Thymoquinone Induces Epigenetic Modulation in ROS Dependent Manner

Saad Mustafa; Jogendra Singh Pawar; Ilora Ghosh

doi:10.37591/rrjot.v7i1.1323

Abstract

Traditional drugs have the ability to treat broad range of diseases by modulating the signaling pathwaysof inflammatory diseases and cancer. Epigenetic modulation is also one of the mechanisms already established as a process of action. Histone deacetylase (HDAC) inhibition leads to changes in gene expression promoting cell death. Thymoquinone (TQ), a bioactive compound from Nigella sativa seeds has shown to have an antineoplastic effect in many cancers. Here we have investigated HDAC inhibiting potential of TQ. It was observed that (TQ) inhibits the cell viability of HeLa and HepG2 cell lines in concentration dependent manner. It isable to enhanced the total oxidative species in both the cell lines. TQ treatment also causes a loss in mitochondrial membrane potential. The analysis of protein expression of HDAC1 and has shown a decrease in the level of HDAC1 and increase in the p21expressionthat confirming its ability to induce epigenetic modulation. Keywords: Thymoquinone, Epigenetic, ROS, Mitochondrial membrane potential Cite this ArticleSaad Mustafa, Jogendra Singh Pawar, Ilora Ghosh. Thymoquinone Induces Epigenetic Modulation in ROS Dependent Manner. Research and Reviews: Journal of Toxicology. 2017; 7(1): 32–39p.