Search
6 articles for “Transgenic plants”
-
Edible Transgenic Plant Vaccines – An Overview and Update
Abstract: Normal 0 false false false EN-IN X-NONE X-NONE Plants are used as bioreactors to express antigenic molecules to be administered as vaccines. Edible transgenic plant vaccines (ETPV) are plant materials (recombinant protein subunit vaccines), and the edible plant tissue functions as an oral vaccine. ETPV are mucosal targeted vaccines where stimulation of both systematic and mucosal immune network takes place and these diminish the utilization of conventional animal or bacterial …
Published in Research and Reviews : A Journal of Biotechnology · Vol. 1, Issue 2, 2011 · pp. 6–19 Read article
-
Antibody Pharming in Transgenic Plants: An Overview
Abstract: Antibodies (Abs) are secreted large-sized glycoprotein products of terminally differentiated B cells in vertebrate species and since their discovery during 1890s, they have been widely used for diagnosis, prevention and treatment of diseases, epidemiology and life-science research. They have been traditionally produced in large amounts by repeated immunization of animals. Various techniques for production of full-length and fragments of antibody that have been introduced later are: (i). hybridoma technology during …
Published in Research and Reviews : A Journal of Immunology · Vol. 3, Issue 3, 2013 · pp. 1–10 Read article
-
Construction and Development of pSB111 Bar Plasmid Vector for Activation Tagging
Abstract: To get desired traits and to know the precise expression patterns of genes in the plant, Agrobacterium tumefaciens has been widely employed in the generation of transgenic plants using plasmid vectors. Many methods are come forward to establish the gene expression of unknown genes but in these techniques gene alteration isthe main drawback orsometimesit islethal to organisms also, to overcome these problems activator tag method developed to know the specific …
Published in Research & Reviews : Journal of Botany · pp. 1–6 Read article
-
Transgenic Expression of Stress Tolerance Gene in Sugarcane (Saccharum officinarum L.)
Abstract: AbstractAgrobacterium tumefaciens LBA4404 mediated transformation of sugarcane variety 31 with two plasmids of pB1121 constructs, one containing PsCBL gene and the other containing PsCIPK gene was studied for stress tolerance capability induction. Calli of the variety Isd 31 were infected with both plasmid containing strains using three different infection times of 60, 90 and 120 min in acetosyringone infection medium. In GUS (β-glucuronidase) assay, randomly selected calli from 120 min …
Published in Research & Reviews : Journal of Agricultural Science and Technology · Vol. 6, Issue 2, 2017 · pp. 32–39 Read article
-
Improvement of Crop Plants through in vitro Techniques
Abstract: There has been a great demand for high-yielding, disease-free planting stock for agricultural, forestry, plantation and horticulture crops with improved quality. In vitro tissue culture techniques has emerged as a viable tool for the improvement of crops. The various in vitro techniques utilized for crop improvement includes: micropropagation which helps in large scale production, meristem culture which can effectively remove pathogens from plants, embryo culture can overcome the post-zygotic failure …
Published in Research & Reviews : Journal of Botany · Vol. 11, Issue 2, 2022 · pp. 12–24 Read article
-
Conventional and Molecular Breeding for Development of Drought Tolerant Maize Cultivars
Abstract: AbstractIn crop plants, possibility of attaining higher production level depends on the magnitude of genetic potential of germplasm and environmental conditions. Drought being the major abiotic stress, adversely affects the growth and development ultimately economic yield of maize crop in variety of ways. The precise knowledge of drought sensitive crop stages, drought tolerance imparting traits, screening techniques and breeding strategies are having the fundamental prerequisite for development of drought tolerant …
Published in Research and Reviews : Journal of Crop science and Technology · Vol. 4, Issue 1, 2015 · pp. 1–3 Read article