstract: Normal 0 false false false EN-US X-NONE X-NONE Among natural antimicrobial substances, honey and garlic were widely used and investigated separately. But their synergistic antibacterial effect was not studied. The objective of this study was to investigate synergistic antimicrobial effect of combination of honey and garlic against pathogenic bacteria. The antimicrobial activity of mixture of honey and garlic, honey and garlic extract alone against Salmonella NCTC 8385, Listeria monocytogenes (ATCC …
Published in Research and Reviews: A Journal of Microbiology and Virology · Vol. 3, Issue 1, 2013 · pp. 1–8 Read article
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Molecular Diagnosis of Rifampicin Antibiotic Resistance in Mycobacterium Avium Subspecies Paratuberculosis Isolated from Sheep Clinical Samples
Abstract: The present study was conducted to identify parts of the rpoB gene in MAP of Indian sheep isolates. The rpoB gene (Beta sub unit of RNA polymerase) is involved in conferring the rifampicin antibiotic resistance in MAP bacteria. The resistance is due to the presence of mutations in the 81 bp variable region of the rpoB gene including C1367T and T1442C mutations identified by several workers. The study was further …
Published in Research and Reviews: A Journal of Microbiology and Virology · Vol. 12, Issue 3, 2022 · pp. 35–56 Read article
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Multiple Sclerosis: A Brief Overview of the Disease, Its Pharmacotherapy
Abstract: Multiple Sclerosis is an autoimmune disorder mostly affecting individuals aged between 20-40. In this disorder, the optic, vertebral, and central nervous system nerves are primarily impacted. It is a neurological condition that worsens over time and is characterized by inflammation and the breakdown of the sheath that surrounds and shields the nerve fiber. Common symptoms may include muscle weakness, numbness, tingling, fatigue, vertigo, tremor, vision complications, pain, Lhermitte sign, sexual …
Published in International Journal of Brain Sciences · Vol. 1, Issue 1, 2024 · pp. 13–18 Read article
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Diagnostic Modalities Used for Fungal Isolates Confirmed in Clinically Suspected Cases of Otomycosis: A Screening Study
Abstract: Otomycosis is one of the most attending ailed in ENT practice. Fungal infection of external auditory canal is called otomycosis. Discomfort or pain, itching, ear blockage and ear discharge are symptoms of otomycosis. Ear discharge is seen in the cases of bacterial otitis externa (Furuncle or diffuse otitis externa), otomycosis, acute otitis media, chronic otitis media, etc. So to confirm diagnosis of otomycosis, microbiology test of ear discharge is needed. …
Published in Research and Reviews: A Journal of Microbiology and Virology · Vol. 8, Issue 3, 2018 · pp. 58–64 Read article
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Exploring the Potentials of Quercetin and Kaempferol combinations along with Regular Antibiotics for the Effective Management of Methicillin-resistant Staphylococcus aureus (MRSA)
Abstract: The methicillin-resistant S. aureus (MRSA) is a bacterium that offers the challenge to numerous antibiotics which leads to a variety of problems, ranging from skin infections and sepsis to pneumonia. The natural products based management somewhat opened hope towards the betterment of the present era where the combination with currently available medicines has been studied clinically. Antibiotics (cefixime, ceftriaxone, erythromycin, ciprofloxacin, cephradine, methicillin, ampicillin, levofloxacin, and amoxicillin) and flavonoids (quercetin, …
Published in Research and Reviews: A Journal of Microbiology and Virology · Vol. 8, Issue 3, 2018 · pp. 6–9 Read article
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Extended Spectrum Beta-Lactamase Production By Acinetobacter baumannii Isolated From Hospital Environment
Abstract: Extended spectrum β-lactamases production was determined by Double Disc synergy test for twenty one bacterial isolates of Acinetobacter bumannii isolated from the hospital environment. This study investigates the prevalence, genetic mechanisms, and clinical implications of ESBL production by A. baumannii. We employed molecular techniques to identify and characterize ESBL genes in clinical isolates, alongside evaluating their susceptibility profiles against a range of antibiotics. These resistant strains were notably associated with …
Published in International Journal of Antibiotics · Vol. 2, Issue 1, 2025 · pp. 71–78 Read article
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Molecular Characterization of Environmental and Clinical Non Tuberculous Mycobacteria Isolates from Copper Mines and Miners in Zambia
Abstract: Background: The active and ex-miners have high risk factors for Non Tuberculous Mycobacteria (NTM) disease due to the high burden of Human Immunodeficiency Virus (HIV) infection, tuberculosis and silicosis through extensive use of aerosolised water for dust control in the mining environment. The study aimed at the isolation and characterization of nontuberculous mycobacteria from the miners and ex-miners in Zambia. Methods: Sputum samples were collected from 85 miners and ex-miners132 …
Published in Research and Reviews : A Journal of Immunology · Vol. 13, Issue 3, 2023 · pp. 30–39 Read article
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Inhibitory Action of Sodium Tellurite on Uropathogenic Bacteria
Abstract: The objective of the present study was to predict the toxic properties of sodium tellurite against selected uropathogenic bacteria. A group of nine clinical isolates of different uropathogenic bacteria isolated from urine in suspected cases of urinary tracts infection Enterococcus faecalis, Staphylococcus aureus, Acinetobacter baumannii, Citrobacter freundii, Enterobacter aerogenes, Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis and Pseudomonas aeruginosa were collected and utilized for screening tests. The spectrum of toxic properties …
Published in Research and Reviews: A Journal of Microbiology and Virology · Vol. 8, Issue 2, 2018 · pp. 6–10 Read article
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Evaluating Linezolid: A Comprehensive Analysis
Abstract: Treatments for infections caused by Gram-positive bacteria, such as methicillin-resistant staphylococci and VRE, including skin and soft tissue infections, community-acquired pneumonia, and other infections treated with linezolid, an oxazolidinone antimicrobial agent that functions by specifically inhibiting protein synthesis. Even while antibiotic resistance is becoming more frequent in many nations, linezolid resistance among these pathogens is still low, typically less than 1%. Thus, the emergence of resistance in clinical isolates should …
Published in Research & Reviews: A Journal of Drug Design & Discovery · Vol. 11, Issue 2, 2024 · pp. 11–16 Read article
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Comparison of the Effects of Voriconazole and its Nanogel form on Aspergillus Flavus Isolates in Vitro
Abstract: Aspergillus species are one of the most critical environmental fungi and are considered as a severe factor in causing hospital infections of foreign origin. Objective: Aspergillus flavus is a saprophytic and pathogenic fungus and is found all over the world. In clinical isolates, the voriconazole drug effectively affects more than 95% of Aspergillus isolates. These common drugs have limitations for maximum effect in patients, so nanomedicines are designed and manufactured …
Published in Research and Reviews : A Journal of Medical Science and Technology · Vol. 12, Issue 3, 2024 · pp. 14–20 Read article
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Study on Efflux Pump Inhibition Activity of Marine Actinomycetes against Oflaxcin Resistsnt M. tuberculosis
Abstract: Studies were conducted to isolate marine actinomycetes active against oflaxcin resistant M. tuberculosis. Out of 25 actinomycetes isolates designated as ECRMP 4 showed potent antimycobacterial activity against all three tested Mycobacterial strains. Representative bioactive isolate was characterized using phenotypic property and identified as Micropolyspora sp. The zone of inhibition was 20 ± 0.1 mm (62% RIZD) against M. tuberculosis and 18 ± 0.6 mm (100% RIZD) against OFLR M. tuberculosis. …
Published in Research and Reviews : A Journal of Biotechnology · Vol. 4, Issue 2, 2014 · pp. 25–30 Read article
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In vitro Antibacterial Activity of Dietary Spice Trigonella foenum-graecum and Syzygium aromaticum
Abstract: Multi-drug resistant strains of bacteria are broadly distributed in hospitals and are progressively being isolated from community. The aim of the contemporary study was to govern the potential of spices Trigonella foenum-graecum (Fenugreek) and Syzygium aromaticum (Clove) as antibacterial mediators against drug resistant and virulent strains of gram negative pathogens quarantined from urinary pathway infections. Twelve bacterial isolates from urinary tract infections were studied for their ability to produce virulence …
Published in Research & Reviews : Journal of Herbal Science · Vol. 5, Issue 1, 2016 · pp. 6–11 Read article
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A Method of Universal Primer Design for The Detection of Diverged CTX-M Beta-Lactamases in Multi-Drug Resistant Superbugs
Abstract: Complexity of many diverged β-lactamases in common multi-drug resistant bacteria has reached very high level. We found >40% of common bacteria in Kolkata water bodies were ampicillin resistant and reported clinical isolates were >95% ampicillin resistant worldwide. More than twenty unique sequence classes of β-lactamases were known to cause MDR inactivating penicillin, cephalosporin and carbapenem drugs. Further, there are other diverged MDR genes like drug efflux genes (acrAB, mexAB-EF, tetA), …
Published in Research and Reviews : A Journal of Biotechnology · Vol. 9, Issue 2, 2019 · pp. 1–10 Read article
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Improvised Rapid Antibiotic Sensitivity Test by Disc Dispersion Method
Abstract: Multidrug resistance is the one of vital issue globally, there are several causes for multidrug resistance, like improper utilization of antibiotics and selection of suitable antibiotics are the one of the predominant reasons for multidrug resistance gaining. To enable physician to select the suitable antibiotics, appropriate antibiotic sensitivity shall be performed in shortest time period. A method has been developed to get the antibiotic sensitivity test in 2–3 hours for …
Published in Research and Reviews: A Journal of Microbiology and Virology · Vol. 8, Issue 3, 2018 · pp. 44–57 Read article