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32 articles for “mutant.”
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Assessment of Animal Trials Conducted on Protectivity of Potential Sub-unit and Recombinant Vaccines against Pasteurella multocida B:2
Abstract: Haemorrhagic septicaemia, caused by Pasteurella multocida serotypes B:2 and E:2, is an acute, fatal and septicaemic disease of bovines occurring as catastrophic epizootics in most tropical regions of Asia and Africa. As chemotherapeutic treatment of P. multocida infections is expensive and ineffective, prevention of disease outbreaks following immunization is the most potent tool. Although, various vaccine formulations as alum precipitated, oil adjuvant, multiple emulsion and live attenuated vaccines are currently …
Published in Research and Reviews : A Journal of Immunology · Vol. 6, Issue 2, 2016 · pp. 1–12 Read article
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Progress from the Traditional Vaccines to Novel Rational Vaccine Designs
Abstract: Vaccines are among the most valuable assets for saving human and animal lives. The vaccines discovered and designed on the Pasteurian principles laid the foundations of the traditional vaccine regime during the late-19th century. Some traditional vaccine designs proved remarkably successful, while others had safety or efficacy problems, and still others failed- particularly those against diseases caused by non-cultivable, hyper-variable or opportunist pathogens, or those requiring cellular immunity. The problems …
Published in Research and Reviews : A Journal of Immunology · Vol. 6, Issue 3, 2016 · pp. 17–31 Read article
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COVID-19: Impact on Human Health
Abstract: Researchers are on the cutting edge of devising the best strategy to combat the SARS-CoV-2 pandemic by studying and exploring the pathogenesis, prevention, and treatment as the world continues to be plagued by an increasing number of confirmed COVID-19 cases. The primary method for detecting the virus is via antigen testing and more accurate molecular tests such as rt PCR using patient samples. The most prevalent treatments for COVID-19 infection …
Published in Research and Reviews : A Journal of Immunology · Vol. 12, Issue 1, 2022 · pp. 1–8 Read article
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The Ames Test in Twenty-first Century
Abstract: Since the 19th century, industrial development has increased exposure to mutagens and possible carcinogens. In order to avoid these health risks, several techniques have been developed to recognize genotoxins. In the 1970s, Dr. Bruce Ames and colleagues, studying the histidine operon of Salmonella typhimurium, observed that histidine (−) auxotroph mutants were reverted to histidine (+) upon exposure to a mutagen. The researchers further observed that known carcinogens were mutagenic to …
Published in Research and Reviews: A Journal of Toxicology · Vol. 2, Issue 1, 2012 · pp. 23–37 Read article
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Evaluation of Marine Waste Water And Aromatic Hydrocarbons Toxicity Using A Battery of Assays
Abstract: Abstract The aim of this study is to evaluate the toxic effects of marine wastewater and representative aromatic hydrocarbons using a battery of assays. The methods employed for the toxicological evaluation involve physic-chemical analysis of the marine wastewater, toxi-chromo test using mutant E coli and sub-chronic animal toxicity testing with emphasis on body weight determination, biochemical indices and histopathological examination. The results showed that Nembe water side wastewater sample contain …
Published in Research and Reviews: A Journal of Toxicology · Vol. 10, Issue 2, 2020 · pp. 1–13 Read article
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Frequency and Effect of CCR5 Alleles on HIV-1 Disease Progression among Untreated Seropositive Individuals Visiting Gondar University Teaching Hospital
Abstract: Abstract:The natural history of HIV-1 infection varies considerably from one individual to another, with some individuals progressing to AIDS rapidly after primary infection, while others remain clinically asymptomatic with no evidence of immune dysfunction. Recent studies have shown that reasons for progression and non-progression are multi-factorial and may involve genetic, virological and immunological factors that influence HIV disease progression in various ways. Chemokine receptors act as important co-receptors mediating the …
Published in Research and Reviews: A Journal of Microbiology and Virology · Vol. 5, Issue 1, 2015 · pp. 8–13 Read article
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Comparative Assessment of Antiproliferative Potential of Triphala and Ashokarishta: Dependence on Dose, Exposure Time and Age of the Preparation
Abstract: Triphala and Ashokarishta are known to be widely used herbal formulations in Ahmedabad, of which a few are local brands. Triphala is used by people of all ages for general well being while Ashokarishta is prescribed to women suffering from problems related to menstruation. Five brands of Tripahala and three brands of Ashokarishta were added to Schizosaccharomyces pombe NCYC 1683 cultures (cell cycle mutants) at concentrations of 0.5, 1.0, 1.5 …
Published in Research and Reviews : A Journal of Biotechnology · Vol. 4, Issue 2, 2014 · pp. 8–16 Read article
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In Silico Stability Analysis and Phosphorylation Induced Structural Simulation of Alpha-synuclein in Parkinson’s disease
Abstract: Alpha-synuclein (α-syn) is the major component of pathologic inclusions that characterizes neurodegenerative disorders such as Parkinson’s disease (PD), dementia with Lewy body disease, and multiple system atrophy. Mutations and phosphorylation of α-syn causes familial PD. Phosphorylation of α-syn at TYR125 and SER129 plays an important role in PD and enhances its partial digestion by calpains leading to its aggregation; however, the exact mechanism is unknown. The present study uses in …
Published in Research & Reviews: A Journal of Bioinformatics · Vol. 2, Issue 1, 2015 · pp. 15–21 Read article
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Methodologies for in silico and in vitro Test of Biphenylalanine Compounds as Potential HIV-1 gp120 Attachment Inhibitors
Abstract: We postulate that biphenylalanine and its derivatives are propitious candidates to inhibit the binding of HIV-gp120 to CD4 T-cell. Many biphenylalanine derivatives are synthesized chemically and also prevailing in nature. We have proposed atom- and bond-based 2D TOMOCOMD-CARDD approach to identify the best derivative. Moreover, according to our proposition, the principal law simulated from in silico interaction of gp120 with CD4 can be tested by ELISA in vitro. Furthermore, the …
Published in Research & Reviews: A Journal of Bioinformatics · Vol. 2, Issue 3, 2015 · pp. 38–42 Read article
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A Method of Universal Primer Design for The Detection of Diverged CTX-M Beta-Lactamases in Multi-Drug Resistant Superbugs
Abstract: Complexity of many diverged β-lactamases in common multi-drug resistant bacteria has reached very high level. We found >40% of common bacteria in Kolkata water bodies were ampicillin resistant and reported clinical isolates were >95% ampicillin resistant worldwide. More than twenty unique sequence classes of β-lactamases were known to cause MDR inactivating penicillin, cephalosporin and carbapenem drugs. Further, there are other diverged MDR genes like drug efflux genes (acrAB, mexAB-EF, tetA), …
Published in Research and Reviews : A Journal of Biotechnology · Vol. 9, Issue 2, 2019 · pp. 1–10 Read article
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Conventional and Molecular Approaches for Development of Quality Protein Maize
Abstract: Maize is a nutritionally poor protein quality crop because of deficient in essential amino acid lysine and tryptophan. Genetic improvement of balanced amino acid profile by application of plant breeding is most viable and sustainable strategies against battling malnutrition opaque-2 mutant is associated with high lysine and tryptophan content and it has widely used by breeder for development of quality protein maize. Adverse effect of opaque-2 genotypes is further improved …
Published in Research & Reviews: A Journal of Pharmacognosy · Vol. 1, Issue 3, 2014 · pp. 12–14 Read article
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DESIGNING OF COUMARIN ANALOGS: A NEW INSIGHT AS ENOL ACYL CARRIER PROTEIN REDUCATSE INHIBITORS
Abstract: Tuberculosis was almost certainly the leading cause of death in Europe and the United States in recorded history, although because of the multifarious forms of the disease. Based on literature we have designed coumarin linked with thiadiazole derivatives as antitubercular agents. To determine structural requirements, we carried out molecular docking study in which we found that our designed molecule have similar binding with enoyl acyl carrier protein reductase (InhA). The …
Published in Research & Reviews: A Journal of Drug Design & Discovery · Vol. 6, Issue 2, 2019 · pp. 1–5 Read article