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649 articles for “isolate”
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Influence of Aeration on Biomass and Polyhydroxyalkanoate Accumulation in Priestia flexa JP1: A Foundation for Nano-Biomaterial Development
Abstract: Polyhydroxyalkanoates (PHAs) are microbial polymers that have attracted substantial interest in recent times due to their use as biodegradable substitutes for petroleum-derived plastics and also as precursors for nano-enabled biomaterials. The aim of the present work was to evaluate the effect of static and shaking culture conditions on growth kinetics, biomass formation, and PHA synthesis by Priestia flexa JP1. The bacterial strain was cultured in mineral salt media (MSM) at …
Published in Research and Reviews : A Journal of Biotechnology · Vol. 16, Issue 2, 2026 · pp. 42–50 Read article
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Molecular Identification and Characterization of a Rice Field Cyanobacterium for its Possible Use as Biofertilizer in Acidic Environment
Abstract: Normal 0 false false false EN-US X-NONE X-NONE /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Table Normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-priority:99; mso-style-qformat:yes; mso-style-parent:""; mso-padding-alt:0in 5.4pt 0in 5.4pt; mso-para-margin:0in; mso-para-margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:11.0pt; font-family:"Calibri","sans-serif"; mso-ascii-font-family:Calibri; mso-ascii-theme-font:minor-latin; mso-fareast-font-family:"Times New Roman"; mso-fareast-theme-font:minor-fareast; mso-hansi-font-family:Calibri; mso-hansi-theme-font:minor-latin; mso-bidi-font-family:"Times New Roman"; mso-bidi-theme-font:minor-bidi;} Initial screening for cyanobacterial diversity in rice fields of Meghalaya revealed presence of a limited number of species predominantly belonging to heterocystous cyanobacteria such as Nostoc and Anabaena. …
Published in Research and Reviews: A Journal of Microbiology and Virology · Vol. 1, Issue 3, 2011 · pp. 1–12 Read article
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Fluconazole Resistance among Candida species Causing Vulvovaginitis
Abstract: Yeasts have emerged as a prominent cause of vaginal infections in community and hospitalized patients. Majority of cases of Vulvovaginal candidiasis (VVC) are caused by Candida albicans however, episodes due to non-albicans Candida appear to be increasing in the recent years leading to increase medical concern. Most non-albicans Candida species have higher minimum-inhibitory concentration (MIC) to fluconazole and infections caused by these species are often difficult to treat. This study …
Published in Research and Reviews: A Journal of Microbiology and Virology · Vol. 2, Issue 2, 2012 · pp. 1–3 Read article
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Amplification and in silico Study of Plasmodium vivax and Plasmodium falciparum LDH Gene
Abstract: Several arsenal as well as quinolone drugs are available for treatment of malaria; still it remains a worldwide public health problem. Malarial parasite quickly develops resistance under selective drug pressure. Hence, we require an array of new drugs for malaria treatment. Lactate dehydrogenase (LDH) is an essential enzyme for Plasmodia as they lack functional Krebs cycle in some stages of their lifecycle and thereby prove to be good targets for …
Published in Research and Reviews: A Journal of Microbiology and Virology · Vol. 2, Issue 3, 2012 · pp. 1–5 Read article
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Identification of Genital Colonization of Group B Streptococci Rate during Late Pregnancy and Its Correlates with Demographic Factors in Bundelkhand Region of Uttar Pradesh, India
Abstract: This study was undertaken to find the GBS colonization rate among women at > 28 weeks of gestation and to assess the type of specimens and techniques used for GBS detection and the demographic factors affecting the carriage in Bundelkhand region of Uttar Pradesh. A total of 434 consecutive vaginal and rectal swabs were obtained from 217 pregnant women at > 28 weeks of gestation and their follow-up testing. Swab …
Published in Research and Reviews: A Journal of Microbiology and Virology · Vol. 3, Issue 2, 2013 · pp. 13–19 Read article
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Detection of Listeria monocytogenes in Milk and Ice Cream by Polymerase Chain Reaction
Abstract: Abstract The present study was undertaken to standardize PCR assay for detection of L. monocytogenes and Listeriolysin O from livestock foods and compare its efficacy with conventional cultural methods. A set of primer derived from iap gene and other set derived from hlyA gene were used for detection of L. monocytogenes and Listeriolysin O in the PCR assay. The PCR assay was initially standardized by optimizing the concentration of the …
Published in Research and Reviews: A Journal of Microbiology and Virology · Vol. 5, Issue 1, 2015 · pp. 24–35 Read article
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Methicillin Resistant Staphylococcus Aureus (MRSA) and Its Different Testing Methods: A Review
Abstract: Staphylococcus aureus, a gram-positive bacterium is a serious current health care concern. In the mid-1980s, S. aureus strains emerged that were resistant to the beta-lactamase-stable penicillin, these strains were termed methicillin resistant S. aureus (MRSA). Methicillin resistance in S. aureus is primarily mediated by the mecA gene, which codes for the modified penicillin-binding protein 2a. MRSA strain has become one of the well-known etiologic agents for a wide variety of …
Published in Research and Reviews: A Journal of Microbiology and Virology · Vol. 6, Issue 1, 2016 · pp. 27–30 Read article
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Production of Bio-ethanol from Waste Materials
Abstract: This work finds the production of bio-ethanol using suitable enzymes and yeast strains. It further tests for the presence of bio-ethanol in the fermented product both quantitatively and qualitatively in the laboratory. Moreover, it aims to evaluate the feasibility of bio-ethanol production from different cellulosic waste materials like wheat straw, waste paper, corn cob, groundnut shell, wood bark, saw dust, bagasse and a combined mixture of all. Two strains of …
Published in Research and Reviews : A Journal of Biotechnology · Vol. 3, Issue 2, 2013 · pp. 33–44 Read article
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Antibacterial Activity of Enicostemma Axillare Against Wound Pathogens
Abstract: The leaves extracts of Enicostemma axillare are used in traditional medicine for treatment of various infections. The antibacterial activity detected was against S.aureus, E.coli, Pseudomonas aeruginosa, P.vulgaris and K.pnemoniae. Wound swabs obtained from ten injured patients at Government hospital, led to the isolation of bacteria using standard methods, the microorganisms were isolated. Then chloroform, methanol and aqueous extract of Enicostemma axillare against S.aureus, E.coli, Pseudomonas aeruginosa, P.vulgaris and K.pnemoniae exhibited …
Published in Research and Reviews : A Journal of Biotechnology · Vol. 4, Issue 3, 2014 · pp. 1–3 Read article
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Starter Culture Development for Spontaneously Fermented Buttermilk
Abstract: Fourteen fermented milks were made from ultra-high temperature (UHT) treated milk using mixtures of three strains of lactic acid bacteria (LAB) and two yeast strains isolated from spontaneously fermented buttermilk (SFB). The isolates used for this experiment were Lactococcus (Lc.) lactis ssp lactis strain NGLC1, Lc. lactis ssp lactis strain NGLC2, Lactobacillus plantarum strain NGLB1, Saccharomyces cerevisiae strain NGY1 and Kluyveromyces marxianus strain NGY2. Inoculated milk was fermented for 48 …
Published in Research and Reviews : A Journal of Biotechnology · Vol. 6, Issue 1, 2016 · pp. 11–25 Read article
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Production of Phosphatase by Phosphate Solubilizing Improved Fungal Strains
Abstract: Phosphate solubilizing micro-organisms were isolated from rhizosphere soils of rice fields around Mannachanalur area, Tiruchirappali district of Tamilnadu. The isolated fungal cultures were identified as Aspergillus niger, Aspergillus fumigatus and Penicillium sp. The efficacy of Phosphatase activity of fungal strains was carried out. The fungal strains were subjected to random mutational studies such as UV and chemical mutation. The Phosphatase assay was carried out after random mutagenesis using wild and …
Published in Research and Reviews : A Journal of Life Sciences · Vol. 2, Issue 1, 2012 · pp. 39–52 Read article
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“Optimization of Physiological Parameters for Production of PUFA using Biodiesel Derived Crude Glycerol as Carbon Sourceâ€
Abstract: Global interest on biodiesel as an alternative fuel has increased over the years, leading to production of large crude glycerol glut, which is impure and has low economic value. Its microbial conversion to value added products like organic acids, alcohol and fatty acid such as polyunsaturated fatty acids (PUFA) can add to the market value to this crude glycerol. The present work reports on the optimization studies on physiological parameters …
Published in Research and Reviews : A Journal of Life Sciences · Vol. 6, Issue 2, 2016 · pp. 1–5 Read article
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Transgenic Expression of Stress Tolerance Gene in Sugarcane (Saccharum officinarum L.)
Abstract: AbstractAgrobacterium tumefaciens LBA4404 mediated transformation of sugarcane variety 31 with two plasmids of pB1121 constructs, one containing PsCBL gene and the other containing PsCIPK gene was studied for stress tolerance capability induction. Calli of the variety Isd 31 were infected with both plasmid containing strains using three different infection times of 60, 90 and 120 min in acetosyringone infection medium. In GUS (β-glucuronidase) assay, randomly selected calli from 120 min …
Published in Research & Reviews : Journal of Agricultural Science and Technology · Vol. 6, Issue 2, 2017 · pp. 32–39 Read article
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Assessment of In Vitro Probiotic Potential of Lactic Acid Bacteria
Abstract: AbstractProbiotic potential of fermented milks isolates Lactobacillus rhamnosus NS6, Streptococcus thermophilus MD2 and Streptococcus thermophilus MD8 were carried out by performing various in vitro tests. MD2 and MD8 were able to survive at pH 2 and 3 in broth, while NS6 was found to be pH sensitive and could not survive at pH 2, but maintained its viability at 3 pH. All the cultures were able to survive at 0.5 …
Published in Research and Reviews : Journal of Dairy Science and Technology · Vol. 5, Issue 1, 2016 · pp. 17–30 Read article
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Strain Improvement of Lactobacillus casei Strain Shirota for Production of Bacteriocin and Its Utilization as a Biopreservative
Abstract: Food is a great concern for humans as well as for animals; also because as we buy vegetables and fruits from the market and after some days with or without refrigeration, they start getting spoiled. To minimize the spoilage and make them preserve for a long period, we did this research on preservation of vegetables and fruits using lactobacillus casei isolated from the Yakult and testing in vitro against spoilage …
Published in Research and Reviews : Journal of Dairy Science and Technology · Vol. 9, Issue 2-3, 2020 · pp. 18–31 Read article
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Paeonia emodi: An Ethnopharmacological and Phytochemical Review
Abstract: This review is an attempt to evaluate the phytochemical potential of the Paeonia emodi and covers the pharmacological and ethnopharmacological activities of its chemical constituents, newly isolated chemical compounds and their medicinal importance. P. emodi is used in several traditional medicinal systems in China, Korea, Pakistan, India and Thailand. Different isolated natural products like anthraquinones, terpenoids, tannins, carbohydrates, phenolics and tannins, glycosides, paeoniflorin, lactiflorin, oxypaeoniflorin, cycloartenol, cholesterol, sitosterol, campesterol, emodinol, …
Published in Research & Reviews : Journal of Herbal Science · Vol. 6, Issue 2, 2017 · pp. 11–20 Read article
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A Comprehensive Review: Medicinal Plants with Potential Antidiabetic Activity
Abstract: AbstractMedicinal plants really play an important role in the treatment of various health problems because of their better acceptance and being safe from undesirable side effects. Diabetes mellitusis is an interesting area of herbal research at present. More than 400 medicinal plants are used to treat diabetes mellitus all over the world. A number of animal studies to test the claimed activity have demonstrated the hypoglycemic property of many of …
Published in Research & Reviews : Journal of Herbal Science · Vol. 4, Issue 1, 2015 · pp. 29–57 Read article
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Growth and Nitrogen (N) Metabolizing Enzymes of Mesophilic and Psychrophilic Heterocystous Cyanobacteria—In Response to Temperature Regimes
Abstract: The increased NR & NiR activity, GS and nitrogenase activities at 25°C and 35°C in psychrophilic and mesophilic organisms indicates that the Arctic isolate is a psychrotolerant as it shows increased enzyme levels than its original temperature in which we isolated the organism though biomass was greater at 3°C. The biomass and enzyme data indicates cyanobacteria are the initiators of primary colonization in polar and alpine environments and are responsible …
Published in Research and Reviews : A Journal of Life Sciences · Vol. 8, Issue 3, 2018 · pp. 31–43 Read article
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Modified Cetyl Trimethyl Ammonium Bromide Method Improves DNA Quality and Quantity in Cotton: The Benchmark for Plant DNA Extraction
Abstract: A wide range of plant genomic DNA extraction or isolation methods are available; however, many of those are limited in their application for a diversified range of plant species. With special emphasis on robustness and versatility, we have improved the cetyl trimethyl ammonium bromide method and isolated high-quality DNA from cotton leaf samples. The major modifications made to the protocol described here were a reduction of sample treatment steps and …
Published in Research and Reviews : A Journal of Life Sciences · Vol. 8, Issue 3, 2018 · pp. 15–19 Read article
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Chemical Constituents from High Altitude Herbs of Kumaun Himalaya: Swertia Purpurascens Wall
Abstract: ABSTRACTA rare triterpenoid queretoric acid from Swertia purpurascens Wall. (Gentianaceae) has been isolated along with β-sitosterol-D-glycoside, oleanolic acid. Except oleanolic acid these two compounds for the first time have been reported from this plant source and identified by means of chemical as well as spectroscopic methods.Keywords: Swertia, queretoric acid, β-sitosterol-D-glycoside, oleanolic acid, isolation
Published in Research & Reviews : Journal of Herbal Science · Vol. 1, Issue 1, 2012 · pp. 24–30 Read article