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6 articles for “nucleic acid amplification”
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Evaluation of Nucleic Acid Amplification Method in Diagnosing Enteric Fever in Children
Abstract: The comparison of three detection methods (Widal test, Typhi dot and Nested PCR) for diagnosis of typhoid fever was done in laboratory where gold standard was either Widal positive or Typhi dot positive or both positive. A nested polymerase chain reaction (PCR) specific for Salmonella enteric serovar typhi was used for the detection of the pathogen in blood samples. Total 52 patients with clinical suspicion of typhoid fever were recruited …
Published in Research and Reviews: A Journal of Microbiology and Virology · Vol. 3, Issue 1, 2013 · pp. 9–15 Read article
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Cartridge-Based Nucleic Acid Amplification for Rapid Tuberculosis Diagnosis
Abstract: In terms of public health, tuberculosis continues to have a significant impact on people's lives. The process of determining the underlying cause of a sickness or condition in a patient is known as clinical diagnosis. It entails the use of numerous diagnostic methods, including physical examinations, laboratory testing, and imaging procedures for medical purposes. Smear microscopy, which are frequently utilized, have poor sensitivity and lengthy turnaround times, respectively. An innovative, …
Published in International Journal of Pathogens · Vol. 1, Issue 1, 2024 · pp. 43–48 Read article
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Prevalence of HIV and TB Co-infection: Mumbai Scenario
Abstract: HIV, being the most powerful risk factor, has made the reappearance and progression of tuberculosis easier. The partnership of HIV and TB is a major health problem in both developed and developing countries. The present study was undertaken in Mumbai to study the prevalence of HIV and TB coinfection in patients who visited the tertiary care centers in the city. Both HIV and TB were diagnosed using nucleic acid amplification …
Published in Research and Reviews : A Journal of Biotechnology · Vol. 3, Issue 2, 2013 · pp. 26–32 Read article
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Loop-Mediated Isothermal Amplification Coupled Lateral Flow Assay to Detect Nucleic Acids in Clinical Samples for Brucella Species at Point of Care and Resource-Limited
Abstract: Brucellosis is a most common and neglected zoonotic disease caused by Brucella species. Brucellosis is a major public health challenge as both animals and humans are affected. Most of the Brucella diagnostic tools are focused around serological assays though these assays cannot detect active pathogenesis. Loop-mediated isothermal amplification (LAMP) coupled with lateral flow assay (LFA) was developed in the present study to detect IS711 gene of Brucella species in the …
Published in Research and Reviews : A Journal of Life Sciences · Vol. 10, Issue 2, 2020 · pp. 35–42 Read article
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Polymeric Microfluidic Chips for Rapid Point-of-Care Diagnostic Testing
Abstract: Polymeric microfluidic chips have emerged as promising platforms for rapid point-of-care (POC) diagnostic testing by integrating sample preparation, fluid manipulation, biochemical reactions, and signal detection within compact and potentially low-cost devices. Their compatibility with materials such as polydimethylsiloxane (PDMS), polymethyl methacrylate (PMMA), cyclic olefin copolymer (COC), polystyrene (PS), polycarbonate (PC), and polymer–paper composites provide considerable flexibility in device design and manufacturing. Advances in soft lithography, injection moulding, hot embossing, laser …
Published in Journal of Polymer & Composites · Vol. 14, Issue 4, 2026 Read article
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Pathogenesis, Treatment and Molecular Mechanisms for Development of Multidrug-resistant (MDR) TB as well as Identification of MDR Mycobacterium Tuberculosis
Abstract: Normal 0 false false false EN-US X-NONE X-NONE Tuberculosis (TB) is a medical, social, and economic disaster of huge magnitude in the world. Prevalence of TB has increased due to poverty, population growth, migration and a high number of TB cases in HIV positive individuals. High prevalence rate of M. tuberculosis strains with multidrug resistance (MDR) is a serious problem as global emergency. Strains of M. tuberculosis resistant to both …
Published in Research and Reviews: A Journal of Microbiology and Virology · Vol. 3, Issue 1, 2013 · pp. 16–38 Read article