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5 articles for “IPTG”
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Molecular Cloning, Expression, Purification, and Characterization of Methionine Aminopeptidase
Abstract: MAP enzyme can efficiently remove N-terminal methionine from recombinant proteins. In the present study, MAP gene from genomic DNA of Escherichia coli was amplified, cloned into pET 11b vector and transformed into E.coli DH5a cloning strain to confirm the insertion of MAP gene, then plasmid was transformed into E. coli BL21 (DE3) expression strain. The protein was eluted with 25 mM NaCl and showed a purity of ~ 95% which …
Published in Research and Reviews: A Journal of Microbiology and Virology · Vol. 2, Issue 2, 2012 · pp. 12–24 Read article
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Preliminary In Silico Analysis of Choline Monooxygenase and Betaine Aldehyde Dehydrogenase from Suaeda monoica Forssk. and its Expression in E. coli.
Abstract: AbstractIn a tropical country like India, the yield of crop plants is majorly affected by soil salinity and drought. One of the strategies to address this problem is by introducing genes responsible for salinity/drought tolerance in crop plants. Such genes could be identified and isolated from mangrove plants, as they are known to have evolved certain physiological mechanisms to augment saline environments. One such mechanism is the accumulation of Glycine …
Published in Research and Reviews : Journal of Computational Biology · Vol. 9, Issue 1, 2020 · pp. 10–39 Read article
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Application of pET28a Vector for the Over Expression of Recombinant Human Tim23 Protein in E. coli and its Validation by Immunoblotting
Abstract: This study aimed to produce recombinant human Tim23 protein by molecular cloning, expression, and purification. Amplification of human TIM23 was carried out by polymerase chain reaction (PCR) and cloned into DH5α cells. The pET28a-human TIM23 was transformed into BL21DE3 and expressed the gene by IPTG induction. Recombinant pure human Tim23 antigen was prepared, and injected into rabbits and polyclonal antibodies were raised, which was confirmed by western blotting. The transformed …
Published in Research and Reviews : A Journal of Life Sciences · Vol. 14, Issue 1, 2024 · pp. 32–44 Read article
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Cloning and Expression of LPS-Binding Single Domain Antibody Clones Selected from Phage Display Library of Indian Desert Camel
Abstract: Despite the absence of light chains in their structure, camelid and shark “heavy chain antibodies” (HCAbs) variable domains (designated as VHH) are able to make functional paratopes. The authors have selected lipopolysaccharide (LPS)-binder “single-domain antibodies” (dAb) clones from the previously constructed phage display library of (LPS)-immunized Indian desert camel. This study was undertaken to make available large amounts of some LPS-binder dAb clones by using a suitable expression vector-host system …
Published in Research and Reviews : A Journal of Immunology · Vol. 4, Issue 1, 2014 · pp. 1–7 Read article
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A Single-domain Antibody Cross-reacts with Lipopolysaccharide from Diverse Gram-negative Bacteria
Abstract: Gram-negative bacterial sepsis and endotoxemia are the leading causes of morbidity and mortality in animals and humans worldwide, and attempts have continued to design rational therapies. Endotoxin/lipopolysaccharide (LPS)-neutralizing polyclonal and monoclonal antibodies have been produced, but are not free from problems. Single-domain antibodies (dAbs), constructed from the camelid and the shark ‘heavy chain antibodies’ by phage display technology possess favorable features for in vivo applications. The objective of this study …
Published in Research and Reviews : A Journal of Immunology · Vol. 6, Issue 2, 2016 · pp. 29–37 Read article