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Research and Reviews : A Journal of Biotechnology Original Research

Characterization of Cellulose Producing Bacterial Isolates from Rotten Fruits

  1. Samriddh Srivastava Department of Biotechnology, Jaypee Institute of Information Technology
  2. Garima Mathur Department of Biotechnology, Jaypee Institute of Information Technology

Abstract

Cellulose, the most abundant natural polymer, is predominantly sourced from plant wood but can also be synthesized by certain bacteria in the form of bio-cellulose. The potential applications of bio-cellulose are extensive, particularly in the biomedical field for tissue engineering, drug delivery, and more. The distinct properties and purity of bacterial cellulose, in contrast to plant-derived cellulose, underscore its importance and drive further research in the area. This study focuses on bacterial strains isolated from spoiled fruits to explore their cellulose-producing capabilities for broader industrial applications. Spoiled fruits were collected from Noida, India, and bacterial isolation was carried out from samples of apples, grapes, bananas, as well as coconut. The isolated strains were cultured in Hestrin- Schramm medium and subsequently identified using morphological, biochemical, and 16S rRNA sequencing techniques. The findings obtained from this study revealed variations in cellulose production among the isolated strains. Out of the ten bacterial isolates obtained, each fruit type contributed differently to the total number of isolates. Through the identification process, several isolates were classified as belonging to the Komagataeibacter species. Specific isolates such as BC-G1 and BC-C1, demonstrating early promising cellulose production, were further characterized via 16S rRNA sequencing, confirming their high similarity to known Komagataeibacter saccharivorans strains. Additionally, this study further utilized FTIR spectroscopy to examine the chemical properties of the produced cellulose, providing insights into its structural characteristics and potential applications. The findings from this study highlight the diversity of cellulose-producing bacterial strains isolated from spoiled fruits and their potential for industrial utilization in various sectors, including biomedicine and materials science.

Keywords

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