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11 articles for “RT-PCR”
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History, Treatment, Tool and Technique of Swine Flu
Abstract: The appearance of influenza A (H1N1) is more than 91 years has led to a universal pandemic. In the current scenario, swine influenza A (H1N1) (S-OIV) infections have been updated. On March 18, 2009, a reorganized flu virus was first reported from Mexico. This virus quickly circulates in neighboring United States and Canada and from there to the rest of the world. In India, H1N1 was first identified in April …
Published in Research and Reviews: A Journal of Microbiology and Virology · Vol. 10, Issue 3, 2020 · pp. 19–28 Read article
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Summer Outbreak of Influenza A (H1N1) at Tertiary Care Centre in Central India
Abstract: Background: Influenza is a highly contagious, acute, and febrile respiratory illness that occurs in epidemics and occasionally in pandemics. Post-pandemic resurgence with bi-modal peaks, a major peak in the rainy season, and a minor peak in the winter-spring season have been reported in India. However, a sudden increase in the number of cases of Influenza-like illness (ILI) was observed at our centre during the summer season. The study was conducted …
Published in Research and Reviews : A Journal of Immunology · Vol. 8, Issue 3, 2018 · pp. 24–31 Read article
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A Review on Current Scenario and Update on Vaccine for Covid-19
Abstract: Background: COVID-19 pandemic is caused by SARS-CoV-2 . The SARS-CoV-2 is a beta coronavirus belonging to the family of Coronaviridae. The outbreak of novel coronavirus had a major impact on health care professionals like physicians, pharmacist, microbiologist etc. Current scenario of detection of SARS-CoV-2(COVID-19) is made through a nasopharyngeal swab based on RT-PCR technique. Some novel recent studies suggest the evidence of the possible role of fluid which is present …
Published in Research and Reviews : A Journal of Immunology · Vol. 10, Issue 3, 2020 · pp. 1–7 Read article
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Molecular Surveillance of Spring Viraemia of Carp Virus (SVCV) and Koi Herpesvirus (KHV) Across Major Aquaculture Zones of Telangana, India
Abstract: Viral diseases represent a persistent threat to cyprinid aquaculture; however, region-specific surveillance data from tropical production systems remain limited. To address this gap, a comprehensive statewide active surveillance programme was undertaken across Telangana, India, from 2019 to 2022 to investigate the occurrence of two priority transboundary pathogens, Spring Viraemia of Carp Virus (SVCV) and Koi Herpesvirus (KHV). A total of 414 fish specimens were systematically collected from five surveillance sectors …
Published in International Journal of Virus Studies · Vol. 3, Issue 2, 2026 Read article
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A Comprehensive Review on COVID-19 Evolution and Development
Abstract: The emerging pandemic causing flu-like symptoms in patients is caused by the novel Coronavirus that was previously called 2019-nCoV. The mutated gene of SARS-CoV was transformed to SARS-CoV-2 in animals and further transmitted to humans in the month of December 2019 in Wuhan city of China. Eventually, the novel coronavirus spread all around the globe as a highly contagious disease. COVID-19 (the illness caused by coronavirus) has affected 213 countries …
Published in Research and Reviews : A Journal of Life Sciences Read article
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COVID-19 Resurgence in 2025: India’s Continuing Public Health Challenge
Abstract: The year 2025 has witnessed the resurgence of COVID-19, reigniting concerns at both global and national levels regarding the persistent threat posed by SARS-CoV-2. In early 2025, India witnessed a moderate resurgence of COVID‑19, characterized by the emergence of Omicron sub-variants and a rise in mild-to-moderate infections. As of mid-June, active COVID‑19 cases in India totaled approximately 6,000–7,400, with hotspots in Kerala (~2,100 cases), Delhi (~600), Maharashtra (~500), and several …
Published in International Journal of Tropical Medicines · Vol. 2, Issue 2, 2025 · pp. 16–23 Read article
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Isolation and Characterization of “Heavy Chain Antibody” Variable Domain Gene of Indian Desert Camel (Camelus dromedarius, L.)
Abstract: Camelids have “heavy chain antibodies” (HCAbs) of IgG isotype (IgG2 and IgG3 subtypes) and lack light chains and heavy chain constant domain 1 (CH1) in their structure. The molecular biological features of VHH (i.e., antigen binding variable domain of HCAbs) gene isolated from Indian desert camel were examined as a prelude to construct VHHs-phage display library. VHH cDNA was synthesized by reverse transcription-polymerase chain reaction (RT-PCR) from B lymphocyte mRNA, …
Published in Research and Reviews : A Journal of Immunology · Vol. 3, Issue 1, 2013 · pp. 22–31 Read article
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Covid 19: Virology, Pathogenesis, Epidemiology and Control
Abstract: AbstractThe pneumonia caused by novel coronavirus (SARS-CoV-2) in Wuhan, China in December2019 is a highly infectious disease. Coronavirus disease spreads primarily through contact with an infected person when they cough or sneeze. It also spreads when a person touches a surface or object that has the virus on it, then touches their eyes, nose, or mouth. The World Health Organization (WHO) has declared the ongoing outbreak as a global public …
Published in Research and Reviews : A Journal of Immunology · Vol. 10, Issue 2, 2020 · pp. 6–17 Read article
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COVID-19: Impact on Human Health
Abstract: Researchers are on the cutting edge of devising the best strategy to combat the SARS-CoV-2 pandemic by studying and exploring the pathogenesis, prevention, and treatment as the world continues to be plagued by an increasing number of confirmed COVID-19 cases. The primary method for detecting the virus is via antigen testing and more accurate molecular tests such as rt PCR using patient samples. The most prevalent treatments for COVID-19 infection …
Published in Research and Reviews : A Journal of Immunology · Vol. 12, Issue 1, 2022 · pp. 1–8 Read article
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A Review- Recent Outbreak of Zika Viruses in India, its Vectorology and Virology
Abstract: Zika virus is an arbovirus (arthropod-borne virus) which belongs to the family Flaviviridae that was first isolated in 1947 from blood of a febrile rhesus macaque monkey during a yellow fever study in the Zika forest of Uganda and later identified in Aedes africanus mosquito from the same forest. But in 2007, the first major outbreak of Zika virus infection occurred in Yap Island (Federated States of Micronesia), where, it …
Published in Research and Reviews: A Journal of Microbiology and Virology · Vol. 9, Issue 2, 2019 · pp. 25–33 Read article
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Molecular Characterization of Respiratory Viral Infections and Co-Infections in Pediatric Patients Using FilmArray™ Multiplex PCR in a Tertiary-Care Hospital, South India
Abstract: Respiratory tract infections (RTIs) are a major cause of pediatric morbidity worldwide, with viruses, such as respiratory syncytial virus, rhinovirus/enterovirus, influenza virus, adenovirus, and parainfluenza virus, contribute significantly to hospitalizations. Conventional diagnostic methods often require prolonged processing times, which can delay clinical decision-making. Rapid molecular platforms, such as the FilmArray™ Panels, offer timely and comprehensive pathogen identification. This study aimed to evaluate the prevalence, seasonal trends, and co-infection patterns of …
Published in Research and Reviews: A Journal of Microbiology and Virology · Vol. 16, Issue 2, 2026 · pp. 57–66 Read article