STM Journals Peer-reviewed research from Consortium e-Learning Network

Journal of Polymer & Composites Original Research Special issue

Development of Biodegradable Poly (Lactic-Co-Glycolic Acid) (PLGA) Nanoparticles for Sustained Release of Insulin

  1. Amit Kumar Singh Department of Pharmaceutics, United Institute of Pharmacy, A-31/1, UPSIDC Industrial Area, Naini, Prayagraj
  2. Anuradha Singh Department of Pharmacology, SBS Group of Institution, SBS College of Pharmacy, Malwan. Fatehpur
  3. Raosaheb Y. Ghegade Department of Pharmacognosy, Gokhale Education Society's, Sir Dr. M. S. Gosavi College of Pharmaceutical Education & Research, Nashik
  4. Shubham Sharma Department of Pharmaceutics, School of Pharmacy, Mangalayatan University, Aligarh
  5. Chetna Devkar Department of Computer Science & Engineering, National Institute of Technology Bhopal
  6. Kavitha C Department of Chemistry, Bannari Amman Institute of Technology, Sathyamangalam, Erode
  7. Manisha S Nangude Department of Pharmacognosy, Shivajirao S Jondhle College of Pharmacy, Mumbai University, Asangaon, Thane
  8. Anagha Amit Sarvadnya Department of Pharmacognosy, SMES'S Mahavir Institute of Pharmacy, Nashik
  9. Mrinal Kanti Bhoumik Manufacturing Science and Technology, Jubilant Cadista Pharmaceutical Inc, 790 Township Line Road, Yardley

Abstract

This research outlines the creation of biodegradable composite nanoparticles made from poly (lactic-co-glycolic acid) (PLGA) designed for the sustained release of insulin. The emphasis is on the interactions between the polymer and the drug at the interface, along with the dynamics of the release matrix. The in vitro release kinetics, surface morphology, encapsulation efficiency, particle size, and polydispersity of insulin-loaded PLGA nanoparticles were analyzed following their production via a double emulsion solvent evaporation method. The nanoparticles composed of the enhanced polymer composite exhibited remarkable colloidal stability, characterized by a narrow polydispersity index of 0.168 ± 0.021 and an average particle size of 185.4 ± 12.6 nm. The encapsulation efficiency was determined to be 78.6 ± 3.2%, while the drug loading was found to be 9.4 ± 0.7%. The establishment of a stable polymer composite system was evidenced by scanning electron microscopy, which revealed smooth, spherical nanoparticles. Insulin was uniformly distributed inside the PLGA polymer matrix without chemical incompatibility, as verified by Fourier transform infrared spectroscopy and differential scanning calorimetry. The first burst release of 12.3 ± 1.8% transpired within the first 6 hours of in vitro release studies, followed by a sustained insulin release of 72.5 ± 3.6% over 120 hours, primarily attributed to diffusion and gradual degradation of the polymer matrix. The produced polymer composite nanoparticles maintained the structural integrity of insulin, exhibiting remarkable storage stability for three months at 4 °C. In conclusion, the results underscore the significance of polymer composite engineering in sophisticated controlled-release drug delivery systems, indicating that PLGA-based polymer composite nanoparticles provide a biodegradable medium for prolonged protein delivery.

Keywords

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