Isolation and Identification of Mycoflora from Floor Dust Via Non-culturable and Culturable Method: A Case Study of Indoor Atmospheric Quality (IAQ)

Pintu Karmakar; Kripamoy Chakraborty; Panna Das; Ajay Krishna Saha

doi:10.37591/rrjols.v9i1.1383

Abstract

The concentration of fungal genera in the floor dust was studied by culturable and non-culturable methods. A total of eight fungal genera were isolated and identified out of which two were characterized by non-culturable method, one with culturable method and other five were common for both the methods. The predominant genera were Aspergillus, Cladosporium Penicillium, Fusarium and Curvularia. From the comparative data obtained from culturable and non-culturable fungal concentration it was evident that the non-culturable fungal spore count was higher than the fungal CFU/g of dust sample in total counts. In respect to the number of fungal genera it was more in non-culturable method than the culturable method. Torula sp., Nigrospora sp., and fungal fragments were not observed in culturable method. However, this is the first report of identification of fungal fungal spores from dust samples by following non-culturable method. Moreover, this non-culturable method can be useful for quantification of the fungi from dust as these fungal genera fail to sporulate in synthetic media. Both the methods are complementary to each other for tangible quantification of the total fungi in a microhabitat.Keywords: Culturable and non-culturable method, predominant, first report, complementary, microhabitatCite this ArticlePintu Karmakar, Kripamoy Chakraborty, Panna Das, Ajay Krishna Saha. Isolation and Identification of Mycoflora from Floor Dust Via Non-culturable and Culturable Method: A Case Study of Indoor Atmospheric Quality (IAQ). Research & Reviews: A Journal of Life Sciences. 2019; 9(1): 26–31p.