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14 articles for “R-Plasmid”
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PCR identification of blaTEM gene mediated for ESBL drug resistance in Salmonella typhi
Abstract: Objective: The aim of the present study to identify the presence of R-plasmid and detect the blaTEM gene encodes for ampicillin resistance.Materials and Methods: Salmonella typhi organisms isolated from widal positive blood samples and screened by standard screening methods. Antimicrobial susceptibility test by Kirby-Bauer disc diffusion method and MIC by agar dilution method for Ampicillin performed as per Clinical Laboratory Standard Institute (CLSI) guidelines. ESBL detection by phenotypic confirmatory test, …
Published in Research and Reviews: A Journal of Microbiology and Virology · Vol. 6, Issue 2, 2016 · pp. 1–6 Read article
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Recombinant Protein Manufacturing Using Actinomycetes as Host Cells
Abstract: Actinobacteria are highly sought-after for use as cell factories or bioreactors in the pharmaceutical, agricultural, industrial, and environmental sectors. The commercial production of these proteins as recombinants and the biochemical and structural characterization of key proteins have been made possible by the genome sequencing of numerous species of actinomycetes. In this regard, better expression vectors that may be used with actinomycetes are required. As discussed in this article, recent developments …
Published in Research & Reviews: A Journal of Bioinformatics · pp. 23–27 Read article
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Hairy Root Culture for Mass Production of Medicinal Important Compound Neoandrographolide from Andrographis lobelioides Nees
Abstract: AbstractThe present study was focused to develop hairy root culture of Andrographis lobelioides for the production of Neoandrographolide using Agrobacterium rhizogenes (8196), strains for leaf and root explants. The results indicated that the leaf and root explants induced hairy roots formation and enhanced secondary metabolites Neoandrographolide and related compounds. The medium was supplemented with half strength liquid MS medium with 2.0 μM/l Indole butyric acid (IBA). The highest percentage of …
Published in Research and Reviews : A Journal of Biotechnology · Vol. 8, Issue 2, 2018 · pp. 14–19 Read article
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Antibiotic Resistance in Microbes: History, Mechanisms, Therapeutic Approaches, and Strategies for Mitigation
Abstract: The global emergence of clinically significant antibiotic resistance is increasingly linked to the environment. Antimicrobials, metals, and biocides are just a few of the substances that are released into the environment through paths that are regulated by environmental regulators. Consequently, environmental regulators ought to be heavily involved in developing national and international antimicrobial resistance (AMR) measures. Antimicrobial compounds from pharmaceutical waste, agricultural runoff, and wastewater discharge contribute to the spread …
Published in International Journal of Antibiotics · Vol. 2, Issue 2, 2025 · pp. 1–11 Read article
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TOXICOLOGY: Present-Day Difficulties for Investigating Safety Science and Target Safety Evaluation of Major Human Hepatocytes
Abstract: Preclinical toxicology has developed into a more thorough understanding of toxicity pathways from its descriptive beginnings. Technological developments have made it possible to forecast safety occurrences, mitigate adverse effects, and create safety biomarkers. Because they are able to self-renew and specialize into different types of human body cells, stem cells are currently being employed for in vitro toxicological applications. However, as compared to primary human hepatocytes, stem cell-derived HLCs perform …
Published in Research and Reviews: A Journal of Toxicology · Vol. 14, Issue 3, 2024 · pp. 31–40 Read article
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Harnessing Bacteria for Next-Generation Data Storage Technologies – A Review
Abstract: The exponential increase in global digital information has created a pressing need for storage technologies that are more durable, compact, and sustainable than conventional electronic media. While hard drives, solid-state drives, and cloud-based systems have transformed information management, they face severe limitations related to storage density, energy consumption, maintenance costs, and long-term preservation. Researchers have, therefore, begun exploring biological systems as alternative information storage platforms. Among these, bacteria have emerged …
Published in International Journal of Molecular Biotechnological Research · Vol. 4, Issue 2, 2026 · pp. 1–9 Read article
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A Method of Universal Primer Design for The Detection of Diverged CTX-M Beta-Lactamases in Multi-Drug Resistant Superbugs
Abstract: Complexity of many diverged β-lactamases in common multi-drug resistant bacteria has reached very high level. We found >40% of common bacteria in Kolkata water bodies were ampicillin resistant and reported clinical isolates were >95% ampicillin resistant worldwide. More than twenty unique sequence classes of β-lactamases were known to cause MDR inactivating penicillin, cephalosporin and carbapenem drugs. Further, there are other diverged MDR genes like drug efflux genes (acrAB, mexAB-EF, tetA), …
Published in Research and Reviews : A Journal of Biotechnology · Vol. 9, Issue 2, 2019 · pp. 1–10 Read article
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Construction and Development of pSB111 Bar Plasmid Vector for Activation Tagging
Abstract: To get desired traits and to know the precise expression patterns of genes in the plant, Agrobacterium tumefaciens has been widely employed in the generation of transgenic plants using plasmid vectors. Many methods are come forward to establish the gene expression of unknown genes but in these techniques gene alteration isthe main drawback orsometimesit islethal to organisms also, to overcome these problems activator tag method developed to know the specific …
Published in Research & Reviews : Journal of Botany · pp. 1–6 Read article
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Extended-spectrum beta-lactamases (ESBLs) and Metallo-beta-lactamases (MBLs): A Review
Abstract: Gram-negative bacteria produce extended-spectrum beta-lactamases (ESBLs) and metallo-beta-lactamases (MBLs), enzymes that play a crucial role in antibiotic resistance. These enzymes enable the bacteria to withstand a wide array of beta-lactam antibiotics, including penicillins, cephalosporins, and carbapenems. The production of these enzymes, particularly by organisms, such as Escherichia coli and Klebsiella pneumoniae, complicates treatment options for severe infections, leading to increased morbidity and mortality in clinical settings. ESBLs are primarily responsible …
Published in Research and Reviews: A Journal of Microbiology and Virology · Vol. 15, Issue 2, 2025 · pp. 8–19 Read article
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Carbon Nano Tubes: New Techniques for Cancer Treatment
Abstract: Carbon Nano tubes (CNTs) have been recently introduced as a novel carrier system in the biomedical field and produce small and large therapeutic molecules. CNTs have attracted increasing attention for their versatile applications. Based on layers of CNTs, CNTs are generally divided into two categories: single-walled carbon Nano tubes (SWCNTs), multi-walled carbon Nano tubes (MWCNTs). It shows the unique properties and cylindrical shape structure. It shows a greater advantage in …
Published in Trends in Drug Delivery · Vol. 5, Issue 1, 2018 · pp. 28–39 Read article
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CRISPR-Cas System: Next Generation Genome Editing Tool
Abstract: CRISPR-Cas system provides sequence specific adaptive immunity against phages and mobile genetic elements. Resistance is acquired following phage infection or plasmid uptake when short specific sequence is added into CRSIPR array. This complex guides cRNA to target complementary DNA fragments and target its degradation. CRISPR array is then transcribed and processed into cRNA which forms complex with cas protein. The ability of CRISPR-Cas system to induce site specific DNA breaks …
Published in Research and Reviews : A Journal of Biotechnology · Vol. 6, Issue 2, 2016 · pp. 10–16 Read article
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Review: Virulence factors, resistance genes and pathogenicity of Moraxella catarrhalis
Abstract: An estimated 2-4 million cases of chronic obstructive pulmonary disease(COPD) in adults are exacerbated by Moraxella catarrhalis annually in the US, making it a pathogen of increasing significance for respiratory tract infections. It often colonizes the nasopharynx without causing any symptoms and an opportunistic bacterial infection of the respiratory mucosa that is exclusive to humans. In our study, we reviewed references about the important virulence factors that M. catarrhalis possess …
Published in Recent Trends in Infectious Diseases · Vol. 2, Issue 1, 2025 · pp. 33–40 Read article
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Molecular Cloning, Expression, Purification, and Characterization of Methionine Aminopeptidase
Abstract: MAP enzyme can efficiently remove N-terminal methionine from recombinant proteins. In the present study, MAP gene from genomic DNA of Escherichia coli was amplified, cloned into pET 11b vector and transformed into E.coli DH5a cloning strain to confirm the insertion of MAP gene, then plasmid was transformed into E. coli BL21 (DE3) expression strain. The protein was eluted with 25 mM NaCl and showed a purity of ~ 95% which …
Published in Research and Reviews: A Journal of Microbiology and Virology · Vol. 2, Issue 2, 2012 · pp. 12–24 Read article
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Transgenic Expression of Stress Tolerance Gene in Sugarcane (Saccharum officinarum L.)
Abstract: AbstractAgrobacterium tumefaciens LBA4404 mediated transformation of sugarcane variety 31 with two plasmids of pB1121 constructs, one containing PsCBL gene and the other containing PsCIPK gene was studied for stress tolerance capability induction. Calli of the variety Isd 31 were infected with both plasmid containing strains using three different infection times of 60, 90 and 120 min in acetosyringone infection medium. In GUS (β-glucuronidase) assay, randomly selected calli from 120 min …
Published in Research & Reviews : Journal of Agricultural Science and Technology · Vol. 6, Issue 2, 2017 · pp. 32–39 Read article