Chemically Well-Defined Extender for Preservation of Black Bengal Buck Semen

M. Karunakaran; Pangdun Konyak; Ajoy Mandal; M. Mondal; C. Bhakat; D.K. Mandal; S.K. Das; M.K. Ghosh

doi:10.37591/rrjodst.v6i2.501

Abstract

This experiment was carried with the aim to prepare a chemically well defined semen extender with soybean lecithin (SL) for cryopreservation of black Bengal buck semen. Semen samples were collected from five black Bengal bucks. The semen samples were extended in Tris buffer with 5% glycerol containing either 15% egg yolk (control group) or SL at different concentrations (1, 1.5 and 2% SL). The semen samples were cooled gradually to 5°C and equilibrated for 3 h at 5°C and frozen in static liquid nitrogen vapor and stored in liquid nitrogen. Semen samples were evaluated after initial dilution, after completion of equilibration and after freeze thawing for sperm motility. Semen sample preserved in extender containing 1% SL was able to maintain sperm motility at par with extender containing egg yolk. However, reduction in motility was observed as the concentration of soybean lecithin increased above 1% level. Artificial insemination of goats with frozen semen preserved with 1% SL gave 51.95% kidding rate. It is concluded that an extender containing soybean lecithin @ 1% can be used as a chemically well defined extender for cryopreservation of black Bengal buck semen. Keywords: Buck, semen, preservation, soybean lecithin, motilityCite this ArticleKarunakaran M, Pangdun Konyak, Ajoy Mandal, et al. Chemically Well-Defined Extender for Preservation of Black Bengal Buck Semen. Research & Reviews: Journal of Dairy Science and Technology. 2017; 6(2): 7–10p.