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8 articles for “Agar- agar gel”
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Spectroscopic and Thermal Investigations of Barium Tartrate Crystals Grown in Agar-Agar Gel
Abstract: Barium tartrate chargers were synthesized using the gel system with a single prolixity fashion at room temperature in a 1 w/ v agar- agar gel as the growth medium. The reactants, 1M BaCl₂ (dihydrate) and 1M disodium tartrate, were introduced into the gel and allowed to interact for a period of 25 days. Completely developed white, candescent, spherulitic-structured chargers of barium tartrate were gathered later. Infrared spectroscopy( FTIR) revealed characteristic …
Published in International Journal of Universe · Vol. 1, Issue 1, 2025 Read article
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Application of pET28a Vector for the Over Expression of Recombinant Human Tim23 Protein in E. coli and its Validation by Immunoblotting
Abstract: This study aimed to produce recombinant human Tim23 protein by molecular cloning, expression, and purification. Amplification of human TIM23 was carried out by polymerase chain reaction (PCR) and cloned into DH5α cells. The pET28a-human TIM23 was transformed into BL21DE3 and expressed the gene by IPTG induction. Recombinant pure human Tim23 antigen was prepared, and injected into rabbits and polyclonal antibodies were raised, which was confirmed by western blotting. The transformed …
Published in Research and Reviews : A Journal of Life Sciences · Vol. 14, Issue 1, 2024 · pp. 32–44 Read article
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Cadmium Accumulation in Different Tissues and DNA Content Alteration in Cat Fish (Clarias batrachus) Exposed to Lethal Concentration of Cadmium
Abstract: In the present study, the acute toxicity experiment was conducted to evaluate LC50 of Cadmium chloride (CdCl2) on Clarias batrachus which was found to be 450 ppm for 96 h. Clarias batrachus (average length of 8.5 ± 0.4 cm and the average weight of 13.5 ± 0.393 g) was exposed to sub-lethal concentration (140 ppm) and lethal (450 ppm) concentrations of Cd for 14 days. The Cd accumulation in tissues …
Published in Research and Reviews: A Journal of Toxicology · Vol. 10, Issue 3, 2020 · pp. 1–8 Read article
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A Duplex PCR for Simultaneous Detection of Brucella and Bovine Herpesvirus – Genome in Clinical Samples
Abstract: Normal 0 false false false EN-US X-NONE X-NONE MicrosoftInternetExplorer4 A disease-free germplasm is a prerequisite for getting healthy progeny. Many diseases are transmitted through semen either by natural service or artificial insemination causing reproduction failure and economic losses. The intermittent excretion of Bovine Herpesvirus-1 (BoHV-1) and Brucella in semen and their transmission through artificial insemination has been recorded. PCR has become an invaluable tool because of speed and simplicity with …
Published in Research and Reviews: A Journal of Microbiology and Virology · Vol. 1, Issue 2, 2011 · pp. 1–7 Read article
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Existence of Integron I and II Genes from Clinical Isolate Staphylococcus aureus and Their Role in Drug Resistance
Abstract: An integron is a gene capture and dissemination system. They have been identified on both genomic and mobile genetic elements which often contain one or more genes that encode antibiotic resistance. They are commonly associated with bacterial genera in the family of Enterobacteriaceae. Currently, there have been recent reports showing the presence of integrons in Staphylococcus species. The aim of the present study was to assess the existanxe of Class …
Published in Research and Reviews: A Journal of Microbiology and Virology · Vol. 4, Issue 3, 2014 · pp. 18–24 Read article
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Semi-quantitative Event Specific Detection of Bt Maize (Event MON 810) using Real-Time PCR with SYBR Green Ι Chemistry
Abstract: The objective is this work is to do semi-quantitative analysis for Bt maize (event MON 810) using conventional Polymerase Chain Reaction (PCR) and Real Time PCR (SYBR green chemistry) from the fresh maize samples chosen arbitrarily from various locations. The protocol for extraction of good quality genomic Deoxyribonucleic acid (DNA) was standardized without the use of Liquid nitrogen. The DNA used for further studies were obtained by using standardized protocol. …
Published in Research and Reviews : A Journal of Biotechnology · Vol. 2, Issue 3, 2012 · pp. 25–34 Read article
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Review on Advanced Molecular Diagnostic Tools and Their Applications
Abstract: Although the diagnosis of diseases is still primarily based on conventional methods (staining, culture, biochemical analysis), a series of advanced molecular diagnostic tools are increasingly introduced and incorporated in the workflow of clinical laboratories worldwide. These tools, such as polymerase chain reaction, northern blotting, restriction length polymorphism, flow cytometry, southern blotting, fluorescent in situ hybridization, agarose gel electrophoresis and pyrosequencing that are based on either the properties of nucleic acids …
Published in Research and Reviews : A Journal of Life Sciences · Vol. 3, Issue 3, 2013 · pp. 21–33 Read article
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Formulation and Evaluation of Topical Antiacne Gel from Coriander and Garlic Extract
Abstract: Objective: To develop and evaluate a topical anti-acne gel formulated using aqueous extracts of coriander (Coriandrum sativum) and garlic (Allium sativum). The study aims to assess the antibacterial efficacy of the gel against acne-causing bacteria, specifically Propionibacterium acnes. Methods: 1.Extraction: Coriander Extract: Aqueous extraction was performed by soaking coriander seeds in distilled water, followed by filtration. Garlic Extract: Non-chemical extraction involved mixing crushed garlic with honey in a 1:1 ratio …
Published in Research & Reviews: A Journal of Pharmacognosy · Vol. 11, Issue 3, 2024 · pp. 10–15 Read article