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5 articles for “bovine serum albumin”
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Application of Aqueous Two-Phase Systems in the Extraction of Bovine Serum Albumin
Abstract: Protein partitioning in an aqueous two-phase system (ATPS) has been found to be a powerful method for extracting and separating mixtures of biomolecules. In ATPS, many factors influence the partition coefficient K (which is the ratio of protein concentration in the top phase to that in the bottom phase) and the recovery percent (Rec%). Two systems of ATPS were used: first, polyethylene glycol (PEG4000)/sodium phosphate, and second, PEG4000/dextran. The behavior …
Published in Journal of Polymer & Composites · Vol. 11, Issue 1, 2023 · pp. 41–50 Read article
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Molecular Cloning, Expression, Purification, and Characterization of Methionine Aminopeptidase
Abstract: MAP enzyme can efficiently remove N-terminal methionine from recombinant proteins. In the present study, MAP gene from genomic DNA of Escherichia coli was amplified, cloned into pET 11b vector and transformed into E.coli DH5a cloning strain to confirm the insertion of MAP gene, then plasmid was transformed into E. coli BL21 (DE3) expression strain. The protein was eluted with 25 mM NaCl and showed a purity of ~ 95% which …
Published in Research and Reviews: A Journal of Microbiology and Virology · Vol. 2, Issue 2, 2012 · pp. 12–24 Read article
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High Pressure Processing and Its Impact on Milk Proteins: A Review
Abstract: High pressure processing (HPP) is a non-thermal or cold pasteurization technique topreserve various food products. High pressure in range of 100–1000 MPa affects size,shape and conformation of various milk proteins. Casein micelles having primaryconformation are not affected under low pressure, but disintegrate at high pressure(300 MPa). β-lactoglobulin (β-lg) is one of the most pressure-sensitive proteins and α-lactalbumin (α-la) is one of the most pressure resistant. Bovine serum albumin,lactoferrin and immunoglobulins …
Published in Research and Reviews : Journal of Dairy Science and Technology · Vol. 2, Issue 1, 2013 · pp. 12–20 Read article
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Phyto-chemical Screening, In Vitro Anti-arthritic Study, Formulation and Evaluation of Ethanolic Extract of Helicteres Isora Fruits
Abstract: The present investigation was aimed at exploring the anti-arthritic activity of ethanolic extract of Helicteres isora fruits, using Bovine serum albumin (BSA) and Egg albumin denaturation method. The extract was subjected to preliminary phyto-chemical screening in order to find out the major phyto-constituents present in it. Various concentrations of the extract (100, 250, and 1000 μg/ml) were prepared and the percentage inhibition of protein denaturation was determined. Diclofenac sodium (100, …
Published in Research and Reviews: A Journal of Pharmacology · Vol. 10, Issue 1, 2020 · pp. 34–40 Read article
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Synthesis, Characterization and Biological Evaluation of Some Novel Phenazine Derivatives
Abstract: Novel phenazine analogues (PZ1‑PZ6) were synthesized, characterized by the spectral analysis and screened for anti-arthritis activity by bovin serum and egg albumin protein denaturation method. The synthetic route involves the equimolar mixture of ortho-phenylenediamine and ammonium persulphate which were dissolved in 1 M HCl to give 2,3-diaminophenazine(I). Different substituted aromatic aldehydes were added to the 2,3-diaminophenazine(I) and dissolved in methanol. The reaction mixture was stirred for 1‑2 h and then …
Published in Research & Reviews: A Journal of Drug Design & Discovery · Vol. 6, Issue 3, 2019 · pp. 31–37 Read article