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Research and Reviews : A Journal of Life Sciences

Modified Cetyl Trimethyl Ammonium Bromide Method Improves DNA Quality and Quantity in Cotton: The Benchmark for Plant DNA Extraction

  1. Ravichandran Mariappan
  2. Subramanyam Koona

Abstract

A wide range of plant genomic DNA extraction or isolation methods are available; however, many of those are limited in their application for a diversified range of plant species. With special emphasis on robustness and versatility, we have improved the cetyl trimethyl ammonium bromide method and isolated high-quality DNA from cotton leaf samples. The major modifications made to the protocol described here were a reduction of sample treatment steps and change in b-mercaptoethanol concentration (to 0.2%) resulting in a robust, rapid, and reproducible plant DNA extraction protocol that can be used for a broad range of plant species and tissue types. Keywords: Cotton, DNA, leaf samples, PCR, SSR markersCite this ArticleRavichandran Mariappan, Subramanyam Koona. Modified Cetyl Trimethyl Ammonium Bromide Method Improves DNA Quality and Quantity in Cotton: The Benchmark for Plant DNA Extraction. Research & Reviews: A Journal of Life Sciences. 2018; 8(3): 15–19p
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