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649 articles for “isolate”
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Photovoltaic Integrated Electric Vehicles Charging stations for isolated locations
Abstract: Future mobility plans of global countries focus on replacement of IC engines operated mechanism by electrically powered systems. It aims to reduce carbon footprints, improve fuel efficiency and make vehicle systems smarter. A typical electric vehicle is an integration of battery banks, charge management systems, electric motors, power electronic converters, and control mechanisms. The battery bank maintains the supply of energy for the operation of the motor which in turn …
Published in Trends in Electrical Engineering · Vol. 13, Issue 1, 2023 · pp. 1–11 Read article
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The study on the isolation, culture, differentiation and transplantation of neural stem cells from brain of crossbred white rat in the neonatal period
Abstract: We have studied for isolating neural stem cells from brain tissue of neonatal crossbred white rat, culturing and transplanting into local cerebral ischemic reperfusion model rat. We performed digestion of brain tissue, primary culture and subculture of segregated cells, measured number of neurosphere and growth index and ratio of cell survival, and identified expression of nestine from cultured cells, investigated engraftment of NSCs into ill brain tissue after transplantation of …
Published in Research and Reviews: A Journal of Medicine · Vol. 12, Issue 2, 2022 · pp. 13–19 Read article
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Prevalence and Antibiogram Profile of Uropathogens Isolated from Symptomatic and Asymptomatic Female Patients with Urinary Tract Infections and its Associated Risk Factors: Focus on Cephalosporin
Abstract: Urinary tract infections (UTIs) are common among women. Here, the present study probes the prevalence of urinary tract infection (UTI), assess the adequacy of empirical therapy, susceptibility of antibiotics and resistance pattern of bacteria responsible for UTI and its associated risk factors. A total of 1309 urine samples were collected from UTI suspected female patients of tertiary care hospitals between the ages of 18-49. Moreover, a total of 207 urine …
Published in Research and Reviews : A Journal of Medical Science and Technology · Vol. 7, Issue 1, 2018 · pp. 32–41 Read article
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Isolation and Characterization of Rhamnolipid from Pseudomonas aeruginosa: Assessment of Biofilm Disruption Potential
Abstract: Biosurfactants are microbial metabolites bearing typical amphiphilic structure of a surfactant. This study is aimed at isolation and characterization of Rhamnolipid (RL) biosurfactant from Pseudomonas aeruginosa MTCC 2488. Characterization of biosurfactant was assessed by oil displacement test, foaming test, thin layer chromatography (TLC) and antimicrobial activity for the same was evaluated against planktonic/sessile microbes including Staphylococcus aureus, Bacillus subtilis and Candida albicans. Keywords: Biosurfactant, rhamnolipid, biofilm, Pseudomonas aeruginosaCite this ArticleKaur …
Published in Research and Reviews: A Journal of Microbiology and Virology · Vol. 8, Issue 2, 2018 · pp. 26–33 Read article
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Characterization of Laccase Enzyme from Dye Degrading Bacterial Strains Isolated from Natural Sources
Abstract: The continuous growth in globalization and industrialization has increased the environmental pollution and one such major contaminant is various types of synthetic dyes. Microbial decolorization and degradation of these dyes through secretion of various extracellular enzymes is gaining importance owing to their eco-friendly nature. One such enzyme is laccase, a multicopper oxidase enzyme which has the ability to oxidize both phenolic and non-phenolic compounds through reduction of molecular oxygen to …
Published in Research and Reviews: A Journal of Microbiology and Virology · Vol. 9, Issue 1, 2019 · pp. 65–76 Read article
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Evaluation of Cholesterol Reduction in Probiotic Strain Lactobacillus brevis isolated from Human Fecal Sample by Using Surface Response Methodology
Abstract: Lactobacillus brevis, isolated from human feces was assessed for its in vitro cholesterol reduction ability. The effects of bile salts, inoculum size and contact time in simulated intestinal juice were studied. Central composite design model was utilised to determine the effects of interactions of significant variables on cholesterol reduction. By developing a linear regression model, as a function of growth associated variables in vitro cholesterol reduction was assessed. The results …
Published in Research and Reviews: A Journal of Microbiology and Virology · Vol. 9, Issue 1, 2019 · pp. 40–49 Read article
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Isolation and Characterization of Protease Producing Organism Bacillus Species from Slaughter House Waste
Abstract: The purpose of this investigation was to isolate protease producing bacteria from slaughter house waste collected in local area of Kaliakkavilai. The Bacillus sp. was identified by several biochemical and physiological tests. The effect of different production parameters such as pH, temperature, metal ions concentration and substrate concentration for protease production by the isolated bacterial strain were studied. The enzyme production was assayed in submerged fermentation with optimized parameters to …
Published in Research and Reviews: A Journal of Microbiology and Virology · Vol. 3, Issue 3, 2013 · pp. 8–14 Read article
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Biofilm Forming and Antimicrobial Susceptibility of Clinical Isolates of Staphylococcus Species
Abstract: Staphylococcus species are the most frequent causes of infection and infections on indwelling medical devices, which characteristically involve biofilms. This study observes the biofilm forming abilities and to analyze antimicrobial susceptibility pattern of clinical isolates of Staphylococcus species were found to be biofilm producers by microtitter plate assay method. And to evaluate the antibiofilm property of Moringa oleifera an Indian medicinal plant, known to possess antimicrobial activity. The results demonstrate …
Published in Research and Reviews: A Journal of Microbiology and Virology · Vol. 5, Issue 2, 2015 · pp. 1–4 Read article
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Isolation and Characterization of β -D-Glucan from Yeast and its In Vitro Biological Study
Abstract: In many microorganisms, fungi and algae, β-D-glucan can be found as cell wall polysaccharide. Various publications describe the strong positive influence of these glucans on the immune system comprising antibacterial, woundhealing and antitumor activities. Recently, it was found that β-D-glucan from the yeast Saccharomyces cervisiae also exhibit antioxidative capabilities. In the present study, the β-D-glucan was isolated from the cell wall of yeast and it is characterized by chromatographic, spectroscopic …
Published in Research and Reviews : A Journal of Biotechnology · Vol. 2, Issue 1, 2012 · pp. 16–23 Read article
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In silico PASS-Assisted Biological Activity Predictions of a Phytoconstituent Astilbin isolated from Thelypteroid fern Sphaerostephanos arbuscula (Willd.) Holttum
Abstract: Pteridophytes are the most important land plants with enormous medicinal properties. The medicinal properties of this group of plants mainly depend upon the numerous phytoconstituents present inside the plants. In the present study, the biological activity spectra of a phytoconstituent Astilbin isolated from Thelypteroid fern Sphaerostephanos arbuscula was predicted using computer-aided program—Prediction of Activity Spectra for Substances (PASS). The PASS predicted activity of Astilbin exhibited its various probable activities (Pa). …
Published in Research and Reviews : A Journal of Biotechnology · Vol. 6, Issue 2, 2016 · pp. 24–29 Read article
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Biotechnological Prospective of a Group of Beneficial Extremophiles Isolated from Different Sources
Abstract: Extremophiles thrive in terrestrial hot springs, hydrothermal vents, glacial ices, saline lakes, solar saltern and acidic and alkaline niches; moreover a group of the extremophiles may grow in household hazardous waste, solvents containing cleaning and polishing chemicals, heavy metals contaminated sites, or in several other habitats such as aphotic, anoxic, athalassohaline and thalassohaline habitats that were previously considered inhospitable for life. Beside aforementioned extreme niches, extremophiles also dwell in poultry …
Published in Research and Reviews : A Journal of Biotechnology · Vol. 10, Issue 2, 2020 · pp. 10–13 Read article
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Antimicrobial Activity of Actinomycetes Isolated from Garden Soil and Role of Various Antibiotics in Promoting Its Growth
Abstract: AbstractActinomycetes are a group of Gram-positive, sporulating, bacteria which exhibit mycelia branching during growth. These are common inhabitants of soil. They provide many important bioactive compounds which are of high commercial value. However, antibiotics are predominately produced. In the present study, actinomycetes was isolated from garden soil and its growth was tested in different media such as Kustners media, actinomycetes isolation agar, yeast extract agar and Kenknights agar. The culture …
Published in Research & Reviews : Journal of Agricultural Science and Technology · Vol. 2, Issue 2, 2013 · pp. 1–8 Read article
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Effect of Prebiotics on Growth of the Selected Lactobacilli Culture Isolated from Dairy Products
Abstract: Prebiotics beneficially affects the host by selectively stimulating the growth or activity of one or limited number of bacteria such as bifidobacteria, lactobacilli in the colon that can improve the host health. Inulin and gum acacia are the most commonly available prebiotics. The ability of lactobacilli cultures (i.e. Lactobacillus acidophilus, Lactobacillus plantarum and Lactobacillus casei) to utilize inulin, gum acacia, honey and fructo-oligosaccharide @ 2% for different time intervals (6, …
Published in Research and Reviews : Journal of Dairy Science and Technology · Vol. 2, Issue 2, 2013 · pp. 1–8 Read article
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Isolation of Lactobacilli sp. from Various Nondiary Substrates—A Review
Abstract: Lactic acid bacteria (LAB) have also been delineated as bioprotective agents. Efficient pathogens’ inhibition has been derived by LAB. LAB competes with the microbes by modifying the microenvironment with their metabolic end products. The habitat of Lactobacilli is not only confined to diary but it has spread and adapted habitat to nondiary substrates too. This paper garners information about the various nonsubstrate habitats in which Lactobacilli can be isolated. Keywords: …
Published in Research and Reviews : Journal of Dairy Science and Technology · Vol. 7, Issue 2, 2018 · pp. 21–24 Read article
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Isolation and Identification of Lactobacillus from Raw Buffalo Milk from the Local Region of Agra
Abstract: The term ‘Probiotic’, literally means, ‘for life’ which describes “substances secreted by one microorganism stimulating the growth of another”. These are viable microorganisms which contribute to intestinal microbial balance and help to improve the gut health in humans and other animals. Probiotics have been used in animal feed industry as a food supplements. They are also used to manufacture the fermented dairy products widely. The most common among them are …
Published in Research and Reviews : Journal of Dairy Science and Technology · Vol. 9, Issue 2-3, 2020 · pp. 15–17 Read article
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Optimization of Red Pigment Yield from Monscus purpureus Solid State Fermentation (Isolation and Characterization of Monscus purpureus and Optimization of its Red Pigment Production)
Abstract: Natural pigments production is one of the important approaches recently for being natural and having therapeutic properties. Monscus purpureus was isolated from imported red yeast rice, characterized and propagated on local rice as solid state culture. Morphological characteristics which include colonies, ascosporous and conidial stage were used to identify the fungi besides its red pigment production. Red pigment was produced using 10% of seed culture of Monescus purpureus, incubated at …
Published in Research & Reviews : Journal of Food Science & Technology · Vol. 4, Issue 2, 2015 · pp. 21–29 Read article
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Isolation and Identification of Mycoflora from Floor Dust Via Non-culturable and Culturable Method: A Case Study of Indoor Atmospheric Quality (IAQ)
Abstract: The concentration of fungal genera in the floor dust was studied by culturable and non-culturable methods. A total of eight fungal genera were isolated and identified out of which two were characterized by non-culturable method, one with culturable method and other five were common for both the methods. The predominant genera were Aspergillus, Cladosporium Penicillium, Fusarium and Curvularia. From the comparative data obtained from culturable and non-culturable fungal concentration it …
Published in Research and Reviews : A Journal of Life Sciences · Vol. 9, Issue 1, 2019 · pp. 26–31 Read article
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Amplification of 16s rRNA Gene of Brucella melitensis Causing Brucellosis Isolated from the Aborted Fetes of Goat
Abstract: ABSTRACTBrucella melitensis is zoonosis caused by facultative intracellular bacteria of genus Brucella, which are widely distributed in both human and animals, especially in developing world. The diagnosis of goat brucellosis requires isolation of the bacteria from stomach contain aborted fetes of goat. However cultural sampling sensitivity often low, depending on the disease stage, Brucella species, culture medium, quantity of circulating bacteria and blood culture technique employed. The development of PCR …
Published in Research and Reviews : Journal of Veterinary Science and Technology · Vol. 9, Issue 1, 2020 · pp. 8–12 Read article
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Antibacterial Activity of Leaf and Flower Extract of Lablab purpureus against Clinical Isolates of Staphylococcus aureus
Abstract: The present study was undertaken to determine inhibitory activity of leaf and flower extracts of Lablab purpureus against clinical isolates of Staphylococcus aureus. Agar well diffusion assay was performed to determine antibacterial activity. Among extracts, the flower extract has shown marked inhibition of S. aureus isolates. It can be concluded that the leaf and flower extracts possess inhibitory components. The bioactive components present in the plant are to be separated …
Published in Research & Reviews: A Journal of Drug Design & Discovery · Vol. 1, Issue 2, 2014 · pp. 5–7 Read article
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Assessment of Antibacterial Potentialities of Diacetaldehyde Terpenoid Isolated from Curcuma Caesia Roxb. Against a Panel of Pathogenic Bacteria
Abstract: Assessment of antibacterial potentialities of a diacetaldehyde terpenoid was performed in this paper against a panel of pathogenic bacterial strains. This terpenoid was isolated from Curcuma caesia Roxb. and found to have several kinds of biological activities like antibacterial, antifungal and antitumour. In this experiment it was found that the terpenoid can inhibit the growth of bacterium at 150 to 200 μg/ml of concentrations. Hence, this terpenoid can be used …
Published in Research and Reviews: A Journal of Pharmaceutical Science · Vol. 11, Issue 1, 2020 · pp. 13–16 Read article