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40 articles for “Tissue culture”
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Natural Polyamine-capped Silver Nanoparticles used as a Decontaminant in Plant Tissue Culture
Abstract: Silver nanomaterials have been extensively practiced as an antimicrobial agent in water treatment, burnt treatment, wound dressings, etc. But the use of nanosilver as a decontaminant in plant tissue system has not yet been a popular practice in the field of agronomy. However, practically there are scanty or no reports on the use of nanosilver as a decontaminant in plant tissue culture methods. In the present work, different types of …
Published in Nano Trends – A Journal of Nano Technology & Its Applications · Vol. 17, Issue 2, 2015 · pp. 31–36 Read article
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Analysis of Genetic Basis for Morphological Variations in Normal and Tissue Cultured Plants of Andrographis paniculata in Association with or Without Arbuscular Mycorrhizal Fungi using AFLP Markers
Abstract: Andrographis paniculata Nees, a member of Acanthaceae, is one of the important medicinal herbs. The active principle, Andrographolide esters has a paramount importance in pharmaceutics. Morphological variants have been reported both in natural populations collected from various geographical areas and micropropagated plants raised through tissue culture that need to be analyzed with proper molecular tools. In order to facilitate reasoned scientific decisions on its management, conservation and prepare for a …
Published in Research & Reviews : Journal of Botany · Vol. 8, Issue 1, 2019 · pp. 1–11 Read article
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Tissue Culture Studies on Bacopa monnieri: A Review
Abstract: AbstractAs the plants goes endangered due to overexploitation and anthropogenic activities, there is necessity to develop a technique to conserve them—micropropagation is one of them. To conserve Bacopa—a high economic value contained medicinal plant—different tissue culture modes are used. The growth agents were used at different concentration and high growth of regeneration was found. This review highlights the recent development and achievements made for the multiple shoots regenerated from different …
Published in Research & Reviews : Journal of Botany · Vol. 3, Issue 3, 2014 · pp. 27–39 Read article
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A Rapid in vitro Propagation of Bambusa balcooa by Plant Tissue Culture Technique
Abstract: Bambusa balcooa is a common plant grown in Bihar, Orissa, Jharkhand and Uttarakhand states of India having good cultural importance. The present experimentation on nodal explants of B. balcooa on MS media supplement under particular culture conditions have shown shunted growth in 30 days. Shoot tip explants of B. balcooa seedlings produced multiple shoots on MS medium supplemented with different plant growth regulators (PGRs) individually and in combination. Shoot tip …
Published in Research and Reviews : A Journal of Biotechnology · Vol. 8, Issue 1, 2018 · pp. 27–31 Read article
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Differential Rooting Pattern Observed in Tissue Culture Regenerated Plantlets from Different Explants of Jatropha curcas L.
Abstract: Normal 0 false false false EN-US X-NONE X-NONE MicrosoftInternetExplorer4 In an attempt to optimize an in vitro regeneration protocol, both direct and indirect, from different explants of Jatropha curcas L., different rooting patterns were observed from the regenerated plantlets in different concentrations of auxins. The rooting pattern was classified into four types based on their morphology as hairy fibrous roots, tap root-type, thin long roots, and thick short roots. Observations …
Published in Research and Reviews : A Journal of Biotechnology · Vol. 3, Issue 1, 2013 · pp. 1–5 Read article
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Improvement of Crop Plants through in vitro Techniques
Abstract: There has been a great demand for high-yielding, disease-free planting stock for agricultural, forestry, plantation and horticulture crops with improved quality. In vitro tissue culture techniques has emerged as a viable tool for the improvement of crops. The various in vitro techniques utilized for crop improvement includes: micropropagation which helps in large scale production, meristem culture which can effectively remove pathogens from plants, embryo culture can overcome the post-zygotic failure …
Published in Research & Reviews : Journal of Botany · Vol. 11, Issue 2, 2022 · pp. 12–24 Read article
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Enhancement of Some Pharmaceutically Important Secondary Metabolites By in vitro Techniques
Abstract: AbstractSecondary metabolites are economically important as drugs, dyes and pigments, pesticides, and food additives. The evolving commercial importance of secondary metabolites has in recent years resulted in a great interest in mechanism of secondary metabolism, especially in the production of bioactive plant metabolites by means of tissue culture technology. Plant cell and tissue culture technologies can be established for both multiplication and extraction of secondary metabolites. Strain improvement, methods for …
Published in Research & Reviews : Journal of Herbal Science · Vol. 3, Issue 1, 2014 · pp. 6–14 Read article
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Biological Property of Lung Clone Cell Strain “Pyong-09-2”
Abstract: We cultivated lung clone cell strain, “Pyong-09-2” whose genetic characters are proper from fetal lung tissue-originated lung cell strain and found out their biological property. Lung clone cell strain, “Pyong-09-2” formed monolayer in which polygonic epithelioid cells are arranged uniformly, like original cell strain “Pyong-09-2” and their growth rate and activity are highest in medium containing 1% serum in 24～84 h of culture. Their viral infectious titer, plaque forming ability, …
Published in Research & Reviews: A Journal of Bioinformatics · Vol. 8, Issue 3, 2021 · pp. 29–34 Read article
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Hydroponics Culture Regulated Metabolic Variations in Plants of Medicinal Value
Abstract: The current review chiefly brings to light the importance of alternative cultivation strategies for medicinal plants about the effective enhancement of the bioactive substances. The fast-paced society is favoring the need for its cost-effective cultivation and studying the effects thereafter. The mis-steps while collecting the plant from the wild lead to identification issues, genetic and phenotypic variations, variable bioactive substances/secondary metabolites, and toxic compounds. So, cultivation happening in controlled environments, …
Published in Research & Reviews : Journal of Herbal Science · Vol. 13, Issue 2, 2024 · pp. 14–20 Read article
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A Review on Biotechnological Aspects of Conservation and Enhancements of Indian Snakeroot (Rauwolfia serpentina)
Abstract: Indian snakeroot (Rauwolfia serpentina) is one of the most important medicinal plants found in the Indian subcontinent, used in the treatment of various types of diseases. Recently it has been considered as a critically endangered plant by the International Union of the Conservation of Nature and Natural Resources (IUCN) due to its heavy exploitation in the past. The plant has a high demand in the international market due to its …
Published in Research & Reviews: A Journal of Drug Design & Discovery · Vol. 9, Issue 2, 2022 · pp. 30–38 Read article
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CHARACTERIZATION, VIRULENCE TESTING AND ANTIFUNGAL SUSCEPTIBILITY PATTERN OF VARIOUS CANDIDA SPECIES AT TERTIARY CARE CENTRE, AMRITSAR
Abstract: IntroductionSerious fungal infections particularly Candida infection have increased in recent years as a consequence of increased immunosuppression associated with HIV infection, organ and tissue transplant and aggressive treatment for malignancies and autoimmune diseases. Present study was conducted to Characterize various Candida species isolated from various clinical samples, their virulence testing by biofilm production and antifungal susceptibility pattern.Settings and Design:A prospective observational study was conducted from September 2014 to June 2016 …
Published in Research and Reviews: A Journal of Microbiology and Virology · Vol. 9, Issue 2, 2019 · pp. 1–5 Read article
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A Review On Evaluation Of Abiotic Stress Signals On Production Of Gymnemic Acid In In Vitro Cultures Of Gymnema Sylvestre R.Br
Abstract: The demand and market value of medicinal plants increases day by day. The raw materials for herbal medicine industry came from wild collections. Due to intense demand, natural populations are under stress and many species of medicinal plants became vulnerable. To overcome the issue new methods to conserve and propagate medicinal plants on large scale. In vitro propagation techniques are effective for the multiplication and conservation. Advantages like availability of …
Published in Research & Reviews : Journal of Botany · Vol. 12, Issue 2, 2023 · pp. 28–35 Read article
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The study on the isolation, culture, differentiation and transplantation of neural stem cells from brain of crossbred white rat in the neonatal period
Abstract: We have studied for isolating neural stem cells from brain tissue of neonatal crossbred white rat, culturing and transplanting into local cerebral ischemic reperfusion model rat. We performed digestion of brain tissue, primary culture and subculture of segregated cells, measured number of neurosphere and growth index and ratio of cell survival, and identified expression of nestine from cultured cells, investigated engraftment of NSCs into ill brain tissue after transplantation of …
Published in Research and Reviews: A Journal of Medicine · Vol. 12, Issue 2, 2022 · pp. 13–19 Read article
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A Review of Biotechnology Advancements as Key Drivers of Scientific Innovations
Abstract: The rising human challenges have necessitated the quest to find lasting solutions to improve living standards. Challenges in food security in terms of quality and quantity, medical and health issues, energy deficiency and limitations in areas of transportation, domestic and industrial needs, as well as environmental issues are some major areas of application of biotechnology to sustain human existence. This review examines major scientific innovations driven by biotechnology. Biotechnology applied …
Published in Research and Reviews : A Journal of Biotechnology · Vol. 15, Issue 3, 2025 · pp. 22–33 Read article
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Solanum viarum Dunal: Ethnomedicinal Importance, Phytochemical Profile and Pharmacological Potential — A Comprehensive Review
Abstract: Solanum viarum Dunal, commonly known as tropical soda apple or Sodom apple, is a medicinally important plant belonging to the family Solanaceae. The plant has been widely utilized in traditional medicine systems for the treatment of fever, cough, skin disorders, inflammation, gastrointestinal ailments, and microbial infections. The present review comprehensively summarizes the ethnomedicinal importance, phytochemical profile, pharmacological activities, and pharmaceutical significance of S. viarum. Literature reports indicate that the plant …
Published in Research & Reviews: A Journal of Pharmacognosy · Vol. 13, Issue 3, 2026 Read article
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Advancing Climate-Resilient Agriculture: Integrative Biotechnological Approaches for Sustainable Crop Production
Abstract: Climate change presents a major challenge to global agriculture by increasing the occurrence and severity of both non-living and living stress factors, such as drought, high temperatures, salt buildup, waterlogging, and new pests and diseases. These stressors interfere with the normal functioning of plants at the physical, chemical, and genetic levels, which in turn results in lower crop yields and poorer quality. While traditional methods of plant breeding played a …
Published in Research and Reviews : A Journal of Biotechnology · Vol. 16, Issue 2, 2026 Read article
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In vitro Cultivation of Trifolium Alexandrium L. as a Forage Crop for Sustainability of Pastoral Ecosystem
Abstract: Trifolium alexandrium L., commonly known as Berseem or clover, is an important leguminous winter fodder crop. In comparison to other legumes and grasses, clover provides forage with high nutritional value and quality. Hence, introduction of Berseem (clover) in existing pastoral ecosystem can provide value-added forage that can result in an increased level of animal production and simultaneously enhance stocking rates. Tissue culture is a technique through which we are able …
Published in Research and Reviews : A Journal of Biotechnology · Vol. 3, Issue 2, 2013 · pp. 6–11 Read article
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Micropropagation Protocol for Mass Multiplication of Stevia rebaudiana (Bert.) Bertoni: A Natural Sweetener
Abstract: AbstractStevia rebaudiana (Bert.) Bertoni is a small perennial herb of the family Asteraceae. It’s sweetening power ranges from 100–300 times more than sucrose. The sweet compounds found in Stevia leaves are diterpene glycosides (steviol glycosides), eight diterpene glycosides with sweetening properties have been identified in leaf tissue of stevia. It is reported to be hypoglycemic, hypotensive and effective in curing skin disorders, tooth decay etc. The market opportunity appears great …
Published in Research and Reviews : A Journal of Biotechnology · Vol. 5, Issue 1, 2015 · pp. 28–34 Read article
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Pattern of Cancer in Punjab (Cancer Capital of India)
Abstract: The problems of the nature and possible treatment of malignant disease are studied in many laboratories and results are published in different journals. It is difficult for any worker to keep in touch with all the published work. Excellent summaries of papers were published for many years in the Cancer Review, the publication of which was unfortunately suspended about ten years ago. Since then abstracts have been available in the …
Published in Research and Reviews : A Journal of Biotechnology · Vol. 11, Issue 2, 2021 · pp. 4–11 Read article
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Effect of IBA and BAP on in vitro Plant Regeneration of Mint
Abstract: AbstractAn experiment was conducted in the tissue culture laboratory of the department of horticulture, Bangabandhu Sheikh Mujibur Rahman Agricultural University (BSMRAU), Salna, Gazipur-1706, from March 2013 to February 2014 for in vitro regeneration of mint (Mentha sp) plantlets using different growth regulators in full strength MS media. Shoot tip was used as explant using three levels of BAP (1.0, 2.0 and 3.0 mg/l) and IBA (1.0, 2.0 and 3.0 mg/l) …
Published in Research and Reviews : Journal of Crop science and Technology · Vol. 4, Issue 3, 2015 · pp. 1–10 Read article